Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,747,849	+G	100%	intergenic (‑181/+112)	RCAP_rcc02553 ← / ← RCAP_rcc02554	hypothetical protein/NmrA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,747,849	1	.→G	100.0%	59.7	21	intergenic (‑181/+112)	RCAP_rcc02553/RCAP_rcc02554	hypothetical protein/NmrA family protein
Reads supporting (aligned to +/- strand):  new base (2/18):  ref base (0/1):  total (2/19)

GTTCGGGCGGAATAGGAAA.CGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943                                                                                                         |                                                                                                gTTCGGGCGAAATAGGAAA.CGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c                                                                                                  <  1:2132594/101‑1 (MQ=255)   tggggCGAAATAGGAAC.CGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.gg                                                                                               <  2:573818/99‑2 (MQ=255)    cGGGCGGAATAGGAAC.CGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggc                                                                                              <  1:3799118/101‑3 (MQ=255)     gggCGGAATGGGAAC.CGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcg                                                                                             <  2:4063176/101‑4 (MQ=38)      gggggAAAAAGAAACCGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcg                                                                                             <  1:2466891/98‑4 (MQ=37)       ggggAATAGGAAA.CGCGCCCCGCTTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcggg                                                                                           <  1:521111/99‑6 (MQ=38)        gggAATGGGAAA.CGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcgggc                                                                                          <  1:4546021/100‑7 (MQ=37)        gggAATAGGAAA.CCCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCgcccggcc.c.ggcgggc                                                                                          <  1:1108110/100‑7 (MQ=38)           aaggggAAC.CGCCCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgag                                                                                        <  1:3807802/97‑1 (MQ=25)            atgggAAA.CGCGCCCCTCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCgaga                                                                                       <  1:1742254/98‑1 (MQ=25)             aaGGAAA.CGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGaa                                                                                      <  1:1195940/100‑1 (MQ=37)              agaaaa.CGCGCCCCGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAg                                                                                     <  1:3342036/98‑1 (MQ=25)                   a.CGCGCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                <  2:3319303/101‑1 (MQ=25)                   a.CGCCACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                <  2:4642367/101‑1 (MQ=25)                    gcgcgCCCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                <  1:4355321/100‑1 (MQ=25)                    ccccgccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccc                                                                                <  2:2793087/95‑1 (MQ=25)                     cgcgACCCGCGTTT.CCCCGACCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccg                                                                               <  1:1432911/101‑1 (MQ=25)                      gcgCCCCGCTTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgccagg                                                                              <  2:3160504/101‑4 (MQ=21)                       gcgcccCCGTTTCCCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgc                                                                              <  2:4071989/98‑1 (MQ=21)                       ccccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGcgcccgcc                                                                             <  2:3400290/98‑1 (MQ=25)                         ccccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gg                                                                          <  1:2099466/100‑1 (MQ=25)                           ccGCGTTC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggc                                                                        <  2:19395/101‑3 (MQ=25)                            cgcgTGC.CCCCCCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  1:3348533/101‑4 (MQ=21)                            cgcgTCC.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  1:2743619/101‑4 (MQ=21)                             ccttctcccccGCCCGCAGCGCCCGGTTGCGGCATGCCCGCTTCAGCCCGCACCACAGAGGGCGGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggcc                                                                       <  1:2618177/94‑4 (MQ=21)                               ggcccccccGCCCGCAGCGCCCGGTTGCGGCATGGCCCCTTCAGCCCGCACCACAGAGGGCAGCCCCCGGCC.CGGCGGGCGAGAAGCGCCCgcc.gggccgg                                                                     <  1:3540001/96‑6 (MQ=21)                                        gcccgcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGCAGCGCCCGCCGGGCCGGGCGCTGccc                                                             >  1:2730801/1‑101 (MQ=21)                                            gcAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.CGGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGCgg                                                         >  2:17465/1‑101 (MQ=21)                                            cccgcgcCCGGTGCCGGCATGGCGGCTTCAGCCCGCACCACAGAGGGCAGCGCTGGGCCGG.GCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAgg                                                         <  2:201514/98‑1 (MQ=12)                                                                                                  cggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGCCACAGGACCTCTGTGACGCATGGcagaacggaag   >  1:748448/1‑91 (MQ=17)                                                                                                   ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCCTGGTCAAGGCGAtcg  >  2:1929585/1‑99 (MQ=18)                                                                                                   ggcc.cgGCGGGCGAGAAGCGCCCGCCGGGCCGGGCGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATgg  >  1:2973239/1‑101 (MQ=18)                                                                                                         |                                                                                                GTTCGGGCGGAATAGGAAA.CGCGACCCGCGTTT.CCCCGCCCGCAGCGCCCGGTTGCGGCATGACCGCTTCAGCCCGCACCACAGAGGGCAGCGCCCGGCC.C.GCGGCCGAGAAGCGCCCGCC.GGCCGGCTGCTGCCCGAGGGCCTTTGGGCCCTCGGGGGTCACAGGACCTCTGTGACGCATGGCCAAGGCGATGG  >  NC_014034/2747749‑2747943 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG