| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_014034 | 1,640,005 | +GGCA | 100% | intergenic (+85/‑60) | mccB → / → mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
| Read alignment evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | change | freq | score | reads | annotation | genes | product | ||
| * | NC_014034 | 1,640,005 | 1 | .→G | 100.0% | 15.2 | 74 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
| Reads supporting (aligned to +/- strand): new base (66/1): ref base (2/5): total (68/6) | ||||||||||
| Fisher's exact test strand distribution p-value = 7.63e-06 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.26e-04 | ||||||||||
| * | NC_014034 | 1,640,005 | 2 | .→G | 100.0% | 15.2 | 73 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
| Reads supporting (aligned to +/- strand): new base (65/1): ref base (2/5): total (67/6) | ||||||||||
| Fisher's exact test strand distribution p-value = 8.18e-06 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.11e-03 | ||||||||||
| * | NC_014034 | 1,640,005 | 3 | .→C | 100.0% | 14.9 | 73 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
| Reads supporting (aligned to +/- strand): new base (65/0): ref base (2/5): total (67/6) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.50e-06 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.72e-02 | ||||||||||
| * | NC_014034 | 1,640,005 | 4 | .→A | 100.0% | 15.9 | 73 | intergenic (+85/‑60) | mccB/mccA | methylcrotonoyl‑CoA carboxylase subunit beta/methylcrotonoyl‑CoA carboxylase subunit alpha |
| Reads supporting (aligned to +/- strand): new base (65/0): ref base (2/5): total (67/6) | ||||||||||
| Fisher's exact test strand distribution p-value = 1.50e-06 | ||||||||||
| Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.49e-03 | ||||||||||
Only 100 of 105 total aligned reads displayed.
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGG.....G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCC > NC_014034/1639905‑1640088
||||
gATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGC.CAAAAGAAAACCCCCCCCTT.CATC.TT.GCCCC.CAAAAT.CC.CCCGGGGGC.C..gggg > 1:3276442/1‑101 (MQ=255)
gATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg < 1:3033990/101‑1 (MQ=255)
gATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg < 1:275683/101‑1 (MQ=255)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGC.AAAAGAAAAAACACACCTTT.TATC.TT.TCCCC.CAATAT.CC.CCGGGTGCC.C..gggg.....gg > 2:1046555/1‑100 (MQ=255)
tCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.GAATAT.CC.CGGGGGGCC.C..gggg.....gg > 2:1355834/1‑100 (MQ=255)
cTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggc < 1:1949940/101‑3 (MQ=255)
ttCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggca < 2:2133467/101‑4 (MQ=255)
gatctgATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggca < 2:2771009/96‑4 (MQ=255)
tCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggcag < 2:369204/101‑5 (MQ=255)
ccGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggcagc < 1:1983164/101‑6 (MQ=255)
cGGATGTGACTTTCGATGCCTCCGGCGGGGGTATTTTGGGG.AAGAAGAAAACCATCCCCTT.CATC.TT.TCCCC.CAAAAA.AC.CCGGGGGCC.C..gggg.....ggcaggg > 2:3374372/1‑95 (MQ=255)
cGGATGTGACTTTCGATGCCTCCGGCGGGGAGATTTGGGGG.GAAACAAAACCCAACCCTTT.CATC.TT.GCCCC.AAAAAA.TC.CCCGGGGCC.C..ggcg.....gggggag > 1:2877610/1‑95 (MQ=255)
ggATGTGACTTTCGATGCCTCCGGCGGGGGGATTTTGGGG.AAGATGAAAACCATCCCCTT.CATCTTT.CCCCC.AAAATT.CC.CGGGGGGCC.C..GGGG.....g.....c.ggcgc > 1:2564080/1‑97 (MQ=255)
ggATGTGACTTTCGATGCCTCCGGCGGGGGGAATTTGGGG.AAGAAGAAAACCATCCCCTT.CATC.TTGGCCCC.AATAAT.CC.GGGGGGTCC.C..gggg.....ggagcgg > 2:484986/1‑95 (MQ=255)
gATGTGACTTTCGATGCCTCCGGCGGGGGTATTTTGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.TCCCC.CAATAT.CC.CGGGGGGCC.C..gggg.....gggaagagc > 1:2771207/1‑93 (MQ=37)
gATGTGACTTTCGATGCCTCCGGCGGGGATATTTTGGGG.AAGATGAAAACCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CCGGGGGCC.C..gggg.....ggcggcgcc > 2:1973665/1‑93 (MQ=37)
gATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..gggg.....ggcagcgcc < 2:408430/101‑9 (MQ=255)
aTGTGACTTTCGATGCCTCCGGCGGGGAAATTTGGGGC.AAGATGAAAACCATCCCTTT.CATC.TT.GCCCC.CAATATACC.CGGGGGGCC.G..GGGG.....g.....cagggccc > 1:4482631/1‑94 (MQ=25)
tgtgACTTTCGATGCCTCCGGCGGGGGTATTTGGGGG.AAGATGAAAACCCTCCCTTT.CATC.TT.GCCCC.CAATAT.CC.CCGGGGGCC.CCGGGGG.....G.....C.G.....c....gcccc > 1:2993086/1‑101 (MQ=25)
gtgACTTTCGATGCCTCCGGCGGGGCTATTTGGGGG.AAGGTGGAACCCCACCCTTT.AATC.TT.TCCCCAAAACAT.CCATGGGGGCCC.C..GGGG.....G.....A.G.....c....gccccc > 2:1560735/1‑100 (MQ=255)
gACTTTCGATGCCTCCGGCGGGGGTATTTGGGGG.AAGATGAAAACCATCCCTTT.CATC.TT.GCCCC.CAAAAT.CC.CCGGGGGCC.C..GGGG.....g.....g.g.....cgcgccccccc > 2:2648150/1‑91 (MQ=25)
tCCGGCGGGGATATTTGGGGC.AAGATGAAAACCATCCCTTT.TATC.TT.GCCCC.AAATAT.CC.CCGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCCCCTCCGcccc > 2:2949799/1‑99 (MQ=18)
ccGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCCCCACCTCCGCCCCg > 2:1849659/1‑101 (MQ=18)
cggGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGc > 2:4215023/1‑101 (MQ=25)
ggggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCCCTCCCGCCACGGGGcg > 2:1886278/1‑100 (MQ=18)
ggggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCa > 2:3166966/1‑101 (MQ=25)
ggggAT.TTTTGGGC.AAAGTTAACCCCCCCCCTTT.CTCT.TT.CCCCC.AAATTA.CC.GGGGGGGCC.G..GGGG.....G.....G.GCCCCCC....CCCCCGCCACTTCCGCCACGGGGCa < 1:1226654/101‑1 (MQ=255)
gggattatTGGGCGA.AAGAT.AAACCCCACCCTTT.TCTT.TT.GCCCC.CAAATC.CC.CGGGGGGCC.G..GGGG.....GGGCCCC.C.....C....CCCCCGCCACTTCCGCCACGGGGCa < 2:1272302/96‑1 (MQ=255)
ggggtttttGGGGAAAAGATAAAACCCACCCCTTT.CATC.TT.GCCCA.AAATA..CC.CGGGGGGCC.C..GGGGGGGCAG.....C.C.....C....CCCCCGCCACTTCCGCCACGGGGCa < 2:1528318/95‑1 (MQ=9)
gggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAg > 2:2360976/1‑101 (MQ=25)
gggATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCCCCACTTCCGCCACGGGGCAg > 2:869358/1‑101 (MQ=21)
atatTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAgcgc > 2:652466/1‑101 (MQ=25)
ttttgggggA.AAGATAACCCCCCCCCTTTT.CATC.TT.CCCCA.AAATCC.CC.GGGGGGGCCGG..GGGG.....G.....C.GCCC..C....CCCCCGCCACTTCCGCCACGGGGCAGCGcc < 1:2865124/96‑1 (MQ=255)
tatTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGcc > 2:4414143/1‑101 (MQ=25)
tatTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGACACGGGGCAgcgac > 2:497705/1‑99 (MQ=21)
atTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGccc > 2:30930/1‑101 (MQ=25)
atTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGccc > 2:2724658/1‑101 (MQ=25)
atTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGG.....G.....G.G.....CGGCGCCCCCGCCCCCTCCCCCCCCGGGCAGCGccc > 2:1595201/1‑101 (MQ=18)
tttGGGGA.CAAAAGAAACCCCTCCCTTT.TGTT.CT.CCCCC.AAACTC.CC.GGGGGGGCG.CGGGGGG.....G.....C.GCCC..C....CCCCCGCCACTTCCGCCACGGGGCAGCGccc < 2:4482336/101‑1 (MQ=255)
ttGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCg > 2:2452655/1‑101 (MQ=25)
tGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg > 2:1843511/1‑101 (MQ=25)
tGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg > 2:2578617/1‑101 (MQ=25)
tGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCgg > 2:3569181/1‑101 (MQ=25)
gacc.AAGATCAACCCCCCCCCTTC.CATC..T.CCCCC.AAATTC.CC.CGGGGGGCC.C..GGGG.....GGCCCCC.C.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGcc < 2:3473768/98‑1 (MQ=255)
c.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGtt > 2:255102/1‑101 (MQ=25)
gcaaaagAAACCCCCCCCTTTCCATC.TC.CCCCC.AAATA..CC.GGGGGGGCC.C..GGGG.....GGGC..C.GCC...C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGt < 1:1355929/95‑1 (MQ=255)
aaGATAAACCCCATCCCTTT.CA.C.TT.TCCCC.AAAATT.CC.CGGGGGGGC.C..GGGG.....GGGCACC.C.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTg < 2:1326313/101‑1 (MQ=255)
aGATGAACCCCACCCTTTT.CATC.TG.CCCCC.AAATAC.CC.CGGGGGGCC.G..GGGG.....G.....CAGCC...C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGAc < 1:4201770/101‑1 (MQ=255)
aGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa > 1:3003873/1‑101 (MQ=25)
aGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa > 1:4239857/1‑101 (MQ=25)
aGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGa > 1:2492883/1‑101 (MQ=25)
gATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGAc > 1:198338/1‑101 (MQ=25)
aTGAAACCCATCCCTTT.CATC.AT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACg > 1:2645942/1‑101 (MQ=25)
gAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa > 2:3752605/1‑101 (MQ=25)
aacccccccccTTT.TCTC.CT.GCCCC.AAAGTC.CC.CGGGGGGCC.CGGGGGG.....G.....C.GCCC..C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGaa < 2:2823794/93‑1 (MQ=255)
aaaCCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCCGCGCCCCCGCCGTTGACGAAg > 2:1581271/1‑101 (MQ=21)
aaaCCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAg > 2:190887/1‑101 (MQ=25)
aaCCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg > 1:3136026/1‑101 (MQ=25)
aaCCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAgg > 2:4326659/1‑101 (MQ=25)
cccccccctttt.CTTC.TT..CCCC.AAAATC.CC.CGGGGGGCC.C..GGGG.....GGGC..C.GCC...C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGga < 1:3067678/92‑1 (MQ=255)
cccATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCCGCGCCCCGGCCGTTGACGAAgggg > 2:826190/1‑99 (MQ=21)
aTCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc > 1:2122948/1‑101 (MQ=25)
aTCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGAcc > 2:2914303/1‑101 (MQ=25)
tCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg > 1:83676/1‑101 (MQ=21)
tCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcg > 1:3846642/1‑101 (MQ=25)
ccTTT.CCTT.TT.GCCCC.AAAATC.CC.CGGGGGGCC.G..GGGG.....GGCCCCC.C.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACtgc < 2:1232427/101‑1 (MQ=255)
ccTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGCAGGAGACcgcg > 2:1603274/1‑101 (MQ=21)
ccTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcgcg > 1:1338230/1‑101 (MQ=25)
ccTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcgcg > 1:2002142/1‑101 (MQ=25)
ccTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACcgcg > 1:3621634/1‑101 (MQ=25)
ccTTT.CACC.GT.CCCCC.AAATTC.CC.CGGGGGGGC.C..GGGG.....GGGCCCC.C.....C....CCCCCGCCACTTCCGCCACGGGACAGCGCCCCGGCCGTTGACGAAGGAGACcgc < 2:1835342/101‑1 (MQ=255)
cTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CGCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGa > 2:4028967/1‑101 (MQ=25)
ttt.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt > 1:444767/1‑101 (MQ=25)
ttcccc.TT.CCCCC.AAATGC.CC.CGGGGGGCC.C..GGGG.....GGC...C.GCCC..C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGAt < 2:3373699/96‑1 (MQ=255)
t.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 2:3346597/1‑101 (MQ=25)
t.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 1:3649654/1‑101 (MQ=25)
t.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 2:3558206/1‑101 (MQ=25)
t.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGt > 2:3796993/1‑101 (MQ=25)
cATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 1:3058043/1‑101 (MQ=25)
cATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 2:4469830/1‑101 (MQ=25)
cATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGtt > 2:3897908/1‑101 (MQ=25)
aTC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt > 1:1349631/1‑101 (MQ=25)
aTC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCCCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGttt > 1:1897689/1‑101 (MQ=25)
t.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCaaa > 1:1549628/1‑101 (MQ=25)
cccc.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAg > 1:2541920/1‑101 (MQ=25)
cccc.AAATAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCAGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAg > 2:3654005/1‑101 (MQ=25)
aaaTAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATcc > 1:1182454/1‑101 (MQ=25)
aaTAT.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCt > 2:2849596/1‑101 (MQ=25)
atat.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg > 2:3511336/1‑101 (MQ=37)
atat.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg > 2:449998/1‑101 (MQ=37)
atat.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTg > 2:645818/1‑101 (MQ=37)
tat.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGa > 1:3437014/1‑101 (MQ=37)
at.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt > 2:2788189/1‑101 (MQ=37)
at.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGAt > 1:1884789/1‑101 (MQ=37)
t.CC.CGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGAGGAAGGAGACCGCGATGTTTCAAAAGATCCTGATc > 1:4002554/1‑101 (MQ=37)
cc.cGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCg > 1:3028055/1‑101 (MQ=37)
c.cGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGc > 1:1907525/1‑101 (MQ=37)
cGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTNAAAAGATCCTGATCGcc > 2:892503/1‑101 (MQ=37)
cGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc > 2:4353945/1‑101 (MQ=37)
cGGGGGGCC.C..GGGGGGCA.G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGcc > 1:4382835/1‑101 (MQ=37)
||||
GATCTTCCGGATGTGACTTTCGATGCCTCCGGCGGGGATATTTGGGGC.AAGATGAAACCCATCCCTTT.CATC.TT.GCCCC.AAATAT.CC.CGGGGGGCC.C..GGGG.....G.....C.G.....C....CCCCCGCCACTTCCGCCACGGGGCAGCGCCCCGGCCGTTGACGAAGGAGACCGCGATGTTTCAAAAGATCCTGATCGCC > NC_014034/1639905‑1640088
Base quality scores:
ATCG
< 3 ≤
ATCG
< 18 ≤
ATCG
< 27 ≤
ATCG
< 33 ≤
ATCG
< 41 ≤
ATCG
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