Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,672 G→C 13.5% G645G (GGC→GGG RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6720G→C13.5% 58.1 ‑2.6 ‑0.3178G645G (GGC→GGGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (15/9):  ref base (64/90):  total (79/99)
Fisher's exact test strand distribution p-value = 7.62e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.93e-01

Only 100 of 194 total aligned reads displayed.

GCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAA  >  NC_014034/681572‑681772
                                                                                                    |                                                                                                    
  gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                                    >  2:2593980/1‑101 (MQ=255)
  gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                                    >  1:4116315/1‑101 (MQ=255)
  gccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGcc                                                                                                    <  1:2858596/101‑1 (MQ=255)
   ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                                   <  2:971818/101‑1 (MQ=255)
   ccgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCg                                                                                                   <  1:901015/101‑1 (MQ=255)
    cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGa                                                                                                  >  2:2274615/1‑101 (MQ=255)
    cgccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGCCTGGAGAGCGCGCCGa                                                                                                  >  2:2036020/1‑101 (MQ=255)
     gccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc                                                                                                 >  1:1006707/1‑101 (MQ=255)
     gccACCGTCGCCGAAGCCAGCGCCGAGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAc                                                                                                 >  2:1578068/1‑101 (MQ=255)
      ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCCCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc                                                                                                <  1:4196511/101‑1 (MQ=255)
      ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGAcc                                                                                                <  2:1354656/101‑1 (MQ=255)
      ccACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCCCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGCcc                                                                                                >  1:747687/1‑101 (MQ=255)
       cACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCt                                                                                               >  2:2921514/1‑101 (MQ=255)
         ccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGa                                                                                             <  1:3894161/101‑1 (MQ=255)
         ccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGa                                                                                             <  2:1057816/101‑1 (MQ=255)
         ccGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCAGa                                                                                             >  2:190420/1‑101 (MQ=255)
           gTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATc                                                                                           <  2:2136168/101‑1 (MQ=255)
             cGCCGAAGCCAGCGCCACGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCgg                                                                                         >  2:2359092/1‑101 (MQ=255)
              gCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGt                                                                                        <  2:3597446/101‑1 (MQ=255)
               gggtggaaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTg                                                                                       <  1:1115268/91‑1 (MQ=0)
               ccGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCCCAAAGCTGGAGAGCGAGCCGACCTGATCGGTg                                                                                       >  1:705985/1‑101 (MQ=255)
                cGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGc                                                                                      <  1:2889695/101‑1 (MQ=255)
                 gtggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGcc                                                                                     >  1:3398693/9‑100 (MQ=2)
                 gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                     >  2:3536127/1‑101 (MQ=255)
                 gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                     >  2:3212533/1‑101 (MQ=255)
                  tggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGccc                                                                                    >  1:1375244/8‑99 (MQ=2)
                  tggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGccc                                                                                    >  2:2847620/8‑99 (MQ=2)
                  aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc                                                                                    >  1:2446413/1‑101 (MQ=255)
                   ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                   <  1:443267/95‑1 (MQ=2)
                   ggcaatcgccgcGATCTGCCCCGCGCTCAGCCCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                   <  1:3213456/95‑1 (MQ=2)
                    gCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                                  >  1:1321171/1‑101 (MQ=255)
                    gCCAGCGCCGCGATCTGCGCCGTGCTCAGCCCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTTAg                                                                                  <  1:239802/101‑1 (MQ=255)
                     caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                 >  1:2582136/5‑100 (MQ=2)
                     caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                 >  2:4229225/5‑100 (MQ=2)
                     caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                 >  1:1901590/5‑100 (MQ=2)
                      cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                                <  2:3189532/101‑1 (MQ=255)
                      aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                                >  1:3433027/4‑99 (MQ=2)
                      aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                                >  2:4120183/4‑99 (MQ=2)
                        gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                              <  2:58803/101‑1 (MQ=255)
                         cgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGACCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                             >  1:3610598/1‑96 (MQ=2)
                         ccccgcGATCTGCGCCGCGCTCGGCGCCGCGATCGGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                             <  2:2267179/99‑6 (MQ=2)
                          gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                            <  2:1599639/101‑1 (MQ=255)
                            cgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                          <  2:3905978/101‑9 (MQ=2)
                            cgcgATCTGCGCCGCGCGCAGCGCCGCGATCTGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                          <  2:3477803/101‑9 (MQ=2)
                              cgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                        >  1:3185735/1‑101 (MQ=255)
                               gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                       <  2:2148809/101‑1 (MQ=255)
                                aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                      <  2:4090517/101‑1 (MQ=255)
                                aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                      >  2:1806225/1‑101 (MQ=255)
                                aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGACCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                      <  1:2626221/101‑1 (MQ=255)
                                 tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                     >  2:1963554/1‑101 (MQ=255)
                                 tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                     <  1:2501625/101‑1 (MQ=255)
                                   tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                                   >  1:858931/1‑101 (MQ=255)
                                   tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                                   >  1:818012/1‑101 (MQ=255)
                                          tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                            <  2:2446379/101‑1 (MQ=255)
                                          tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                            <  2:847004/101‑1 (MQ=255)
                                            cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                          >  1:3925254/1‑101 (MQ=255)
                                            cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                          <  1:182599/101‑1 (MQ=255)
                                             tCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                         >  2:2096260/1‑101 (MQ=255)
                                              cAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGc                                                        >  1:503272/1‑101 (MQ=255)
                                               aGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGcc                                                       >  1:4146552/1‑101 (MQ=255)
                                                gcgcCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGccc                                                      <  2:2750420/101‑1 (MQ=255)
                                                gcgcCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGccc                                                      <  1:1069634/101‑1 (MQ=255)
                                                 cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                                     <  1:3332894/101‑1 (MQ=255)
                                                 cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                                     >  1:2997330/1‑101 (MQ=255)
                                                  gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                                    <  2:2998060/101‑1 (MQ=255)
                                                    cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                                  <  1:1414177/101‑1 (MQ=255)
                                                     gccgccTCCACCGAGCCAAGCCCGCCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                                 <  2:552308/101‑1 (MQ=255)
                                                      ccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                                <  1:2149336/101‑1 (MQ=255)
                                                      ccgccGGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                                <  1:1258183/101‑1 (MQ=255)
                                                        gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                              >  1:2003087/1‑101 (MQ=255)
                                                         ccTGCCCCGAGCCAATCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgcc                                             <  2:4069730/101‑1 (MQ=255)
                                                          cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                            <  1:2418572/101‑1 (MQ=255)
                                                          cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                            >  1:2466505/1‑101 (MQ=255)
                                                             cACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCgcgc                                         >  2:468369/1‑101 (MQ=255)
                                                             cACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTCATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCgcgc                                         >  2:333747/1‑101 (MQ=255)
                                                                   gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGcccc                                   >  2:3062738/1‑100 (MQ=255)
                                                                   gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                   <  1:1568615/101‑1 (MQ=255)
                                                                   gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                   <  2:2885003/101‑1 (MQ=255)
                                                                    ccAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAg                                  <  2:817931/101‑1 (MQ=255)
                                                                     cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                                 >  2:1081535/1‑101 (MQ=255)
                                                                       aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                               <  1:2793657/101‑1 (MQ=255)
                                                                        gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                              <  1:2200595/101‑1 (MQ=255)
                                                                        gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                              <  2:1006616/101‑1 (MQ=255)
                                                                              ccACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATc                        <  2:264786/101‑1 (MQ=255)
                                                                                aCCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTg                      <  1:2359268/101‑1 (MQ=255)
                                                                                    cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc                  >  1:760534/1‑101 (MQ=255)
                                                                                     aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                 >  2:2492340/1‑101 (MQ=255)
                                                                                     aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                 >  2:1401504/1‑101 (MQ=255)
                                                                                     aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                 >  1:2608452/1‑101 (MQ=255)
                                                                                      aGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGt                >  2:2687837/1‑101 (MQ=255)
                                                                                          tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc            <  1:2528886/101‑1 (MQ=255)
                                                                                           ggAGAGCGCGCCGACCTGATTGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa           <  2:129664/101‑1 (MQ=255)
                                                                                           ggAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa           <  2:156219/101‑1 (MQ=255)
                                                                                            gagaGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAACCCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAg          <  1:2351777/101‑1 (MQ=255)
                                                                                              gagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCg        <  2:503215/101‑1 (MQ=255)
                                                                                                gcgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTc      <  1:3536380/101‑1 (MQ=255)
                                                                                                 cgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCg     <  1:2705751/101‑1 (MQ=255)
                                                                                                 cgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCg     <  2:75797/101‑1 (MQ=255)
                                                                                                  gcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGc    >  1:3674397/1‑101 (MQ=255)
                                                                                                  gcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGc    >  2:3722408/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GCGCCGCCACCGTCGCCGAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAA  >  NC_014034/681572‑681772

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG