Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,573 +C 9.8% coding (1034/6438 nt) RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5731.→C 9.8% 71.2 ‑3.5 ‑0.4246coding (1034/6438 nt)RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (5/19):  ref base (98/124):  total (103/143)
Fisher's exact test strand distribution p-value = 3.02e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.67e-01

Only 100 of 257 total aligned reads displayed.

CTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGA  >  NC_014034/682473‑682673
                                                                                                     |                                                                                                    
 tcctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.t                                                                                                     >  2:2859175/1‑101 (MQ=255)
 tcctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.t                                                                                                     >  1:3858103/1‑101 (MQ=255)
  cctcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.Tg                                                                                                    <  2:4322675/101‑1 (MQ=255)
    tcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCt                                                                                                  >  2:1399925/1‑101 (MQ=255)
    tcGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCt                                                                                                  >  2:2675656/1‑101 (MQ=255)
    ccGGACAGCGCCGCGACCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCCTCTGCCCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCt                                                                                                  <  1:1395674/100‑1 (MQ=255)
     cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                                                                                 <  1:3402281/101‑1 (MQ=255)
     cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                                                                                 >  1:2886689/1‑101 (MQ=255)
      ggACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCa                                                                                                >  1:3720385/1‑101 (MQ=255)
        aCAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGc                                                                                              >  2:289818/1‑101 (MQ=255)
          aGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGccc                                                                                            <  2:2874031/101‑1 (MQ=255)
            cgccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCgg                                                                                          >  1:944043/1‑101 (MQ=255)
             gccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                                                                         >  2:2431900/1‑101 (MQ=255)
              ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                                                                        <  2:1245628/101‑1 (MQ=255)
                 cgATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATg                                                                                     <  2:4447415/101‑1 (MQ=255)
                  gATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGt                                                                                    >  1:2032479/1‑101 (MQ=255)
                    tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                                                                                  <  2:2534291/101‑1 (MQ=255)
                    tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                                                                                  >  1:3106339/1‑101 (MQ=255)
                      tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                                                >  1:4225942/1‑101 (MQ=255)
                      tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                                                >  2:2569240/1‑101 (MQ=255)
                      tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                                                <  2:71554/101‑1 (MQ=255)
                       gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                                               >  1:1787036/1‑101 (MQ=255)
                        ccGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCgg                                                                              >  1:1222253/1‑101 (MQ=255)
                         cgcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                                                                             <  1:1086165/101‑1 (MQ=255)
                          gcgTGCCCAGACCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTc                                                                            <  1:3217071/101‑1 (MQ=255)
                           cgTGCCCAGACCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                                                           <  2:1837534/101‑1 (MQ=255)
                           cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                                                           <  1:748541/101‑1 (MQ=255)
                            gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                                                                          >  2:219567/1‑101 (MQ=255)
                              gCCCAGAACCCCCAGCAGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                                                                        >  1:4073731/1‑101 (MQ=255)
                                  aGAGCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                                                                    <  1:1493073/101‑1 (MQ=255)
                                  aGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                                                                    >  2:652004/1‑101 (MQ=255)
                                   gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc                                                                   <  2:23373/101‑1 (MQ=255)
                                    cgccgccAGCTGGCCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                                                  <  1:3419186/96‑1 (MQ=25)
                                    cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                                                  <  1:1003370/96‑1 (MQ=37)
                                    aaCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCt                                                                  >  1:1750400/1‑101 (MQ=255)
                                     gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                                 <  2:1343206/97‑1 (MQ=37)
                                     gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                                 <  2:3735809/97‑1 (MQ=37)
                                     gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                                 <  2:3274461/97‑1 (MQ=37)
                                     gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                                 >  1:135916/5‑101 (MQ=37)
                                      ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg                                                                <  1:1718649/98‑2 (MQ=37)
                                      cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                                                <  1:45412/101‑1 (MQ=255)
                                      cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc                                                                >  2:2408093/1‑101 (MQ=255)
                                       ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                                               <  2:1114567/101‑1 (MQ=255)
                                        gccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAg                                                              >  1:2592368/2‑101 (MQ=37)
                                         ccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgc                                                             <  1:2909646/101‑1 (MQ=25)
                                          cAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcg                                                            >  2:3834459/1‑101 (MQ=255)
                                           aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc                                                           <  2:85535/101‑1 (MQ=255)
                                           aGCTGGGCCGGGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc                                                           <  2:2170620/101‑1 (MQ=255)
                                            gCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGcgcg                                                          >  1:1807567/1‑101 (MQ=255)
                                             cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg                                                         <  1:2275677/101‑1 (MQ=255)
                                              tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                        >  1:4177620/1‑101 (MQ=255)
                                              tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                        >  1:3531961/1‑101 (MQ=255)
                                              tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                        <  1:266575/101‑1 (MQ=255)
                                              tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                        >  1:533009/1‑101 (MQ=255)
                                               gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg                                                       <  1:4052861/101‑1 (MQ=255)
                                               gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg                                                       >  2:4202297/1‑101 (MQ=255)
                                                 gCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGcggcggg                                                     >  2:1369572/1‑100 (MQ=255)
                                                 gCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgccact                                                     <  2:4524971/101‑6 (MQ=21)
                                                   cGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGc                                                   <  2:775294/101‑1 (MQ=255)
                                                    gTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGcc                                                  >  2:1638040/1‑101 (MQ=255)
                                                     tGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCg                                                 >  1:2099462/1‑101 (MQ=255)
                                                      gCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGa                                                >  2:3675891/1‑101 (MQ=255)
                                                       cTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGAt                                               <  1:619224/101‑1 (MQ=255)
                                                        tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                                              >  2:209848/1‑101 (MQ=255)
                                                        tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                                              <  1:1453706/101‑1 (MQ=255)
                                                          ccgatctccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgc                                            <  1:3954414/94‑1 (MQ=255)
                                                            cgccgcCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCCGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccc                                          >  2:611837/1‑100 (MQ=255)
                                                             gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc                                         <  1:1605061/101‑1 (MQ=255)
                                                             gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc                                         >  2:4627122/1‑101 (MQ=255)
                                                             gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc                                         <  2:306475/101‑1 (MQ=255)
                                                              ccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGGGCCGATCGCCGCa                                        >  1:4311921/1‑101 (MQ=255)
                                                                gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                                      <  1:2812660/101‑1 (MQ=255)
                                                                gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                                      >  2:690988/1‑101 (MQ=255)
                                                                  cATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAgg                                    >  2:4325701/1‑101 (MQ=255)
                                                                   aTCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGc                                   <  2:943963/101‑1 (MQ=255)
                                                                   aTCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGc                                   >  1:874563/1‑101 (MQ=255)
                                                                   aTCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGc                                   >  2:2275400/1‑101 (MQ=255)
                                                                    tCTGCGCCCCGGTCAGCGCCTCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGcc                                  <  2:1489092/101‑1 (MQ=255)
                                                                    tCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGcc                                  >  1:2716925/1‑101 (MQ=255)
                                                                    tCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGcc                                  <  2:2949676/101‑1 (MQ=255)
                                                                     cTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcg                                 <  1:2941283/101‑1 (MQ=255)
                                                                      tGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcgc                                >  2:2648351/1‑101 (MQ=255)
                                                                             cGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaa                         <  1:233967/101‑1 (MQ=255)
                                                                              ggTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaa                        <  1:1288526/101‑1 (MQ=255)
                                                                               gTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaaa                       <  2:3751192/101‑1 (MQ=255)
                                                                                   gcgcCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGgcg                   <  2:1807441/101‑1 (MQ=255)
                                                                                      ccgccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCCGAAAAGGCGCCg                <  1:2530244/101‑1 (MQ=255)
                                                                                       cgccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGa               <  2:394104/101‑1 (MQ=255)
                                                                                        gccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGAt              <  1:3913906/101‑1 (MQ=255)
                                                                                         ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc             <  1:4627423/101‑1 (MQ=255)
                                                                                         ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc             >  2:1198627/1‑101 (MQ=255)
                                                                                         ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc             <  2:2032339/101‑1 (MQ=255)
                                                                                           aGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTg           <  1:681495/101‑1 (MQ=255)
                                                                                              tCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTc        <  2:3106095/101‑1 (MQ=255)
                                                                                              tCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTc        >  2:3489768/1‑101 (MQ=255)
                                                                                               cGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCg       >  2:4115427/1‑101 (MQ=255)
                                                                                               cGATCG.TGCTCAACCCGGCCGTGTCCTCGGGCGAAAGCCCCTTGAGCGGGGTGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCg       <  2:3861608/101‑1 (MQ=255)
                                                                                                gATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGTTCgg      >  2:1701635/1‑101 (MQ=255)
                                                                                                gATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCgg      <  2:2792625/101‑1 (MQ=255)
                                                                                                  tCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGcc    <  2:1458223/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
CTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGA  >  NC_014034/682473‑682673

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG