Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,982,999 A→G 100% V81A (GTG→GCG)  hslR ← heat shock protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,982,9990A→G100.0% 644.2 174V81A (GTG→GCG) hslRheat shock protein
Reads supporting (aligned to +/- strand):  new base (100/74):  ref base (0/0):  total (100/74)

Only 100 of 183 total aligned reads displayed.

GGCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCACGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGCC  >  NC_014034/2982901‑2983096
                                                                                                   |                                                                                                 
ggCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCg                                                                                                 >  2:2599887/1‑101 (MQ=255)
    cgggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCtt                                                                                             >  2:3960812/1‑101 (MQ=255)
    cgggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCtt                                                                                             >  1:610153/1‑101 (MQ=255)
    cgggcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCtt                                                                                             >  2:202427/1‑101 (MQ=255)
     tctcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTc                                                                                            <  2:1817556/98‑1 (MQ=255)
       gcgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCg                                                                                          <  1:743442/101‑1 (MQ=255)
        cgggGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCgg                                                                                         >  1:938358/1‑101 (MQ=255)
          gggAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGgcg                                                                                       >  1:1667063/1‑101 (MQ=255)
             aaaGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCgg                                                                                    >  1:588990/1‑101 (MQ=255)
               aGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCgggg                                                                                  >  2:2657544/1‑101 (MQ=255)
               aGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCgggg                                                                                  >  2:2228420/1‑101 (MQ=255)
               aGGCGATGCCTTTCCCGATCCGCCCGAAACCGGAGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGCCGCgggg                                                                                  >  2:2684720/1‑101 (MQ=255)
                 gCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGcc                                                                                <  2:652007/101‑1 (MQ=255)
                 gCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGcc                                                                                <  1:3059505/101‑1 (MQ=255)
                 gCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCCGCGCGGGGcc                                                                                >  2:1797939/1‑101 (MQ=255)
                  cGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCcg                                                                               >  1:319237/1‑101 (MQ=255)
                   gATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCcgc                                                                              >  2:1861520/1‑101 (MQ=255)
                      ggatttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCgcggc                                                                           >  1:4229151/4‑101 (MQ=255)
                       ccTTTCACGATCCGGCAGAAACCGGTGATGGCAACAGATCACTCCCCCCGCGCGACG.TCAGACGCGTAGAGCGTCGCGGCGGCCGGCGAGGGACCgcggcg                                                                          >  2:3768916/1‑101 (MQ=255)
                         tttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGcgcg                                                                        >  2:14011/1‑101 (MQ=255)
                         tttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGcgcg                                                                        >  1:1592860/1‑101 (MQ=255)
                         tttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGcgcg                                                                        >  2:893652/1‑101 (MQ=255)
                          ttCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGcgcgg                                                                       >  2:3706729/1‑100 (MQ=255)
                            cccGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGc                                                                     >  1:3938586/1‑101 (MQ=255)
                              cGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCg                                                                   >  1:1859427/1‑101 (MQ=255)
                              cGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGGGCCg                                                                   >  1:743140/1‑101 (MQ=255)
                                aTCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCa                                                                 <  2:2093694/101‑1 (MQ=255)
                                aTCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCa                                                                 >  2:664123/1‑101 (MQ=255)
                                 tCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAg                                                                <  2:2246854/101‑1 (MQ=255)
                                  ccGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGGGCCGCAgg                                                               >  2:1495572/1‑101 (MQ=255)
                                   cGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGc                                                              <  1:1025483/101‑1 (MQ=255)
                                   cGCCCGAAACCGGGGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGGCGGGCCGCGGCGCGGGCCGCCGGc                                                              >  2:1951076/1‑101 (MQ=255)
                                    gCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGcc                                                             >  1:255456/1‑101 (MQ=255)
                                    gCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGcc                                                             >  2:2864961/1‑101 (MQ=255)
                                     cccGAAACCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCa                                                            <  1:3458229/101‑1 (MQ=255)
                                     cccGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGGGCCGCAGGCCa                                                            >  2:3457977/1‑101 (MQ=255)
                                     cccGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCCCCGGGCGGGCCGCAGGCCa                                                            >  2:3277061/1‑101 (MQ=255)
                                       cGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAgg                                                          >  2:1554704/1‑101 (MQ=255)
                                       cGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAgg                                                          >  2:1794962/1‑101 (MQ=255)
                                       cGAAACCGGGGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAgg                                                          <  2:3198671/101‑1 (MQ=255)
                                        gAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGGCGCGGCGCGGGCCGCAGGTCAggg                                                         >  1:4168442/1‑100 (MQ=255)
                                        gAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGa                                                         <  1:2657750/101‑1 (MQ=255)
                                        gAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGa                                                         >  1:3791000/1‑101 (MQ=255)
                                        gAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGa                                                         >  1:2892616/1‑101 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCCGGCCAGGAt                                                        >  1:208816/1‑101 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGAt                                                        <  2:3030256/101‑1 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGGGCCGCAGGCCAGGaa                                                        >  1:1386405/1‑100 (MQ=255)
                                         aaaCCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGCGCCGCAGGCCAGGag                                                        >  2:908854/1‑100 (MQ=255)
                                           aCCGGTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgc                                                      <  1:637623/101‑1 (MQ=255)
                                            ccGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgcg                                                     >  1:1783518/1‑101 (MQ=255)
                                            ccGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgcg                                                     <  2:1941193/101‑1 (MQ=255)
                                             cGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGGTgcgc                                                    >  1:122661/1‑101 (MQ=255)
                                             cGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATgcgc                                                    <  1:2448720/101‑1 (MQ=255)
                                              ggTGATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgca                                                   <  1:2231613/101‑1 (MQ=255)
                                                tGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGCCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgcagc                                                 >  2:1422114/1‑101 (MQ=255)
                                                ggATGGCACCGGACCCCCCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgcagc                                                 <  2:803795/100‑1 (MQ=255)
                                                ggATGGCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgcagc                                                 <  2:599285/100‑1 (MQ=255)
                                                 gATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCgcagca                                                >  2:2970863/1‑101 (MQ=255)
                                                  aTGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCAc                                               >  2:3840681/1‑101 (MQ=255)
                                                    ggCAACGGATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCAcgc                                             <  1:4003551/101‑1 (MQ=255)
                                                       aaCGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACgcgcc                                          >  1:3539214/1‑100 (MQ=255)
                                                       aaCGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCa                                          >  2:1896410/1‑101 (MQ=255)
                                                           gATCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTc                                      <  1:202465/101‑1 (MQ=255)
                                                           gATCCCCCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTc                                      <  1:1554808/101‑1 (MQ=255)
                                                           gATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTc                                      >  1:473621/1‑101 (MQ=255)
                                                            aTCCCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCg                                     <  2:4228889/101‑1 (MQ=255)
                                                              cccccccccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgc                                   <  2:840533/97‑1 (MQ=255)
                                                              cACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgc                                   >  2:4183932/1‑101 (MQ=255)
                                                               ccTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgcg                                  <  1:1047832/100‑1 (MQ=255)
                                                               aCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGGCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCCCGgcg                                  >  1:1910768/1‑101 (MQ=255)
                                                               aCTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGgcg                                  >  1:2772431/1‑101 (MQ=255)
                                                                  cccccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGccc                               <  1:1365596/101‑1 (MQ=255)
                                                                   ccccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGGAGCGCAGCACGCGCACCTCGGCGCCCa                              >  2:543800/1‑101 (MQ=255)
                                                                   ccccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCa                              >  2:2935849/1‑101 (MQ=255)
                                                                    cccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAg                             >  1:3562664/1‑101 (MQ=255)
                                                                    cccGCCGCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAg                             <  1:113331/101‑1 (MQ=255)
                                                                     ccgccgCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGa                            <  1:1077164/101‑1 (MQ=255)
                                                                     ccgccgCGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCCCGGCGCCCCGa                            >  2:3692559/1‑101 (MQ=255)
                                                                        ccgcGTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGGCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAAcc                         >  1:1728681/1‑101 (MQ=255)
                                                                         cgcgTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCa                        <  2:1375468/101‑1 (MQ=255)
                                                                          gcgTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCCGGCCAGGCTGCGCCGCACGCGCACCTCGGCGCCCAGAACCAg                       >  1:4079778/1‑101 (MQ=255)
                                                                          gcgTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAg                       <  1:2003673/101‑1 (MQ=255)
                                                                           cgTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGc                      >  2:2529558/1‑101 (MQ=255)
                                                                            gTCGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCg                     >  2:4071277/1‑101 (MQ=255)
                                                                              cGATCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTc                   >  1:2424880/1‑101 (MQ=255)
                                                                                aTCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAg                 >  2:4101/1‑101 (MQ=255)
                                                                                aTCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAg                 >  1:3096066/1‑101 (MQ=255)
                                                                                aTCAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAg                 >  1:2706458/1‑101 (MQ=255)
                                                                                  cAG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCa               <  1:279052/101‑1 (MQ=255)
                                                                                   aG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCAc              >  1:2803876/1‑101 (MQ=255)
                                                                                   aG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCAc              <  1:161574/101‑1 (MQ=255)
                                                                                   aG.CGCGTAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCAc              <  1:2600078/101‑1 (MQ=255)
                                                                                          tAGAGCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGcc        <  2:122637/101‑1 (MQ=255)
                                                                                           agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       <  2:1395002/101‑1 (MQ=255)
                                                                                           agagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGccc       >  1:848608/1‑101 (MQ=255)
                                                                                            gagCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCg      <  1:3447264/101‑1 (MQ=255)
                                                                                              gCGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCggg    >  1:334615/1‑101 (MQ=255)
                                                                                               cGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCGCCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGc   <  2:2499429/101‑1 (MQ=255)
                                                                                               cGTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGc   <  2:3938326/101‑1 (MQ=255)
                                                                                                gTCGCGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGcc  <  2:1680464/101‑1 (MQ=255)
                                                                                                   |                                                                                                 
GGCACGGGCGGGGAAAGGCGATGCCTTTCCCGATCCGCCCGAAACCGGTGATGGCAACGGATCACTCCCCCGCCGCGTCGATCAG.CGCGTAGAGCGTCACGGCTTCCGGCGCGGGGCCGCGGCGCGTGCCGCAGGCCAGGATGCGCAGCACGCGCACCTCGGCGCCCAGAACCAGCGTCAGCACGTCGCCCGGGCC  >  NC_014034/2982901‑2983096

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG