Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,698 Δ1 bp 2.9% intergenic (+2/+1896) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6980A→. 2.9% 9.4 0.0 ‑0.0205intergenic (+2/+1896)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (107/92):  total (110/95)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.93e-01

Only 100 of 220 total aligned reads displayed.

CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798
                                                                                                      |                                                                                                    
cATCCGCGGATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACa                                                                                                      <  1:2334174/101‑1 (MQ=255)
 ttcgccgGGTCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCAAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAg                                                                                                     <  2:3296416/96‑1 (MQ=255)
 aTCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAg                                                                                                     >  1:1783384/1‑101 (MQ=255)
   ccGGGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGt                                                                                                   <  1:1538015/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTc                                                                                                  >  1:416746/1‑101 (MQ=255)
     gcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCg                                                                                                 >  2:2744050/1‑101 (MQ=255)
      cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGc                                                                                                >  1:1356085/1‑101 (MQ=255)
       ggATCGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGcc                                                                                               <  2:2421379/101‑2 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                                               <  1:4614937/101‑1 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                                               >  2:2076656/1‑101 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCt                                                                                               >  2:938375/1‑101 (MQ=255)
          tCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccc                                                                                            >  1:1632769/1‑101 (MQ=255)
          tCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccc                                                                                            <  1:524712/101‑1 (MQ=255)
           cGGCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTTTGTTC.CGGTAACAGGTCGCTcccc                                                                                           <  2:352170/101‑1 (MQ=255)
           cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccc                                                                                           <  1:4543174/101‑1 (MQ=255)
           cGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccc                                                                                           >  2:1384901/1‑101 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTccccc                                                                                          >  2:3443227/1‑101 (MQ=255)
             gCGTGACCCGCCCCGCCCATCGGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTcccccc                                                                                         <  1:2809362/101‑1 (MQ=255)
               gTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgc                                                                                       >  2:3924029/1‑101 (MQ=255)
                ggtcccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCcgcg                                                                                      <  1:4481513/98‑1 (MQ=255)
                  gcccgccccgcGCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAAAATTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCggg                                                                                    <  1:3135354/100‑1 (MQ=255)
                   cccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCgggg                                                                                   <  2:1045227/101‑2 (MQ=255)
                     cgccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGgggc                                                                                 <  1:3441129/101‑1 (MQ=255)
                      gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                >  1:1982364/1‑101 (MQ=255)
                        cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                                              <  1:714580/101‑1 (MQ=255)
                        cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                                              >  2:3016964/1‑101 (MQ=255)
                          cgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            >  2:2090134/1‑101 (MQ=255)
                           gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           >  1:853459/1‑101 (MQ=255)
                           gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  2:616186/101‑1 (MQ=255)
                            cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          >  1:4324836/1‑101 (MQ=255)
                             ccATCGGCCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  1:174354/101‑1 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:4129834/101‑1 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       >  2:2247622/1‑101 (MQ=255)
                               aTCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                       <  2:3171759/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  2:1535387/1‑101 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  2:528072/1‑101 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                                                   <  1:2228017/101‑1 (MQ=255)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTc                                                                <  1:1906829/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                                               <  1:3868355/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                                               <  2:2361011/101‑1 (MQ=255)
                                        tcgcgccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                              <  1:297372/96‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTGGTTCTGTTC.CGGTAACAGGGCGCACCCCCCGCGGGGGCGTGGATCGAAACTTGTCTg                                                              >  1:3990477/1‑101 (MQ=38)
                                          cccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAc                                                            <  1:106312/101‑1 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcg                                                           <  1:157802/101‑1 (MQ=255)
                                            cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggg                                                          >  1:3934782/1‑96 (MQ=0)
                                            cccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggc                                                          <  1:3366579/101‑6 (MQ=0)
                                             ccGCGGGGGCGTGGATCGAAACGGCGTAGATCTCGGTCTGGGTGCGCTCGCGGTTGC..GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCtatggcg                                                         >  1:1082998/1‑95 (MQ=0)
                                                cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                      >  2:2650603/1‑101 (MQ=255)
                                                cgGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtc                                                      >  2:2859784/1‑101 (MQ=255)
                                                 ggtggCCTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     <  1:1450169/98‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     <  1:591543/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   >  1:3286185/1‑101 (MQ=255)
                                                   gggCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   >  1:2445660/1‑101 (MQ=255)
                                                     gCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCGCATCAg                                                 >  2:1451283/1‑101 (MQ=255)
                                                      cGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGc                                                >  2:3864592/1‑101 (MQ=255)
                                                       gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                               >  2:2166038/1‑101 (MQ=255)
                                                       gTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                               >  1:4476707/1‑101 (MQ=255)
                                                        tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              >  2:1776332/1‑101 (MQ=255)
                                                        tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              >  2:800143/1‑101 (MQ=255)
                                                        tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              <  2:752771/101‑1 (MQ=255)
                                                        tGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              >  1:948296/1‑101 (MQ=255)
                                                        gggATCGCAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              <  2:4556224/100‑1 (MQ=255)
                                                         ggATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             <  2:853383/101‑1 (MQ=255)
                                                              gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  1:938455/101‑1 (MQ=255)
                                                              gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        >  2:4326331/1‑101 (MQ=255)
                                                              gAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  1:356775/101‑1 (MQ=255)
                                                                aaCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                                      <  1:3666844/101‑1 (MQ=255)
                                                                 aCTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                     >  2:3112680/1‑101 (MQ=255)
                                                                    aCAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAg                                  >  1:2795168/1‑101 (MQ=255)
                                                                     cAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGt                                 >  2:1826848/1‑101 (MQ=255)
                                                                      agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                >  2:2553752/1‑101 (MQ=255)
                                                                      agagTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGGCAAGATTCAGTc                                >  1:1045386/1‑101 (MQ=255)
                                                                      agagTGC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                <  2:722772/101‑1 (MQ=255)
                                                                        agTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              >  2:1029787/1‑101 (MQ=255)
                                                                         gTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCt                             <  2:3769789/101‑1 (MQ=255)
                                                                          ttC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGGGGATCGACACTTGTCTGACGCGCTCATCAGAAGGTCAAGATTCAGTCGCTc                            >  1:4416581/1‑101 (MQ=38)
                                                                           tC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc                           <  1:2631034/101‑1 (MQ=255)
                                                                               ggTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                        >  2:1211778/1‑101 (MQ=255)
                                                                                gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       <  2:1632664/101‑1 (MQ=255)
                                                                                gTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       <  1:2559895/101‑1 (MQ=255)
                                                                                     gttctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                  >  1:2760447/1‑101 (MQ=255)
                                                                                       tctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                >  1:395013/1‑101 (MQ=255)
                                                                                       tctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                >  1:3715180/1‑101 (MQ=255)
                                                                                       tctcttc.CGGTAACAGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                <  1:1939773/101‑1 (MQ=255)
                                                                                        ctgttc.CGGTAACGGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:666927/101‑1 (MQ=255)
                                                                                        ctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:132736/101‑1 (MQ=255)
                                                                                        ctgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:1805646/101‑1 (MQ=255)
                                                                                         tgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg              >  2:3368287/1‑101 (MQ=255)
                                                                                         tgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg              >  2:1497981/1‑101 (MQ=255)
                                                                                         tgttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCg              >  2:2085869/1‑101 (MQ=255)
                                                                                          gttc.CGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCCCCGGGGCgg             >  1:3178162/1‑100 (MQ=255)
                                                                                               cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATGGAAACTTGTCTGACGCGCTCATCAGCAAGTCAAGATTAAGTCGCTCCCCCCCCGGGGGCGTGGAt         >  1:3831785/1‑101 (MQ=38)
                                                                                               cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAt         <  2:3149095/101‑1 (MQ=255)
                                                                                               cGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGAt         <  1:2076796/101‑1 (MQ=255)
                                                                                                ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc        <  1:3915071/101‑1 (MQ=255)
                                                                                                 gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       <  1:2074519/101‑1 (MQ=255)
                                                                                                  tAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGa      >  1:667181/1‑101 (MQ=255)
                                                                                                      aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  2:3990610/1‑100 (MQ=255)
                                                                                                      aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  2:3614685/1‑100 (MQ=255)
                                                                                                      aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  1:3405207/1‑100 (MQ=255)
                                                                                                      |                                                                                                    
CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTC.CGGTCTTGTTCTGTTC.CGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG