Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	797,964	G→C	100%	G183G (GGG→GGC)	sucB →	dihydrolipoyllysine‑residue succinyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	797,964	0	G→C	100.0%	221.1	61	G183G (GGG→GGC)	sucB	dihydrolipoyllysine‑residue succinyltransferase
Reads supporting (aligned to +/- strand):  new base (11/49):  ref base (0/1):  total (11/50)

GAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGAA  >  NC_014034/797866‑798059                                                                                                   |                                                                                                gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGGTCCTGGCGGCGGGGTc                                                                                               >  1:1857962/1‑101 (MQ=255) gAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTc                                                                                               <  1:4080030/101‑1 (MQ=255)   ggTTCCGTCCTCGGCGGCGGGCGTCCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTg                                                                                             <  2:1613110/101‑1 (MQ=255)     ttCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGcc                                                                                           <  2:1979289/101‑1 (MQ=255)       ccGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCcgc                                                                                         <  2:607577/101‑1 (MQ=255)       ccGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGGCGTCTGGCGATCCTGGCGGCGGGCTCTGCcgc                                                                                         >  1:999042/1‑101 (MQ=255)          gCCTCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGCCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGc                                                                                      <  2:1000848/101‑1 (MQ=255)          gCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGc                                                                                      <  1:731187/101‑1 (MQ=255)           ccccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCt                                                                                     <  2:3045809/101‑1 (MQ=255)            cccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTc                                                                                    <  2:3283762/101‑1 (MQ=255)             ccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTcc                                                                                   <  1:1726030/101‑1 (MQ=255)             ccGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTcc                                                                                   <  2:154837/101‑1 (MQ=255)              cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCa                                                                                  <  2:3812845/101‑1 (MQ=255)              cggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCa                                                                                  <  1:3314867/101‑1 (MQ=255)               ggcggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAg                                                                                 <  2:3384109/101‑1 (MQ=255)                 cggcggGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGcc                                                                               <  2:4092716/101‑1 (MQ=255)                  ggcgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGccc                                                                              <  1:2796666/101‑1 (MQ=255)                  ggcgggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGGCTGGCGATCCTGGCGGCGGGGTCTGGCGCGGCGCCAGccc                                                                              >  2:3580493/1‑101 (MQ=255)                      ggcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGc                                                                          <  2:1857842/101‑1 (MQ=255)                       gcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGcc                                                                         <  1:404096/101‑1 (MQ=255)                       gcgTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGcc                                                                         <  1:2373448/101‑1 (MQ=255)                       gcgTGCGGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGcc                                                                         <  2:263143/101‑1 (MQ=255)                          tGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCt                                                                      <  1:3442785/101‑1 (MQ=255)                          ggCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCt                                                                      <  1:1241660/100‑1 (MQ=255)                           gCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTg                                                                     <  2:2430534/101‑1 (MQ=255)                             tGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcg                                                                   <  2:2790590/101‑1 (MQ=255)                             tGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcg                                                                   <  2:1056841/101‑1 (MQ=255)                              gAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcgg                                                                  <  2:4018222/101‑1 (MQ=255)                               aGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcggc                                                                 <  2:2210133/101‑1 (MQ=255)                               aGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTgcggc                                                                 <  2:531209/101‑1 (MQ=255)                                 cGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGcggcgg                                                               <  2:1247549/101‑1 (MQ=255)                                  gagaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCggcggc                                                              <  1:1878621/101‑1 (MQ=255)                                  gagaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCCGGcgcggc                                                              >  1:1287973/1‑101 (MQ=255)                                   agaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  1:4112873/101‑1 (MQ=255)                                   agaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  2:2523864/101‑1 (MQ=255)                                   agaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGACCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  1:3580746/101‑1 (MQ=255)                                   acaTCCTCGCCCCCGAGGACCCCTCCGTAACGGCGGGGGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGGGGCTCCAGCCCCGGCCGCTGCGGCGgcc                                                             <  2:657052/99‑1 (MQ=255)                                    gaTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgccg                                                            <  1:914027/101‑1 (MQ=255)                                     aTCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgccgc                                                           <  2:1766855/101‑1 (MQ=255)                                      tCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgccgcc                                                          <  1:1330247/101‑1 (MQ=255)                                      tCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGgccgcc                                                          <  1:2586824/101‑1 (MQ=255)                                       ccTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccc                                                         <  1:937021/101‑1 (MQ=255)                                         tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  2:998951/101‑1 (MQ=255)                                         tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  1:1407148/101‑1 (MQ=255)                                         tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  2:3892880/101‑1 (MQ=255)                                         tCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGccccc                                                       <  1:2425290/101‑1 (MQ=255)                                               ccGAGGGCGCCTCGGTCGCGGCGGGCGGTGGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgccc                                                 <  1:1365925/101‑1 (MQ=255)                                                 gAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGcgcccgc                                               <  2:1611861/101‑1 (MQ=255)                                                   gggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCgcccgccc                                             <  2:2547676/101‑1 (MQ=255)                                                   gggCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCgcccgccc                                             <  2:3964643/101‑1 (MQ=255)                                                     gcgcCTCGGGCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcg                                           <  1:2544736/101‑1 (MQ=255)                                                       gcCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcggc                                         <  2:2923014/101‑1 (MQ=255)                                                       cccTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCGGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCcggc                                         <  1:1933778/100‑1 (MQ=255)                                                              tCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGAGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGccc                                  <  1:605124/101‑1 (MQ=255)                                                                    cgggcggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCCGCGGCGGCCGCCCCCGCGCCCGCCCCGGTGGCGCCCGCGGAg                            >  2:3728701/1‑101 (MQ=255)                                                                        cggTCGTCTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATg                        >  1:2246942/1‑101 (MQ=255)                                                                               cTGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGGCGCGCCCCCGGAGGGTGTCGGACa                 >  1:1457930/1‑101 (MQ=255)                                                                                tGGCGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACAt                <  2:537852/101‑1 (MQ=255)                                                                                 ggCGACCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCGGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATg               <  1:3728955/101‑1 (MQ=255)                                                                                   cGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACCTGcc             >  2:198547/1‑101 (MQ=255)                                                                                   cGATCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGcc             >  2:1073144/1‑101 (MQ=255)                                                                                     aTCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCCCGAAGGATGTCGAACATGcccc           >  2:345274/1‑101 (MQ=255)                                                                                      tCCTGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGccccc          >  1:271278/1‑101 (MQ=255)                                                                                         tGGCGGCGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGGCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCcgcg       >  1:3288695/1‑101 (MQ=255)                                                                                          ggcggcGGGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGGCCCCgcgg      >  2:2904869/1‑101 (MQ=255)                                                                                            cgccagggTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCgcggcg    >  2:2719137/6‑101 (MQ=255)                                                                                             ggcggGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGa   >  2:2443155/1‑101 (MQ=255)                                                                                              gcggGCTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGaa  >  2:1225080/1‑101 (MQ=255)                                                                                                   |                                                                                                GAGGTTCCGGCCCCGGCGGCGGGCGTGCTGAGCGAGATCCTCGCCCCCGAGGGCGCCTCGGTCGCGGCGGGCGGTCGTCTGGCGATCCTGGCGGCGGGGTCTGCCGCGGCTCCAGCCCCGGCCGCTGCGGCGGCCGCCCCCGCGCCCGCCCCGGCGGCGCCCGCGAAGGATGTCGAACATGCCCCCGCGGCGAA  >  NC_014034/797866‑798059 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG