Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,276 +GT 100% coding (101/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2761.→G100.0% 636.8 174coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (90/82):  ref base (1/1):  total (91/83)
*NC_0140342,177,2762.→T100.0% 666.2 174coding (101/990 nt)rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (90/82):  ref base (1/1):  total (91/83)

Only 100 of 198 total aligned reads displayed.

ACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGT..TG..G..T..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177180‑2177354
                                                                                                     ||                                                                                     
acaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..t                                                                                   <  1:1580219/101‑1 (MQ=255)
  aTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgt                                                                                 >  2:222138/1‑99 (MQ=255)
     gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcgg                                                                              <  1:1123047/101‑6 (MQ=255)
     gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcgg                                                                              >  1:1380398/1‑96 (MQ=255)
     gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcgg                                                                              >  1:4452599/1‑96 (MQ=255)
     gCCATCCATCTTGCCCGGATAGGTCAAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcgg                                                                              <  2:2272324/101‑6 (MQ=255)
      ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcggt                                                                             <  1:549359/101‑7 (MQ=255)
       cATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcggtc                                                                            >  1:3682720/1‑94 (MQ=255)
        aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCGCGGTCGCCTTTGCCGAAGCACCTTCCTTCCTCTTGACGAAAAAGGGGT..Tg..g..tgtcggtct                                                                           <  1:4148473/101‑9 (MQ=255)
         tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..Tg..g..tgtcggtctt                                                                          >  2:3475521/1‑92 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.gg                                                                       >  1:1564153/1‑100 (MQ=255)
           gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.gg                                                                       <  2:3499269/101‑2 (MQ=255)
              cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtga                                                                    <  1:3297993/101‑5 (MQ=255)
               ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgat                                                                   >  2:3792214/1‑96 (MQ=255)
               ttGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgat                                                                   >  1:3523979/1‑96 (MQ=255)
                tGCCCGCATAGGTCAAGAGCCCCAGCCCCATCGCGGTCCCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgatc                                                                  <  1:4054452/101‑7 (MQ=255)
                 gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgatca                                                                 <  1:3172036/101‑8 (MQ=255)
                  cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgatcag                                                                <  2:857954/101‑9 (MQ=255)
                  cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgatcag                                                                <  1:3085174/101‑9 (MQ=255)
                    cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTt.ggtgatcagac                                                              >  1:3210523/1‑91 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAgg                                                            <  2:26867/101‑1 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAgg                                                            >  1:3565271/1‑101 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAgg                                                            <  2:2097821/101‑1 (MQ=255)
                       aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAgg                                                            >  2:1940203/1‑101 (MQ=255)
                        tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGc                                                           <  1:1134447/101‑1 (MQ=255)
                         aGGTCGAGAGCCCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGcc                                                          <  1:2851308/101‑1 (MQ=255)
                         aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGcc                                                          <  1:3621927/101‑1 (MQ=255)
                           gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGcccc                                                        <  2:3917758/101‑1 (MQ=255)
                              gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                     >  2:1288617/1‑101 (MQ=255)
                              gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGt                                                     >  2:893827/1‑101 (MQ=255)
                               agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGtt                                                    >  1:1651151/4‑101 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..TGGTCTTGGTGATCAGACTGGCCCCGGttt                                                   <  2:2039335/99‑1 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   <  2:3580360/99‑1 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   >  2:1816124/3‑101 (MQ=255)
                                gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGttt                                                   >  1:1344486/3‑101 (MQ=255)
                                  gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCg                                                 <  1:2376930/101‑1 (MQ=255)
                                   cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                >  2:407594/1‑101 (MQ=255)
                                   cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCgg                                                <  1:4501086/101‑1 (MQ=255)
                                    aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGc                                               <  2:2257341/101‑1 (MQ=255)
                                     ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGcc                                              >  1:3312310/1‑101 (MQ=255)
                                       aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.a                                           <  1:222162/101‑1 (MQ=255)
                                         ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aaa                                         >  2:3290119/1‑99 (MQ=255)
                                          cccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagc                                        >  2:352001/1‑98 (MQ=255)
                                           ccATCTCGGTCGCCTTTGCCGAAGCTCCTTCCTTCATCTTGACGAAAAAGGGGTTGTG..G..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  2:1311785/101‑5 (MQ=255)
                                           ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagcc                                       <  2:1182897/101‑5 (MQ=255)
                                            cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCG.aagccg                                      <  1:1053381/101‑6 (MQ=255)
                                             aTCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAgccgc                                      >  2:2501397/1‑101 (MQ=255)
                                               ctcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGccc                                    >  2:3317274/1‑101 (MQ=255)
                                                tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   >  1:3335861/1‑101 (MQ=255)
                                                tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                   <  1:3505652/101‑1 (MQ=255)
                                                 cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                  >  2:1395632/1‑101 (MQ=255)
                                                 cGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCg                                  >  1:4286492/1‑101 (MQ=255)
                                                   gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                >  2:83239/1‑101 (MQ=255)
                                                   gTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGc                                >  1:4429419/1‑101 (MQ=255)
                                                    tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                               <  1:1512349/101‑1 (MQ=255)
                                                    tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                               >  1:2713049/1‑101 (MQ=255)
                                                     cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGccg                              >  2:4156338/1‑100 (MQ=255)
                                                     cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                              >  2:3549936/1‑101 (MQ=255)
                                                      gCCTTTTCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                             <  1:775533/101‑1 (MQ=255)
                                                       ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            <  1:1870166/101‑1 (MQ=255)
                                                       ccTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgc                            >  2:580064/1‑101 (MQ=255)
                                                         tttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAgcgc                          <  2:3155/101‑1 (MQ=255)
                                                          ttGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGcgcg                         <  2:1427927/101‑1 (MQ=255)
                                                           tGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                        <  1:2529128/101‑1 (MQ=255)
                                                            gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       >  2:620292/1‑101 (MQ=255)
                                                            gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       <  1:72483/101‑1 (MQ=255)
                                                            gCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                       <  1:2441702/101‑1 (MQ=255)
                                                             ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      >  2:1506157/1‑101 (MQ=255)
                                                             ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      <  2:107625/101‑1 (MQ=255)
                                                             ccGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaa                      <  1:666329/101‑1 (MQ=255)
                                                              cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     >  2:1586815/1‑101 (MQ=255)
                                                              cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     >  2:2353656/1‑101 (MQ=255)
                                                              cGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                     >  2:2390484/1‑101 (MQ=255)
                                                               gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACCGGCCCCGGCTTCGGCCGAAGCCGGCCCGGCCAGCGCCGacaa                    >  1:128843/1‑101 (MQ=255)
                                                               gAAGCACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                    <  2:4567813/101‑1 (MQ=255)
                                                                 aggaCCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                  <  1:3849646/98‑1 (MQ=255)
                                                                   cACCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGcc                <  1:3480781/101‑1 (MQ=255)
                                                                    cttccgatctaTCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               <  1:3382495/91‑1 (MQ=255)
                                                                    aCCTTCCTTCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               <  2:535089/101‑1 (MQ=255)
                                                                    aCCTTCCTTCATCTTAACGAAAAAGGGGT..TG..GTCT..CGGTCTTGGTGATCAAACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa               <  1:3973849/101‑1 (MQ=255)
                                                                     ccttccttCATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g             <  1:486626/101‑1 (MQ=255)
                                                                      cttccttcATCTTGGCGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  1:2145410/101‑1 (MQ=255)
                                                                      cttccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            >  2:4638462/1‑101 (MQ=255)
                                                                      cttccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg            <  2:4380081/101‑1 (MQ=255)
                                                                       ttccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           >  1:4223900/1‑100 (MQ=255)
                                                                       ttccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.ggg           >  1:951329/1‑100 (MQ=255)
                                                                        tccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          <  2:640831/101‑3 (MQ=255)
                                                                        tccttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc          >  2:3989549/1‑99 (MQ=255)
                                                                          cttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag        <  1:4601486/101‑5 (MQ=255)
                                                                          cttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag        >  1:3025176/1‑97 (MQ=255)
                                                                           ttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:1039340/1‑96 (MQ=255)
                                                                           ttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  2:3967470/1‑96 (MQ=255)
                                                                           ttcATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga       >  1:434106/1‑96 (MQ=255)
                                                                             cATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  2:2474680/1‑101 (MQ=255)
                                                                             cATCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg      >  1:1273251/1‑101 (MQ=255)
                                                                              aTCTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCaagatcggaag     >  2:3382245/1‑91 (MQ=255)
                                                                               tCTTGACGAAAAAGGGGT..TG..G..TGGCGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcc    >  2:1827423/1‑101 (MQ=255)
                                                                                cTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc   >  2:4497451/1‑101 (MQ=255)
                                                                                cTTGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGccc   >  1:2881723/1‑101 (MQ=255)
                                                                                 ttGACGAAAAAGGGGT..TGGTG..T..CGGTCTTGGTGATCAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGcccc  <  1:3265855/101‑1 (MQ=255)
                                                                                                     ||                                                                                     
ACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGT..TG..G..T..CGGTCTT.GTGTACAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGGCCCC  >  NC_014034/2177180‑2177354

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG