Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,189 +C 90.4% coding (1332/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1891.→C90.4% 38.3 ‑1.8 0.0146coding (1332/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (68/64):  ref base (4/10):  total (72/74)
Fisher's exact test strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA  >  NC_014034/3256093‑3256277
                                                                                                      |                                                                                                  
ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATgg                                                                                                     >  2:3674309/1‑101 (MQ=255)
ggTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATgg                                                                                                     >  2:1381533/1‑101 (MQ=255)
 gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggc                                                                                                    <  2:1811526/101‑2 (MQ=255)
 gTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggc                                                                                                    <  2:3691924/101‑2 (MQ=255)
   gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcac                                                                                                  >  1:1456001/1‑98 (MQ=255)
   gCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcac                                                                                                  <  1:1793719/101‑4 (MQ=255)
    cAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacg                                                                                                 >  1:2305727/1‑97 (MQ=255)
      gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggc                                                                                               <  2:3341112/101‑7 (MQ=255)
      gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggc                                                                                               >  1:1909274/1‑95 (MQ=255)
      gcgcGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggc                                                                                               <  2:4303951/101‑7 (MQ=255)
       cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggct                                                                                              <  1:444872/101‑8 (MQ=255)
       cgcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggct                                                                                              >  2:1579596/1‑94 (MQ=255)
        gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctg                                                                                             <  2:2509045/101‑9 (MQ=255)
        gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctg                                                                                             <  1:2880848/101‑9 (MQ=255)
        gcgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGAACCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctg                                                                                             >  2:2171284/1‑93 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                            <  1:2700696/101‑10 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                            >  1:1754873/1‑92 (MQ=255)
         cgTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctgg                                                                                            <  1:376228/101‑10 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctggc                                                                                           >  1:3179744/1‑91 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctggc                                                                                           >  2:3558680/1‑91 (MQ=255)
          gTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATggcacggctggc                                                                                           >  2:2176497/1‑91 (MQ=255)
                 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                                >  2:792740/1‑99 (MQ=255)
                 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                                >  2:1977178/1‑99 (MQ=255)
                   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                                              >  2:838573/1‑97 (MQ=255)
                   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                                              >  1:4513077/1‑97 (MQ=255)
                    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.g.ttgctc                                                                             <  2:687556/101‑6 (MQ=255)
                     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcg                                                                            <  2:4535661/101‑7 (MQ=255)
                      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                           >  1:990426/1‑94 (MQ=255)
                        gCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGccc                                                                          <  1:61463/101‑1 (MQ=255)
                         cTTTTGCTCAAGGGCGGCGGGCACCGCGTGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                                         <  2:3305212/101‑1 (MQ=255)
                         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                                         <  2:1606311/101‑1 (MQ=255)
                         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCg                                                                         <  2:2618084/101‑1 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCgg                                                                        >  1:829929/1‑101 (MQ=255)
                          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCgg                                                                        >  2:871537/1‑101 (MQ=255)
                             tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..g                                                                   <  2:4384814/101‑1 (MQ=255)
                               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                                                 >  2:2062915/1‑101 (MQ=255)
                               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                                                 <  2:1286207/101‑1 (MQ=255)
                                 cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCCGCA..GGgcg                                                               >  1:3013521/1‑101 (MQ=255)
                                  caGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GATGCGCGCCCCGCA..GGgcgg                                                              >  2:1467636/2‑100 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..gggggg                                                              >  1:689307/1‑98 (MQ=255)
                                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGgcgg                                                              >  2:2787796/1‑100 (MQ=255)
                                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGGG.G.TGCTCGCCCGGCA..GGGCGC.gg                                                           >  2:4084916/1‑101 (MQ=255)
                                    ggacggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGC.gg                                                           >  1:2463054/4‑101 (MQ=255)
                                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..ggggggcggga                                                         >  1:3241911/1‑93 (MQ=255)
                                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggca                                                        <  2:2628452/101‑4 (MQ=255)
                                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCCGCC..GGGGGC.ggggc                                                        >  2:4067713/1‑98 (MQ=255)
                                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcga                                                       >  2:519006/1‑97 (MQ=255)
                                         gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                                      <  2:3549911/101‑6 (MQ=255)
                                          cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCC..GGGCGC.ggggaagg                                                     >  2:3095308/1‑95 (MQ=255)
                                          cggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gg                                                    <  2:4156024/101‑1 (MQ=255)
                                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.ggg                                                   >  2:2018647/1‑100 (MQ=255)
                                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.ggg                                                   <  1:3795079/101‑2 (MQ=255)
                                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGAC.GAGG.TGCTCGCCCGGGA..GGGCGC.gggggacggc                                                   >  2:3057970/1‑93 (MQ=255)
                                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               >  1:286604/1‑96 (MQ=255)
                                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               <  2:2724240/101‑6 (MQ=255)
                                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCNCGGGCA.c.gggcgggccg                                            >  2:1548092/1‑93 (MQ=255)
                                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                                             <  2:432383/101‑1 (MQ=255)
                                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                                             >  1:4273433/1‑101 (MQ=255)
                                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggggggcccgccg                                        >  2:3448740/1‑92 (MQ=255)
                                                       gCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGa                                        <  1:1545486/101‑1 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGCGG.GGCC.G.GGCGGCCCGGCCGAt                                       >  2:3621401/1‑101 (MQ=255)
                                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGGCCGGCCggt                                       >  2:144133/1‑99 (MQ=255)
                                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCGGATc                                      <  2:1266917/101‑1 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATcc                                     >  2:1163564/1‑100 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  2:2093294/101‑1 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:1977313/101‑1 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:728597/101‑1 (MQ=255)
                                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:3626761/101‑1 (MQ=255)
                                                           cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGGACC.GGCGGCCCCGCCGGTCTg                                    >  2:3822876/1‑101 (MQ=255)
                                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                  >  1:2203754/1‑101 (MQ=255)
                                                               tCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGGGGCCCGGCCGGGCTGCggg                                >  1:62098/1‑100 (MQ=255)
                                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                               <  1:822589/101‑1 (MQ=255)
                                                                  gACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                             <  1:178329/101‑1 (MQ=255)
                                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCNGCCGCTCTGCGGCTGGa                            >  2:2235693/1‑101 (MQ=255)
                                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGGTCTGCGGCCggg                            >  1:3416844/1‑100 (MQ=255)
                                                                     cctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                          <  1:923382/97‑1 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCC.CGGGGGGGCCGGCCGATCTTCGGCCGGGCgg                         >  2:288264/4‑101 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACgg                         <  2:3179501/98‑1 (MQ=255)
                                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCACGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCCGGGCgg                         >  1:3947938/4‑101 (MQ=255)
                                                                        ggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCCGCCGATCTGCGGCTGGACgggg                       >  2:1531256/2‑100 (MQ=255)
                                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                      <  1:1481791/101‑1 (MQ=255)
                                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                      <  1:2082738/101‑1 (MQ=255)
                                                                            gaggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCt                   <  1:257630/91‑1 (MQ=255)
                                                                             aggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                  <  1:1767024/92‑1 (MQ=255)
                                                                               gcgcggctGGAA.CCGGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                <  2:1046333/94‑1 (MQ=255)
                                                                               gcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                <  1:2149225/94‑1 (MQ=255)
                                                                                  cagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGTCCGATCTGCGGCTGGACGGGCTCCTGATGcc             <  1:4141513/97‑1 (MQ=255)
                                                                                     ctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg          <  1:1070463/100‑1 (MQ=255)
                                                                                      tGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGGTGCCcggg         >  1:4235307/1‑100 (MQ=255)
                                                                                      tGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgc         <  1:2787994/101‑1 (MQ=255)
                                                                                       ggaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg        >  2:2153118/6‑101 (MQ=255)
                                                                                        gaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGGTGCCGGgcgc       >  1:3497802/5‑101 (MQ=255)
                                                                                        gaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGCTCCTGATGCCGGgcgc       >  2:4408396/5‑101 (MQ=255)
                                                                                         aaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCTCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      <  1:4003155/98‑1 (MQ=255)
                                                                                         aaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      <  1:1130446/98‑1 (MQ=255)
                                                                                         aaccc.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      >  1:3127101/4‑101 (MQ=255)
                                                                                          accc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg     <  1:3078260/99‑1 (MQ=255)
                                                                                          accc.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGCCCCGGTGCCCGGcgccc     >  1:3195257/3‑100 (MQ=255)
                                                                                           ccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc    <  1:1355033/100‑1 (MQ=255)
                                                                                             c.gcgATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCGGGggccgggg  >  1:3322884/1‑98 (MQ=255)
                                                                                                      |                                                                                                  
GGTGCAGCGCGTCGCGGCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA  >  NC_014034/3256093‑3256277

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG