New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	= 1224266	205 (0.960)	40 (0.190)	11/176	27.6	0.18	intergenic (‑138/+31)	hisF/hisA	imidazole glycerol phosphate synthase subunit HisF/1‑(5‑phosphoribosyl)‑5‑[(5‑ phosphoribosylamino)methylideneamino] imidazole‑4‑carboxamide isomerase
	?	NC_014034	3011299 =	172 (0.720)					intergenic (+98/+44)	purK/RCAP_rcc02822	phosphoribosylaminoimidazole carboxylase ATPase subunit/lipase
Rejected: Position hash score below cutoff.

GGCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224157‑1224266 ....................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398                                                                                                                                                                                                                              GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  2:4386538/101‑1 (MQ=1)           GGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     <  1:2275392/101‑1 (MQ=1)           GGCTTGGAAAGAGTCGAGCGGGGCTCTGCCCCGCAACCCGGGATATTTGTGGAAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGGA                                                                                                     >  2:3104258/1‑101 (MQ=0)                                                                                 ctTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGC                               >  2:4484693/3‑101 (MQ=255)                                                                                 ctTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGC                               >  2:3518971/3‑101 (MQ=255)                                                                                 ctTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGC                               >  1:3582480/3‑101 (MQ=255)                                                                                  tTTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCC                              >  1:3943594/2‑101 (MQ=255)                                                                                   TTCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCG                             >  1:1465190/1‑101 (MQ=255)                                                                                    tcacctggggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            <  2:2917231/91‑1 (MQ=255)                                                                                    TCACCTTGGGACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGC                            >  1:2479717/1‑101 (MQ=255)                                                                                      accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          >  2:4056526/9‑101 (MQ=255)                                                                                      accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          >  1:2995624/9‑101 (MQ=255)                                                                                      accttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGC                          >  1:393626/9‑101 (MQ=255)                                                                                        cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        >  2:3758132/7‑101 (MQ=255)                                                                                        cttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCC                        <  2:2719876/95‑1 (MQ=255)                                                                                         ttgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       >  2:132207/6‑101 (MQ=255)                                                                                         tggggACAAATACCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCC                       <  1:4308578/96‑1 (MQ=255)                                                                                          tgggACAGATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCc                      >  2:964110/5‑100 (MQ=255)                                                                                          tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      >  1:1178528/5‑101 (MQ=255)                                                                                          tgggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCA                      >  1:2417208/5‑101 (MQ=255)                                                                                           gggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAG                     >  2:1297229/4‑101 (MQ=38)                                                                                            ggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGC                    >  1:2657154/3‑101 (MQ=38)                                                                                            ggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGC                    >  1:249401/3‑101 (MQ=38)                                                                                            ggACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAAGCCGCGCCCCAGC                    >  2:960130/3‑101 (MQ=255)                                                                                              ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCCGCCC                  >  2:1345586/1‑101 (MQ=38)                                                                                              ACAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCC                  >  2:469285/1‑101 (MQ=38)                                                                                               CAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCG                 >  2:857972/1‑101 (MQ=37)                                                                                               CAAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCG                 >  2:1459349/1‑101 (MQ=37)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGGCGCTGCAAGGCCGCGCCCCAGCCCGG                >  2:4634862/1‑101 (MQ=25)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTTCAAAGCCGCGCCCCAGCCCcg                >  1:888884/1‑99 (MQ=255)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGGCGCGCCCCAGCCCGG                >  2:367461/1‑101 (MQ=25)                                                                                                AAATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGG                >  2:439183/1‑101 (MQ=35)                                                                                                 AATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGC               >  2:4055953/1‑101 (MQ=35)                                                                                                 AATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGC               >  2:67948/1‑101 (MQ=35)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  1:3068588/1‑101 (MQ=35)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  2:1887576/1‑101 (MQ=35)                                                                                                  ATATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCA              >  2:133847/1‑101 (MQ=35)                                                                                                   TATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCAC             >  1:1802575/1‑101 (MQ=35)                                                                                                    ATCCGGGGGGGAGACTTGCCGCAGGAAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            <  1:223224/101‑1 (MQ=255)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCCGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            >  1:1598563/1‑101 (MQ=25)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            >  2:3132314/1‑101 (MQ=35)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            >  1:2026388/1‑101 (MQ=35)                                                                                                    ATCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACC            <  1:4523846/101‑1 (MQ=35)                                                                                                                                                                                                                    GGCGTCTTGCGGCTTGGAAAGCGTCGAGCGGGGCTCTGCCCCGCACCCCGGGATATTTGTGGCAAGATGAAACCGAAGCCTCTTCATCTTGCCACAAATATCCCGGGGGG....................................................................................................  >  NC_014034/1224157‑1224266 ....................................................................................................TCCCGGGGGGAGACTTGCCGCAGGCAAGAGGGGGGCAGCGCCCCCCTGCCCGGCTCACGGCAGGGCCAGCCGCTGCAAGGCCGCGCCCCAGCCCGGCACCGGCTCGAACT  >  NC_014034/3011299‑3011398 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG