Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,968 G→C 10.4% G284R (GGT→CGT)  RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9680G→C10.4% 67.0 ‑0.7 0.0324G284R (GGT→CGT) RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (19/15):  ref base (142/147):  total (162/162)
Fisher's exact test strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068
                                                                                                               |                                                                                                       
  gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.Gt                                                                                                      <  1:2148566/101‑1 (MQ=255)
   tCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTc                                                                                                     >  2:2393811/1‑101 (MQ=255)
    ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCg                                                                                                    <  1:3079118/101‑1 (MQ=255)
     cAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGc                                                                                                   >  2:3595621/1‑101 (MQ=255)
       gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTc                                                                                                 <  1:527195/101‑1 (MQ=255)
       gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTc                                                                                                 >  2:2317944/1‑101 (MQ=255)
        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcc                                                                                                >  2:2047632/1‑101 (MQ=255)
            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcccccc                                                                                            <  2:1248073/101‑1 (MQ=255)
            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTcccccc                                                                                            >  1:2023556/1‑101 (MQ=255)
             gtccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCACCCcg                                                                                           <  2:944517/99‑1 (MQ=255)
               ccGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCCCCCCCcgcg                                                                                         >  1:351994/1‑101 (MQ=255)
                 gcgcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggg                                                                                       <  2:3347745/101‑1 (MQ=255)
                   gcgGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCggggg                                                                                     <  1:2271012/101‑1 (MQ=255)
                    cgtaCCTCCTCAGACGGCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGc                                                                                    <  2:4145228/98‑1 (MQ=255)
                        cctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                <  2:841411/101‑1 (MQ=255)
                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                                                                >  1:1780407/11‑101 (MQ=9)
                         attggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                               >  1:2704551/11‑101 (MQ=0)
                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  1:2113141/101‑1 (MQ=255)
                          cgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGa                                                                               <  2:2701087/93‑1 (MQ=2)
                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TGAG.CTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             >  2:3650505/9‑101 (MQ=2)
                           tgccccccGTCCCTCCCCGCGCGGGGGCGTGGATCGAAGCA..CCT.GTCC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                                                             <  2:3133455/94‑1 (MQ=2)
                           gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  2:1135072/94‑1 (MQ=2)
                           gcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGAt                                                                              <  2:685449/94‑1 (MQ=2)
                            ctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:2797965/101‑1 (MQ=255)
                             ggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGGTCg                                                                            >  2:439236/6‑101 (MQ=2)
                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:4392637/95‑1 (MQ=9)
                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  1:3482088/1‑101 (MQ=255)
                              acactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                                                           >  2:178334/6‑101 (MQ=9)
                               ttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  2:2149569/4‑101 (MQ=2)
                               ggtggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.GC.AGCG.AGGGCG.TCTGG.A.ACA..GGAT.GTCGCTCCCCCCGCCGGGGCGTGGAtggga                                                                          >  1:3690788/5‑97 (MQ=0)
                               gcccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:163732/98‑1 (MQ=2)
                               ccccGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                            <  1:2345228/98‑1 (MQ=2)
                               cactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                                                          >  1:1808681/5‑101 (MQ=9)
                               aggcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                         <  1:1347308/98‑1 (MQ=255)
                               aGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCCCCGTGGCGTGGAGCGgaa                                                                         >  2:3964688/1‑101 (MQ=37)
                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                                                       >  2:1200031/3‑100 (MQ=2)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCCCAACac                                                                      >  2:3204231/1‑100 (MQ=2)
                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcg                                                                     <  2:4612903/101‑4 (MQ=9)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGCGGATCGAAACac                                                                      >  1:2232993/1‑100 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCGCCGCGGGGGCGTGGATCGAAAcccgc                                                                    >  1:3919459/1‑97 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                                   >  1:3830550/1‑96 (MQ=2)
                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacac                                                                   >  2:4555217/1‑96 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgcc                                                                   <  1:2911496/101‑6 (MQ=2)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGGTCCAAAcccggc                                                                   >  1:869624/1‑96 (MQ=9)
                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                                                   >  1:974881/1‑96 (MQ=9)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                                                    >  1:2046855/1‑101 (MQ=2)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCt                                                                 <  1:4251013/101‑1 (MQ=255)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaa                                                                   >  2:3443831/1‑100 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccac                                                                 >  2:1017286/1‑94 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                                >  2:2429733/1‑93 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccgg                                                                >  1:250446/1‑93 (MQ=2)
                                        tCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCGC.CGCG.CCGGATCTT.TG.T.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggcct                                                                 >  1:2581699/1‑94 (MQ=9)
                                        cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                                <  1:3017310/100‑1 (MQ=255)
                                        cccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtc                                                                <  1:4602979/100‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                                                  <  2:481374/101‑3 (MQ=2)
                                         ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.GC.AGTG.TCAGGT.T..TT.C.CAG..CGGC.GTCGCTCCCCCCCCCGGGGCGTGGATCGGAAcccgccacg                                                                >  2:1574265/1‑93 (MQ=0)
                                         ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                               <  1:1500287/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACA.GCCT.GCACG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg                                                               <  1:4008062/101‑1 (MQ=255)
                                         ccccccGCGGGGGCGTGGATCGAAACA..ACT.GACC...C.GGCA.ACGGCC.TA.TG.CGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacaccggg                                                               <  1:4485809/101‑10 (MQ=2)
                                          cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcct                                                              >  1:4527915/1‑99 (MQ=255)
                                           ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                                                                <  2:2927158/101‑5 (MQ=2)
                                           ccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcg.tc                                                            >  1:722427/1‑101 (MQ=255)
                                             ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.CCATGAATTCATCG.AG.CG.C.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                                                              <  1:579055/101‑7 (MQ=0)
                                             ccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcg.tcgt                                                          >  1:4200435/1‑101 (MQ=255)
                                              cgggggggCGTGCATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGtt                                                         <  2:1426817/98‑1 (MQ=255)
                                                cgGGGGGGTGGATCGAAACA.GCCT.GCCCG.GN.CGCA.TCGGCT.TC.CT.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTgg                                                       <  1:1231237/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGCGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGt                                                      >  1:3965679/1‑101 (MQ=255)
                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                 >  1:2408764/1‑101 (MQ=255)
                                                      cGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGcc                                                 >  2:4261865/1‑101 (MQ=255)
                                                        tGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCgg                                               >  2:1862839/1‑101 (MQ=255)
                                                         ggATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGa                                              <  2:3776703/101‑1 (MQ=255)
                                                          gATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAc                                             <  1:3735938/101‑1 (MQ=255)
                                                           aTCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.g                                           <  1:2675446/101‑1 (MQ=255)
                                                               aaaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCTCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGt                                       >  1:4331037/1‑101 (MQ=255)
                                                                aaCA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTc                                      >  2:3023395/1‑101 (MQ=255)
                                                                  cA.GCGT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGGGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCg                                    <  2:2985757/101‑1 (MQ=255)
                                                                       cT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGGTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGc                                >  2:2538739/1‑101 (MQ=255)
                                                                        t.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCt                               >  1:809899/1‑101 (MQ=255)
                                                                           cgcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTcc                             >  2:1320461/1‑101 (MQ=255)
                                                                            gcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTccc                            <  2:2483171/101‑1 (MQ=255)
                                                                            gcg.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTccc                            >  1:1461669/1‑101 (MQ=255)
                                                                                    gCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCcgcg                      <  2:756917/101‑1 (MQ=255)
                                                                                      a.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCggg                    >  2:4460178/1‑101 (MQ=255)
                                                                                         cgccT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCCGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCggggg                  <  2:1688783/98‑1 (MQ=255)
                                                                                         cGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTTGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCggggg                  <  2:1096522/101‑1 (MQ=255)
                                                                                          ggCT.TC.CG.C.CTG..CGTG.GTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGc                 <  2:2023416/101‑1 (MQ=255)
                                                                                            cT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGt               <  2:2244564/101‑1 (MQ=255)
                                                                                             t.tC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCTCCCGCGGGGGCGTg              <  1:1046935/101‑1 (MQ=255)
                                                                                             t.tC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCggg              >  2:1386681/1‑99 (MQ=255)
                                                                                                   g.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATc          >  2:1954706/1‑101 (MQ=255)
                                                                                                     c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         >  1:3159592/1‑101 (MQ=255)
                                                                                                     c.cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         <  2:3512213/101‑1 (MQ=255)
                                                                                                       cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa        >  1:2458862/1‑101 (MQ=255)
                                                                                                       cTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa        >  2:4094920/1‑101 (MQ=255)
                                                                                                          ccgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCg         <  2:2653565/96‑1 (MQ=2)
                                                                                                           cgccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGa        >  2:3724353/5‑101 (MQ=2)
                                                                                                             ccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa      >  1:3243559/3‑101 (MQ=2)
                                                                                                             ccg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa      <  1:1034040/99‑1 (MQ=2)
                                                                                                              gg.gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg   <  1:1955651/100‑1 (MQ=255)
                                                                                                              cg.gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTTGACCGCATCGAAGCTGGTCACATCGCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc     <  1:1883622/100‑1 (MQ=2)
                                                                                                               |                                                                                                       
CGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.GC.CGCA.TCGGCT.TC.CG.C.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCG.TCGTTGGTCAGCCGGAC.GATGTCCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGC  >  NC_014034/1396868‑1397068

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG