Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,965,271	C→G	100%	intergenic (+39/+53)	bdhA → / ← RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,965,271	0	C→G	100.0%	35.9	14	intergenic (+39/+53)	bdhA/RCAP_rcc01818	3‑hydroxybutyrate dehydrogenase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (4/10):  ref base (0/0):  total (4/10)

GCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGCT.CCG.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCT  >  NC_014034/1965176‑1965364                                                                                                    |                                                                                                 gcggcgCAGATCAGCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC...CGGGcgg                                                                                            >  2:907212/1‑99 (MQ=39)   ggcgCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGCT.cc                                                                                          >  2:1274787/1‑101 (MQ=255)      gcAGATCACCGGCACGACGCTTTCTGTGGATGGCGGCTGGACGGCGGGGTGAGAGATTTA.GCCTCGGGGCAAGCCCGAGGCAGCCCG.GC..GGGGGcctgccc                                                                                        >  2:1319465/1‑95 (MQ=255)      gcAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCggggggncgc                                                                                        >  1:3462983/1‑94 (MQ=255)        aGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGGG.GCG.c.ccc                                                                                   >  1:2705529/1‑101 (MQ=38)              ccGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..CCGGGGG.GCG.c.cccccgcgc                                                                             >  1:1178794/1‑95 (MQ=37)                       cgaTTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCGCCC..GGGGGTT.CCC.C.CCCCGGccccccggggc                                                                     >  2:1218062/1‑95 (MQ=25)                            tCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGCT.CCGCC.CCCCGGCCCCCCAGGGATTTCa                                                                >  2:2595796/1‑101 (MQ=21)                                    ggggggcGGGGCGGCGCTGGGAG.TATTGCGGGCGCGGGCCAGGCCCGAGGGCCCCCG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGAt                                                        <  1:559281/97‑1 (MQ=2)                                             gccGGCGCTGGGAGATTTCGC.CCCGGGGGGCACGCCCGAGCCCCCCCG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAggg                                               <  2:459841/99‑1 (MQ=9)                                                   gttgtgAGAGTTTCC.GCCGCGGCGCACGCCCGGGCCCCCCCG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTc                                         <  2:2705323/99‑1 (MQ=16)                                                         agagaTTTC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CCGGGGGGGCC.CCC.C.CCCGGCCCCCCCGGGGGTTTTGGGCAGAAGAAAAGAGGGGGGC.CAggcgg                                   >  2:2464724/1‑101 (MQ=2)                                                                   gcCTCGGGGCAAGCCCGAGGCGACCCG.CC..GGGGGCTCCCC.C.CCCCCGCCCCCCCGGGGTTTTAGGCAAGAAGAAAGAGGGGCGGCCAGGCGGCCtgcttct                          >  2:629683/1‑100 (MQ=12)                                                                     ggggggccgcgCCCGGGGGGCCCCG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTcgcc                        <  1:2796953/91‑1 (MQ=21)                                                                              ggCCCGGGCCGCCCCG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGccc               <  2:4075643/100‑1 (MQ=25)                                                                                  gggccccccccG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGgtg           <  1:2247624/93‑1 (MQ=25)                                                                                    gccccccccG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtc         <  1:1711898/95‑1 (MQ=25)                                                                                    agcagtcacg.cc..gaGGGCT.CCG.CGCCCGGCCCCCCGAGGTATTTCAGGCAAGACGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTACCCGgtgcc         <  2:4091640/93‑3 (MQ=255)                                                                                      cccccccG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGtctc       <  1:1218156/97‑1 (MQ=25)                                                                                        cccccG.CC..GGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCgg     <  2:433460/99‑1 (MQ=255)                                                                                           ccg.cc..gGGGGCT.CCGCC.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGct  <  1:373268/101‑1 (MQ=255)                                                                                                    |                                                                                                 GCGGCGCAGATCACCGGCACGACGATTTCTGTGGATGGCGGCTGGACGGCGCTGTGAGAGATTGC.GCCTCGGGGCAAGCCCGAGGCGACCCG.CC..GCGGGCT.CCG.C.CCCGGACCCCCGAGGTATTTCAGGCAAGATGAAAGAGGGGCGCTCAGGCGGCCTTCTTCGCCGCCTTGCCCGGTGTCTCGGGCT  >  NC_014034/1965176‑1965364 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG