Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,689 T→C 10.7% S640G (AGC→GGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6890T→C10.7% 40.3 ‑1.5 ‑0.0178S640G (AGC→GGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (11/8):  ref base (65/94):  total (76/102)
Fisher's exact test strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.82e-01

Only 100 of 189 total aligned reads displayed.

GAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAGC  >  NC_014034/681589‑681789
                                                                                                    |                                                                                                    
gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                                      >  2:3212533/1‑101 (MQ=255)
gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                                      >  2:3536127/1‑101 (MQ=255)
 aaGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTc                                                                                                     >  1:2446413/1‑101 (MQ=255)
  ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                                    <  1:2510584/95‑1 (MQ=2)
  ggcaatcgccgcGATCTGCCCCGCGCTCAGCCCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                                    <  1:3213456/95‑1 (MQ=2)
  aGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCa                                                                                                    <  1:3006872/101‑1 (MQ=255)
  aGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCa                                                                                                    <  2:357191/101‑1 (MQ=255)
   gCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                                                   >  1:1321171/1‑101 (MQ=255)
   gCCAGCGCCGCGATCTGCGCCGTGCGCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAg                                                                                                   <  2:1987696/101‑1 (MQ=255)
    caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                                  >  1:2582136/5‑100 (MQ=2)
    caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                                  >  1:858780/5‑100 (MQ=2)
    caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                                  >  2:4229225/5‑100 (MQ=2)
     cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                                                 <  2:3189532/101‑1 (MQ=255)
     aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                                                 >  2:4120183/4‑99 (MQ=2)
        ccccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgg                                                                                              <  1:3413877/99‑6 (MQ=2)
         gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                                             <  2:1599639/101‑1 (MQ=255)
         gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                                             >  2:2528702/1‑101 (MQ=255)
           cgcgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGa                                                                                           >  1:74805/1‑101 (MQ=255)
           cgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                                           <  2:3905978/101‑9 (MQ=2)
           cgcgATCTGCGCCGCGCGCAGCGCCGCGATCTGCGCGGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                                           <  2:3477803/101‑9 (MQ=2)
             cgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                                         >  1:3185735/1‑101 (MQ=255)
              gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                                        <  2:92767/101‑1 (MQ=255)
               aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                                       >  2:1806225/1‑101 (MQ=255)
               aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                                       >  1:2358092/1‑101 (MQ=255)
               aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                                       <  2:4090517/101‑1 (MQ=255)
               aTCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGACCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTg                                                                                       <  1:2626221/101‑1 (MQ=255)
                tCTGCGCGGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                                      <  2:541500/101‑1 (MQ=255)
                  tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                                                    >  1:858931/1‑101 (MQ=255)
                         tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                                             >  1:643976/1‑101 (MQ=255)
                         tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                                             <  2:2446379/101‑1 (MQ=255)
                         tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                                             <  2:495916/101‑1 (MQ=255)
                         tGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTc                                                                             <  2:847004/101‑1 (MQ=255)
                          gCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCa                                                                            >  2:3122903/1‑101 (MQ=255)
                           cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                                           <  1:182599/101‑1 (MQ=255)
                           cTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAg                                                                           >  1:3925254/1‑101 (MQ=255)
                            tCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAgc                                                                          >  2:2651853/1‑100 (MQ=0)
                            tCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAgc                                                                          >  1:142708/1‑100 (MQ=0)
                            tCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                                          >  2:2096260/1‑101 (MQ=255)
                              aGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGcc                                                                        <  2:3018397/101‑1 (MQ=255)
                               gcgcCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGccc                                                                       <  1:1069634/101‑1 (MQ=255)
                                cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                                                      <  1:3332894/101‑1 (MQ=255)
                                 gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                                                     <  1:2910806/101‑1 (MQ=255)
                                     ccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                                                 >  2:2351609/1‑101 (MQ=255)
                                     ccgccGGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAcc                                                                 <  1:1258183/101‑1 (MQ=255)
                                       gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                                               >  1:2003087/1‑101 (MQ=255)
                                       gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                                               >  1:81650/1‑101 (MQ=255)
                                       gccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgc                                                               >  2:1596598/1‑101 (MQ=255)
                                        ccTGCCCCGAGCCAATCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgcc                                                              <  2:4069730/101‑1 (MQ=255)
                                         cTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccgccg                                                             >  1:2466505/1‑101 (MQ=255)
                                          tGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACcgccgc                                                            <  2:2143761/101‑1 (MQ=255)
                                           gCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCcgcg                                                           >  1:4141520/1‑101 (MQ=255)
                                            cACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTCATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCgcgc                                                          >  2:333747/1‑101 (MQ=255)
                                             cccGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCt                                                         <  1:241396/100‑1 (MQ=255)
                                               cGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGc                                                       >  1:235959/1‑101 (MQ=255)
                                                  gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                                    <  2:2885003/101‑1 (MQ=255)
                                                  gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                                    <  2:2228663/101‑1 (MQ=255)
                                                  gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                                    <  1:895307/101‑1 (MQ=255)
                                                    cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                                                  >  2:1081535/1‑101 (MQ=255)
                                                    cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCCCGCAGCCCCGc                                                  >  1:4153894/1‑101 (MQ=255)
                                                     aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                                                 <  2:2473775/101‑1 (MQ=255)
                                                      aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                                                <  1:2793657/101‑1 (MQ=255)
                                                       gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                                               <  1:2200595/101‑1 (MQ=255)
                                                       gCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcccc                                               <  2:1006616/101‑1 (MQ=255)
                                                          cGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgcg                                            >  1:1766914/1‑101 (MQ=255)
                                                                    aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                                  >  2:1401504/1‑101 (MQ=255)
                                                                    aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                                  >  1:2608452/1‑101 (MQ=255)
                                                                    aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                                  <  1:2183158/101‑1 (MQ=255)
                                                                     aGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGt                                 >  2:2687837/1‑101 (MQ=255)
                                                                       gCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGc                               >  2:2796502/1‑101 (MQ=255)
                                                                        cTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGcc                              >  2:3841968/1‑101 (MQ=255)
                                                                         tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc                             <  1:2528886/101‑1 (MQ=255)
                                                                          ggAGAGCGCGCCGACCTGATTGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa                            <  2:129664/101‑1 (MQ=255)
                                                                          ggAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGcccc                            >  2:463206/1‑100 (MQ=255)
                                                                          ggAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa                            <  2:156219/101‑1 (MQ=255)
                                                                           gagaGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAg                           <  2:56056/101‑1 (MQ=255)
                                                                            agagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGc                          >  1:233060/1‑101 (MQ=255)
                                                                              agCGCGCCGACCTGATCGGTGCTCAGCTTGCCGGTCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGt                        <  1:3649116/101‑1 (MQ=255)
                                                                               gcgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTc                       <  1:3536380/101‑1 (MQ=255)
                                                                                ggcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCg                      <  2:1909188/100‑1 (MQ=255)
                                                                                cgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCg                      <  1:2705751/101‑1 (MQ=255)
                                                                                 gcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGc                     >  1:2862818/1‑101 (MQ=255)
                                                                                   gcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCaa                   >  2:80713/1‑101 (MQ=255)
                                                                                     cGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCACGc                 >  2:3988027/1‑101 (MQ=255)
                                                                                      gACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCt                <  2:2997108/101‑1 (MQ=255)
                                                                                       aCCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTg               >  2:3617116/1‑101 (MQ=255)
                                                                                       aCCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTg               <  1:3755672/101‑1 (MQ=255)
                                                                                       aCCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTg               >  2:331298/1‑101 (MQ=255)
                                                                                        ccTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTgc              <  2:74790/101‑1 (MQ=255)
                                                                                         cTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTgcg             <  1:480347/101‑1 (MQ=255)
                                                                                            aTCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCg          >  2:1367666/1‑101 (MQ=255)
                                                                                                gTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGa      >  1:841621/1‑101 (MQ=255)
                                                                                                gTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGa      <  2:2979930/101‑1 (MQ=255)
                                                                                                gTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGa      >  1:1138141/1‑101 (MQ=255)
                                                                                                gTGCTCAGCTTGCCGATCTGCGTCGCACTCAGGCCCGAAGCCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGa      <  2:705908/101‑1 (MQ=255)
                                                                                                gTGCTCAGCGTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGa      <  2:2446229/101‑1 (MQ=255)
                                                                                                 tGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGAc     <  1:3988748/101‑1 (MQ=255)
                                                                                                   cTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAg   >  1:2501290/1‑101 (MQ=255)
                                                                                                   cTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACAGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAg   <  1:1578177/101‑1 (MQ=255)
                                                                                                    tCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAgc  <  1:3842227/101‑1 (MQ=255)
                                                                                                    tCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAgc  >  2:2637866/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
GAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAGC  >  NC_014034/681589‑681789

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG