Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,325,125 G→T 100% P351T (CCC→ACC)  dacC2 ← D‑alanyl‑D‑alanine carboxypeptidase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,325,1250G→T100.0% 687.6 200P351T (CCC→ACC) dacC2D‑alanyl‑D‑alanine carboxypeptidase
Reads supporting (aligned to +/- strand):  new base (97/101):  ref base (1/0):  total (99/101)

Only 100 of 226 total aligned reads displayed.

ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGC  >  NC_014034/1325025‑1325221
                                                                                                    |                                                                                                
  caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                <  2:2252350/101‑1 (MQ=255)
  caTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgc                                                                                                <  2:2602817/101‑1 (MQ=255)
   aTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgcg                                                                                               >  1:662411/1‑101 (MQ=255)
   aTCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgcg                                                                                               <  2:482578/101‑1 (MQ=255)
   aTCGGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgcg                                                                                               <  1:1613520/101‑1 (MQ=255)
    tCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTgcgc                                                                                              <  2:3041188/101‑1 (MQ=255)
    tCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGgcgc                                                                                              >  2:2107220/1‑101 (MQ=255)
          gggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccc                                                                                        >  1:4220405/1‑101 (MQ=255)
          gggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccc                                                                                        <  1:2939759/101‑1 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                       <  2:1201448/101‑1 (MQ=255)
           ggTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagcccca                                                                                       <  1:3470243/101‑1 (MQ=255)
              cTGCGCCAGAGGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagcc                                                                                    >  1:4031866/1‑101 (MQ=255)
              cTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagcc                                                                                    >  2:1226974/1‑101 (MQ=255)
              cTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagcc                                                                                    <  1:4579857/101‑1 (MQ=255)
              cTGCGCCAGAAGCCGGCCCGCGGCGGAGGGGAAGCGGACCAGGAAGCGGCCCCCTGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGcagccccagcc                                                                                    >  2:3848908/1‑101 (MQ=255)
                gcgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCagccccagcccc                                                                                  <  2:3617336/101‑1 (MQ=255)
                gcgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCagccccagcccc                                                                                  >  1:829856/1‑101 (MQ=255)
                 cgcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCg                                                                                 <  1:2761780/101‑1 (MQ=255)
                  gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                >  2:19112/1‑101 (MQ=255)
                  gcCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCgg                                                                                >  1:107363/1‑101 (MQ=255)
                  gcCAGAAGCCGGCCCGCGGCGGCGGGGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCGGCCACCAGCGGCACGGAGCGCAGCCCCAGCCCCgc                                                                                >  2:2651081/1‑100 (MQ=255)
                    cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                              <  2:857534/101‑1 (MQ=255)
                    cAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCa                                                                              <  1:1822547/101‑1 (MQ=255)
                     aGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCAc                                                                             <  2:1431841/101‑1 (MQ=255)
                       aaGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACgg                                                                           >  2:104656/1‑101 (MQ=255)
                          ccGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGa                                                                        <  2:4279789/101‑1 (MQ=255)
                          ccGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGa                                                                        <  1:3809764/101‑1 (MQ=255)
                          ccGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGAACGGAGCGCAGCCCCAGCCCCGGCACGGTGa                                                                        >  2:1495955/1‑101 (MQ=255)
                           cGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAt                                                                       >  2:1388371/1‑101 (MQ=255)
                            ggCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAtt                                                                      >  1:258792/1‑101 (MQ=255)
                             gCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGAttt                                                                     <  2:4241453/101‑1 (MQ=255)
                               ccGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTcc                                                                   <  2:4084643/101‑1 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                  >  2:3969569/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                  >  1:30986/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                  >  2:683705/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                  >  1:2881220/1‑101 (MQ=255)
                                cgcgGCGGCGGTGAACGGGGCCCGGAAGCCGGCCGCGGCGACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCa                                                                  <  2:535961/101‑1 (MQ=255)
                                 gcggcggcgGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAg                                                                 <  2:1427409/101‑1 (MQ=255)
                                   ggcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgcc                                                               >  2:1672087/1‑101 (MQ=255)
                                   ggcggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgcc                                                               <  1:1020523/101‑1 (MQ=255)
                                    cgggcggTGAGGCGGTCAAGAAAGCCGCCCTCGGGCAGGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgccg                                                              <  2:4467030/99‑1 (MQ=255)
                                      ggcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAgccgcc                                                            <  1:1842611/101‑1 (MQ=255)
                                       gcggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGccc                                                           >  1:3615401/1‑101 (MQ=255)
                                        cggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGcccc                                                          >  1:199763/1‑101 (MQ=255)
                                         ggTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCa                                                         <  1:1145636/101‑1 (MQ=255)
                                          gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                        >  1:291110/1‑101 (MQ=255)
                                          gTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAg                                                        >  2:1861334/1‑101 (MQ=255)
                                           tGAAGCGGACCAGGAAGCCCCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGc                                                       <  2:1600634/101‑1 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                     <  1:1395740/101‑1 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                     >  2:4423057/1‑101 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                     >  1:2673191/1‑101 (MQ=255)
                                             aaGCGGACCAGGAAGCCGCCCTCGGCCACGTCGATTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTc                                                     <  1:4001408/101‑1 (MQ=255)
                                              aGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCg                                                    >  1:3032993/1‑101 (MQ=255)
                                                  gACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGc                                                >  1:1536257/1‑101 (MQ=255)
                                                   aCCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCACCTCGGTGcc                                               >  1:2832769/1‑101 (MQ=255)
                                                   aCCAGGAAGCCGCCCTAGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGcc                                               >  2:442842/1‑101 (MQ=255)
                                                       ggAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgcc                                           <  1:2431944/101‑1 (MQ=255)
                                                       ggAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgcc                                           >  1:807120/1‑101 (MQ=255)
                                                        gAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCccgccg                                          <  2:2394517/101‑1 (MQ=255)
                                                          aGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTc                                        >  1:544938/1‑101 (MQ=255)
                                                           cccgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCa                                       <  2:4536416/100‑1 (MQ=255)
                                                            ccgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCAc                                      >  1:3132874/1‑101 (MQ=255)
                                                             cgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCAcc                                     <  1:524596/101‑1 (MQ=255)
                                                             cgccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCAcc                                     <  2:107349/101‑1 (MQ=255)
                                                              gccCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCg                                    >  1:670474/1‑101 (MQ=255)
                                                              ccccTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCg                                    <  2:1237605/100‑1 (MQ=255)
                                                                   cGGCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGcgcg                               <  2:4333434/101‑1 (MQ=255)
                                                                    ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                              >  1:4308677/1‑101 (MQ=255)
                                                                    ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                              >  1:193390/1‑101 (MQ=255)
                                                                    ggCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCgg                              >  2:201932/1‑101 (MQ=255)
                                                                     gCCCCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGt                             <  1:4423349/101‑1 (MQ=255)
                                                                     gCCACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGt                             <  1:4459304/101‑1 (MQ=255)
                                                                      ccACGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTg                            >  2:3935207/1‑101 (MQ=255)
                                                                       cccGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGa                           <  2:2344890/99‑1 (MQ=255)
                                                                        ccGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAc                          <  2:3715863/100‑1 (MQ=255)
                                                                        aCGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAc                          >  2:2483907/1‑101 (MQ=255)
                                                                         cGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAcc                         <  2:624146/101‑1 (MQ=255)
                                                                         cGTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGAcc                         >  1:1246388/1‑101 (MQ=255)
                                                                          gTCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCg                        >  1:4083625/1‑101 (MQ=255)
                                                                           tCGGTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCgg                       >  1:2139331/1‑101 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                    <  1:2194072/101‑1 (MQ=255)
                                                                              gTTTCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGcc                    >  2:2727543/1‑101 (MQ=255)
                                                                                 tCCGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTc                 >  2:1481734/1‑101 (MQ=255)
                                                                                  ccGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCa                >  1:3451980/1‑101 (MQ=255)
                                                                                   cGCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaa               <  2:807046/101‑1 (MQ=255)
                                                                                    gCCACCAGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCaaa              >  1:1374835/1‑101 (MQ=255)
                                                                                     cccccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg             <  2:291078/98‑1 (MQ=255)
                                                                                     ccaccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAg             >  2:46320/1‑101 (MQ=255)
                                                                                      cccagccGGCAGTGGGCGCACCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGa            <  2:4036615/95‑1 (MQ=255)
                                                                                        ccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc          <  2:3249632/101‑1 (MQ=255)
                                                                                        ccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc          <  1:2750353/101‑1 (MQ=255)
                                                                                        ccaGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc          >  1:471077/1‑101 (MQ=255)
                                                                                        ccaGCGGCACGGTGCGCAGCCACAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGAcc          >  2:3621708/1‑101 (MQ=255)
                                                                                          aGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGc        >  1:2695133/1‑101 (MQ=255)
                                                                                          aGCGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGc        >  2:3192314/1‑101 (MQ=255)
                                                                                            cGGCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt      <  2:199739/101‑1 (MQ=255)
                                                                                            cGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt      <  2:2452940/101‑1 (MQ=255)
                                                                                            cGGCACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCt      >  2:4179995/1‑101 (MQ=255)
                                                                                             ggCCCGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTc     <  2:1133830/101‑1 (MQ=255)
                                                                                               cACGGTGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCgg   >  1:3238819/1‑101 (MQ=255)
                                                                                                    |                                                                                                
ACCATCTGACGGGTCTGCGCCAGAAGCCGGCCCGCGGCGGCGGTGAAGCGGACCAGGAAGCCGCCCTCGGCCACGTCGGTTTCCGCCACCAGCGGCACGGGGCGCAGCCCCAGCCCCGGCACGGTGATTTCCAGCCGCCCCAGCTCGGTGCCCGCCGTCACCGGCGCGGTGACCGGGCCGTCAAAGACCGCCTCGGC  >  NC_014034/1325025‑1325221

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG