BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,395,397	Δ1 bp	7.8%	intergenic (+209/‑722)	RCAP_rcc01306 → / → RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,395,397	0	T→.	7.8%	53.3 ‑0.2 ‑0.4	257	intergenic (+209/‑722)	RCAP_rcc01306/RCAP_rcc01307	Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand): new base (12/8): ref base (129/107): total (142/115)
Fisher's exact test strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.88e-01

Only 100 of 280 total aligned reads displayed.


CTTGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC  >  NC_014034/1395297‑1395497


cTTGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                       <  1:1048533/101‑1 (MQ=255)


cTTGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                                                       <  1:1385315/101‑1 (MQ=255)


  tGCGACAAGCGCCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                     <  2:3449542/101‑1 (MQ=255)


  tGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                     >  2:4168302/1‑101 (MQ=255)


  tGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                     >  2:4334021/1‑101 (MQ=255)


  tGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                     >  1:4180735/1‑101 (MQ=255)


  tGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGc                                                                                                     >  1:1991616/1‑101 (MQ=255)


   gCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCTCGGGGGCGTGGATCGAAACTGCa                                                                                                    >  2:1467914/1‑101 (MQ=255)


   gCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCTGGGGCGTGGATCGAAACTGCa                                                                                                    >  1:1144922/1‑101 (MQ=255)


    cGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAc                                                                                                   <  1:4330052/101‑1 (MQ=255)


     gACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAcc                                                                                                  <  1:3897445/101‑1 (MQ=255)


     gACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAcc                                                                                                  >  1:1450286/1‑101 (MQ=255)


      aCAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCAccc                                                                                                 <  2:3880623/101‑1 (MQ=255)


         aGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCt                                                                                              <  1:2480479/101‑1 (MQ=255)


          gCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTg                                                                                             >  1:2413803/1‑101 (MQ=255)


              cTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGccc                                                                                         >  2:3507999/1‑101 (MQ=255)


               ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                        >  1:2979026/1‑101 (MQ=255)


               ttCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCt                                                                                        <  2:2413992/101‑1 (MQ=255)


                tCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGATACGAAACGGCAGTCGCTCCCCCCTCGGGGGCGTGGATCGGAAATGCACCCGCCAGCCCTg                                                                                       >  2:3978216/1‑101 (MQ=255)


                 cGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCAGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGc                                                                                      >  2:1828004/1‑101 (MQ=255)


                  gCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGcc                                                                                     >  2:3750790/1‑101 (MQ=255)


                   caccacCGGAGCAATTTACACCGTCTGGTCAGCTGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                    <  1:3999730/101‑1 (MQ=255)


                   caccacCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcg                                                                                    >  2:2342682/1‑101 (MQ=255)


                        ccGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgat                                                                               <  2:3918977/101‑1 (MQ=255)


                         cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatc                                                                              >  2:1874739/1‑101 (MQ=255)


                         cGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatc                                                                              <  1:1524043/101‑1 (MQ=255)


                          ggAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcg                                                                             >  2:2061357/1‑101 (MQ=255)


                          ggAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcg                                                                             >  2:2110342/1‑101 (MQ=255)


                          ggAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcg                                                                             >  2:661631/1‑101 (MQ=255)


                           gAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgg                                                                            >  1:2350769/1‑101 (MQ=255)


                           gAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgg                                                                            <  2:396676/101‑1 (MQ=255)


                            aGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggt                                                                           >  1:2986407/1‑101 (MQ=255)


                              cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcgggg.a                                                                        >  2:1389407/1‑101 (MQ=255)


                              cAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggcgatcggtg.a                                                                        >  1:3469367/1‑101 (MQ=255)


                               aaTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCcggtgatcggtg.at                                                                       <  2:2024427/101‑1 (MQ=255)


                                aTTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCggtgatcggtg.atc                                                                      >  1:3878072/1‑101 (MQ=255)


                                 tttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGGGATCGGTG.ATCa                                                                     >  2:4031935/1‑101 (MQ=255)


                                  ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGGCCTGCCGGTGATCGGTG.ATCAg                                                                    >  1:4574207/1‑101 (MQ=255)


                                  ttACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAg                                                                    >  1:2888223/1‑101 (MQ=255)


                                    acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                  >  2:1755697/1‑101 (MQ=255)


                                    acacCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCg                                                                  >  2:1791397/1‑101 (MQ=255)


                                      acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                >  1:394741/1‑101 (MQ=255)


                                      acCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGt                                                                >  2:3220691/1‑101 (MQ=255)


                                        cGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCg                                                              <  1:823746/101‑1 (MQ=255)


                                         gTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGc                                                             <  1:1874865/101‑1 (MQ=255)


                                          tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                            >  1:182349/1‑101 (MQ=255)


                                          tCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCt                                                            >  2:4224089/1‑101 (MQ=255)


                                           cTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTc                                                           <  1:3664559/101‑1 (MQ=255)


                                               tCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTccccc                                                       >  1:3200532/1‑101 (MQ=255)


                                                 aGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcg                                                     <  1:3292896/101‑1 (MQ=255)


                                                  gCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCcgc                                                    <  1:3417436/101‑1 (MQ=255)


                                                    gggTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgg                                                  <  1:1260656/101‑1 (MQ=255)


                                                      gTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCgggg                                                <  1:2338731/101‑1 (MQ=255)


                                                       ttACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCggggg                                               <  2:2978797/101‑1 (MQ=255)


                                                        tgcGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                              <  2:1707739/99‑1 (MQ=255)


                                                        tACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGc                                              <  2:1193667/101‑1 (MQ=255)


                                                          cGAAACGGCAGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGGCCTGCCGGCGCTCGGTG.ATCAGCGGTCTCTCTTCCCGCGGGGGCGt                                            >  2:3080792/1‑101 (MQ=37)


                                                           gAAACGGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTg                                           <  2:3244209/101‑1 (MQ=255)


                                                            tcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGAt                                        >  1:4524058/9‑101 (MQ=2)


                                                            aaaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.CTCAGCGGGCGCTCCCCCCCCGGGGGCGTgg                                          >  2:4519730/1‑101 (MQ=255)


                                                             caacactgTCGCACCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                       >  1:2528256/8‑101 (MQ=2)


                                                             aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                         >  1:4494640/1‑101 (MQ=255)


                                                             aaCGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGa                                         <  1:1814703/101‑1 (MQ=255)


                                                               cGGCAGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATc                                       <  2:3281574/101‑1 (MQ=255)


                                                                cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    >  2:1610708/5‑101 (MQ=2)


                                                                cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC.GCTGGCGCGCGCCGCGCCG..GATCTTTGTCTGCGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    >  1:4390574/5‑101 (MQ=2)


                                                                 gCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGa                                     <  1:749198/101‑1 (MQ=255)


                                                                  cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    <  2:3004297/101‑1 (MQ=255)


                                                                  cAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    <  2:4471129/101‑1 (MQ=255)


                                                                  cAGACGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGATGGACCTGCCGGTGATCGGTG.ATCAGCGGTCGCACCCCCCGCGGGGGCGTGGATCGaa                                    >  2:438207/1‑101 (MQ=38)


                                                                   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                   >  1:939820/1‑101 (MQ=255)


                                                                     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                 <  1:1755816/101‑1 (MQ=255)


                                                                      cGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACAt                                >  1:592815/1‑101 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                               <  1:1173799/101‑1 (MQ=255)


                                                                       gCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                               >  2:2564729/1‑101 (MQ=255)


                                                                        cTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcg                              >  1:3751117/1‑101 (MQ=255)


                                                                         tCCCCCCGCGGGGGCGTGGATCGAACCTGCACCCTCTTGCCCTGCCAGCGATCGGTG.CCCAGCCGCCGCCCCCCCCGCCGGTGCCGGGATCGGCACATcgc                             >  2:1098335/1‑101 (MQ=16)


                                                                          ccccccGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATcgcc                            <  1:1019681/101‑1 (MQ=255)


                                                                                 cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                     >  2:291490/1‑101 (MQ=255)


                                                                                 cgGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTgg                     >  2:2237069/1‑101 (MQ=255)


                                                                                   ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                   >  2:3152187/1‑101 (MQ=255)


                                                                                   ggggCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCg                   >  1:2100205/1‑101 (MQ=255)


                                                                                      gCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTc                <  1:3508257/101‑1 (MQ=255)


                                                                                       cGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCg               >  2:1371769/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              >  1:2759213/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              >  1:41322/1‑101 (MQ=255)


                                                                                        gTGGATCGAAACCGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGa              <  2:2742043/101‑1 (MQ=255)


                                                                                        gTGGAGCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGTTCTGTCCCGCCGCGGGGGCGTGGATCGACATATCGCCGCTTTGGCGGTCGa              >  2:806072/1‑101 (MQ=255)


                                                                                         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGGGTGGATCGAAACATCGCCGCTTTGGCGGTAGAc             >  1:1643886/1‑101 (MQ=255)


                                                                                         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc             >  2:2985953/1‑101 (MQ=255)


                                                                                         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGTAATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGAc             <  1:3981298/101‑1 (MQ=255)


                                                                                         tGGATCGAAACTGCACCCGCTGGCCCTGCCGGCGATCGGTG.ATCAGCGGTCCCTCCCCCCGCGGGGGGGTGGATCGAAACATCGCCGCTTTGGCGGGCGAc             >  1:623051/1‑101 (MQ=255)


                                                                                           gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg           <  1:4319545/101‑1 (MQ=255)


                                                                                           gATCGAAACTGCCCCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg           <  1:3081036/101‑1 (MQ=255)


                                                                                           gATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAg           >  2:2815872/1‑101 (MQ=255)


                                                                                                 aaCTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGt     <  2:4587914/101‑1 (MQ=255)


                                                                                                    tGCCCCCGCTGGCCCTGGGGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  <  1:4646387/101‑1 (MQ=255)


                                                                                                    tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  <  1:4260757/101‑1 (MQ=255)


                                                                                                    tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  <  1:4413331/101‑1 (MQ=255)


                                                                                                    tGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAc  >  2:3542194/1‑101 (MQ=255)


CTTGCGACAAGCACCTTCGCACCACCGGAGCAATTTACACCGTCTGGTCAGCGGGTTACGAAACGGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCACCCGCTGGCCCTGCCGGTGATCGGTG.ATCAGCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCGCCGCTTTGGCGGTCGACAGGCTGGTAAC  >  NC_014034/1395297‑1395497

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG