Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,579,493 A→C 100% S227A (TCC→GCC)  pgi ← glucose‑6‑phosphate isomerase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,579,4930A→C100.0% 633.4 171S227A (TCC→GCC) pgiglucose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  new base (95/76):  ref base (0/0):  total (95/76)

Only 100 of 183 total aligned reads displayed.

GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590
                                                                                                  |                                                                                                 
gACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGgcga                                                                                                 >  2:1684222/1‑101 (MQ=255)
   cGAATAGCCCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAAtt                                                                                              <  1:2088277/101‑1 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCTGTCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCAAATTg                                                                                             <  2:4193819/101‑1 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             >  2:3275713/1‑101 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             <  1:1598256/101‑1 (MQ=255)
    gAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTg                                                                                             <  1:2684001/101‑1 (MQ=255)
      aTAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTgcg                                                                                           >  1:1020563/1‑101 (MQ=255)
           gcccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCgccgcc                                                                                      <  1:178343/101‑1 (MQ=255)
            cccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGccgccg                                                                                     <  1:3741614/101‑1 (MQ=255)
             ccgcccTCCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCgg                                                                                    <  1:1078689/101‑1 (MQ=255)
             ccgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCgg                                                                                    >  2:2944292/1‑101 (MQ=255)
              cgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCggg                                                                                   >  1:1499473/1‑101 (MQ=255)
              cgcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATGGCGCCGCCggg                                                                                   >  1:3762286/1‑101 (MQ=255)
               gcccACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGt                                                                                  >  1:3083105/1‑101 (MQ=255)
                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGGGAATTACGCCGCCGGGTcc                                                                                >  2:2428435/1‑101 (MQ=255)
                 ccacccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTcc                                                                                <  1:2501178/101‑1 (MQ=255)
                    cccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTg                                                                             <  1:3880652/101‑1 (MQ=255)
                     ccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGa                                                                            <  1:2600005/101‑1 (MQ=255)
                     ccaATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGa                                                                            >  2:3414666/1‑101 (MQ=255)
                       aaTCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGAcc                                                                          <  1:4211743/101‑1 (MQ=255)
                          ccTGGAGGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTc                                                                       <  2:4036021/101‑1 (MQ=255)
                          ccTCGAAGCCGAAGACGNGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTc                                                                       <  2:1799371/101‑1 (MQ=255)
                          ccTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCCGGTCCGTGACCTTc                                                                       >  2:2853092/1‑101 (MQ=255)
                           cTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcg                                                                      >  1:2887754/1‑101 (MQ=255)
                             cGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgcc                                                                    >  1:1391836/1‑101 (MQ=255)
                              gAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccg                                                                   >  2:3942373/1‑101 (MQ=255)
                               aaGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccgc                                                                  <  2:2856272/101‑1 (MQ=255)
                               aaGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccgc                                                                  >  2:1201063/1‑101 (MQ=255)
                               aaGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTcgccgc                                                                  >  2:1918916/1‑101 (MQ=255)
                                  ccGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCAt                                                               >  1:681308/1‑101 (MQ=255)
                                   cGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATc                                                              >  1:557859/1‑101 (MQ=255)
                                   cGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGGGACCTTCGCCGCCATc                                                              >  2:555558/1‑101 (MQ=255)
                                    gAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATcc                                                             <  2:3305927/101‑1 (MQ=255)
                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            <  1:3126495/101‑1 (MQ=255)
                                     aaGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            >  2:604882/1‑101 (MQ=255)
                                     aaGACGCGCGCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCa                                                            <  2:4205930/101‑1 (MQ=255)
                                        aCGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgc                                                         >  2:668482/1‑101 (MQ=255)
                                        aCGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGgc                                                         <  1:1219365/101‑1 (MQ=255)
                                          gcgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGAGCTGCCGGGTCCGTGACCTTCGCCGCCATCCAGgcgc                                                       >  1:3614186/1‑101 (MQ=255)
                                           cgcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGcgcg                                                      >  1:2979353/1‑101 (MQ=255)
                                            gcTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgg                                                     <  1:1951305/101‑1 (MQ=255)
                                              tCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggc                                                   <  1:3455154/101‑1 (MQ=255)
                                              tCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggc                                                   >  2:3718073/1‑101 (MQ=255)
                                               cgggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcg                                                  >  2:2341160/1‑101 (MQ=255)
                                               cgggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcg                                                  >  1:1870178/1‑101 (MQ=255)
                                                gggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcgg                                                 <  2:3916413/101‑1 (MQ=255)
                                                 ggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGt                                                >  1:2019007/1‑101 (MQ=255)
                                                 ggcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGGCCGTGACCTTCGCCGCCCCCCAGGCgcggggcgg                                                >  1:1430812/1‑97 (MQ=255)
                                                  gcgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCgcgggcgggt                                               >  2:855494/1‑99 (MQ=255)
                                                   cgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                              <  1:1000631/101‑1 (MQ=255)
                                                   cgggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGttt                                              >  1:1653101/1‑101 (MQ=255)
                                                    gggATGCCGAAAGCCGCCGTCCTGTGGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTc                                             >  2:1580699/1‑101 (MQ=255)
                                                     ggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                            >  1:2312902/1‑101 (MQ=255)
                                                     ggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                            >  1:2933078/1‑101 (MQ=255)
                                                     ggATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCCCGGCGAATTGCGCCGCCGCGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCg                                            >  2:1363063/1‑101 (MQ=255)
                                                      gATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCgg                                           <  2:2920531/101‑1 (MQ=255)
                                                            gAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCTGCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCCTTgg                                     >  2:3587403/1‑101 (MQ=255)
                                                            gAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTgg                                     >  2:3973002/1‑101 (MQ=255)
                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    >  2:4014826/1‑101 (MQ=255)
                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    >  1:2839131/1‑101 (MQ=255)
                                                             aaaGCCGCCGTCCTGTCGGTCGCGGTCGAGACCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGt                                    <  1:1384974/101‑1 (MQ=255)
                                                                gccgccGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCAt                                 <  2:3613933/101‑1 (MQ=255)
                                                                gccgccGTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCAt                                 >  1:501105/1‑101 (MQ=255)
                                                                 ccgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATc                                >  1:3334905/1‑101 (MQ=255)
                                                                  cgccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCg                               <  1:2369980/101‑1 (MQ=255)
                                                                   gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGt                              >  1:881973/1‑101 (MQ=255)
                                                                   gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGt                              >  1:3011854/1‑101 (MQ=255)
                                                                   gccgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGt                              >  2:3176664/1‑101 (MQ=255)
                                                                     cgTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtct                            >  1:3527057/1‑101 (MQ=255)
                                                                      gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGtctc                           >  1:2227232/1‑101 (MQ=255)
                                                                      gTCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCCCGGGCGGTTTCGGCATTGGTCATCGtctc                           <  2:4101582/101‑1 (MQ=255)
                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          >  1:2784952/1‑101 (MQ=255)
                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          >  1:468358/1‑101 (MQ=255)
                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          <  1:3176903/101‑1 (MQ=255)
                                                                       tCCTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCg                          <  1:333216/101‑1 (MQ=255)
                                                                         cTGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGAt                        >  2:3241963/1‑101 (MQ=255)
                                                                          tGTCGGTCGCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATc                       >  2:764732/1‑101 (MQ=255)
                                                                           gtcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCg                      <  2:736430/101‑1 (MQ=255)
                                                                            tcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgt                     <  2:2651728/101‑1 (MQ=255)
                                                                            tcggtcgCGGTCGAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgg                     >  2:1867670/1‑100 (MQ=255)
                                                                              ggtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtgg                   >  1:1708167/1‑101 (MQ=255)
                                                                               gtcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggt                  >  2:2288212/1‑101 (MQ=255)
                                                                                tcgcggtcgAGATCGGGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggtg                 <  2:3129626/101‑1 (MQ=255)
                                                                                tcgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCgtggtg                 >  1:2768381/1‑101 (MQ=255)
                                                                                 cgcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGa                >  2:4219816/1‑101 (MQ=255)
                                                                                  gcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGaa               >  2:501051/1‑101 (MQ=255)
                                                                                  gcggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGaa               >  2:1822000/1‑101 (MQ=255)
                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              >  2:3437055/1‑101 (MQ=255)
                                                                                   cggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAg              <  1:1814955/101‑1 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             >  2:1462050/1‑101 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             >  2:1223867/1‑101 (MQ=255)
                                                                                    ggtcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAgg             >  1:302098/1‑101 (MQ=255)
                                                                                      tcgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTc           >  2:1738378/1‑101 (MQ=255)
                                                                                       cgAGATCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCt          >  2:3029423/1‑101 (MQ=255)
                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCtt         <  1:421891/101‑1 (MQ=255)
                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCtt         <  1:3298858/101‑1 (MQ=255)
                                                                                        gagaTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGGTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGGCtt         >  1:4097136/1‑101 (MQ=255)
                                                                                           aTCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGa      <  2:3334660/101‑1 (MQ=255)
                                                                                            tCGCGGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGAc     <  1:3343837/101‑1 (MQ=255)
                                                                                               cgGCGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGcc  >  2:2012557/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GACCGAATAGCGCCCGCCCACCCAATCCTCGAAGCCGAAGACGCGCTCGGGCGGGATGCCGAAAGCCGCCGTCCTGTCGGTCGCGGTCGAGATCGCGGAGAATTGCGCCGCCGGGTCCGTGACCTTCGCCGCCATCCAGGCGCGGGCGGTTTCGGCATTGGTCATCGTCTCGATCGTGGTGAAGGTCTTGGACGCC  >  NC_014034/2579395‑2579590

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG