Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,494,076 +C 13.1% intergenic (+124/‑118) RCAP_rcc02319 → / → RCAP_rcc02320 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,494,0760.→C13.1% 35.4 0.0 ‑0.4106intergenic (+124/‑118)RCAP_rcc02319/RCAP_rcc02320hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (7/4):  ref base (55/37):  total (64/42)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.85e-01

Only 100 of 121 total aligned reads displayed.

AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170
                                                                                                                                                                                                          
aaGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCa                                                                                                  <  1:814009/101‑1 (MQ=18)
 aGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCag                                                                                                 <  2:1264520/101‑2 (MQ=18)
  gCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGGCTTTCATCTTGCCCGAAATGTCAACGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCagc                                                                                                >  1:3989609/1‑99 (MQ=17)
    ggACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCagcgc                                                                                              >  1:798133/1‑97 (MQ=17)
      aCTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGG..GAAG.CCAG.AGGGGGGC.AGCG.ccc                                                                                          >  2:4255159/1‑101 (MQ=17)
       cTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.c                                                                                        >  2:486759/1‑101 (MQ=18)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCG.ccc.cc                                                                                       <  2:1714876/101‑1 (MQ=17)
        tCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.cc                                                                                       >  1:2173295/1‑101 (MQ=18)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                                                     <  2:2243612/101‑3 (MQ=17)
          cccccGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccct                                                                                     <  1:2160141/101‑3 (MQ=17)
           ccccGGGATATCTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.ccc.ccctg                                                                                    >  1:2729047/1‑98 (MQ=17)
              cGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GACAGAGGGGGGC.AGCG.ccc.ccctgacc                                                                                 >  1:1814513/1‑95 (MQ=17)
               gggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCAC.CCC.CCTGcccc                                                                                 >  2:344576/1‑101 (MQ=17)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                                                >  2:4133702/1‑101 (MQ=2)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                                                <  1:2338829/101‑1 (MQ=0)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                                                >  2:1903843/1‑101 (MQ=0)
                ggATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcg                                                                                >  1:1282178/1‑101 (MQ=2)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                                                >  2:4256564/1‑101 (MQ=17)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                                                <  1:1726617/101‑1 (MQ=17)
                ggATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcg                                                                                >  1:991533/1‑101 (MQ=18)
                 gATATTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgc                                                                               >  2:4290240/1‑100 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                                              >  1:3869648/1‑99 (MQ=0)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                                              <  2:2444885/101‑3 (MQ=2)
                  atatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgcc                                                                              <  1:1127028/101‑3 (MQ=2)
                  atatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCcggc                                                                              >  2:477874/1‑101 (MQ=17)
                   tatTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgccg                                                                             <  2:2091493/101‑1 (MQ=2)
                   tatTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGACCcggcg                                                                             >  2:1611164/1‑101 (MQ=17)
                    atTTGCGGCAAGATGAAGGGGCATG.ATTTCATCTTGCCCGAAATATCCC.AGGGGTGAATTGGCCAA.AG.GCCA.AGAGGGGGCAGCG.CCC.CCTGCCCcgccgg                                                                            >  1:4479250/1‑101 (MQ=2)
                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGCAGCGG.CCC.CCGGCCCCGGCGGTc                                                                          >  2:714330/1‑101 (MQ=18)
                      ttGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTc                                                                          <  1:1467624/101‑1 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                                         <  2:1703597/101‑1 (MQ=17)
                       tGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTcc                                                                         >  1:3926664/1‑101 (MQ=18)
                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                                        >  1:1658069/1‑101 (MQ=17)
                        gcagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCg                                                                        >  1:4037011/1‑101 (MQ=18)
                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCC.CCCGCCCCCGCGGGcccgg                                                                      >  1:4092509/1‑98 (MQ=17)
                         cagcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGCGAA.TGGC..GAAG.CCAC.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGt                                                                       >  1:2256617/1‑101 (MQ=18)
                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAG.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                                                     >  2:294835/1‑101 (MQ=18)
                           gcaAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTgg                                                                     <  2:2629435/101‑1 (MQ=17)
                            caAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggc                                                                    >  2:2049065/1‑101 (MQ=18)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTggcgg                                                                  <  2:3823272/101‑1 (MQ=17)
                              aGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGG.AAGCGCCCC.CCTGCCCCGGCGGTCCGGggcgg                                                                  >  1:1331463/1‑101 (MQ=17)
                                 tGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGgcgc                                                               >  1:4604971/1‑101 (MQ=17)
                                   aaaCCGGATGCCTTTCATCTTCCCCGAAATGTCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCt                                                             <  2:3182635/101‑1 (MQ=17)
                                     ccgggctGCCTTTAATCTTG.CCAAAACTTGCCGGGGGGGGAAGGGGC..GAAG.CGGG.GGGGGGCCACCCC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTg                                                           <  2:2728849/95‑1 (MQ=255)
                                        ggATGCCTTTCATCTTTCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcg                                                        <  1:3085165/101‑1 (MQ=17)
                                         gATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgg                                                       >  1:1199947/1‑101 (MQ=17)
                                           tGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggc                                                     >  2:3668466/1‑101 (MQ=18)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  <  2:2173145/101‑1 (MQ=17)
                                              cTTTCATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGAcgggcggg                                                  >  1:4153737/1‑101 (MQ=18)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  1:2721423/101‑1 (MQ=17)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  1:402025/101‑1 (MQ=17)
                                               tttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCgggg                                                 <  2:3939830/101‑1 (MQ=17)
                                                ttCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCggggg                                                >  2:2317350/1‑101 (MQ=18)
                                                 tCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgc                                               <  2:3763269/101‑1 (MQ=18)
                                                  cATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCC.GCGGGGGCCAGCGCCCC.CCTGCCCCGCCGGTCCGTGGCGGGGCCTTGACGGGCggggggg                                              >  2:349165/1‑99 (MQ=12)
                                                  cATCTTGCCCGAAATATCCCGGGGGGGGAA.TGGG..GAAG.TCAA.GGGGGGGCAGGCC.CCC.CCTAGCCAGGGCGTCCGGGGCGGGCCCTGGAGGGGGggggggg                                              >  1:2574823/1‑99 (MQ=9)
                                                   aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                                             <  1:31269/101‑1 (MQ=18)
                                                   aTCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGgcgc                                             >  1:3214311/1‑101 (MQ=18)
                                                    tCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGcgcg                                            >  1:3698092/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGGGCgg                                           >  1:2097304/1‑101 (MQ=18)
                                                     cTTGCCCGAAATATCCCGGGGGGAGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGGGCCTTGCCGCGCTGGGGCGCgg                                           >  1:3664851/1‑101 (MQ=12)
                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGc                                         <  2:2943018/101‑1 (MQ=18)
                                                       tGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AA.GCCA.GAGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                        >  1:3546810/1‑101 (MQ=21)
                                                        gCCCGAAATATCCCGGGGGGTGGA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCa                                        >  2:1332826/1‑101 (MQ=18)
                                                          ccGAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAtt                                      <  1:2906827/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCagatc                                    >  2:2489016/1‑97 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  1:2256477/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGTGAA.TCGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    <  1:4255447/101‑1 (MQ=18)
                                                            gAAATATCCCGGGGGGGGAA.TGGC..GAAG.CCAG.AGGGGGGCATCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGTGGGCGTGGGCATTaa                                    >  2:449851/1‑101 (MQ=21)
                                                            gAAATATCCCGGGGGGGGAA.AGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTaa                                    >  2:2097177/1‑101 (MQ=18)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                <  2:798059/101‑1 (MQ=21)
                                                                tatCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGa                                >  2:2484677/1‑101 (MQ=21)
                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  2:14019/101‑1 (MQ=21)
                                                                  tCCCGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGaaa                              <  1:867912/101‑1 (MQ=21)
                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             <  2:4246277/101‑1 (MQ=21)
                                                                   cccGGGGGGTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAAt                             >  1:890800/1‑101 (MQ=21)
                                                                      ggggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCG.CCCACCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGc                          <  1:3245406/101‑1 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  1:2084434/1‑101 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         <  1:143055/101‑1 (MQ=25)
                                                                       gggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCa                         >  1:4286504/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAGCCAG..AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  2:39123/1‑101 (MQ=25)
                                                                        ggggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaa                        >  1:2897388/1‑101 (MQ=25)
                                                                         gggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCaaa                       >  1:454746/1‑100 (MQ=25)
                                                                          ggTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGACCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCAATAACCGAAATTGCAACa                      >  2:2854310/1‑101 (MQ=21)
                                                                           gTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     <  1:3579218/101‑1 (MQ=25)
                                                                           gTGAA.TGGC..GAAG.CCAG.AGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAg                     >  2:4632422/1‑101 (MQ=25)
                                                                                       gcgaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGAtgctgc              <  2:3794888/91‑1 (MQ=38)
                                                                                        cgaagccagaGGGGGGCATCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  1:4487127/92‑1 (MQ=255)
                                                                                        cgaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCg             <  1:4224228/92‑1 (MQ=38)
                                                                                         gaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  2:226709/9‑101 (MQ=38)
                                                                                         gaagccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGa            >  1:2247499/9‑101 (MQ=38)
                                                                                           agccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATc          >  2:49467/7‑101 (MQ=38)
                                                                                             ccagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCCAAAGATGCTGCGATACt        >  2:684492/5‑101 (MQ=37)
                                                                                              tagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       <  2:189596/98‑1 (MQ=38)
                                                                                              cagaGGGGGGCACCGC.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGGCGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       <  1:3212306/98‑1 (MQ=255)
                                                                                              cagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  2:616231/4‑101 (MQ=38)
                                                                                              cagaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTc       >  1:4489018/4‑101 (MQ=38)
                                                                                               agaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGCCGCGCGGGGTGGCGGGCATTACCAGAGATTGCACCAGATGCTGCGAGCCTCg      >  2:1834538/3‑101 (MQ=19)
                                                                                               agaGGGGGGC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTca      >  1:189620/3‑100 (MQ=38)
                                                                                                   gggggC.AGCGCCCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCa  >  1:2038030/1‑101 (MQ=255)
                                                                                                                                                                                                          
AAGCGGACTCCCCCCGGGATATTTGCAGCAAGATGAAACCGGATGCCTTTCATCTTGCCCGAAATATCCCGGGGGGTGAA.TGGC..G.AG.CAGA.AGGGGGGCAAGCG.CCC.CCTGCCCCGGCGGTCCGTGGCGGCGCCTTGACGGGCGGGGGCGCGGGCATTAACCGAAATTGCAACAGATGCTGCGATCCTCGCCCA  >  NC_014034/2493977‑2494170

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG