BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,950,759	T→A	2.5%	intergenic (+102/‑58)	RCAP_rcc02761 → / → RCAP_rcc02762	glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,950,759	0	T→A	2.5%	8.8 ‑0.4 ‑0.3	243	intergenic (+102/‑58)	RCAP_rcc02761/RCAP_rcc02762	glutathione S‑transferase/4Fe‑4S ferredoxin, iron‑sulfur cluster binding protein
Reads supporting (aligned to +/- strand): new base (4/2): ref base (127/105): total (134/109)
Fisher's exact test strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.06e-01

Only 100 of 256 total aligned reads displayed.


GCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTG  >  NC_014034/2950659‑2950859


gcaagcCGGGGGCTGTGCCCCCGCACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGt                                                                                                       <  2:3446005/101‑1 (MQ=255)


  aagcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCa                                                                                                     <  1:2033353/101‑1 (MQ=255)


   agcCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAg                                                                                                    >  2:3318109/1‑101 (MQ=255)


    gctgggggCTGTGCCCCCGGTCCCCCGGGATATTTGAGGCAAGTTGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAgg                                                                                                   <  2:1003642/98‑1 (MQ=21)


     ccGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGc                                                                                                  >  2:382528/1‑101 (MQ=255)


      cGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCa                                                                                                 >  2:2574293/1‑101 (MQ=255)


       gggggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCaa                                                                                                >  2:3145653/1‑101 (MQ=255)


         gggCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.g                                                                                             <  2:2615240/101‑1 (MQ=255)


           gCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAa.gag                                                                                           >  1:2799137/1‑101 (MQ=255)


              ctGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAgggg                                                                                        <  1:1031780/101‑1 (MQ=255)


               cttcgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAgggggg                                                                                      >  2:2184501/10‑100 (MQ=25)


                gCCGCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGc                                                                                      <  2:2104001/101‑1 (MQ=255)


                  cgcctcaCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATCCTTTCACCTTGCCGCAAATATCCCGGGGGGTGAATTGGCCG.AAGGCCAA.GAggggggcag                                                                                   >  1:1622618/7‑97 (MQ=25)


                   tcgctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGcg                                                                                  >  2:199602/7‑100 (MQ=2)


                   cccGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCCAA.GAGGGGGCAGc                                                                                   >  2:3556989/1‑101 (MQ=255)


                     cGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTc                                                                                 <  2:457238/101‑1 (MQ=255)


                      ctccccccGGGATATTTGCGGCAAGATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCC.AAAGGCCAA.GAGGGGGCAGCGccc                                                                               >  2:503644/4‑101 (MQ=2)


                       gACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccc                                                                               >  2:3710208/1‑101 (MQ=255)


                        aCCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTcccc                                                                              <  2:615215/101‑1 (MQ=255)


                         cccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTccccc                                                                             >  1:2211765/1‑101 (MQ=255)


                          ccccGGGATATTTGAGGCATGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            <  1:212387/101‑1 (MQ=255)


                          ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            <  2:3052347/101‑1 (MQ=255)


                          ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            >  1:1306311/1‑101 (MQ=255)


                          ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            <  1:519135/101‑1 (MQ=255)


                          ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            >  2:3292257/1‑101 (MQ=255)


                          ccccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCt                                                                            >  2:1462289/1‑101 (MQ=255)


                           cccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTg                                                                           >  1:912589/1‑101 (MQ=255)


                            ccGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGc                                                                          >  2:4046296/1‑101 (MQ=255)


                              gggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGccc                                                                        <  2:2524138/101‑1 (MQ=255)


                               ggATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGcccc                                                                       >  1:1465297/1‑101 (MQ=255)


                                 atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCgg                                                                     >  1:810749/1‑101 (MQ=255)


                                 atatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCgg                                                                     >  2:1332001/1‑101 (MQ=255)


                                  tatTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGc                                                                    >  1:2866071/1‑101 (MQ=255)


                                   atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                   >  1:4154413/1‑101 (MQ=255)


                                   atTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCt                                                                   >  1:3592778/1‑101 (MQ=255)


                                    tttGAGGCAAGATGAACCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTg                                                                  <  2:3210433/101‑1 (MQ=255)


                                    tttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTg                                                                  >  2:1017287/1‑101 (MQ=255)


                                     ttGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTga                                                                 <  1:2691546/101‑1 (MQ=255)


                                      tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                >  2:275599/1‑101 (MQ=255)


                                      tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                >  2:1631351/1‑101 (MQ=255)


                                      tGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgag                                                                >  1:722272/1‑101 (MQ=255)


                                       gAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagg                                                               >  1:3516674/1‑101 (MQ=255)


                                         ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                             <  2:2055288/101‑1 (MQ=255)


                                         ggCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgaggga                                                             <  2:1104761/101‑1 (MQ=255)


                                          gCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTgagggag                                                            <  1:1783586/101‑1 (MQ=255)


                                            aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                          >  2:4080541/1‑101 (MQ=255)


                                            aaGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGc                                                          >  2:1549181/1‑101 (MQ=255)


                                                taaaaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGaa                                                      <  1:3318350/99‑1 (MQ=255)


                                                 gAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGGCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAAc                                                     <  1:2091647/101‑1 (MQ=255)


                                                   aaCACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTg                                                   <  1:1501285/101‑1 (MQ=255)


                                                       cGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGaaa                                               >  1:3954277/1‑101 (MQ=255)


                                                        gCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAc                                              <  1:874198/101‑1 (MQ=255)


                                                         ccctgtgcATCTGGCGGCAAATATCCC.GGGGGTGAATTTGCCGTGAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAAcc                                             <  1:2346201/94‑1 (MQ=37)


                                                           ccTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACGtt                                           <  1:2415347/101‑1 (MQ=255)


                                                            cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                          >  2:3753431/1‑101 (MQ=255)


                                                            cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                          <  2:2357416/101‑1 (MQ=255)


                                                            cTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTg                                          <  2:1915751/101‑1 (MQ=255)


                                                             tttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGa                                         >  1:3890576/1‑101 (MQ=255)


                                                              ttCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGaa                                        <  2:1410095/101‑1 (MQ=255)


                                                               tCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAg                                       >  1:1690282/1‑101 (MQ=255)


                                                                cATCTTGCCCCAAATATCCC.GGGGGGGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGa                                      >  1:367406/1‑101 (MQ=255)


                                                                  tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGCGGCGGAACTGGAAACCTTGAAGAcc                                    >  2:1689891/1‑101 (MQ=255)


                                                                  tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                    >  2:725626/1‑101 (MQ=255)


                                                                  tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                    >  2:2828993/1‑101 (MQ=255)


                                                                  tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                    >  2:2428497/1‑101 (MQ=255)


                                                                  tCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAcc                                    >  1:2779616/1‑101 (MQ=255)


                                                                   cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                   <  1:3002919/101‑1 (MQ=255)


                                                                   cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                   >  2:17597/1‑101 (MQ=255)


                                                                   cTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGAccc                                   >  1:2654984/1‑101 (MQ=255)


                                                                    ttGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                                  >  1:2011015/1‑101 (MQ=255)


                                                                    ttGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCg                                  >  1:2581123/1‑101 (MQ=255)


                                                                     tGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGc                                 <  1:1569848/101‑1 (MQ=255)


                                                                         ccAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGa                             <  2:640980/101‑1 (MQ=255)


                                                                          cAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGAt                            <  1:1806465/101‑1 (MQ=255)


                                                                           aaaTCTCCC.GGTGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           <  2:4126691/101‑1 (MQ=255)


                                                                           aaaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATc                           >  1:2532418/1‑101 (MQ=255)


                                                                            aaTATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCg                          <  1:22972/101‑1 (MQ=255)


                                                                               atCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACATTGAAGACCCGCCTGATCGAag                       <  1:1721307/101‑1 (MQ=255)


                                                                                tCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAaga                      <  2:1843744/101‑1 (MQ=255)


                                                                                 ccc.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAagag                     >  1:1083363/1‑101 (MQ=255)


                                                                                     gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                  <  1:313755/101‑1 (MQ=255)


                                                                                     gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                  >  1:3220293/1‑101 (MQ=255)


                                                                                     gggggTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGcc                  >  1:2569383/1‑101 (MQ=255)


                                                                                       ggttGATTTTGGCGGCAGGCAAA.AAGGGGGAACCTCACCCTGCCCCGTCTGAGGGAGGCGGAACTGGAATCCTTGAAGACCCGCCGGATCGAAGAGGCCcg                <  2:3658567/98‑1 (MQ=255)


                                                                                       ggggtaaTTTGCCGTCAGCCAAA.GAGGGGGCTGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAGCCTTGAAGACCCGCCTGATCGAAGAGGCCcg                <  1:3795648/96‑1 (MQ=255)


                                                                                       cttccgatctGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcg                <  2:2860018/92‑1 (MQ=255)


                                                                                         gTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcg              >  2:1784715/1‑101 (MQ=255)


                                                                                           gAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCcgcgcg            >  2:2681071/1‑101 (MQ=255)


                                                                                            aaTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGa           >  2:1168878/1‑101 (MQ=255)


                                                                                             aTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt          >  2:775426/1‑101 (MQ=255)


                                                                                             aTTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGAt          <  1:1753380/101‑1 (MQ=255)


                                                                                              tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         <  2:94837/101‑1 (MQ=255)


                                                                                              tttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         <  2:1306215/101‑1 (MQ=255)


                                                                                              tttGCCGGCAGGAAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATc         <  1:4081334/101‑1 (MQ=255)


                                                                                               ttGGCGGCAGGGAAAGGAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGACGCCCGCGCGATc         <  1:2293646/101‑1 (MQ=255)


                                                                                               ttGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCg        >  2:1209218/1‑101 (MQ=255)


                                                                                                 gCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGc      >  1:3447490/1‑101 (MQ=255)


                                                                                                   cGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGCAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCtt    >  2:2160348/1‑101 (MQ=255)


                                                                                                    gTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCttt   >  1:3192292/1‑101 (MQ=255)


                                                                                                     tCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTg  >  1:1348041/1‑101 (MQ=255)


GCAAGCCGGGGGCTCTGCCCCCGGACCCCCGGGATATTTGAGGCAAGATGAAACACGCCCCTTTCATCTTGCCCCAAATATCCC.GGGGGTGAATTTGCCGTCAGGCAAA.GAGGGGGCAGCTCCCCCTGCCCCGGCTGAGGGAGGCGGAACTGGAAACCTTGAAGACCCGCCTGATCGAAGAGGCCCGCGCGATCGGCTTTG  >  NC_014034/2950659‑2950859

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG