Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,668,994 C→T 100% S390N (AGC→AAC)  ilvI ← acetolactate synthase large subunit

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,668,9940C→T100.0% 829.3 211S390N (AGC→AAC) ilvIacetolactate synthase large subunit
Reads supporting (aligned to +/- strand):  new base (113/98):  ref base (0/0):  total (113/98)

Only 100 of 252 total aligned reads displayed.

CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCC  >  NC_014034/1668896‑1669090
                                                                                                  |                                                                                                
ccAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGGGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTg                                                                                                <  1:1679508/101‑1 (MQ=255)
     ccTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGccc                                                                                           <  2:2109795/101‑1 (MQ=255)
       tCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCt                                                                                         <  2:3265231/101‑1 (MQ=255)
        ccGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtt                                                                                        >  2:1711048/1‑101 (MQ=255)
          gCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTgg                                                                                      >  1:3042971/1‑101 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCtagatc                                                                                    >  2:3960448/1‑96 (MQ=255)
            tGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTc                                                                                    <  1:800766/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCTCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCa                                                                                   <  2:4005647/101‑1 (MQ=255)
             ggTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCa                                                                                   >  1:2000517/1‑101 (MQ=255)
                cATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAggg                                                                                <  1:1921222/101‑1 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                               <  2:713997/101‑1 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                               >  2:1115040/1‑101 (MQ=255)
                 aTCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGc                                                                               >  1:2189237/1‑101 (MQ=255)
                    cAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                            <  1:687777/101‑2 (MQ=255)
                    cAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                            <  2:574344/101‑2 (MQ=255)
                    cAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtc                                                                            <  1:4409278/101‑2 (MQ=255)
                     aGCGGTTCGGCGACTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCtcc                                                                           >  1:2530020/1‑99 (MQ=255)
                      gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                          <  1:1788187/101‑1 (MQ=255)
                      gCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCACCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                          <  1:4004439/101‑1 (MQ=255)
                      gCGGCTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCa                                                                          <  1:4196987/101‑1 (MQ=255)
                       cGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAg                                                                         <  2:2052390/101‑1 (MQ=255)
                         gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                       >  1:2565904/1‑101 (MQ=255)
                         gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                       <  1:4330406/101‑1 (MQ=255)
                         gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                       <  2:4201539/101‑1 (MQ=255)
                         gTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGcc                                                                       <  2:2640236/101‑1 (MQ=255)
                           tCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGc                                                                     >  1:1539809/1‑101 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGGGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCCCCAGCCGCt                                                                    >  2:10706/1‑101 (MQ=255)
                            cGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCt                                                                    <  1:3516308/101‑1 (MQ=255)
                              gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                  <  1:3849720/101‑1 (MQ=255)
                              gcgcCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTcc                                                                  <  2:2842089/101‑1 (MQ=255)
                                  cTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                              >  1:2621778/1‑101 (MQ=255)
                                  cTCGAAGCCCAGATATTGCGCCGCCCACATATGATGCTGGCCGACTTCGGTGGTGCTGTGGTGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcg                                                              <  2:3837353/101‑1 (MQ=255)
                                   tCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAgcgc                                                             >  1:120184/1‑101 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                           <  2:2290059/101‑1 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                           >  2:685685/1‑101 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                           >  1:2672868/1‑101 (MQ=255)
                                     gAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCat                                                           >  1:1568162/1‑101 (MQ=255)
                                      aaGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCata                                                          <  1:4371057/101‑1 (MQ=255)
                                        gCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATAtt                                                        <  2:2189087/101‑1 (MQ=255)
                                          ccAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGc                                                      >  2:903844/1‑101 (MQ=255)
                                           cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                     >  2:3404082/1‑101 (MQ=255)
                                           cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                     >  1:2067120/1‑101 (MQ=255)
                                           cAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCg                                                     <  2:100214/101‑1 (MQ=255)
                                             gATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGt                                                   >  1:4038219/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                  >  2:3506737/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                  >  1:4544598/1‑101 (MQ=255)
                                              atatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGtt                                                  >  1:1946694/1‑101 (MQ=255)
                                               tatTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGttt                                                 <  2:1447642/101‑1 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                >  1:1007993/1‑101 (MQ=255)
                                                atTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTg                                                <  1:2461770/101‑1 (MQ=255)
                                                 ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                               <  1:3620360/101‑2 (MQ=255)
                                                 ttGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTga                                               <  1:4143892/101‑2 (MQ=255)
                                                   gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                             <  1:4494948/101‑1 (MQ=255)
                                                   gcgcCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATg                                             >  2:3616696/1‑101 (MQ=255)
                                                      ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                          <  2:1174870/101‑1 (MQ=255)
                                                      ccgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATg                                          <  2:3276167/101‑1 (MQ=255)
                                                       cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                         <  2:798752/101‑1 (MQ=255)
                                                       cgccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGc                                         >  1:1500272/1‑101 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                        <  2:1054726/101‑1 (MQ=255)
                                                        gccCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCt                                        <  1:4671500/101‑1 (MQ=255)
                                                          ccACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGt                                      >  2:567722/1‑101 (MQ=255)
                                                            ccaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                    <  2:1649831/100‑1 (MQ=255)
                                                            acaTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTcc                                    <  1:31952/101‑1 (MQ=255)
                                                              aTCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGa                                  <  2:1125090/101‑1 (MQ=255)
                                                                cTGATGGTGGCCGACTTGGGTGGTGATGTAGCTGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGc                                <  2:442628/101‑1 (MQ=255)
                                                                 tGATGCTGGCCGACTTCGGTGGGGATGTGCGGGTTGCCCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGcc                               <  2:4637225/101‑1 (MQ=255)
                                                                  gATGCTGGCCGACTTCGGTGGTGATGTAGCGTTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCg                              <  1:1856137/101‑1 (MQ=255)
                                                                   aTGCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCgg                             <  1:740826/101‑1 (MQ=255)
                                                                     gCTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtg                           >  1:2844759/1‑101 (MQ=255)
                                                                      cTGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGgtgt                          >  2:1910574/1‑101 (MQ=255)
                                                                       tGGCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                         >  2:1838994/1‑101 (MQ=255)
                                                                       gggCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTa                         <  2:3219888/100‑1 (MQ=255)
                                                                         gcggccTTCGGGGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                       <  2:1328338/96‑1 (MQ=255)
                                                                         gCCGACTTCGGTGGTGATGTAGCGTTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                       <  1:2452242/101‑1 (MQ=255)
                                                                         gCCGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAgg                       <  2:354813/101‑1 (MQ=255)
                                                                           cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                     >  2:4441584/1‑101 (MQ=255)
                                                                           cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                     >  2:4337544/1‑101 (MQ=255)
                                                                           cGACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCg                     >  2:4133825/1‑101 (MQ=255)
                                                                            gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                    >  2:724696/1‑101 (MQ=255)
                                                                            gACTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGa                    >  2:2369785/1‑101 (MQ=255)
                                                                             ccTTCGGTGGTGATGTGCCGGTTGCCCCCCTGGGTCGGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                   <  2:3029864/100‑1 (MQ=255)
                                                                             aCTTCGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                   >  1:3157166/1‑101 (MQ=255)
                                                                             aCTTCGGGGGTGATGTAGCGGTTGCGCCCTTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAg                   <  2:4544303/101‑1 (MQ=255)
                                                                                 gggtggtGATGTAGCGGTTGCCCCCCTTGGTCGGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               <  1:3904077/100‑1 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               <  2:1568059/101‑1 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               <  1:2250601/101‑1 (MQ=255)
                                                                                 cGGTGGTGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAg               >  2:2764795/1‑101 (MQ=255)
                                                                                  ggtggtGATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCAGGTGTAGGCGAGGCAGc              >  2:4194432/1‑101 (MQ=255)
                                                                                   gtggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCg             >  1:4293781/1‑101 (MQ=255)
                                                                                    tgttgattaaGCGGTTCCGCCCCTTGGGCGGGGCCGCCAGCCGCTCCAGCGAATATTGCGGTTTGATGATTCTCTCCGAGCCGGTGTAGGCGAGGCAGCgg            <  1:714201/92‑1 (MQ=255)
                                                                                    tggtgATGTAGCGGTTGCGCCCCTTGGTCAGGGCATCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCgg            <  1:1424122/101‑1 (MQ=255)
                                                                                      gtgATGTAGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAt          <  1:3098146/101‑1 (MQ=255)
                                                                                       tgttGTGGCGGTTGCGCCCCTTGGTCAGGCCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc         <  1:520884/98‑1 (MQ=255)
                                                                                       tgATGTGGCGGTTCCGCCCCTTGGTCCGCCCCCCCAGCCGCCCCCGCCCATATTGCGTTTTGATGATGCTGGCCGAGCCGGTGTAGGCGAGGCAGCGGAtc         <  1:10711/101‑1 (MQ=255)
                                                                                       tgATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtc         >  2:2610378/1‑101 (MQ=255)
                                                                                        gATGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGGAGGCGAGGCAGCGGAtct        >  2:4283898/1‑101 (MQ=255)
                                                                                         aTGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtctt       >  1:2704063/1‑101 (MQ=255)
                                                                                          tGTAGCGGTTGCGCCCCTTGGTCAGGGCCTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCAGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc      >  2:3068745/1‑101 (MQ=255)
                                                                                          tGTAGCGGTTGCCCCCCTGGGCCGGGCCCCCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttc      <  1:3600406/101‑1 (MQ=255)
                                                                                           gTAGCGGTTGCGCCCCTTGGTCAGGGACTCCAGCCGCTCCAGCGCATATTGCGGTTTGATGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGAtcttct     >  2:2331963/1‑101 (MQ=255)
                                                                                                  |                                                                                                
CCAGCCCTCCGCTGGTCATCCAGCGGTTCGGCGCCTCGAAGCCCAGATATTGCGCCGCCCACATCTGATGCTGGCCGACTTCGGTGGTGATGTAGCGGCTGCGCCCCTTGGTCAGGGCCTTCAGCCGCTCCAGCGCATATTGCGGTTTGGTGATGCTGTCCGAGCCGGTGTAGGCGAGGCAGCGGATCTTCTTCC  >  NC_014034/1668896‑1669090

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG