Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,675,931 A→T 100% Q199L (CAG→CTG)  prfB → peptide chain release factor 2

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,675,9310A→T100.0% 708.7 184Q199L (CAG→CTG) prfBpeptide chain release factor 2
Reads supporting (aligned to +/- strand):  new base (65/119):  ref base (0/0):  total (65/119)

Only 100 of 196 total aligned reads displayed.

ACCGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGACAGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGATC  >  NC_014034/1675834‑1676025
                                                                                                 |                                                                                              
aCCACGGGTGATGAGGCGGGGTTCCTCTCTGTCGCCTACAATATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGt                                                                                             <  2:2285598/101‑1 (MQ=255)
 ccGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTg                                                                                            <  2:2553042/101‑1 (MQ=255)
  cgcgGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGc                                                                                           <  2:926464/101‑1 (MQ=255)
     gggCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCggg                                                                                        >  2:2943097/1‑100 (MQ=255)
         gATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGGGCACCGGCTGGGGGGGGTTTc                                                                                    >  2:3453158/1‑101 (MQ=255)
         ccgatctcGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTc                                                                                    <  2:1532750/94‑1 (MQ=255)
          aTGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGttttg                                                                                   >  1:3129506/1‑99 (MQ=255)
           tGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGc                                                                                  <  2:2163860/101‑1 (MQ=255)
           tGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTTGc                                                                                  >  2:4005202/1‑101 (MQ=255)
              ggCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCt                                                                               <  1:2020252/101‑1 (MQ=255)
               gCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTa                                                                              >  1:4153332/1‑101 (MQ=255)
                cGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTAt                                                                             <  1:278922/101‑1 (MQ=255)
                cGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTcaggtcgg                                                                             >  1:1532854/1‑94 (MQ=255)
                 ggggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATg                                                                            <  2:2254296/101‑1 (MQ=255)
                 ggggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATg                                                                            <  1:1365340/101‑1 (MQ=255)
                 ggggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATg                                                                            >  2:416071/1‑101 (MQ=255)
                   ggATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGAc                                                                          <  1:1163835/101‑1 (MQ=255)
                    gATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACt                                                                         <  2:2486214/101‑1 (MQ=255)
                    gATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACt                                                                         <  2:2546283/101‑1 (MQ=255)
                    gATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTTGCCCCATGACt                                                                         >  1:3346818/1‑101 (MQ=255)
                     acccGCCCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAAGCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTc                                                                        <  2:1903114/99‑1 (MQ=255)
                     aTCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGACTc                                                                        >  2:349433/1‑101 (MQ=255)
                     aTCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTc                                                                        <  2:2014386/101‑1 (MQ=255)
                     aTCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTTGCCCTATGACTc                                                                        >  1:3270179/1‑101 (MQ=255)
                     aTCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTTGCCCCATGACTc                                                                        >  1:1632380/1‑101 (MQ=255)
                      tCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACttg                                                                       >  1:4187685/1‑99 (MQ=255)
                      tCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTcg                                                                       <  2:792713/101‑1 (MQ=255)
                         gCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTcggcg                                                                    <  1:78168/101‑1 (MQ=255)
                          cTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGTTTCGCCCTATGACTcggcgg                                                                   >  1:2949071/1‑101 (MQ=255)
                            cGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTTGCCCTATGACTCCgcggcg                                                                 >  1:873318/1‑101 (MQ=255)
                             ggTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGCCCTATGGCTCGgcggggc                                                                >  2:3781881/1‑101 (MQ=255)
                             ggTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGgcggcgg                                                                >  1:1816860/1‑100 (MQ=255)
                              gTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTTGCCCTATGACTCGgcggcgcg                                                               >  2:1491545/1‑101 (MQ=255)
                              gTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGgcggcgcg                                                               <  2:4182696/101‑1 (MQ=255)
                                cGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGgcggcgcggc                                                             <  2:2179416/101‑1 (MQ=255)
                                   cTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGACTCGGCGGCGcggcggg                                                          >  1:390496/1‑100 (MQ=255)
                                   cTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGGATTCGGCCTATGACTCGGCGGCGCcgcggc                                                          >  1:3247659/1‑101 (MQ=255)
                                    tACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTCTGACTCCGGGGGGCGGCGGCa                                                         >  2:3575490/1‑101 (MQ=255)
                                       aaGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGGGCGGCGGCCTAc                                                      >  2:2356681/1‑101 (MQ=255)
                                       aaGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAc                                                      <  2:1816740/101‑1 (MQ=255)
                                       aaGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAc                                                      <  1:1236772/101‑1 (MQ=255)
                                        aGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcg                                                     >  1:228475/1‑101 (MQ=255)
                                            ttCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtcgt                                                 >  2:4198182/1‑101 (MQ=255)
                                             tCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGtt                                                <  1:1970430/101‑1 (MQ=255)
                                             tCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGtt                                                <  2:3943701/101‑1 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGGTTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGGCGTTc                                               >  2:3223409/1‑101 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATAcgtctgtc                                               >  2:1106907/1‑97 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTc                                               <  1:885260/101‑1 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTc                                               <  2:3000640/101‑1 (MQ=255)
                                              ccGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGGCGTTc                                               >  1:3501905/1‑101 (MQ=255)
                                               cGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCa                                              <  1:1088201/101‑1 (MQ=255)
                                               cGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCa                                              <  1:3305051/101‑1 (MQ=255)
                                                ttccgatctGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAg                                             <  1:2815686/93‑1 (MQ=255)
                                                 ggCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGc                                            <  1:345590/101‑1 (MQ=255)
                                                  gCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTCCACCGGCTGGGGCGGATTTCTCCCTATGACTCGGCGGCGGGGCGGCATCCGTCCTTCaacg                                           >  1:1269277/1‑98 (MQ=255)
                                                    aGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCCGCTCg                                         >  1:617578/1‑101 (MQ=255)
                                                    aGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCg                                         <  1:416122/101‑1 (MQ=255)
                                                     gAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCCCgg                                        >  1:246480/1‑101 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCCTACGTCGTTCAgagatcggaa                                    >  2:2815467/1‑92 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTg                                    <  2:2680413/101‑1 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTg                                    <  2:1287427/101‑1 (MQ=255)
                                                         gCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGGCGTTCCGCTCGGTCTg                                    >  1:222058/1‑101 (MQ=255)
                                                             aTGGCTGGCTGAAATCGGAATCGGGCGTGCACCGGCTGGGGCGGATTTCGCCCTATGACTCGGCGGCGCGGGGGCATACGGCGGTCAGCTCGGTCTgggcc                                >  1:1011558/1‑99 (MQ=255)
                                                              tggctggcTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCt                               <  2:3770621/101‑1 (MQ=255)
                                                              tggctggcTGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCt                               <  2:1459624/101‑1 (MQ=255)
                                                               ggctggctGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTa                              <  2:2890224/101‑1 (MQ=255)
                                                               ggctggctGAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTa                              <  2:1853663/101‑1 (MQ=255)
                                                                 ctggctgAAATCGGAATCGGTCCTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATc                            <  2:1421760/101‑1 (MQ=255)
                                                                 ctggctgAAATCGGAATCGGGCGTGCACAGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGGCTATc                            >  2:2450241/1‑101 (MQ=255)
                                                                  tggctgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATcc                           <  1:4005462/101‑1 (MQ=255)
                                                                     ctgAAAACCGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGGCTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgt                        <  2:1131287/101‑1 (MQ=255)
                                                                      tgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtc                       <  1:1117386/101‑1 (MQ=255)
                                                                      tgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtc                       <  1:1478462/101‑1 (MQ=255)
                                                                      tgAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtc                       <  2:1668309/101‑1 (MQ=255)
                                                                       gAAATCGGAATCGGGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtcg                      <  1:705025/101‑1 (MQ=255)
                                                                         aatcggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGgtcgtc                    <  1:430533/101‑1 (MQ=255)
                                                                          atcggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGtcgtcg                   <  1:2623950/101‑1 (MQ=255)
                                                                          atcggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGtcgtcg                   <  2:3545383/101‑1 (MQ=255)
                                                                             ggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgacg                <  1:2863942/101‑1 (MQ=255)
                                                                             ggaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgacg                >  1:3673257/1‑101 (MQ=255)
                                                                              gaatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTcgacga               >  1:371094/1‑101 (MQ=255)
                                                                               aatcggGCGTGCACCGGCTGGTGCGGATTTCGCTCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCgacgac              <  1:3630960/101‑1 (MQ=255)
                                                                               aatcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCgacgac              >  2:1254928/1‑101 (MQ=255)
                                                                                 tcggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACaa            <  2:3856275/101‑1 (MQ=255)
                                                                                  cggGCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAat           <  1:3095862/101‑1 (MQ=255)
                                                                                   gggCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAata          <  2:534836/101‑1 (MQ=255)
                                                                                   gggCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAata          <  2:2765438/101‑1 (MQ=255)
                                                                                     gCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATc        <  2:3654894/101‑1 (MQ=255)
                                                                                     gCGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATc        <  1:2699130/101‑1 (MQ=255)
                                                                                      cGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGCCGACAATATCg       >  1:1594861/1‑101 (MQ=255)
                                                                                      cGTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCg       <  2:1183540/101‑1 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      >  1:3211324/1‑101 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      >  1:2532071/1‑101 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      <  2:826081/101‑1 (MQ=255)
                                                                                       gTGCACCGGCTGGTGCGGATTTCGCCCTATGACACGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCga      >  1:4209620/1‑101 (MQ=255)
                                                                                         gCACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCgaga    >  1:3538050/1‑101 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   <  1:3819887/101‑1 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   >  2:3687805/1‑101 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   <  1:2441640/101‑1 (MQ=255)
                                                                                          cACCGGCTGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGAt   <  1:3392241/101‑1 (MQ=255)
                                                                                                 |                                                                                              
ACCGCGGGCGATGAGGCGGGGATCCGCTCGGTCGCCTACAAGATTTCCGGGCAGAATGCCTATGGCTGGCTGAAATCGGAATCGGGCGTGCACCGACAGGTGCGGATTTCGCCCTATGACTCGGCGGCGCGGCGGCATACGTCGTTCAGCTCGGTCTGGGTCTATCCGGTCGTCGACGACAATATCGAGATC  >  NC_014034/1675834‑1676025

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG