BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_014034	996,774	+AACCGCGGCGAGATCGCC	100%	coding (24/1986 nt)	pccA →	propionyl‑CoA carboxylase subunit alpha

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_014034	996768 =	0 (0.000)	219 (1.040)	119/178	0.2	0.95	coding (18/1986 nt)	pccA	propionyl‑CoA carboxylase subunit alpha
*	?	NC_014034	= 996774	22 (0.090)	219 (1.040)	119/178	0.2	0.95	coding (24/1986 nt)	pccA	propionyl‑CoA carboxylase subunit alpha

Only 100 of 496 total aligned reads displayed.


CGTTTCGTTTCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCCG  >  NC_014034/996668‑996868


ttgacggcacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgag                                                                                                      >  2:244305‑M1/101‑101 (MQ=255)


 tgacggcacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaga                                                                                                     <  2:1351997‑M1/2‑1 (MQ=255)


  gacggcacatgacatcacgaaggggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGAt                                                                                                    >  2:4045360‑M1/99‑101 (MQ=255)


      gtttcgCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGcc                                                                                                >  1:1502759/1‑101 (MQ=255)


      gcacatgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGcc                                                                                                <  1:677766‑M1/7‑1 (MQ=255)


        ttCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaa                                                                                              >  2:3055703‑M2/1‑99 (MQ=255)


          atgacatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGcc                                                                                            >  2:3440674‑M1/91‑101 (MQ=255)


           gcccgccTTGACGGGCCAGGACAGCACGAAGGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGccaaccg                                                                                           <  1:1891759/101‑6 (MQ=255)


           gcccgccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccg                                                                                           >  2:4206782‑M2/1‑96 (MQ=255)


           gcccgccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccg                                                                                           <  2:22228‑M2/101‑6 (MQ=255)


             acatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCcgcg                                                                                         <  2:4126288‑M1/14‑1 (MQ=255)


             acatcacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCcgcg                                                                                         <  1:1021039‑M1/14‑1 (MQ=255)


                ccTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcg                                                                                      >  1:570051‑M2/1‑91 (MQ=255)


                 cacgaaagggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGtcat                                                                                     <  1:2171089‑M1/18‑1 (MQ=255)


                 cTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcga                                                                                     <  1:1923562‑M2/101‑12 (MQ=255)


                   tGACGGCACATGACATCGCGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgaga                                                                                   >  1:1198131‑M2/1‑88 (MQ=255)


                   tGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgaga                                                                                   >  2:433099‑M2/1‑88 (MQ=255)


                    gACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagat                                                                                  >  1:4235492‑M2/1‑87 (MQ=255)


                    gACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagat                                                                                  >  1:4550780‑M2/1‑87 (MQ=255)


                      cGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcg                                                                                <  2:1094293‑M2/101‑17 (MQ=255)


                       agggggcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATc                                                                               <  1:1268383‑M1/24‑1 (MQ=255)


                         gaaatggCAGCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcct                                                                             >  1:2351791‑M2/4‑82 (MQ=255)


                         cacaTGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcct                                                                             >  1:2848447‑M2/1‑82 (MQ=255)


                          acaTGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctg                                                                            <  1:3444837‑M2/101‑21 (MQ=255)


                          acaTGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctg                                                                            <  1:909246‑M2/101‑21 (MQ=255)


                            gcgcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGgcgc                                                                          >  1:3483430‑M1/73‑101 (MQ=255)


                             tGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccg                                                                         <  2:3449848‑M2/101‑24 (MQ=255)


                              gcgggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGgcgcgc                                                                        <  1:2464407‑M1/31‑1 (MQ=255)


                                gggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccagccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCaa                                                                      <  2:1256620‑M1/33‑1 (MQ=255)


                                gggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCaa                                                                      <  1:4344961‑M1/33‑1 (MQ=255)


                                gggccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCaa                                                                      >  2:3641898‑M1/69‑101 (MQ=255)


                                   ccaatccgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGAt                                                                   >  1:258913‑M1/66‑101 (MQ=255)


                                     cGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatca                                                                 <  2:1481180‑M2/101‑32 (MQ=255)


                                      gAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaa                                                                >  1:476322‑M2/1‑69 (MQ=255)


                                         cgcgcccggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCAt                                                             >  2:1744048‑M1/60‑101 (MQ=255)


                                          gggggCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcg                                                            <  2:557227‑M2/101‑37 (MQ=255)


                                          gggggCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcg                                                            <  1:3848123‑M2/101‑37 (MQ=255)


                                               cggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGAc                                                       >  1:3405725‑M1/54‑101 (MQ=255)


                                               cggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGAc                                                       <  2:4099152‑M1/48‑1 (MQ=255)


                                                ggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACg                                                      <  1:3796284‑M1/49‑1 (MQ=255)


                                                ggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACg                                                      <  2:2251597‑M1/49‑1 (MQ=255)


                                                ggaggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACg                                                      >  2:49321‑M1/53‑101 (MQ=255)


                                                 cgGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgca                                                     <  2:2596305‑M2/101‑44 (MQ=255)


                                                  aggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGAcggg                                                    <  1:1420346‑M1/51‑2 (MQ=255)


                                                  aggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGt                                                    >  1:2314636‑M1/51‑101 (MQ=255)


                                                  aggaagggactgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGt                                                    <  1:53101‑M1/51‑1 (MQ=255)


                                                   ggCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaag                                                   >  2:1010946‑M2/1‑56 (MQ=255)


                                                      cAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatg                                                <  2:3807391‑M2/101‑49 (MQ=255)


                                                      cAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatg                                                <  2:3327807‑M2/101‑49 (MQ=255)


                                                      cAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatg                                                >  2:3260254‑M2/1‑53 (MQ=255)


                                                       aaTCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgg                                               <  1:688482‑M2/101‑50 (MQ=255)


                                                          actgcccacgttcaagaaaatccggatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCt                                            >  2:2178271‑M1/43‑101 (MQ=255)


                                                           ctgaccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTa                                           >  1:1003644‑M1/42‑101 (MQ=255)


                                                              gcCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaa                                        <  1:1677131‑M2/101‑57 (MQ=255)


                                                              accatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTc                                        <  2:4555844‑M1/63‑1 (MQ=255)


                                                               ccatgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTcc                                       >  2:3504645‑M1/38‑101 (MQ=255)


                                                                catgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccg                                      <  1:459166‑M1/65‑1 (MQ=255)


                                                                catgttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccg                                      >  1:3328056‑M1/37‑101 (MQ=255)


                                                                 cGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagac                                     >  1:4186079‑M2/1‑42 (MQ=255)


                                                                  ggaggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacg                                    <  1:2939782‑M2/101‑61 (MQ=255)


                                                                   gttcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTAttccgaag                                   >  2:61906‑M1/34‑99 (MQ=255)


                                                                   gaggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacgg                                   <  1:4414216‑M2/101‑62 (MQ=255)


                                                                    attgccgaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgc                                  <  2:1597262‑M1/69‑1 (MQ=255)


                                                                     tcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgcc                                 >  2:3847838‑M1/32‑101 (MQ=255)


                                                                     tcaagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgcc                                 >  1:4088351‑M1/32‑101 (MQ=255)


                                                                     ggaAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtg                                 >  2:372879‑M2/1‑38 (MQ=255)


                                                                       aagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgccga                               >  1:1299752‑M1/30‑101 (MQ=255)


                                                                       aagaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgccga                               <  1:3185320‑M1/72‑1 (MQ=255)


                                                                        agaaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTccgatgccgat                              <  1:3243001‑M1/73‑1 (MQ=255)


                                                                         gggACTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccg                             <  1:3513118‑M2/101‑68 (MQ=255)


                                                                          aaaatcctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCcgatgccgatgc                            <  1:4107291‑M1/75‑1 (MQ=255)


                                                                            aCTGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtct                          <  1:1443679‑M2/101‑71 (MQ=255)


                                                                              tcctgatcgccaaccgaggcaaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAg                        >  2:3686782‑M1/23‑101 (MQ=255)


                                                                              tGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctat                        <  2:905477‑M2/101‑73 (MQ=255)


                                                                              tGACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctat                        <  2:1623576‑M2/101‑73 (MQ=255)


                                                                               gACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctatt                       >  2:2741129‑M2/1‑28 (MQ=255)


                                                                               gACCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctatt                       >  1:395853‑M2/1‑28 (MQ=255)


                                                                                ctgatcgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGgc                      >  2:688420‑M1/21‑101 (MQ=255)


                                                                                aCCATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattc                      >  1:2237968‑M2/1‑27 (MQ=255)


                                                                                 tgatcgccacccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGgcg                     >  1:36930‑M1/20‑101 (MQ=255)


                                                                                 ccATGTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattcc                     >  2:2272112‑M2/1‑26 (MQ=255)


                                                                                  gatcgccaaccgcggcgaGATGGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGgcgc                    <  1:2651885‑M1/83‑1 (MQ=255)


                                                                                     gTTCAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatg                 >  1:1366856‑M2/1‑22 (MQ=255)


                                                                                     cgccaaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGc                 <  2:2395653‑M1/86‑1 (MQ=255)


                                                                                        cAAGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccg              <  1:1716247‑M2/101‑83 (MQ=255)


                                                                                         aaccgcggcgaGATCGCCTGCCTCGTCATAAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCAtgt             >  2:778267‑M1/12‑101 (MQ=255)


                                                                                         aaccgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCAtgt             <  2:2117038‑M1/90‑1 (MQ=255)


                                                                                          accgcggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCAtgtg            >  1:1221120‑M1/11‑101 (MQ=255)


                                                                                          aGAAAATCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgat            <  1:1167696‑M2/101‑85 (MQ=255)


                                                                                            aaaaTCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgc          <  2:4486496‑M2/101‑87 (MQ=255)


                                                                                             atatcCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagccggtggccgtctattccgatgccgatgcg         <  2:3546268‑M2/99‑88 (MQ=255)


                                                                                              cggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGaaaa        <  2:3881115‑M1/95‑1 (MQ=255)


                                                                                              cggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGaaaa        >  2:2939553‑M1/7‑101 (MQ=255)


                                                                                              aaTCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgc        <  2:3118764‑M2/101‑89 (MQ=255)


                                                                                               ggcgaGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAAt       >  1:617701‑M1/6‑101 (MQ=255)


                                                                                                tCCTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcag      >  1:2726524‑M2/1‑11 (MQ=255)


                                                                                                  cTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcaggc    >  2:4376129‑M2/1‑9 (MQ=255)


                                                                                                  cTGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcaggc    >  2:1448468‑M2/1‑9 (MQ=255)


                                                                                                   tGATCGCCaaccgcggcgagatcgcctgccgcgtcatcaaatcggcgcgcaagatgggcatcaagacggtggccgtctattccgatgccgatgcgcaggcg   <  2:1383917‑M2/101‑94 (MQ=255)


                                                                                                    gATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCcg  >  1:3596442/1‑101 (MQ=255)


CGTTTCGTTTCGCCCGCCTTGACGGCACATGACATCACGAAAGGGGGCGCGGGCCAATCCGCGCCCGGAGGAAGGGACTGACCATGTTCAAGAAAATCCTGATCGCCTGCCGCGTCATCAAATCGGCGCGCAAGATGGGCATCAAGACGGTGGCCGTCTATTCCGATGCCGATGCGCAGGCGCTGCATGTGAAAATGGCCG  >  NC_014034/996668‑996868

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG