New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	390805 =	45 (0.250)	37 (0.210)	22/150	17.6	0.42	intergenic (‑121/+168)	mipZ/rpmE	chromosome partitioning protein MipZ/50S ribosomal protein L31
	?	NC_014034	1732895 =	59 (0.250)					intergenic (‑319/‑84)	RCAP_rcc01601/tgt	FeS assembly SUF system protein/queuine tRNA‑ribosyltransferase
Rejected: Position hash score below cutoff.

CCCTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCAGCGTATTTTGCCAAGGAAAAGGGGGGCGGAGCCC  >  NC_014034/390705‑390897                                                                                                     |                                                                                             cccTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGgtgt                                                                                              <  2:296591/101‑1 (MQ=255) cccTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGgtgt                                                                                              <  2:4605206/101‑1 (MQ=255) cccTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGgtgt                                                                                              <  2:3742099/101‑1 (MQ=255)   cTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGttt                                                                                            <  2:3221595/101‑1 (MQ=255)   cTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGttt                                                                                            <  2:1755946/101‑1 (MQ=255)    tCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTc                                                                                           <  1:1103464/101‑1 (MQ=255)    tCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTc                                                                                           <  1:3912357/101‑1 (MQ=255)     cAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCg                                                                                          <  1:1324325/101‑1 (MQ=255)     cAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCg                                                                                          <  1:1188104/101‑1 (MQ=255)     cAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCg                                                                                          <  2:1964665/101‑1 (MQ=255)     cAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCg                                                                                          >  1:3217639/1‑101 (MQ=255)     cAGACGGCCCGCGCGTTTATAGGACGCTCCGGCGTCCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCg                                                                                          <  1:4004699/101‑1 (MQ=255)        aCGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCt                                                                                       <  2:3768014/101‑1 (MQ=255)         cGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTc                                                                                      <  1:4528058/101‑1 (MQ=255)          ggCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCg                                                                                     <  1:1814668/101‑1 (MQ=255)          ggCCCGCGCG.TTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCgg                                                                                    <  2:4387768/101‑1 (MQ=255)              cgcgcgTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCa                                                                                 <  1:1988714/101‑1 (MQ=255)               gcgcgTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGATCGGTCGCGGGGGTGTTTCGCCTCGGGCaa                                                                                <  1:649685/101‑1 (MQ=255)               gcgcgTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCaa                                                                                <  1:3431195/101‑1 (MQ=255)                cgcgTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAg                                                                               <  2:277579/101‑1 (MQ=255)                     ttATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGa                                                                          <  2:3177544/101‑1 (MQ=255)                     ttATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGa                                                                          >  1:481371/1‑101 (MQ=255)                      tataGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAg                                                                         <  2:2144513/101‑1 (MQ=255)                      tataGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAg                                                                         <  1:2595661/101‑1 (MQ=255)                      tataGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAg                                                                         <  1:1077895/101‑1 (MQ=255)                       ataGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAgg                                                                        <  2:4354122/101‑1 (MQ=255)                        taGGACGCTCGGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  1:1979457/101‑1 (MQ=255)                        taGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  2:3065333/101‑1 (MQ=255)                        taGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  2:3624066/101‑1 (MQ=255)                        taGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  2:4067133/101‑1 (MQ=255)                        taGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  1:150234/101‑1 (MQ=255)                        taGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGc                                                                       <  1:1150394/101‑1 (MQ=255)                           gACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGAc                                                                    <  2:1424834/101‑1 (MQ=255)                             cGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGAccc                                                                  <  1:1367726/101‑1 (MQ=255)                             cGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGAccc                                                                  <  2:1387851/101‑1 (MQ=255)                              tcTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  2:141185/100‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGTGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  2:1093477/101‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  1:730542/101‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  2:3457049/101‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  1:2011940/101‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  1:293034/101‑1 (MQ=255)                              gCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcg                                                                 <  1:411423/101‑1 (MQ=255)                               cTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcgc                                                                <  2:2791920/101‑1 (MQ=255)                               cTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcgc                                                                <  1:1853836/101‑1 (MQ=255)                               cTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcga                                                                >  1:141199/1‑100 (MQ=255)                                tCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcgcg                                                               <  1:1337069/101‑1 (MQ=255)                                gccGGGTCCCGAAGGAAGCGCGCCGGGGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCcgcg                                                               <  1:564176/100‑1 (MQ=255)                                 cgggCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCgg                                                              <  2:2944726/99‑1 (MQ=255)                                  cGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCggg                                                             <  2:2561011/101‑1 (MQ=255)                                   cgatcgcGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGACCGGTCGGGGGGGTGTTTCGCCTGGGGCAAGCCCGAGGCGACCCGCgggg                                                            <  2:3487760/95‑1 (MQ=38)                                    gCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCCCCTCGGGCAAGCCCGAGGCGACCCGCggggg                                                           <  1:4167312/101‑1 (MQ=255)                                     cTACCGAAGGAAGCGCGCGGGTGCAAGGGGGGAGGCGAAAAGGGTTTGAACGGTCGGGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCgggggg                                                          <  2:1644292/101‑1 (MQ=255)                                     cTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCgggggg                                                          <  2:3688695/101‑1 (MQ=255)                                      tACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGc                                                         <  2:646352/101‑1 (MQ=255)                                        ccGAAGGAGGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCtt                                                       <  2:2541793/101‑1 (MQ=255)                                        ccGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCtt                                                       <  2:1710959/101‑1 (MQ=255)                                        ccGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCtt                                                       <  2:515252/101‑1 (MQ=255)                                         cGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCggggagatcg                                                      >  1:3488018/1‑95 (MQ=255)                                         cGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTc                                                      <  1:4319340/101‑1 (MQ=255)                                         cGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTc                                                      <  2:4515918/101‑1 (MQ=255)                                         cGAAGGAAGCGCGCCGGGGCAAGGGGGGGGGCGTAGAGGGTTTGAACGTTCGCGGGGGTTTTTCGCTTGGGGCAAGCCGGAGGCGACCCGCgggggagttc                                                      <  2:148226/101‑6 (MQ=255)                                            aggaagCGCGCGGGTGCAGGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGcc                                                   <  2:1052426/101‑1 (MQ=255)                                             ggaagCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGccc                                                  <  1:4365598/101‑1 (MQ=255)                                              gaagCGCGCCGGTGCAGGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGcccc                                                 <  2:2095635/101‑1 (MQ=255)                                                 gcgcgcGGGTGCAGGGGGGGGGGCGAAAGGGGTTTGAGCGGTCGGGGGGGTGTTTCGCCTGGGGCAAGCCCGGGGCGACCCGCGGGGGGCTTCGCCCCCCg                                              <  2:882259/101‑1 (MQ=21)                                                 gcgcgcGGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCg                                              <  2:3217396/101‑1 (MQ=255)                                                 gcgcgcCGGTGCAGGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCg                                              <  2:3323183/101‑1 (MQ=255)                                                   gggcgggTGCAAGGGGGGAGGCGAAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCGGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCa                                            <  2:4607406/96‑1 (MQ=255)                                                   gcgcCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCa                                            <  2:2356355/101‑1 (MQ=255)                                                    ggcCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGACGGGTCGCGGGGGTGTTGCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCAc                                           <  1:2009990/100‑1 (MQ=255)                                                    cgcCGGGGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCAGCAc                                           <  1:2300173/101‑1 (MQ=255)                                                       cGGGGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCAcccc                                        <  1:667403/101‑1 (MQ=255)                                                        ggTGCAAGGGGGGAGGCGCAAAGGGTTTGACCGGTCGGGGGGGTGTTTCGCCCCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCAccccc                                       <  1:2506715/101‑1 (MQ=255)                                                           gCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCa                                    <  2:1069651/101‑1 (MQ=255)                                                                       gcgcAAAGGGTTTGAACGGTCGGGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCAGCGTATTTTGcc                        <  1:4666997/101‑1 (MQ=255)                                                                              gggTTGGAACGGTGGGGGGGGTGTTTCGCCCCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCAGCGTATTTTGCCAAGGaaa                 <  2:1491203/101‑1 (MQ=18)                                                                                             ggggggTGTTTCCCCCCGGGCAAGCCCGGGGCGCCCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCAGCGTATTTTGCCAAGGAAAAGGGGGGCGGAGccc  <  1:1098421/100‑1 (MQ=21)                                                                                                     |                                                                                             CCCTCAGACGGCCCGCGCGTTTATAGGACGCTCCGGCTACCGAAGGAAGCGCGCCGGTGCAAGGGGGGAGGCGCAAAGGGTTTGAACGGTCGCGGGGGTGTTTCGCCTCGGGCAAGCCCGAGGCGACCCGCGGGGGGCTTCGCCCCCCGCACCCCCCCAGCGTATTTTGCCAAGGAAAAGGGGGGCGGAGCCC  >  NC_014034/390705‑390897 Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 21 ≤  ATCG  < 32 ≤  ATCG  < 39 ≤  ATCG