Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,256,195 +G 100% coding (1338/1734 nt) recJ → single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,256,1951.→G100.0% 535.7 142coding (1338/1734 nt)recJsingle‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (68/74):  ref base (0/0):  total (68/74)

Only 100 of 148 total aligned reads displayed.

GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA  >  NC_014034/3256109‑3256277
                                                                                              |                                                                                          
gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.tt                                                                                 >  1:713032/1‑100 (MQ=255)
gCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.g.tt                                                                                 <  1:4376935/101‑2 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                                >  2:792740/1‑99 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                                >  2:1977178/1‑99 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttg                                                                                >  2:1027568/1‑99 (MQ=255)
 ccGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.g.ttg                                                                                <  2:4397529/101‑3 (MQ=255)
  cGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgc                                                                               >  1:378386/1‑98 (MQ=255)
   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                                              >  1:3086159/1‑97 (MQ=255)
   gAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGCCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgct                                                                              >  1:4513077/1‑97 (MQ=255)
    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctc                                                                             >  2:4315415/1‑96 (MQ=255)
    aGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.g.ttgctc                                                                             <  2:687556/101‑6 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcg                                                                            >  1:1838372/1‑95 (MQ=255)
     ggggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcg                                                                            <  2:4535661/101‑7 (MQ=255)
      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                           >  2:1397078/1‑94 (MQ=255)
      gggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgc                                                                           >  2:4002870/1‑94 (MQ=255)
       ggCTTTTGGTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgcc                                                                          <  2:4487207/101‑9 (MQ=255)
       ggCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.g.ttgctcgcc                                                                          >  2:2086287/1‑93 (MQ=255)
         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GAGG.TGCTCGcccc                                                                         >  2:2907383/1‑100 (MQ=255)
         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCg                                                                         <  2:1606311/101‑1 (MQ=255)
         cTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCg                                                                         <  2:2618084/101‑1 (MQ=255)
          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCgg                                                                        >  1:829929/1‑101 (MQ=255)
          ttttGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCgg                                                                        >  2:871537/1‑101 (MQ=255)
             tGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..g                                                                   <  2:4384814/101‑1 (MQ=255)
              gCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..gg                                                                  <  2:83276/101‑1 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                                                 >  2:2062915/1‑101 (MQ=255)
               cTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..ggg                                                                 <  2:1286207/101‑1 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCC..gggg                                                                >  2:2512960/1‑100 (MQ=255)
                tCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCCCCCGGCA..GGgc                                                                >  1:2418843/1‑101 (MQ=255)
                 cAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCCGCA..GGgcg                                                               >  1:3013521/1‑101 (MQ=255)
                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGgcgg                                                              >  2:2787796/1‑100 (MQ=255)
                  aaGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGgcgc                                                              >  1:2671207/1‑101 (MQ=255)
                   aGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGcgc.g                                                            <  2:163518/101‑1 (MQ=255)
                    gggCGGCGGGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCCGGG.G.TGCTCGCCCGGCA..GGGCGC.gg                                                           >  2:4084916/1‑101 (MQ=255)
                    ggacggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGC.gg                                                           >  1:2463054/4‑101 (MQ=255)
                       cggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggca                                                        <  2:272399/101‑4 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGC.ggggac                                                       >  1:4143415/1‑97 (MQ=255)
                        ggcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcga                                                       >  2:519006/1‑97 (MQ=255)
                         gcggGCACCGCATGGCCGCGGGTCTGCCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                                      <  1:1472113/101‑6 (MQ=255)
                         gcggGCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.gggcacg                                                      <  2:3549911/101‑6 (MQ=255)
                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.ggg                                                   <  1:3795079/101‑2 (MQ=255)
                           gggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGC.ggggggggg                                                    >  1:3320058/1‑94 (MQ=255)
                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggc                                                  >  1:3413846/1‑99 (MQ=255)
                            ggCACCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGAC.GAGG.TGCTCGCCCGGGA..GGGCGC.gggggacggc                                                   >  2:3057970/1‑93 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               <  2:1978713/101‑6 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               <  2:2724240/101‑6 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               >  1:286604/1‑96 (MQ=255)
                               aCCGCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggcggc                                               <  2:3557927/101‑6 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                                             <  1:2703498/101‑1 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                                             >  1:4273433/1‑101 (MQ=255)
                                  gCATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccg                                             >  2:1504866/1‑101 (MQ=255)
                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccgg                                            <  1:505880/101‑1 (MQ=255)
                                   cATGGCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGcggcccgg                                            <  2:1277652/101‑1 (MQ=255)
                                      ggCCGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.c.gggggggcccgccg                                        >  2:3448740/1‑92 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCAGGGGGCGG.GGCC.G.GGCGGCCCGGCCGAt                                       >  2:3621401/1‑101 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGCGGGCA.CGGGGGGGCCGGCCGAt                                       >  1:2839348/1‑101 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGGCCGGCCggt                                       >  2:144133/1‑99 (MQ=255)
                                        ccGCGGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGAt                                       <  1:681689/101‑1 (MQ=255)
                                         cgcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCGGATc                                      <  2:1266917/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:3626761/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  2:368788/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:2176647/101‑1 (MQ=255)
                                          gcgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCt                                     <  1:728597/101‑1 (MQ=255)
                                           cgGGTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGGACC.GGCGGCCCCGCCGGTCTg                                    >  2:3822876/1‑101 (MQ=255)
                                            gggTCTGCCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGc                                   <  2:3135420/101‑1 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                  <  1:3024207/101‑1 (MQ=255)
                                             ggTCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCg                                  >  1:2203754/1‑101 (MQ=255)
                                               tCTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGGGGCCCGGCCGGGCTGCggg                                >  1:62098/1‑100 (MQ=255)
                                                cTGACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCt                               <  1:822589/101‑1 (MQ=255)
                                                 tgcccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTg                              <  2:859482/93‑1 (MQ=255)
                                                  gACCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTgg                             <  1:178329/101‑1 (MQ=255)
                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCNGCCGCTCTGCGGCTGGa                            >  2:2235693/1‑101 (MQ=255)
                                                   aCCCTGGCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTggg                            >  2:2149075/1‑100 (MQ=255)
                                                    ccctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGAc                           <  2:3319809/96‑1 (MQ=255)
                                                     cctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACg                          <  1:923382/97‑1 (MQ=255)
                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGGA.CGGGCGGCCCGGCCGGTCTGCGGCCgggccg                         >  1:931381/4‑97 (MQ=255)
                                                      ctggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACgg                         <  2:3179501/98‑1 (MQ=255)
                                                       tggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACggg                        <  2:4143127/99‑1 (MQ=255)
                                                        ggCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCCGCCGATCTGCGGCTGGACgggg                       >  2:1531256/2‑100 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                      <  1:1481791/101‑1 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                      <  1:3213608/101‑1 (MQ=255)
                                                         gCCGAGGCGCAGCTGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCt                      <  1:2082738/101‑1 (MQ=255)
                                                             aggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTg                  <  1:1767024/92‑1 (MQ=255)
                                                              ggcgcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGa                 <  1:400094/93‑1 (MQ=255)
                                                               gcgcggctGGAA.CCGGCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGAt                <  2:1046333/94‑1 (MQ=255)
                                                                 gcagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGCTCTGCGGCTGGACGGGCTCCTGATGc              >  2:2817874/6‑101 (MQ=255)
                                                                  cagctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGTCCGATCTGCGGCTGGACGGGCTCCTGATGcc             <  1:4141513/97‑1 (MQ=255)
                                                                     ctGGAACCC.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCggg          <  1:1070463/100‑1 (MQ=255)
                                                                       ggaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGGGGGCTCCTGATGCCggggc        >  2:4188953/6‑99 (MQ=255)
                                                                       ggaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGgcg        >  2:2153118/6‑101 (MQ=255)
                                                                        gaaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGGCCTGGTGCCGGgcgc       >  1:3497802/5‑101 (MQ=255)
                                                                         aaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCTCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      <  1:4003155/98‑1 (MQ=255)
                                                                         aaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      <  1:1130446/98‑1 (MQ=255)
                                                                         aaccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      <  2:3265620/98‑1 (MQ=255)
                                                                         aaccc.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgcc      >  1:3127101/4‑101 (MQ=255)
                                                                          accc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg     <  2:2203604/99‑1 (MQ=255)
                                                                          accc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccg     <  1:2065422/99‑1 (MQ=255)
                                                                          accc.GCGATGG.CACGGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGGGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGCCCCGGTGCCCGGcgccc     >  1:3195257/3‑100 (MQ=255)
                                                                           ccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc    <  1:1579700/100‑1 (MQ=255)
                                                                           ccc.GCGATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGcgccgc    <  1:1355033/100‑1 (MQ=255)
                                                                             c.gcgATGGCAC.GGCTGGCC.GA.GTTGCTCGCCCGGCA..GGGCGCGGGCA.CGGGCGGCCCGGCCGATCTGCGGCTGGGCGGGGTCCCGGTGCCGGGggccgggg  >  1:3322884/1‑98 (MQ=255)
                                                                                              |                                                                                          
GCCGAGGGGCTTTTGCTCAAGGGCGGCGGGCACCGCATGGCCGCGGGTCTGACCCT.GCCGAGG.GCAG.TGGAA.CC.GCGATGG.AA.GGCT.GCC.GA.G.TGTTCGCCCGGCA..GGGCGC.GGCA.C.GGCGGCCCGGCCGATCTGCGGCTGGACGGGCTCCTGATGCCGGGCGCCGCGA  >  NC_014034/3256109‑3256277

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG