Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,569 A→C 2.4% S1680A (TCG→GCG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5690A→C 2.4% 2.7 0.0 ‑0.6165S1680A (TCG→GCG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (2/2):  ref base (79/82):  total (81/84)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.54e-01

Only 100 of 171 total aligned reads displayed.

GTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAG  >  NC_014034/678469‑678668
                                                                                                    |                                                                                                   
 tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                    >  1:3831895/1‑101 (MQ=255)
 tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                    <  2:3985202/101‑1 (MQ=255)
 tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                    <  1:313592/101‑1 (MQ=255)
  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                                                                   >  2:3200483/1‑101 (MQ=255)
  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGCTGACCGCCGCGCTCGACa                                                                                                   >  2:1659322/1‑101 (MQ=255)
    tCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgc                                                                                                 >  1:1991933/1‑101 (MQ=255)
      aGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAgcgc                                                                                               >  1:3002062/1‑101 (MQ=255)
        ggcCGGAACGCCCGCGCTGCCCAGCGCCGCGATCTGCGCTGTGCCAAAGGCCGGCAGCTGCCCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                             <  2:3080606/100‑1 (MQ=255)
        cgcGGGAATGACCGCGCTGCCCAGCGCCGCGATTTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                             <  1:3602234/101‑1 (MQ=255)
         gcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccgc                                                                                            <  1:1102893/101‑1 (MQ=255)
         gcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccgc                                                                                            >  2:2137799/1‑101 (MQ=255)
          ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                                                                           >  2:3424816/1‑101 (MQ=255)
          ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                                                                           <  1:3585777/101‑1 (MQ=255)
           cGGAATGACCGCGCCGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGa                                                                                          >  2:1365768/1‑101 (MQ=255)
             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                                        >  1:567460/1‑101 (MQ=255)
              aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                                                                       <  2:658935/101‑1 (MQ=255)
               aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                      >  1:3114768/1‑101 (MQ=255)
                  aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                                   >  1:1263303/1‑101 (MQ=255)
                    cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                 <  1:3734388/101‑1 (MQ=255)
                    cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                 >  1:397335/1‑101 (MQ=255)
                    cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                 >  2:516496/1‑101 (MQ=255)
                    cgcgCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCg                                                                                 >  1:2251197/1‑101 (MQ=255)
                       gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                                              >  1:1986721/1‑101 (MQ=255)
                        cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                                             <  2:3614082/101‑1 (MQ=255)
                         tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGGGCTc                                                                            >  2:1120618/1‑101 (MQ=255)
                          gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                           >  2:475256/1‑101 (MQ=255)
                          gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                           <  1:3534065/101‑1 (MQ=255)
                          gCCCACCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                           <  1:1307823/101‑1 (MQ=255)
                           gcaaccgccgcGATCTGCGCCGCGCTCAGCGCGTCGAGCTGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAg                                                                          <  1:3621716/96‑1 (MQ=2)
                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTTCGCCGTGCTCAgc                                                                         >  2:2174497/1‑101 (MQ=255)
                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                         >  2:787833/1‑101 (MQ=255)
                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                         <  2:4008200/101‑1 (MQ=255)
                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                         <  1:1689131/101‑1 (MQ=255)
                              aGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACGGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                                       <  1:2283384/101‑1 (MQ=255)
                                cgccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccg                                                                     >  1:1636551/1‑101 (MQ=255)
                                 gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                    >  2:2682631/1‑101 (MQ=255)
                                   cgctATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGa                                                                  <  2:4165335/101‑1 (MQ=255)
                                   cgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                  >  2:2144532/1‑93 (MQ=2)
                                    gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                                                                 >  1:72198/1‑92 (MQ=2)
                                     cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                                >  1:3068068/1‑101 (MQ=255)
                                     cgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACt                                                                >  2:3328231/1‑101 (MQ=255)
                                      gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGCGCTCAGCGCCGCGACtt                                                               >  2:3802485/1‑101 (MQ=255)
                                       aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGNGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                              >  1:969489/1‑101 (MQ=255)
                                       aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                              >  1:4214905/1‑101 (MQ=255)
                                       aTCTGCCCCGGGCCAAAGGCCGCCAGCTGCGCGGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                              <  1:1762744/101‑1 (MQ=255)
                                        tCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGc                                                             >  2:2554255/1‑101 (MQ=255)
                                          tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                           >  2:2271508/1‑101 (MQ=255)
                                          tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                           <  2:3001837/101‑1 (MQ=255)
                                          ggcgcCGGGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                           <  1:1350871/100‑1 (MQ=255)
                                           gcgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCATCGCCGCGACTTGCcgc                                                          >  2:2385093/1‑101 (MQ=255)
                                             gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt                                                        >  1:435824/1‑101 (MQ=255)
                                               cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                      >  2:3156651/1‑101 (MQ=255)
                                               cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                      >  1:234227/1‑101 (MQ=255)
                                                 ggCCAACGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGCCAGCGCGGCGAGCGGCGCCGTGCTCAGCGCCGCGGCTTGCCGCgtcgtc                                                    <  2:397284/100‑1 (MQ=37)
                                                   ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCAGCGTCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                                  >  1:3620357/1‑101 (MQ=255)
                                                    cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                                 >  2:1435199/1‑101 (MQ=255)
                                                      aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                               <  1:3200722/101‑1 (MQ=255)
                                                       aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                              >  2:827949/1‑101 (MQ=255)
                                                       aGGCCGCCAGCTGCGCCGAGACGAGCGCGGTGACCGCCGCGCTCGAAAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                              <  1:1959498/101‑1 (MQ=255)
                                                        ggCCGCGAGCCGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAATTGCCGCGTCGTCAGCCCCg                                             <  2:1991807/101‑1 (MQ=255)
                                                        ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                             <  1:310574/101‑1 (MQ=255)
                                                        ggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCg                                             <  2:1244031/101‑1 (MQ=255)
                                                          ccgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCa                                           <  1:4096482/101‑1 (MQ=255)
                                                           cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                                          <  1:3775243/101‑1 (MQ=255)
                                                            gccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAAt                                         <  1:257497/101‑1 (MQ=255)
                                                              cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                       <  1:2937292/101‑1 (MQ=255)
                                                               aGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgc                                      >  2:2490774/1‑101 (MQ=255)
                                                                  tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                                   >  2:1950936/1‑101 (MQ=255)
                                                                  tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                                   <  2:3881475/101‑1 (MQ=255)
                                                                  tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                                   <  2:2723495/101‑1 (MQ=255)
                                                                   gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                                  <  2:932003/101‑1 (MQ=255)
                                                                   gcgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCcgcg                                  >  2:980818/1‑101 (MQ=255)
                                                                     gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAGTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                                >  1:3651573/1‑101 (MQ=255)
                                                                     gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                                <  1:4008623/101‑1 (MQ=255)
                                                                      ccgagccgagCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTg                               >  1:602925/1‑101 (MQ=255)
                                                                       cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                              >  2:1689342/1‑101 (MQ=255)
                                                                       cgagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTgg                              >  1:1520724/1‑101 (MQ=255)
                                                                        gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGt                             >  2:3022026/1‑101 (MQ=255)
                                                                        gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGt                             <  1:2584563/101‑1 (MQ=255)
                                                                        gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGt                             <  1:520590/101‑1 (MQ=255)
                                                                          gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                           <  2:2123320/101‑1 (MQ=255)
                                                                          gccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCa                           >  2:178510/1‑101 (MQ=255)
                                                                            cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGGGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                         >  1:3502953/1‑101 (MQ=255)
                                                                             gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                        <  1:2795922/101‑1 (MQ=255)
                                                                             gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                        <  2:1974657/101‑1 (MQ=255)
                                                                             gagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGcc                        >  2:2347774/1‑101 (MQ=255)
                                                                               gcgcGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgc                      >  1:2973627/1‑101 (MQ=255)
                                                                                cgcgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg                     >  2:1281538/1‑101 (MQ=255)
                                                                                  cgGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg                   <  1:2627346/101‑1 (MQ=255)
                                                                                    gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGAt                 <  1:316046/101‑1 (MQ=255)
                                                                                        ccgccgCGCTCGACAGCGCCGCGAACTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgc             >  2:2390702/1‑101 (MQ=255)
                                                                                          gccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgcgc           >  2:4206942/1‑101 (MQ=255)
                                                                                              cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCGGCGCTGGTCAGCCGCGCGATCTGCGCCGTg       <  2:2251038/101‑1 (MQ=255)
                                                                                              cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg       <  1:3341778/101‑1 (MQ=255)
                                                                                               gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg      <  2:621904/101‑1 (MQ=255)
                                                                                               gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg      <  2:2819962/101‑1 (MQ=255)
                                                                                               gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg      <  1:1075262/101‑1 (MQ=255)
                                                                                                  cGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCa   <  2:2886206/101‑1 (MQ=255)
                                                                                                   gACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAg  <  2:57809/101‑1 (MQ=255)
                                                                                                   gACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAg  >  1:216111/1‑101 (MQ=255)
                                                                                                    |                                                                                                   
GTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGGTCAG  >  NC_014034/678469‑678668

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG