Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,559,923 G→C 100% intergenic (‑199/+71) ispZ ← / ← ftsY intracellular septation protein A/cell division protein FtsY

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,559,9230G→C100.0% 384.5 106intergenic (‑199/+71)ispZ/ftsYintracellular septation protein A/cell division protein FtsY
Reads supporting (aligned to +/- strand):  new base (96/10):  ref base (0/0):  total (96/10)

Only 100 of 146 total aligned reads displayed.

TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021
                                                                                                     |                                                                                                    
tcgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTG.G.CGCGTCAGCGCAAgcgg                                                                                                    >  2:936945/1‑101 (MQ=255)
tcgCCCGGTGCGGTTTCCTGACAGGATCGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAgcgg                                                                                                    >  2:3742015/1‑101 (MQ=255)
 cgCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAgcggg                                                                                                   >  1:208660/1‑101 (MQ=255)
   cccGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAgcggggg                                                                                                 <  2:3744236/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               <  1:2428686/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               <  1:1732362/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               >  2:2981355/1‑101 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               <  2:1454235/101‑1 (MQ=255)
     cGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               >  2:1521836/1‑101 (MQ=255)
     cGGTGCGGTTTACTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGc                                                                                               >  2:2718039/1‑101 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAgc                                                                                            <  1:3358755/101‑1 (MQ=255)
        tGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAgc                                                                                            >  2:3216171/1‑101 (MQ=255)
         gCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGTGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAgcg                                                                                           >  2:2844044/1‑101 (MQ=255)
          cGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAgcgc                                                                                          >  1:3906194/1‑101 (MQ=255)
            gTTTCCTGACAGGATTTTGTCCTTCGCGCCCCATCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccc                                                                                        <  1:387683/101‑1 (MQ=255)
             tttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGcccc                                                                                       >  2:3148481/1‑101 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                      >  2:220582/1‑101 (MQ=255)
              ttCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc                                                                                      >  1:1705889/1‑101 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc.c                                                                                    <  1:2723177/101‑1 (MQ=255)
               tCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc.c                                                                                    >  1:1333639/1‑101 (MQ=255)
                 cTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGccccc.cga                                                                                  >  1:3692509/1‑99 (MQ=255)
                    aggggtttttgtCCTTCGCGCCGCAGGCGCCCCG.TTTTTCTTGGGGGAAAGCGCCGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGC.CCCCC.CCG.AAgc                                                                             <  2:3767526/92‑1 (MQ=255)
                     cAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAgcg                                                                            >  2:1866157/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.T.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAgcgc                                                                           >  1:3744546/1‑101 (MQ=255)
                      aGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAgcgc                                                                           >  1:118300/1‑101 (MQ=255)
                       ggATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGcc                                                                          >  1:1359000/1‑101 (MQ=255)
                       ggATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGcc                                                                          >  1:938693/1‑101 (MQ=255)
                        gATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCt                                                                         >  1:2332314/1‑101 (MQ=255)
                           tgtgTCCTTCGCGCCGCAGCGGCCCCGTTTTTCCTGGGGCGAAC.CGCGGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAg                                                                      <  2:2688947/101‑1 (MQ=255)
                           tgtgTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTA.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAg                                                                      >  2:2728943/1‑101 (MQ=255)
                               tCCTCCGCGCCGCAGCAGCCCCGCTTTTCCTGGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGgcgc                                                                  <  2:2820895/101‑1 (MQ=255)
                                ccTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGcgcg                                                                 >  2:3163745/1‑101 (MQ=255)
                                 cTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCgcgg                                                                >  1:360826/1‑101 (MQ=255)
                                    cgcgCGGCAGCGGCCCCTTTTTTCCTTGGGCGAAC.CGCGGGGGGTTG.G.CGCGTCAGC.CAAGGGGGGGGGCGGCCCCCC.CCG.AAGCGCCTGAGGCGcggcgg                                                             <  1:611808/101‑1 (MQ=255)
                                       gccgcAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCt                                                          >  1:1961460/1‑101 (MQ=255)
                                       gccgcAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCt                                                          >  2:3399678/1‑101 (MQ=255)
                                       gccccAGCAGGCCCGCTTTTCCTTGGGCAAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCt                                                          <  2:2833210/101‑1 (MQ=38)
                                        ccggcgccggCCCCGTTTTCCTTGGGGTCAA.CCCCGGGGGGGT.GCCGCGCCGCCCCACGGGGGGGGC.CCCCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCt                                                          <  2:3530955/93‑1 (MQ=255)
                                        ccgcAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCtt                                                         >  1:209665/1‑101 (MQ=255)
                                         ccgcggcggCCCCGTTTTCCTTGGGGCAAA.CCGGGGGGGGTTGG.CGCGCCGGCAAAGGGGGGGGGCA.CCCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCtt                                                         <  2:2332146/94‑1 (MQ=255)
                                          gcagcaGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCgggggttc                                                       >  1:2935076/1‑96 (MQ=255)
                                           cagcagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTgcc                                                      >  2:2836743/1‑101 (MQ=255)
                                           cagcagGCCCGCTTTCCCTGGGGCGAAC.CGCCGGGGGTTG.G.CGCGTCAGCGAAAGGGGGGGGCAGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTgcc                                                      <  1:2527534/101‑1 (MQ=37)
                                              cagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                   >  2:2703489/1‑101 (MQ=255)
                                              cagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTgcccgc                                                   >  2:3528780/1‑101 (MQ=255)
                                              cagGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCCTggccgc                                                   >  2:3999064/1‑101 (MQ=38)
                                               agGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTgcccgcc                                                  >  1:2074361/1‑101 (MQ=255)
                                                ggCCCGCTTTTCATTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AACCGCCTGCGGCGCGGCGGGGTTcccccccc                                                 >  1:2296599/1‑101 (MQ=255)
                                                 gCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCa                                                >  1:1049247/1‑101 (MQ=255)
                                                    cttttttTCTTGGGGAAAA.CGGGGGGGGGTTGG.CGCG.CGGCGGAAGGGGGGGGCCGCCCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAAc                                              <  1:3742269/98‑1 (MQ=16)
                                                    cGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGGCCAACg                                             >  2:1126843/1‑101 (MQ=255)
                                                      cTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGaa                                           >  2:1894995/1‑101 (MQ=255)
                                                       ttttCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAg                                          >  2:2438132/1‑101 (MQ=255)
                                                       gtttCCTTGGGGGAAACCGCGGGGGGGTG.G.CGCGCCGGCGAAAGGGGGGGGCAGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGaa                                           <  1:712635/100‑1 (MQ=21)
                                                         ttCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTTCCCGCCCCACGAAggg                                        >  1:2238706/1‑100 (MQ=255)
                                                         ttCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAggg                                        >  2:191669/1‑100 (MQ=255)
                                                         ttCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCCTGCCCGCCCAACGAAggg                                        >  1:3163682/1‑100 (MQ=255)
                                                              tGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAac                                   >  1:2857549/1‑101 (MQ=255)
                                                              tGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAac                                   >  2:2501449/1‑101 (MQ=255)
                                                               gggCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                  >  2:2226295/1‑101 (MQ=255)
                                                               gggCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCCTGCCCGCCCAACGAAGGCGCAacc                                  >  1:3426237/1‑101 (MQ=255)
                                                                ggggAAA.CGGCGGGGGGGT.G.CGCCGCCCCCAGAGGGGGGGGCCGCCGCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAacc                                  <  2:3295713/98‑1 (MQ=12)
                                                                ggCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaccc                                 >  2:3384042/1‑100 (MQ=255)
                                                                 gCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAacccc                                >  1:1542790/1‑99 (MQ=25)
                                                                  cGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAaacacc                               >  1:4070473/1‑101 (MQ=255)
                                                                   gAAA.CGCGGGGGGGTG.G.CGCGTCAGCGAAAGCGGGGGGCCGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                              <  2:4066625/101‑1 (MQ=25)
                                                                   gAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCt                              >  2:3844775/1‑101 (MQ=255)
                                                                    aaa.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCGCCCCC.CC..AAGCGCCTGCGGCGCGGCGGGCTTTCCCGCCCCACGAACGCCCCACCACCtc                             >  2:3990590/1‑100 (MQ=255)
                                                                    aaa.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCtt                             <  2:2005426/101‑1 (MQ=255)
                                                                     aa.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCCACCACCTTc                            >  1:2973075/1‑101 (MQ=37)
                                                                          gcGGGGGGTTGG.GGGGCCAGCGAAGGGGGGGGGCGGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGt                         <  1:2584068/100‑1 (MQ=18)
                                                                          ccGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCCCCTTCAGTc                        >  2:1930683/1‑101 (MQ=255)
                                                                           cGGGGGGTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCg                       >  2:2767215/1‑101 (MQ=255)
                                                                            ggggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                      >  2:2359289/1‑101 (MQ=255)
                                                                            ggggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCgg                      >  1:623155/1‑101 (MQ=255)
                                                                             gggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCCTCCGTCGGc                     >  1:1467093/1‑101 (MQ=25)
                                                                             gggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGc                     >  2:2066494/1‑101 (MQ=255)
                                                                              ggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCCACGAAGGCGCAACCACCTTCAGTCGGCt                    >  1:954171/1‑101 (MQ=25)
                                                                              ggggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCt                    >  2:3059592/1‑101 (MQ=255)
                                                                              ggggTT.G.CGCCTCAGCGCAGGGGGGGGGCAGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCt                    <  2:2562354/101‑1 (MQ=255)
                                                                               gggTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCtt                   >  2:2507729/1‑101 (MQ=255)
                                                                                 gTT.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                 >  2:4116334/1‑101 (MQ=255)
                                                                                 gTT.G.CGCGCCGGCGCAAGGGGGGGGCACCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                 <  2:546626/101‑1 (MQ=25)
                                                                                  tt.G.CGCGTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCgc                >  2:1544437/1‑100 (MQ=255)
                                                                                  gtgg.cgcGGCGGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCg                 <  2:2646593/98‑1 (MQ=21)
                                                                                        gcgTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc            >  1:1865637/1‑101 (MQ=255)
                                                                                        gcgTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATccc            >  2:2416981/1‑101 (MQ=255)
                                                                                         cgTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATcccc           >  2:2303807/1‑101 (MQ=255)
                                                                                          gTCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca          >  2:3864902/1‑101 (MQ=255)
                                                                                           tCAGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac         >  2:161456/1‑101 (MQ=255)
                                                                                           gtacgcgaaGGGGGGGGCCGCCCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCcca          <  2:1845326/94‑1 (MQ=37)
                                                                                           gcAGCGCAGGGGGGGGGCGGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac         <  2:634169/100‑1 (MQ=25)
                                                                                            gggcgcAGGCGGGGGGCCGC.CCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacc        <  2:1333539/99‑1 (MQ=25)
                                                                                            ccggccAAGGGGGGGGCCGCCCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac         <  1:2712933/96‑1 (MQ=255)
                                                                                             gcgcgggggggggggCCCCCCCCCC.CCGAAAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccac         <  1:274570/94‑1 (MQ=25)
                                                                                             aGCGCAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCccacca       >  1:4155397/1‑101 (MQ=255)
                                                                                               cgcAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgc     >  2:1079998/1‑101 (MQ=255)
                                                                                                ggagggggggggCGCCCCCCCCGCGG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAg      <  2:944842/97‑1 (MQ=25)
                                                                                                 cAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc   >  2:1617568/1‑101 (MQ=255)
                                                                                                 cAAGCGGGGGGCAGCG.CCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAgcgc   >  1:1157509/1‑101 (MQ=255)
                                                                                                     |                                                                                                    
TCGCCCGGTGCGGTTTCCTGACAGGATTGTGTCCTTCGCGCCGCAGCAGGCCCGCTTTTCCTTGGGCGAAA.CGCCGGGGGGTT.G.CGCGTCAGCGCAAGGGGGGGGCAGCGCCCCC.CCG.AAGCGCCTGAGGCGCGGCGGGCTTGCCCGCCCAACGAAGGCGCAACCACCTTCAGTCGGCTTCGATCCCCACCAGCGCG  >  NC_014034/2559825‑2560021

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG