Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 290,303 Δ1 bp 3.1% intergenic (‑36/+14) RCAP_rcc00253 ← / ← RCAP_rcc00254 major facilitator superfamily protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034290,3030G→. 3.1% 10.9 0.0 ‑0.0196intergenic (‑36/+14)RCAP_rcc00253/RCAP_rcc00254major facilitator superfamily protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/3):  ref base (95/95):  total (98/98)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.11e-01

Only 100 of 234 total aligned reads displayed.

AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403
                                                                                                    |                                                                                                    
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     >  2:3776096/1‑101 (MQ=255)
 aGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgg                                                                                                     <  1:3619188/101‑1 (MQ=255)
       gCAGCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCgagcggat                                                                                               <  2:1007798/101‑8 (MQ=255)
       acATCAAGATTGTGGCCCCCCCCAAAAGGGCGGGGGTGATCAGGCGGGTTTGGGTCATTTGGTCCCCCCCCCAAGGCGGCCAGTTCCCCATGGgggggggg                                                                                               <  2:3513002/100‑1 (MQ=255)
        cATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCggggggggg                                                                                              <  1:3108801/101‑1 (MQ=255)
            aaGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGc                                                                                          >  1:1493249/1‑101 (MQ=255)
             aGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGACTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCg                                                                                         >  2:2381860/1‑101 (MQ=255)
                tgatgGCGCCGCCCACAAGGACGGGGNTGANCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCa                                                                                      <  1:3757971/101‑1 (MQ=255)
                 gatgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCaa                                                                                     >  2:1690888/1‑101 (MQ=255)
                   tgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATc                                                                                   >  1:2050525/1‑101 (MQ=255)
                   tgGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATc                                                                                   >  1:2257845/1‑101 (MQ=255)
                    ggCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCt                                                                                  >  2:3608270/1‑101 (MQ=255)
                      cgccgcCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGt                                                                                >  2:3298306/1‑101 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  2:891132/101‑1 (MQ=255)
                       gccgccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTc                                                                               <  2:488132/101‑1 (MQ=255)
                          gccCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGAt                                                                            >  2:1529141/1‑101 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          >  2:2619580/1‑101 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          >  2:2168231/1‑101 (MQ=255)
                            ccACAAGGACGGGGGTGATCAGGCGGGTCTGGGGCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATtg                                                                          >  2:1196662/1‑101 (MQ=255)
                               caAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGc                                                                       <  1:1555574/101‑1 (MQ=255)
                               caAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGcc                                                                      >  1:3388335/1‑101 (MQ=255)
                                aaGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcc                                                                      >  1:1856943/1‑101 (MQ=255)
                                 aGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGccc                                                                     >  1:4489672/1‑101 (MQ=255)
                                  ggACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGcccc                                                                    >  2:2767776/1‑101 (MQ=255)
                                   gACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   >  2:2344355/1‑101 (MQ=255)
                                   gACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCg                                                                   >  2:3864761/1‑101 (MQ=255)
                                    aCGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGa                                                                  >  1:403179/1‑101 (MQ=255)
                                     cGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGaa                                                                 >  2:4122987/1‑101 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:440790/101‑1 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                >  2:2835460/1‑101 (MQ=255)
                                      gggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAAc                                                                <  1:2756830/101‑1 (MQ=255)
                                       ggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACaa                                                              >  2:1563966/1‑101 (MQ=255)
                                       ggggTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACaa                                                              <  2:4334237/101‑1 (MQ=255)
                                          gggATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATc                                                            <  1:3021618/99‑1 (MQ=255)
                                          gTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATc                                                            <  1:284803/101‑1 (MQ=255)
                                           tGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcg                                                           <  1:355824/101‑1 (MQ=255)
                                            gATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTTTGCCCCCAACAATcgc                                                          >  1:1585487/1‑101 (MQ=255)
                                             aTCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATcgcg                                                         <  1:366085/101‑1 (MQ=255)
                                             aTCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGC.GGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCgcgc                                                        <  2:367687/101‑1 (MQ=255)
                                              tCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCgcgc                                                        >  1:1017681/1‑101 (MQ=255)
                                               cAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgcc                                                       >  1:3915781/1‑101 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGGCCCGAACAATCGcgccg                                                      >  2:2108727/1‑101 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccg                                                      >  1:535736/1‑101 (MQ=255)
                                                aGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccg                                                      >  1:2379954/1‑101 (MQ=255)
                                                 ggCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgc                                                     >  1:2273086/1‑101 (MQ=255)
                                                   cGGGTCGGGGTCATTTGGTCTCCCTCGAAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccg                                                   <  2:4474781/101‑1 (MQ=255)
                                                    gggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGcgccgccgc                                                  >  2:1799254/1‑101 (MQ=255)
                                                     ggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgcc                                                 <  1:4647151/101‑1 (MQ=255)
                                                     ggTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCgccgccgcc                                                 >  1:3800525/1‑101 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  2:955332/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  1:3460414/101‑1 (MQ=255)
                                                      gTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCt                                                <  1:1593373/101‑1 (MQ=255)
                                                       tCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTg                                               >  1:2694234/1‑101 (MQ=255)
                                                        cTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCTGCCTGc                                              >  1:754696/1‑101 (MQ=255)
                                                        cTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGc                                              <  1:1418211/101‑1 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  1:4059454/1‑101 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             >  1:3359383/1‑101 (MQ=255)
                                                         tGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGcc                                             <  2:3017623/101‑1 (MQ=255)
                                                           ggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCg                                           >  2:319086/1‑101 (MQ=255)
                                                           ggTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCCCCGCCGCCTGCCCg                                           >  2:2468956/1‑101 (MQ=255)
                                                            gTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGACATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCCCCTGccccg                                          >  2:3081481/1‑99 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        >  2:3127285/1‑101 (MQ=255)
                                                              cATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCg                                        <  1:3053996/101‑1 (MQ=255)
                                                               aTTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCTGCGa                                       >  1:714590/1‑101 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     <  1:979274/101‑1 (MQ=255)
                                                                 ttGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGAcg                                     >  2:3407856/1‑101 (MQ=255)
                                                                   ggTCTCCCTCGCACGGCGGCCAGTGCGCCATCCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAGCAATCGCGCCGCCGCCTGCCCGGCGAcgcg                                   <  2:761213/101‑1 (MQ=255)
                                                                   ggTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCCCCTGCCCGGCGAcgcg                                   >  2:56330/1‑101 (MQ=255)
                                                                    gTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCCCCTGCCCCGCGACGCgg                                  >  2:4280256/1‑101 (MQ=255)
                                                                     tctcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGc                                 >  1:265103/1‑101 (MQ=255)
                                                                       tcCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               <  1:2317238/101‑1 (MQ=255)
                                                                       ccccGCGCAGGGCGGCCAGTTCCCCAGGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGccc                               <  1:973781/100‑1 (MQ=255)
                                                                        cccTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCg                              >  1:3700597/1‑101 (MQ=255)
                                                                          cTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTc                            <  1:4123273/101‑1 (MQ=255)
                                                                           tCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCg                           <  1:4337243/101‑1 (MQ=255)
                                                                            cGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGCCGc                          >  2:1955618/1‑101 (MQ=255)
                                                                              cAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCa                        >  1:4311905/1‑101 (MQ=255)
                                                                                aGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTTTGCCCCGAAAAATCGCGCCGCCCCCTGCCCGGCGACGCGGCCCGCCGcccgc                      >  1:3488442/1‑98 (MQ=255)
                                                                                  gggcccgtttCCCCAGGGGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACa                    <  2:1892598/93‑1 (MQ=255)
                                                                                  gcggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACa                    >  2:1752928/1‑101 (MQ=255)
                                                                                   cggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAg                   >  2:2955814/1‑101 (MQ=255)
                                                                                    ggcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGGCGCCAGACAGc                  >  2:3903325/1‑101 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGcc                 <  2:2854485/101‑1 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGcc                 <  2:2020931/101‑1 (MQ=255)
                                                                                     gcCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCACGGCGACGCGGCCCGTCGCCAGACAGcc                 <  1:3180717/101‑1 (MQ=255)
                                                                                        aGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGt              >  2:1247411/1‑101 (MQ=255)
                                                                                         gTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTc             <  1:1196754/101‑1 (MQ=255)
                                                                                          ttCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTca            <  2:2719599/101‑1 (MQ=255)
                                                                                             gCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagca         >  2:1069026/1‑101 (MQ=255)
                                                                                             gCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagca         >  2:4244181/1‑101 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        >  2:4015754/1‑101 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        >  1:634975/1‑101 (MQ=255)
                                                                                              ccATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTcagcag        <  1:2554714/101‑1 (MQ=255)
                                                                                               cATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCACACAGCCGGTCAGCAGa       >  2:1724406/1‑101 (MQ=255)
                                                                                                aTGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGAt      <  1:1247504/101‑1 (MQ=255)
                                                                                                 ggggggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATa     <  1:1189795/98‑1 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgc   >  1:2018122/1‑101 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgc   >  2:2542893/1‑101 (MQ=255)
                                                                                                   cGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGGCAGCAGATAgc   >  1:852591/1‑101 (MQ=255)
                                                                                                    gggggggggCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAgcc  <  1:917997/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
AAGCCAAGCATCAAGATGATGGCGCCGCCCACAAGGACGGGGGTGATCAGGCGGGTCTGGGTCATTTGGTCTCCCTCGCAAGGCGGCCAGTTCGCCATGCGGGGGGGGGCTGCGTCAATCTGTCGATTGTGCCCCGAACAATCGCGCCGCCGCCTGCCCGGCGACGCGGCCCGTCGCCAGACAGCCGGTCAGCAGATAGCC  >  NC_014034/290203‑290403

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG