Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,960,668 Δ1 bp 96.0% coding (1111/1539 nt) RCAP_rcc01813 ← AMP‑dependent synthetase and ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,960,6680G→.96.0% 20.8 ‑0.7 ‑1.4225coding (1111/1539 nt)RCAP_rcc01813AMP‑dependent synthetase and ligase
Reads supporting (aligned to +/- strand):  new base (93/123):  ref base (5/4):  total (98/127)
Fisher's exact test strand distribution p-value = 5.08e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.35e-02

Only 100 of 235 total aligned reads displayed.

CCTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGAC  >  NC_014034/1960569‑1960764
                                                                                                    |                                                                                                
ccTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTcggcg                                                                                                  <  2:1412068/101‑1 (MQ=255)
  tGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTcggcgcc                                                                                                >  1:4193043/1‑99 (MQ=255)
  tGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTcggcgcc                                                                                                >  2:4215326/1‑99 (MQ=255)
     tAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGCGCccca                                                                                             <  2:2826315/101‑2 (MQ=255)
     tAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGCGCccca                                                                                             <  2:3521079/101‑2 (MQ=255)
       ggCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAAt                                                                                          <  2:2880450/101‑1 (MQ=255)
        gCGGTGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATa                                                                                         <  1:4409679/101‑1 (MQ=255)
         cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAg                                                                                        <  1:3378065/101‑1 (MQ=255)
         cGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAg                                                                                        <  1:2160018/101‑1 (MQ=255)
           aTGGCCCCGGCCTCGGTCATCCGCACGGTGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGcc                                                                                      <  2:3728411/101‑1 (MQ=255)
            tGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCg                                                                                     <  2:4605262/101‑1 (MQ=255)
              gCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAg                                                                                   <  1:3184015/101‑1 (MQ=255)
               ccccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTTAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAgg                                                                                  <  1:3053249/101‑1 (MQ=255)
                cccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGa                                                                                 <  2:4243165/101‑1 (MQ=255)
                cccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGa                                                                                 <  2:571089/101‑1 (MQ=255)
                 ccGGCCTCGGTCATCCGCTCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaa                                                                                <  1:934985/101‑1 (MQ=255)
                 ccGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGaa                                                                                <  1:3116957/101‑1 (MQ=255)
                    gCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAg                                                                             >  2:2778892/1‑101 (MQ=255)
                    gCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCACGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAg                                                                             <  2:4459577/101‑1 (MQ=255)
                      cTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCGGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAgac                                                                           >  1:2272259/1‑99 (MQ=255)
                       tCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGccc                                                                          >  2:2133333/1‑101 (MQ=255)
                        cGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGcccc                                                                         <  2:1227162/101‑1 (MQ=255)
                        cGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGcccc                                                                         <  1:1778291/101‑1 (MQ=255)
                         ggTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCg                                                                        <  1:1381999/101‑1 (MQ=255)
                          gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                       <  1:2424104/101‑1 (MQ=255)
                          gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCgg                                                                       <  1:1522271/101‑1 (MQ=255)
                          gTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGAGGTGAACTTTGCCCGCGCCGCGGACTCGGC.GCCCCAATAGCCGAGGAAAAACCCCgg                                                                       >  1:3985922/1‑101 (MQ=255)
                            cATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGAt                                                                     <  2:3763941/101‑1 (MQ=255)
                             aTCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATc                                                                    <  2:4398040/101‑1 (MQ=255)
                              tCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCg                                                                   >  1:422438/1‑101 (MQ=255)
                                cGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCt                                                                 <  1:247622/101‑1 (MQ=255)
                                 gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                <  2:2757095/101‑1 (MQ=255)
                                 gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                <  1:4255260/101‑1 (MQ=255)
                                 gCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTg                                                                >  2:4065115/1‑101 (MQ=255)
                                  cACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGc                                                               <  2:1870358/101‑1 (MQ=255)
                                  cACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGc                                                               <  2:472008/101‑1 (MQ=255)
                                   aCGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCa                                                              >  2:2636177/1‑101 (MQ=255)
                                       tGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGc                                                          <  1:2307243/101‑1 (MQ=255)
                                         tCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCAc                                                        <  1:36356/101‑1 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                      >  1:1272599/1‑101 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                      <  1:2400500/101‑1 (MQ=255)
                                           gCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCg                                                      <  2:1177823/101‑1 (MQ=255)
                                            ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGa                                                     <  2:1494881/101‑1 (MQ=255)
                                            ccGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGa                                                     <  1:2323195/101‑1 (MQ=255)
                                             gggTGCGGAGCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                    <  2:4322866/100‑1 (MQ=255)
                                             cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                    <  1:2187849/101‑1 (MQ=255)
                                             cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                    <  1:2567408/101‑1 (MQ=255)
                                             cGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGAc                                                    <  1:846014/101‑1 (MQ=255)
                                              ggTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACa                                                   >  2:2182097/1‑101 (MQ=255)
                                                tGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGc                                                 <  1:754944/101‑1 (MQ=255)
                                                tGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGc                                                 <  1:2747687/101‑1 (MQ=255)
                                                  cGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTc                                               >  2:76661/1‑101 (MQ=255)
                                                   ggAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCg                                              <  2:2505989/101‑1 (MQ=255)
                                                    gAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGc                                             <  1:4000519/101‑1 (MQ=255)
                                                     aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                            >  2:1095858/1‑101 (MQ=255)
                                                     aaCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGcc                                            <  2:681633/101‑1 (MQ=255)
                                                      aCCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGccc                                           <  2:3963225/101‑1 (MQ=255)
                                                       ccAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGTAATGCACCGACAGCTCGCCCg                                          <  2:2602959/101‑1 (MQ=255)
                                                       ccAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCg                                          >  2:2173075/1‑101 (MQ=255)
                                                         aGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCTGGt                                        <  2:3153492/101‑1 (MQ=255)
                                                          gTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGtt                                       <  2:3844161/101‑1 (MQ=255)
                                                          gTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGtt                                       <  1:4585923/101‑1 (MQ=255)
                                                           tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  2:1141835/101‑1 (MQ=255)
                                                           tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  1:2799124/101‑1 (MQ=255)
                                                           tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  1:4638992/101‑1 (MQ=255)
                                                           tCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGGACTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTc                                      <  1:474472/101‑1 (MQ=255)
                                                            ccccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCNACAGCTCGCCCGGntgg                                     >  2:2061165/1‑99 (MQ=255)
                                                             cccGTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCCGCAATGCACCGACAGCTCGCCCGGTTCGc                                    <  2:4351678/101‑1 (MQ=255)
                                                                gTCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGcccc                                 >  1:3737821/1‑101 (MQ=255)
                                                                 tCCGCGGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCg                                <  1:2948655/101‑1 (MQ=255)
                                                                   cgcgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCggg                              >  1:1198716/1‑101 (MQ=255)
                                                                    gcgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgggg                             <  1:1164237/101‑1 (MQ=255)
                                                                    gcgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCgggg                             >  1:4319994/1‑101 (MQ=255)
                                                                     cgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGCCAGCTCGCCCGCTTCGCCCCGGGGc                            >  2:4100764/1‑101 (MQ=255)
                                                                     cgGTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGc                            >  1:2727185/1‑101 (MQ=255)
                                                                      ggggaccTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                           <  2:602894/95‑1 (MQ=255)
                                                                      ggTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                           >  1:4535725/1‑101 (MQ=255)
                                                                      ggTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCa                           <  2:273894/101‑1 (MQ=255)
                                                                       gTGAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCAc                          <  1:3501657/101‑1 (MQ=255)
                                                                         gAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCg                        <  1:3401740/101‑1 (MQ=255)
                                                                         gAACTTTGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCg                        >  1:3836351/1‑101 (MQ=255)
                                                                           aCTTTGCCCGCGCCTCGGCCTCAGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGa                      <  1:2173225/101‑1 (MQ=255)
                                                                             tttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                    >  1:1072065/1‑101 (MQ=255)
                                                                             tttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATg                    <  2:3330401/101‑1 (MQ=255)
                                                                             tttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGATCGCCCGGTTCGCCCCGGGGCACCGGATg                    >  2:1770716/1‑101 (MQ=255)
                                                                              ttGCCCGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATgg                   <  2:1190531/101‑1 (MQ=255)
                                                                                  ccGCGCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccct               <  1:115092/101‑3 (MQ=255)
                                                                                   cgcgCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCATCGGATGGccctc              <  1:2534895/101‑4 (MQ=255)
                                                                                   cgcgCCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctc              <  2:2314854/101‑4 (MQ=255)
                                                                                    gcgcCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctcg             <  1:1885772/101‑5 (MQ=255)
                                                                                     cgcCTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGccctcgg            <  1:2665680/101‑6 (MQ=255)
                                                                                       ccTCGGCCTCGGC.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCgggg          >  1:3313575/1‑101 (MQ=255)
                                                                                         tcggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa        <  2:915794/96‑1 (MQ=255)
                                                                                         tcggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGa        <  1:768632/96‑1 (MQ=255)
                                                                                           ggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa      >  1:4440542/4‑101 (MQ=255)
                                                                                           ggcctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACa      >  1:2868902/4‑101 (MQ=255)
                                                                                              ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGa   <  1:436195/101‑1 (MQ=255)
                                                                                              ctcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGa   >  2:829422/1‑101 (MQ=255)
                                                                                               tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGCCAGGAc  >  2:3422259/1‑101 (MQ=255)
                                                                                               tcggc.GCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCCTCGGGGGACAGGAc  <  2:2977905/101‑1 (MQ=255)
                                                                                                    |                                                                                                
CCTGATAGGCGATGGCCCCGGCCTCGGTCATCCGCACGATGTCGCCGGTGCGGAACCAGTCCCCGTCCGCGGTGAACTTTGCCCGCGCCTCGG.CTCGGCGGCCCCAATAGCCGAGGAAAAGCCCCGGATCGCTGCAATGCACCGACAGCTCGCCCGGTTCGCCCCGGGGCACCGGATGGCCTCCGGGGGACAGGAC  >  NC_014034/1960569‑1960764

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG