Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,163 A→T 4.2% intergenic (‑146/+146) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1630A→T 4.2% 10.3 ‑2.5 ‑0.6144intergenic (‑146/+146)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (85/53):  total (91/53)
Fisher's exact test strand distribution p-value = 8.53e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.63e-01

Only 100 of 192 total aligned reads displayed.

ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGG  >  NC_014034/3068063‑3068262
                                                                                                       |                                                                                                      
cgatctgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCa                                                                                                        <  1:3147919/95‑1 (MQ=255)
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCa                                                                                                        <  2:2809970/101‑1 (MQ=255)
 ttCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAg                                                                                                       <  2:1059084/101‑1 (MQ=255)
  tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCCAGGGCCCAAA.GG.CCCCC.CGCCaag                                                                                                      >  2:492982/1‑99 (MQ=255)
    gcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAgcgc                                                                                                    <  1:3744611/101‑1 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGcgccc                                                                                                  <  2:3888733/101‑1 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGcgccc                                                                                                  >  2:3192958/1‑101 (MQ=255)
        aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGcgcccgc                                                                                                >  1:567069/1‑101 (MQ=255)
          ccGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCgcccgccc                                                                                              <  2:2020584/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGcccgccccg                                                                                            <  2:536416/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGcccgccccg                                                                                            >  2:3696025/1‑101 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGcccgccccg                                                                                            >  2:2665269/1‑101 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGcccgccccg                                                                                            <  1:371243/101‑1 (MQ=255)
                cggtGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCa                                                                                        <  2:1064825/98‑1 (MQ=255)
                cGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCa                                                                                        <  1:2671236/101‑1 (MQ=255)
                cGATGGGCAGGCGGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCa                                                                                        <  2:2030896/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                       <  1:1533616/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                       >  1:1475925/1‑101 (MQ=255)
                  aggggCGGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      <  1:1774654/99‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      <  2:1111439/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      <  1:1729808/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      >  1:1793946/1‑101 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      <  1:2300756/101‑1 (MQ=255)
                  aTGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATg                                                                                      <  1:1065946/101‑1 (MQ=255)
                    gggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc                                                                                    <  2:3984774/101‑1 (MQ=255)
                     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.g                                                                                  >  2:3773314/1‑101 (MQ=255)
                     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.g                                                                                  >  1:1920046/1‑101 (MQ=255)
                      gCAGGCTGACCGAGGCCATCGCCCAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.gg                                                                                 >  2:744042/1‑101 (MQ=255)
                       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.ggg                                                                                <  2:567005/101‑1 (MQ=255)
                       cAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.ggg                                                                                <  1:3509296/101‑1 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.gggc                                                                               >  2:1473617/1‑101 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.gggc                                                                               >  2:2784563/1‑101 (MQ=255)
                         ggCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATggc.ggggg                                                                              >  1:1984297/1‑99 (MQ=255)
                            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCtt                                                                           <  2:3120389/101‑1 (MQ=255)
                            tGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCACGGC.GGgggcgt                                                                           >  2:2022118/1‑99 (MQ=255)
                             gACCGAGGCGATCGCCGAGGTCATGCGGACCAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTtc                                                                          <  1:329548/101‑1 (MQ=255)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTtc                                                                          <  2:265709/101‑1 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGGCCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGGGCCCGCCCCGCCCATGGC.GGGCGCTtct                                                                         <  1:3999814/101‑1 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTtct                                                                         <  1:3868690/101‑1 (MQ=255)
                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTtctc                                                                        >  1:904195/1‑101 (MQ=255)
                                      cATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCCcgc.ccgcc                                                                >  1:4224809/1‑101 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.ccgccg                                                               >  2:1928512/1‑101 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.cccccg                                                               >  1:1336588/1‑101 (MQ=255)
                                       aTCGCCGAGGTCAGGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.ccgccg                                                               <  1:45123/101‑1 (MQ=255)
                                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCg.gg                                                            <  1:4009409/101‑1 (MQ=255)
                                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCCC.CCGCCc.gg                                                            >  2:2306011/1‑101 (MQ=255)
                                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCCTGGC.GGGCGCTTCTCGC.CCCCCg.ggg                                                           >  2:3830834/1‑101 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.gggcg                                                         <  2:3419522/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCCCCCATGGC.GGGCGCTTCGCCC.CCTGCg.ggggg                                                         >  1:758458/1‑99 (MQ=17)
                                             gAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.gggcgg                                                        <  1:2595777/101‑1 (MQ=255)
                                              aGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCCG.CCGCCC.gggcggg                                                       >  2:1599010/1‑101 (MQ=255)
                                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.Gggcgggcg                                                     <  2:2439155/101‑1 (MQ=255)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGGcc                                                   >  2:3126655/1‑101 (MQ=255)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGcc                                                   >  1:669641/1‑101 (MQ=255)
                                                   cggCGGACCGGTGCCCCCGGGCCCGCCG.GGGCCCCAA.GG.CCCCCGGGCCAGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCg                                                  <  1:1434531/99‑1 (MQ=11)
                                                   aTGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGGCCg                                                  >  1:2382047/1‑101 (MQ=255)
                                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGG.CCGCCG.GGGGGGGCGCCgga                                                >  2:1235606/1‑100 (MQ=255)
                                                      cgaaaCAGTGGTCCCATGACCCCCGAGTGCCCCAA.GG.CACTC.GGGCAGCGCCCGCCCCGCCCATGGC.GGGCGGTTCTCGC.CCCCCC.GGGGGGGGGGCGGcc                                               >  2:444392/4‑101 (MQ=17)
                                                      cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCCGcc                                               >  2:1378869/1‑101 (MQ=255)
                                                      cGGAACAGTGCCCTCGTGACCGCCGAGGGCCGAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGcc                                               <  1:3462105/101‑1 (MQ=255)
                                                          acgggggCCTCGGGCCCGCCGAGGCCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCT.GGGCGGGCGCCGGCCTctg                                           <  2:2195308/96‑1 (MQ=14)
                                                          acatggcccccGTGCCCGCGGAGGGCCAAAA.GG.CCCTC.GGCCAGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTctg                                           <  2:898105/91‑1 (MQ=11)
                                                          aCAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGGCCGGCCTctg                                           >  2:1095190/1‑101 (MQ=255)
                                                          aCAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCCCCG.GGGCGGGGGCCGGCCCctg                                           >  1:410385/1‑101 (MQ=255)
                                                          aCAATGGCCCCGTGACCGGCGAGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGC.GGGGGC.GGGCGCGCCGCCG.GGGCGGGGGGCgggggcct                                           >  1:1355827/1‑95 (MQ=17)
                                                             ggggCCTGGTGCCCGCCGGGGGCCCAAG.GC.CCCTC.GCCCGGCGCCCGCCCCGCCCAGGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTctgctg                                        <  2:2882145/99‑1 (MQ=14)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCtgctgc                                       >  2:251806/1‑101 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCtgctgc                                       >  1:4166988/1‑101 (MQ=255)
                                                                gCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTgcgc                                     >  1:1203977/1‑101 (MQ=255)
                                                                   cggggcccgccg.GGGCCCAAA.GGCCCCCC.GGCCGGCGCCCGCCCCGCCCATGGG.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTg                                  <  1:2192267/95‑1 (MQ=11)
                                                                    cgggcccgcggAGGCCCCAAG.GG.CCCTC.GGCCGGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTgg                                 <  2:4166726/96‑1 (MQ=14)
                                                                    cGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCCCCG.GGGGGGGGGCCGGCCTCTGCTGCGCCTgg                                 >  2:2699520/1‑101 (MQ=255)
                                                                     gTGACCGCCGGGGGCCCAAA.GG.CCCTC.GGCCTGCGCCCGCCCCGCCATCGGCGGGGGGCTTCGCG..CCGCCGCGGGCGGGGGGCGGCCGCCGCGgcgggtgg                                 >  2:3553242/1‑96 (MQ=2)
                                                                     gTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCC.GGGCGGGGGCCCGCCCCTGCTGCGCCCggg                                >  1:1102957/1‑101 (MQ=14)
                                                                      tGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCCCCG.GGGCGGGCGCCCGCCTCCGCTGCCCCCgggg                               >  2:3334407/1‑101 (MQ=14)
                                                                       gcccgccgAGGCCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGa                              <  2:2823097/99‑1 (MQ=255)
                                                                        cccgccg.GGGCCCAAA.GG.CCCCC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTTTCCC.CCCCCG.GGGCGGGCGCCGGGCGCTGGTCCGCCTgcgggcg                            >  2:4145598/2‑98 (MQ=11)
                                                                        cccccccggggCCCAAAGGG.CCCTC.GCCCAGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGa                              <  1:1167614/93‑1 (MQ=14)
                                                                         ccgcggGGGCCCCAAG.GC.CCCTG.GGCCCGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCCC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                            <  1:2903314/101‑1 (MQ=14)
                                                                         ccgccgGGGGCCCAAA.GG.CCCCC.GGCCAGGCCCCGCCCCGCCCATGGC.GGGCCCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                            <  1:2784782/101‑1 (MQ=14)
                                                                          ccgcgggggCCCAAA.GGCCCCCC.GCCCCGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGaaa                            <  1:2271384/95‑1 (MQ=14)
                                                                            ccgGGGCCCCAAG.GG.CCCTC.GGCCGGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCGG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACgg                         <  2:1582821/101‑1 (MQ=17)
                                                                             cgAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCCGCGCCCGGGGGAACggg                        >  1:1092423/1‑100 (MQ=255)
                                                                             cgAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATAGC.GGGAGCTTCTCGC.CCGCCC.GGGCGGGGGCCGGGCCCTGCCCCGCCAGGGGAAACGGt                        >  1:1255514/1‑101 (MQ=14)
                                                                              gAGGGCCCAAA.GG.CCCTC.GGCGAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGNCCCGCCTCTGCTGCGGCTGGGGGAACgggc                       >  1:2410580/1‑99 (MQ=14)
                                                                              gAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCCCTGCTGCGCCCGGGGGAACgggc                       >  1:2987262/1‑99 (MQ=255)
                                                                               gaggCCCAAA.GGCCCCTC.GGCCGGCCCCCGNCCCGCCCAGGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTc                       <  1:195563/99‑1 (MQ=17)
                                                                               gaggCCCAAA.GG.CCCCCGGGCCGGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTc                       <  2:2545566/99‑1 (MQ=17)
                                                                               aGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGGCt                      >  1:2505581/1‑101 (MQ=255)
                                                                                gggCCCCAA.GGCCCCCC.GGCCGGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCt                      <  1:2367967/101‑1 (MQ=17)
                                                                                 gccccAAA.GC.CCCTC.GGCCGGCCCCCCCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCCGCTGCGCCTGGGGAAACGGTCTTg                    <  1:3696279/99‑1 (MQ=18)
                                                                                      aaa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGGCCGGCCCCGGCTGCGCCTGGGGGAACGGGCTTGGCagc               >  1:983699/1‑99 (MQ=17)
                                                                                       aa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCg              >  2:1867920/1‑101 (MQ=255)
                                                                                       aa.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCCC.CCGCCC.GGGCGGGGGGCCGCCTCTGCTGCGGCTTGGGGAACGGGCTTGGCACCg              >  2:3871726/1‑101 (MQ=17)
                                                                                           g.CCCTC.GCCCAGCCCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGccc           <  2:2146985/101‑1 (MQ=255)
                                                                                                 c.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGGGCCGGCCCCCGCTGCGCCTGGGGGAACGGGCTTGGCACCGCCCGGGcc      >  1:2878136/1‑100 (MQ=18)
                                                                                                   ggCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCACCGCCCGGGCTc     >  1:2390801/1‑101 (MQ=255)
                                                                                                   ggCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTc     >  2:3260323/1‑101 (MQ=255)
                                                                                                     ccAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg   >  2:2418916/1‑101 (MQ=255)
                                                                                                     ccAGCGCCCCCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCGG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg   <  2:1355698/101‑1 (MQ=255)
                                                                                                       |                                                                                                      
ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAA.GG.CCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCGCTTCTCGC.CCGCCG.GGGCGGGCGCCGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGG  >  NC_014034/3068063‑3068262

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 22 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG