Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,975	2	.→C	100.0%	2.3	3	intergenic (+248/‑257)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Rejected: E-value exceeds prediction threshold.
Reads supporting (aligned to +/- strand):  new base (0/3):  ref base (0/0):  total (0/3)

AGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTC  >  NC_014034/2185876‑2186050                                                                                                        |                                                                            agcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..c                                                                            <  1:2119360/101‑1 (MQ=255)  gcagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cc                                                                           <  1:1732596/101‑1 (MQ=255)   cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..ccc                                                                          <  2:2675189/101‑1 (MQ=255)   cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..ccc                                                                          >  2:2041401/1‑101 (MQ=255)   cagcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..ccc                                                                          >  1:828243/1‑101 (MQ=255)     gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..ccccc                                                                        >  1:1375967/1‑99 (MQ=37)     gcGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..ccccc                                                                        <  1:3682239/101‑3 (MQ=37)      cGGCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccg                                                                       <  1:314406/101‑4 (MQ=37)      cGGCTTCCCCCGAAGGCC.TGTTTTTGATGCCAAAAGAAACGCGGAACCGCCCCCTTTCACCTTGGCCCAAATTCCCCGGGGGTCC.GGGGGCGGAG..cccccg                                                                       <  2:1797309/101‑4 (MQ=18)       ggCTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccgg                                                                      >  1:4653522/1‑97 (MQ=25)         cTTGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccggcg                                                                    >  2:1270081/1‑95 (MQ=25)          ttGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccggcgg                                                                   >  1:2738581/1‑94 (MQ=25)           tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccggcggg                                                                  >  2:1105762/1‑93 (MQ=21)           tGCCCCGAAGGCCTTG.TTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccggcggg                                                                  >  1:3001391/1‑93 (MQ=21)           tGCCCCGAAGGCCTT.ATTTTGATGGCACAAGAAACGCCGAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..cccccggcggg                                                                  >  1:3373897/1‑93 (MQ=21)                                                ccgacccccccccTTCCCCCTTGGCTCAAATACCCCGGGGGTCC.GGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAgc                              <  1:4396627/92‑1 (MQ=2)                                                               tccctttGGCTCAAATACCCGGGGGGTGC.GGGGGCGGAGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACtt               <  2:4653221/96‑1 (MQ=2)                                                                             attccccGGGGGGCCGGGGGGCGGGGCCCCCGGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTc  <  2:1677032/97‑1 (MQ=9)                                                                                                        |                                                                            AGCAGCGGCTTGCCCCGAAGGCCTTGTTTTTGATGGCACAAGAAACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCC.GGGGGCGGAG..CCCGCCGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTC  >  NC_014034/2185876‑2186050 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG