Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	33.3	9	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (3/6):  ref base (0/0):  total (3/6)

CGCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGG  >  NC_014034/3223837‑3223953                                                                                                   |                   cgcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTTCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgg                  <  2:3918281/101‑1 (MQ=255) cgcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgg                  >  2:451569/1‑101 (MQ=255)  gcgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggg                 >  2:3649782/1‑101 (MQ=255)   cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  1:2266308/101‑1 (MQ=255)   cgcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggg                <  2:3734459/101‑1 (MQ=255)    gcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggggg               >  2:641373/1‑101 (MQ=255)    gcCGTCCCAGAACCCCGCCGCATGCACGGACCGAACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggggg               <  1:3650035/101‑1 (MQ=255)     ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggggg              >  1:1523648/1‑100 (MQ=255)        tCCCAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc           <  1:1458240/101‑1 (MQ=255)          ccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa         <  2:2007651/101‑3 (MQ=255)           cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggggggccaag        >  1:1217288/1‑94 (MQ=255)           cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGNGGGGCGGGGgcgccaag        >  2:88114/1‑98 (MQ=255)           cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag        <  2:2418801/101‑4 (MQ=255)              acccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgc     <  2:456889/99‑7 (MQ=255)               aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc    >  1:2096755/1‑94 (MQ=255)                ccccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcca   <  2:1943254/101‑9 (MQ=255)                 cccGCCGCATGCACGGACCGGACGGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag  >  1:3405439/1‑92 (MQ=255)                 cccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag  <  2:1678375/101‑10 (MQ=255)                                                                                                   |                   CGCGCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGG  >  NC_014034/3223837‑3223953 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG