Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 568,802 Δ1 bp 4.8% intergenic (‑98/+68) RCAP_rcc00530 ← / ← carB hypothetical protein/carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034568,8020C→. 4.8% 17.7 ‑0.7 ‑0.5168intergenic (‑98/+68)RCAP_rcc00530/carBhypothetical protein/carbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (6/2):  ref base (95/64):  total (101/67)
Fisher's exact test strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.93e-01

Only 100 of 230 total aligned reads displayed.

CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568702‑568901
                                                                                                     |                                                                                                   
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                     >  1:388320/1‑101 (MQ=255)
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                     <  1:3055321/101‑1 (MQ=255)
cATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.c                                                                                                     >  1:1244789/1‑101 (MQ=255)
 aTTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cc                                                                                                    <  2:2637047/101‑1 (MQ=255)
  ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                   >  2:1643810/1‑101 (MQ=255)
  ttCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccc                                                                                                   <  2:1707214/101‑1 (MQ=255)
   tCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.cccc                                                                                                  <  1:2034599/101‑1 (MQ=255)
    ccTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.ccccc                                                                                                 >  2:4593449/1‑101 (MQ=255)
          tCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCg                                                                                           <  1:519145/101‑1 (MQ=255)
            cAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGAc                                                                                         >  2:2505492/1‑101 (MQ=255)
               tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACggg                                                                                      >  2:3497630/1‑101 (MQ=255)
               tttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCACCCTG.CCCCCCCCCCgcccgg                                                                                      >  2:3391615/1‑96 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCgggg                                                                                     >  1:4206203/1‑100 (MQ=255)
                ttCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGa                                                                                     >  1:1179113/1‑101 (MQ=255)
                ttCTGTATGGGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGa                                                                                     <  1:3551734/101‑1 (MQ=255)
                 tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGag                                                                                    >  2:3790331/1‑101 (MQ=255)
                 tCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGag                                                                                    >  1:2918351/1‑101 (MQ=255)
                  cTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGggg                                                                                   >  2:2146191/1‑101 (MQ=255)
                    gTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGggggc                                                                                 >  1:1691505/1‑101 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagggcg                                                                                >  1:2986536/1‑100 (MQ=255)
                     tATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGagggca                                                                                <  1:1464221/101‑1 (MQ=255)
                      tttggAACCGGGGCTTACGCCTGGGGCTGGGGGGGGGCAATGCCCCGCACGGGCTTTTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGACGGGagggca                                                                                <  2:2340918/97‑1 (MQ=255)
                      aTGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGggggccg                                                                               >  2:515768/1‑99 (MQ=255)
                       tgtgAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCCACGGGgggggcgg                                                                              >  2:2753754/1‑97 (MQ=255)
                          taaCCGGGGCTTAGGGCTGGGGGTGGGGGGGGGCCATCGCCCCCAACGGCTTGTTAAGGCGCGGGCGCATCCCT.CCCCCCCCCCGACGGGAGGGCAGGGCt                                                                           <  2:1656725/100‑1 (MQ=255)
                          gAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGggggcggggcg                                                                           >  1:3175983/1‑100 (MQ=255)
                           aaCCGGGGCTTACCGCTGTGGCTGGGGTGGGGCAATGCCCCGCGCGGGTTTGTGAAGGCGCTGGCGCATCCTG.GCCCCCCCCCGACGGGAGGGCAGGGCTg                                                                          <  2:3086220/101‑1 (MQ=255)
                              cGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGtt                                                                       >  1:417948/1‑101 (MQ=255)
                               ggggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGttt                                                                      <  2:417911/101‑1 (MQ=255)
                                gggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGGTTc                                                                     >  2:4329633/1‑101 (MQ=255)
                                gggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                     >  1:2224864/1‑101 (MQ=255)
                                gggCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTc                                                                     <  2:1215009/101‑1 (MQ=255)
                                 ggCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCg                                                                    >  1:3869684/1‑101 (MQ=255)
                                 ggCTTACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTC.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCg                                                                    <  1:3391868/101‑1 (MQ=255)
                                   cTTACGCCTGGGGCTGCGGGGGGGCAATGCCCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCggg                                                                  <  1:3228455/101‑1 (MQ=255)
                                    ttgccccggtgGCTGGGGTGGGCCAATGACCCGCACTGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggc                                                                 <  2:4505441/93‑1 (MQ=255)
                                     tACGCCTGTGGCTGCGGGGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcg                                                                <  2:1967261/101‑1 (MQ=255)
                                      aCGCCTGTGGCTGCGGTGGGGCAATGCCCCGCACGGGCTTGTGAAGGCGCTGCGGCCTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGggcgg                                                               <  1:1159158/101‑1 (MQ=255)
                                      aCGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGGGGGCAGGGCTGGTTTCGggcgg                                                               >  1:3729/1‑101 (MQ=255)
                                        gCCTGTGGCGGCGGGGGGGCAATGACCCGCCCGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCa                                                             <  1:1483599/101‑1 (MQ=255)
                                         ccTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTGGGGGGGCaa                                                            >  1:2733672/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAg                                                           >  2:2801584/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAg                                                           >  2:1876898/1‑101 (MQ=255)
                                          cTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAg                                                           <  1:3647513/101‑1 (MQ=255)
                                           tgtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCGGGGCGGGTTTCGGGCGGCAAgg                                                          >  2:1486895/1‑101 (MQ=255)
                                            gtgGCTGGGGTGGGGCAATGACCCGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgc                                                         <  2:585653/101‑1 (MQ=255)
                                            gtgGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgc                                                         <  2:3117949/101‑1 (MQ=255)
                                              ggCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGGAGGGCGGGTTTCGgggggggaggggc                                                       >  1:4299951/1‑93 (MQ=255)
                                              ggCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGgcgc                                                       >  2:65183/1‑101 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      >  1:3166278/1‑101 (MQ=255)
                                               gCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGcc                                                      >  1:1958495/1‑101 (MQ=255)
                                                 tGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAGGGGCCcg                                                    >  1:878186/1‑101 (MQ=255)
                                                  ggggTGGGGGAATGGCCCGGACGGGGTTGGGAAGGGCGTGGCGCCTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                   <  1:1883501/99‑1 (MQ=255)
                                                  gCGGTGGGGCAATGACCCGCCCGGGCTTGTGAAGGCGCTGCGGCATCCTGCCCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcg                                                    <  1:2554743/101‑1 (MQ=255)
                                                  gCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgc                                                   <  1:2484670/101‑1 (MQ=255)
                                                   gcggggggAATTGCCCCGCCCGGGCTTGGGAAGGGCCTGCGGCATCCTT.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                  <  1:4532110/97‑1 (MQ=255)
                                                   cGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcg                                                  <  2:1032864/101‑1 (MQ=255)
                                                      tGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcg                                               <  1:3568916/101‑1 (MQ=255)
                                                        gggCAATTGCCCCCCCGGGCTTGGGGAAGCCCTGGGGCCTCCCG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCcgcggcgcg                                             <  2:1325798/101‑1 (MQ=255)
                                                           gaaTGGCCCGCACGGGCTTGTGAAGGCCCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGcc                                          <  2:2923954/100‑1 (MQ=255)
                                                               gACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGCCGGGGGGGCCGGGGTGGGTTCCGGCGGGAAGGCGCCCCCCGCGCCGccccgc                                      >  2:98025/1‑97 (MQ=255)
                                                                 cccGCACGGGCTTGTTAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                    <  2:3249193/101‑1 (MQ=255)
                                                                 cccGCACGGGCTTGGGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGcat                                    <  1:920138/101‑3 (MQ=255)
                                                                 cacgacgcggCTTGTGAAGGGGGTTCGTCGCGCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCt                                    <  2:4557276/93‑1 (MQ=255)
                                                                   cGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGGGGTCAGGCGCCCGCGGCGCGGGCGGGccccc                                  >  1:436901/1‑98 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGGAAGGCGCCCGCGGCGCGGCCCGGCCCGCt                                 >  1:1835130/1‑101 (MQ=255)
                                                                    gCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCt                                 >  2:3193610/1‑101 (MQ=255)
                                                                     cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  1:65189/101‑1 (MQ=255)
                                                                     cACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCtt                                <  2:1958360/101‑1 (MQ=255)
                                                                      gggggCTTGGTGAGGCCCTGCCGCAACCTG.CCCCCCCCCCGGCGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  2:2847901/99‑1 (MQ=255)
                                                                      aCGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTa                               <  1:4561544/101‑1 (MQ=255)
                                                                        gggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACg                             >  2:217542/1‑101 (MQ=255)
                                                                         ggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGGCGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCtttccc                            >  2:396458/1‑97 (MQ=255)
                                                                         ggCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGGGGGCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCCGGCCTGCTTACGc                            >  1:586764/1‑101 (MQ=255)
                                                                          gCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGcc                           <  1:2600727/101‑1 (MQ=255)
                                                                           cTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGCCAGGGCTGGTTTCGGGCGGCAAGGGGCCCGCGGCGCGGCCGCGCCTGCTTACGccc                          >  1:4632181/1‑100 (MQ=255)
                                                                           cTTGTGAAGGCGCTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         >  2:2961445/1‑101 (MQ=255)
                                                                            ttGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCCCGGCGCGGCCGGGCCTGCTTACGCCTg                         >  2:3641102/1‑101 (MQ=255)
                                                                            ttGGGAGGGGGCTGCGGCCCCTTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTg                         <  2:679557/101‑1 (MQ=255)
                                                                             ggggAAGGCGGTGGGCCCCCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGc                        <  1:838148/98‑1 (MQ=255)
                                                                               tgAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCCGCTTACCCCTGCAg                      >  1:4121233/1‑101 (MQ=255)
                                                                                  aGGCGCTGCGGCACCCCG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCgg                   <  1:2663649/101‑1 (MQ=255)
                                                                                   ggCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                  <  1:2801800/101‑1 (MQ=255)
                                                                                   ggCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGc                  <  2:2566588/101‑1 (MQ=255)
                                                                                    gcgcgtcGGCATCCGG.CCCCCCCCCCGACGNGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                 <  1:1519062/95‑1 (MQ=255)
                                                                                    gcgcTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                 <  2:3729274/101‑1 (MQ=255)
                                                                                    gcgcTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGcc                 <  1:717328/101‑1 (MQ=255)
                                                                                     cggtGCGGCCTCCCG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCg                <  1:3345023/98‑1 (MQ=255)
                                                                                      gcTGCGGCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCCGCGGCCggg              >  1:2674774/1‑100 (MQ=255)
                                                                                       cTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCggg              >  2:343489/1‑100 (MQ=255)
                                                                                         gctgCATCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCg           >  1:3832164/4‑101 (MQ=255)
                                                                                          cGGCCTCCTG..CCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCga          <  1:1990830/101‑1 (MQ=255)
                                                                                           ggCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGCCCgg          >  1:3763542/1‑100 (MQ=255)
                                                                                           ggCACCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCga          <  1:46970/101‑1 (MQ=255)
                                                                                             cATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCgaga        <  2:3547768/101‑1 (MQ=255)
                                                                                             cATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCCGCCGTGCCTGCTTACGCCCGGCGCGGCCGGACCgcga        >  2:402515/1‑101 (MQ=255)
                                                                                              aTCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAt       >  1:1307901/1‑101 (MQ=255)
                                                                                                ccTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGAttt     <  2:1154120/101‑1 (MQ=255)
                                                                                                 cTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGGGGCAAGGCGCCCGCGGCGCGGCCGTGCCCGCTTACGCCCGCAGCGGCCGGGCCGGGATTTc    >  2:3341942/1‑101 (MQ=255)
                                                                                                 cTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGGGCCCGCTTACGCCTGCAGCGGCCCGCCCgcggcttc    >  2:3930404/1‑94 (MQ=255)
                                                                                                     |                                                                                                   
CATTCCTGCCTCCAGTTTCTGTATGTGAACCGGGGCTTACGCCTGTGGCTGCGGTGGGGCAATGACCCGCACGGGCTTGTGAAGGCGCTGCGGCATCCTG.CCCCCCCCCCGACGGGAGGGCAGGGCTGGTTTCGGGCGGCAAGGCGCCCGCGGCGCGGCCGTGCCTGCTTACGCCTGCAGCGGCCGGACCGAGATTTCGC  >  NC_014034/568702‑568901

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG