Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,307,864 G→C 85.5% N311K (AAC→GCC RCAP_rcc02140 ← extracellular ligand‑binding receptor

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,307,8640G→C85.5% 50.6 ‑2.0 0.0141N311K (AAC→AAGRCAP_rcc02140extracellular ligand‑binding receptor
Reads supporting (aligned to +/- strand):  new base (65/53):  ref base (15/5):  total (83/58)
Fisher's exact test strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 146 total aligned reads displayed.

TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGAA  >  NC_014034/2307772‑2307954
                                                                                             |                                                                                           
tAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTcgccgc                                                                                    >  2:3987136/1‑101 (MQ=255)
 aGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCgccgcc                                                                                   <  2:1924322/101‑1 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGccgccg                                                                                  <  1:4210055/101‑1 (MQ=255)
  gCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGccgccg                                                                                  >  1:205296/1‑101 (MQ=255)
    tGGAACGGGTCGGGTTGGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGa                                                                                <  1:1890399/101‑1 (MQ=255)
    tGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGa                                                                                >  2:1349710/1‑101 (MQ=255)
     ggAACGGTTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGAt                                                                               >  2:872840/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:3859222/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:4163625/1‑101 (MQ=255)
       aaCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATcc                                                                             >  2:1262408/1‑101 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCa                                                                            >  2:241369/1‑101 (MQ=255)
        aCGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCa                                                                            >  1:3476400/1‑101 (MQ=255)
        aCGGGCCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCa                                                                            <  2:1766596/101‑1 (MQ=255)
         ggggtcgggtTCGGCCCCGCCGCGGCCAGCCCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAg                                                                           <  1:4197833/100‑1 (MQ=255)
         cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAg                                                                           <  2:4139148/101‑1 (MQ=255)
         cgggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAg                                                                           <  1:4067298/101‑1 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGTCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgc                                                                          <  1:3604201/101‑1 (MQ=255)
          gggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgc                                                                          >  2:3643517/1‑101 (MQ=255)
           ggtcgggtTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAgcg                                                                         >  2:159939/1‑101 (MQ=255)
                   tCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGCgc                                                                 >  1:3952484/1‑101 (MQ=255)
                    cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                                >  2:113646/1‑101 (MQ=255)
                    cGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAgcg                                                                >  2:807047/1‑101 (MQ=255)
                       ccccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccg                                                             <  1:1349810/101‑1 (MQ=255)
                        cccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccgc                                                            >  1:2308011/1‑101 (MQ=255)
                        cccGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGcgccgc                                                            >  2:269380/1‑101 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCGGATAGCCCTCGAAGGAGACCAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                          <  1:3613624/101‑1 (MQ=255)
                          cgccgcGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCt                                                          >  1:4049705/1‑101 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                       <  1:309319/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                       <  2:3567290/101‑1 (MQ=255)
                             cgcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGAt                                                       >  2:1854640/1‑101 (MQ=255)
                              gcgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATa                                                      >  2:745510/1‑101 (MQ=255)
                               cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                     <  1:3016907/101‑1 (MQ=255)
                               cgGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAg                                                     <  1:3067016/101‑1 (MQ=255)
                                ggCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgc                                                    >  1:188371/1‑101 (MQ=255)
                                 gccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcg                                                   <  1:226863/101‑1 (MQ=255)
                                  ccagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgc                                                  >  2:2156048/1‑101 (MQ=255)
                                   cagcgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAgcgcg                                                 <  1:241414/101‑1 (MQ=255)
                                      cgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcca                                              <  2:2532583/101‑1 (MQ=255)
                                      cgccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGcca                                              >  1:2905575/1‑101 (MQ=255)
                                       gccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccac                                             <  1:3836784/101‑1 (MQ=255)
                                       gccgccagcgccAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccac                                             <  2:2400814/101‑1 (MQ=255)
                                         cgccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacca                                           >  2:152784/1‑101 (MQ=255)
                                         cgccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGccacca                                           <  1:1482537/101‑1 (MQ=255)
                                           ccagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCaccacc                                         >  2:3633570/1‑101 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        >  2:2474268/1‑101 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        >  2:3096238/1‑101 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        <  1:3819617/101‑1 (MQ=255)
                                            cagcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCg                                        >  2:1438304/1‑101 (MQ=255)
                                             agcgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCgg                                       >  2:3576415/1‑101 (MQ=255)
                                               cgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGaa                                     <  1:1116570/101‑1 (MQ=255)
                                               cgccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGaa                                     >  2:853202/1‑101 (MQ=255)
                                                gccagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAAt                                    <  1:1158081/101‑1 (MQ=255)
                                                  cagACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGt                                  >  2:1712707/1‑101 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGGAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                                 <  1:3643770/101‑1 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                                 >  1:175042/1‑101 (MQ=255)
                                                   agACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTc                                 >  1:912734/1‑101 (MQ=255)
                                                      cGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCggg                              >  2:750007/1‑100 (MQ=255)
                                                      cGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgt                              <  2:3236551/101‑1 (MQ=255)
                                                      cGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgt                              >  1:630382/1‑101 (MQ=255)
                                                        gCCGGTCAGATAGCCCTCGAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtgt                            >  1:781193/1‑101 (MQ=255)
                                                        gCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGgtgt                            >  1:370908/1‑101 (MQ=255)
                                                         ccGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                           <  2:3413883/101‑1 (MQ=255)
                                                         ccGGTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTc                           <  1:853283/101‑1 (MQ=255)
                                                          cGGTCAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGNTAGCGCGCCACCACCGGAATGTCGGTGTCg                          >  1:194490/1‑101 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                         <  1:3987396/101‑1 (MQ=255)
                                                           ggTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGa                         >  2:756258/1‑101 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                        <  1:554992/101‑1 (MQ=255)
                                                            gTCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGaa                        <  1:2763859/101‑1 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                       <  1:2350179/101‑1 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                       <  2:204495/101‑1 (MQ=255)
                                                             tCAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAAc                       <  2:2438800/101‑1 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                      <  1:3659365/101‑1 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                      >  1:1253578/1‑101 (MQ=255)
                                                              cAGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACg                      >  1:3429279/1‑101 (MQ=255)
                                                               aGATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                     >  1:1424183/1‑101 (MQ=255)
                                                               aGATAGCCCTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACgg                     >  1:2920785/1‑101 (MQ=255)
                                                                gATAGCCCTCGAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGACTGTCGGTGTCGAACGGa                    >  2:3807772/1‑101 (MQ=255)
                                                                gATAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGa                    <  1:745587/101‑1 (MQ=255)
                                                                 aTAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGAt                   <  2:1227527/101‑1 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCCCCGGAATGTCGGTGTCGAACGGATa                  <  2:1319261/101‑1 (MQ=255)
                                                                  tAGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATa                  <  1:2045203/101‑1 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg                 >  1:1351995/1‑101 (MQ=255)
                                                                   aGCCCTCGAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAg                 <  2:782795/101‑1 (MQ=255)
                                                                     cccTCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAggg               >  2:3937846/1‑101 (MQ=255)
                                                                        tCGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCAc            >  2:1432891/1‑101 (MQ=255)
                                                                         cGAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACg           >  2:3439344/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAGCCCGGC..TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  2:2982759/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGGCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:2807891/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCGCGa          >  2:3605262/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          <  1:1262504/101‑1 (MQ=255)
                                                                          gAAGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:1632646/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  1:1110896/1‑101 (MQ=255)
                                                                          gAAGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGa          >  2:3680354/1‑101 (MQ=255)
                                                                           aaGGAGACGAAG.CC.CGGCTTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAc         >  1:3713423/1‑101 (MQ=255)
                                                                            aGGAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCAACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGAcc        >  1:3864106/1‑101 (MQ=255)
                                                                             ggAGACGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCt       <  2:1813478/101‑1 (MQ=255)
                                                                              gagaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTg      >  2:3289550/1‑101 (MQ=255)
                                                                               ggaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGc     <  2:3326436/100‑1 (MQ=255)
                                                                               agaCGAAG.CCCGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGACAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGGTAGGGCACGACCGGc     >  2:3784458/1‑101 (MQ=255)
                                                                                  cGAAG.CCGGGC.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGaa  <  2:767950/101‑1 (MQ=255)
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TAGCTGGAACGGGTCGGGTTCGGCCCCGCCGCGGCCAGCGCCGCCAGCGCCAGACGGCCGGTCAGATAGCCCTCGAAGGAGACGAAG.CCGCCG.TTCGCCGCCGGATCCAGCGCCGCGAGCGCCGCCTGATAGCGCGCCACCACCGGAATGTCGGTGTCGAACGGATAGGGCACGACCTGCGAA  >  NC_014034/2307772‑2307954

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG