Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,951,208 G→C 89.6% A129G (GCG→GGG)  ssb1 ← single‑stranded DNA‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,951,2080G→C89.6% 26.3 ‑0.6 0.0115A129G (GCG→GGG) ssb1single‑stranded DNA‑binding protein
Reads supporting (aligned to +/- strand):  new base (64/39):  ref base (6/6):  total (70/45)
Fisher's exact test strand distribution p-value = 5.34e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

GCCCGACGAGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.C.GTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299
                                                                                                |                                                                                             
gccCGACGAGGGGGCC.GAGGAG.CCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAAt                                                                                       <  2:945156/101‑1 (MQ=255)
 cccGACGAGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAAtt                                                                                      >  2:4063178/1‑101 (MQ=255)
  ccGACGAGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTg                                                                                     >  2:1461955/1‑101 (MQ=255)
   cgacgaGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGc                                                                                    >  1:530285/1‑101 (MQ=255)
   cgacgaGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGc                                                                                    >  2:4126671/1‑101 (MQ=255)
   cgacgaGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATGGc                                                                                    >  2:3878292/1‑101 (MQ=255)
    gacggGGGGGCG.GGGGAG.CCCCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGcc                                                                                   <  2:2810184/101‑1 (MQ=255)
    caacggggggCC.GGGGGGCCCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGc                                                                                    <  2:1290260/96‑1 (MQ=255)
     acggGGGGGCG.GAGGAC.CCGCCGCCCCCGCCGCCAAAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCa                                                                                  <  1:3404411/101‑1 (MQ=255)
      cgaGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAc                                                                                 >  2:2438994/1‑101 (MQ=255)
       gggggggCG.GGGGGG.CCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAcc                                                                                <  1:4118340/99‑1 (MQ=255)
       gggggggCG.GGGGAC.CCCCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAcc                                                                                <  1:602537/99‑1 (MQ=255)
       gaGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAcc                                                                                >  1:2499303/1‑101 (MQ=255)
        gggggcCC.GGGGAC.CCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAccg                                                                               <  2:2330836/100‑1 (MQ=255)
         gggggCG.GAGGAC.CCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAccgc                                                                              <  2:2935121/101‑1 (MQ=255)
         gggggCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAccgc                                                                              >  2:2533037/1‑101 (MQ=255)
         gggggCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCCGCGAACCGCCGC.C.GTAATTGCCAccgc                                                                              >  1:346987/1‑101 (MQ=255)
          ggggCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCAccgcc                                                                             >  1:2412100/1‑101 (MQ=255)
           gggCCGGAGGAG.CCCCCGCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCAccgcc                                                                             <  1:1038937/101‑1 (MQ=255)
           gggCC.GTGGAC.CCCCCCCCACCCCCGCCATAACCCCGGCCGTCTTTGTCCCCCCCCCCATCGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCAccgccg                                                                            <  1:3449183/101‑1 (MQ=255)
              cc.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACcgccgccc                                                                         >  2:3252379/1‑99 (MQ=255)
               gcggagcacccgcccccaccgccaccaCACCCCGGGCCGACATCGTACCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACcgccgcc                                                                          <  2:747097/92‑2 (MQ=255)
               c.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCcgcccg                                                                        >  2:3962979/1‑101 (MQ=255)
               c.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCAGCGAACCGCCGC.C.GCAATTGCCACCGCcgcccg                                                                        >  2:1950186/1‑101 (MQ=255)
                 gaggag.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGa                                                                       >  1:2948824/1‑101 (MQ=255)
                  aggag.CCGCCGCCACCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTACTTGCCACcgccgccccag                                                                      >  1:1951721/1‑96 (MQ=255)
                   ggag.CCGCCCCCCCCGCCGCCATAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgc                                                                     <  1:1786363/101‑1 (MQ=255)
                         cgccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCCCCGGCCGAgccgccg                                                                >  2:3699420/1‑101 (MQ=255)
                         cgcccccccccccgccaTAGCCCGGGCCGTCACCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccg                                                                <  1:2208577/101‑1 (MQ=255)
                         ccccccccccgccgcaaTACCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccg                                                                <  2:1206448/93‑1 (MQ=255)
                          gccgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccgc                                                               >  2:1767638/1‑101 (MQ=255)
                           ccccccccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAgccgccgcc                                                              <  1:3442176/95‑1 (MQ=255)
                            cgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                             >  1:3271174/1‑101 (MQ=255)
                            cgccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCa                                                             >  1:1812277/1‑101 (MQ=255)
                             gccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCAt                                                            >  2:3771036/1‑101 (MQ=255)
                             gccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCAt                                                            >  1:304852/1‑101 (MQ=255)
                             gccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCCAGCCGCCGCCAt                                                            >  2:614800/1‑101 (MQ=255)
                              cccccgccgccaTAGCCGGGGCGGCCCGCGTGGCCGCCGCCATAGCCGCTGCCCTGGGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           <  2:630944/98‑1 (MQ=255)
                              ccaccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATc                                                           >  1:2878341/1‑101 (MQ=255)
                               caccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATGGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATcc                                                          >  2:2162577/1‑101 (MQ=255)
                               caccgccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATcc                                                          >  1:677044/1‑101 (MQ=255)
                                 ccgcccccaTAGCCCGGGCCGTCATCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCg                                                        <  1:351740/101‑1 (MQ=255)
                                   gccgccaTAGCCCGGGCCTTCATCGTGGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgc                                                      <  2:1023599/101‑1 (MQ=255)
                                    ccgccaTGGCCGGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     <  2:209664/101‑1 (MQ=255)
                                    ccgccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgcc                                                     >  1:2609414/1‑101 (MQ=255)
                                      gccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                   >  1:1605078/1‑101 (MQ=255)
                                      gccaTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGgccgc                                                   >  2:1917563/1‑101 (MQ=255)
                                        caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                 >  1:1513991/1‑101 (MQ=255)
                                        caTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCa                                                 >  1:352939/1‑101 (MQ=255)
                                         caaGCCCGGGCCTTCATCGTAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                <  2:3786364/99‑1 (MQ=255)
                                         atggCCCGGCCCTTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                <  2:2163494/98‑1 (MQ=255)
                                         aTAGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCaa                                                >  2:2771440/1‑101 (MQ=255)
                                          tAGGCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAag                                               >  2:3584485/1‑101 (MQ=255)
                                           aGCCCGGGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTCCCACCGCCGCCCGAGCCCCCGCCATCCCGGCCGCCaccc                                              >  1:10601/1‑98 (MQ=255)
                                            gccggggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagca                                             <  1:2959427/95‑1 (MQ=255)
                                            gCCCGGGCCGCCCTCGAAGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagca                                             <  1:3287990/101‑1 (MQ=255)
                                              ccGGGCCGTCCTCGTGGCCCCCGCCATGGCCCCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAagcagc                                           <  2:2141351/101‑1 (MQ=255)
                                               cgggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCACGCAGCg                                          >  1:1749635/4‑101 (MQ=255)
                                               cgggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCg                                          >  2:2448759/4‑101 (MQ=255)
                                                gggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                         >  1:1713678/3‑101 (MQ=255)
                                                gggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                         >  2:1686828/3‑101 (MQ=255)
                                                gggcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGt                                         >  2:493183/3‑101 (MQ=255)
                                                  gcCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCa                                       >  2:2938637/1‑101 (MQ=255)
                                                  gcCGTAACCGTAGCCGCCGCCATAGCCGCCGCCCGGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCa                                       <  1:3318073/101‑1 (MQ=255)
                                                     gTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAggg                                    >  2:3621022/1‑100 (MQ=255)
                                                      tcttCGTAGCCCCCGCCATAGCCGCCGCCCGGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   <  1:1917689/98‑1 (MQ=255)
                                                      tcctCGTACCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGtt                                   <  1:2068980/98‑1 (MQ=255)
                                                       caaggTAGCCGCCACCACACCCGCCCCCCTAC.AACCCCCCG.CAGTAATTGCCACCGCCGCCCGAGCCGCCGCCCTCCCGGCCGCCAAGCAGCGTCAGGTTg                                  <  2:1951595/97‑1 (MQ=255)
                                                       cATCTTGGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTg                                  <  1:600364/101‑1 (MQ=255)
                                                        aTCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGc                                 >  1:433174/1‑101 (MQ=255)
                                                         tCGTAGCCGCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                                <  1:3137623/101‑1 (MQ=255)
                                                         tCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                                >  1:1013693/1‑101 (MQ=255)
                                                         tCGTACCCCCCGCCATAGCCGCCGCCCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                                <  1:12361/101‑1 (MQ=255)
                                                         ccGTGGCCCCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGcc                                <  1:3314616/100‑1 (MQ=255)
                                                          cGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               >  2:1717982/1‑101 (MQ=255)
                                                          cGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGccc                               >  2:1535902/1‑101 (MQ=255)
                                                            tAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                             >  2:2615329/1‑101 (MQ=255)
                                                            tAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGt                             >  1:1824501/1‑101 (MQ=255)
                                                            tAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCCGA.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCCGCGACAGAATGCCCGt                             >  2:1780065/1‑101 (MQ=255)
                                                               ccgcccccATAGCCGCCGCCCGGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtata                          <  2:1513887/101‑1 (MQ=255)
                                                               ccccccccATAGCCGCCCCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGtata                          <  1:2439178/95‑1 (MQ=255)
                                                                cgccgccCAAGCCGCCCCCGTGCAACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAg                         <  2:414951/101‑1 (MQ=255)
                                                                 gccgccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgg                        >  1:2283683/1‑101 (MQ=255)
                                                                  ccgccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAggg                       <  2:3400669/101‑1 (MQ=255)
                                                                   cgccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAgggg                      >  1:3182731/1‑101 (MQ=255)
                                                                    gccATAGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgc                     >  2:3259641/1‑101 (MQ=255)
                                                                      cATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   >  2:4091837/1‑101 (MQ=255)
                                                                      cATAGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGgcgc                   >  2:2958711/1‑101 (MQ=255)
                                                                         aGCCGCCGCCCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                >  1:780040/1‑101 (MQ=255)
                                                                         aGCCGCCGCCCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagc                >  2:3566179/1‑101 (MQ=255)
                                                                          gccgccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagca               >  1:1562794/1‑101 (MQ=255)
                                                                          gccgccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagca               >  1:3100784/1‑101 (MQ=255)
                                                                          gccgccgccCTGCGAACCGC.CGCC.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCgcagca               >  2:1064819/1‑101 (MQ=255)
                                                                              ccccccTGCGAACCGCCGC.C.GTAATTGCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGcaccac           <  1:1462057/98‑1 (MQ=255)
                                                                               cgccCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGGCAGGTGGCCCGTATAGGGGGGCAGCaccacc          >  2:4050007/1‑101 (MQ=255)
                                                                                gccCTGCGACCCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTGAGGTTGCCCGTATAGGGGCGCAGCACCACCt         <  1:3771293/101‑1 (MQ=255)
                                                                                gccCTGCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCt         <  2:2411924/101‑1 (MQ=255)
                                                                                    tGCGACCCGCCGC.C.GTAATTGCCCCCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGt     <  1:536488/101‑1 (MQ=255)
                                                                                     gCGAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTc    >  2:3452705/1‑101 (MQ=255)
                                                                                       gAACCGCCGC.C.GTAATTGCCACCGCCGCCCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCgt  >  1:2743308/1‑101 (MQ=255)
                                                                                                |                                                                                             
GCCCGACGAGGGGGCC.GAGGAG.CCGCCGCCACCGCCGCCATAGCCCGCGCCGTCATCGTAGCCGCCGCCATAGCCGCCGCCCTGCGAACCGCGCG.C.GTAATTGCCACCGCCGCGCGAGCCGCCGCCATCCCGGCCGCCAAGCAGCGTCAGGTTGCCCGTATAGGGGCGCAGCACCACCTCGGTCGT  >  NC_014034/1951114‑1951299

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG