Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,734,838 G→T 3.5% intergenic (‑91/+102) RCAP_rcc01603 ← / ← RCAP_rcc01604 hemolysin‑III family protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,734,8380G→T 3.5% 8.0 ‑1.4 ‑0.0194intergenic (‑91/+102)RCAP_rcc01603/RCAP_rcc01604hemolysin‑III family protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (5/2):  ref base (81/106):  total (86/108)
Fisher's exact test strand distribution p-value = 2.45e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.69e-01

Only 100 of 202 total aligned reads displayed.

GTCGTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734738‑1734936
                                                                                                    |                                                                                                        
gTCGTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGgcg                                                                                                          >  2:4027798/1‑101 (MQ=255)
 tCGTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGgcgc                                                                                                         >  1:4619201/1‑101 (MQ=255)
   gtgtCATCGAACCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.g                                                                                                      <  2:241349/101‑1 (MQ=255)
   gtgtCATCGAAACCTCCGGGGAATGGCGCTTCCAAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.g                                                                                                      <  2:1151991/101‑1 (MQ=255)
   gtgtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCACGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGGAGGGGGGCGCT.g                                                                                                      >  1:1835025/1‑101 (MQ=255)
     gtCATCGAACCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.cc                                                                                                   <  2:4621137/101‑1 (MQ=255)
     gtCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.cc                                                                                                   <  1:2639638/101‑1 (MQ=255)
        aTCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGGGCT.G.ccc.cc                                                                                               >  2:824162/1‑101 (MQ=255)
        aTCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.ccc.cc                                                                                               <  1:4042874/101‑1 (MQ=255)
          cGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.ccc.cccg                                                                                             >  2:3070859/1‑99 (MQ=255)
            aaaCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGGGCG.G.cgc.cccccc                                                                                           >  2:1232062/1‑97 (MQ=255)
            aaaCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.ccc.cccgtc                                                                                           >  1:3787941/1‑97 (MQ=255)
            aaaCCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.ccc.cccgtc                                                                                           >  2:2334089/1‑97 (MQ=255)
               ccTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTg                                                                                         <  2:3720044/101‑1 (MQ=255)
                cTCGGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGc                                                                                        <  2:260627/101‑1 (MQ=255)
                cTCCGGGGAAGGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGc                                                                                        <  2:1619480/101‑1 (MQ=255)
                  ccGGGAAATGGCGCATCCTAACAGCCGGGGCGGGCAAGCCAAAAGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCATGCgg                                                                                      <  1:318334/101‑1 (MQ=37)
                    ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                    >  2:1021212/1‑101 (MQ=255)
                    ggggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGAc                                                                                    <  2:2230495/101‑1 (MQ=255)
                     gggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACt                                                                                   >  1:1201491/1‑101 (MQ=255)
                      ggAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTc                                                                                  <  2:2547543/101‑1 (MQ=255)
                           ggCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTcccccc                                                                             >  1:2553774/1‑101 (MQ=255)
                           ggCGCAGCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTcccccc                                                                             <  1:1754898/101‑1 (MQ=255)
                           ggCCCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTcccccc                                                                             <  2:1436771/101‑1 (MQ=255)
                              gcATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCggg                                                                          <  2:3905654/101‑1 (MQ=255)
                                 tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGata                                                                       <  2:1457478/101‑1 (MQ=255)
                                 tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGata                                                                       <  1:3574375/101‑1 (MQ=255)
                                 tCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGata                                                                       >  1:80430/1‑101 (MQ=255)
                                  ccTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCgggggat                                                                      >  1:1640963/1‑97 (MQ=255)
                                    tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGGTAttt                                                                    >  2:3520758/1‑101 (MQ=255)
                                    tAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                    <  2:3580478/101‑1 (MQ=255)
                                    caggcgcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCCTCCGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATAttt                                                                    <  2:616689/96‑1 (MQ=18)
                                      gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                  <  1:2365798/101‑1 (MQ=255)
                                      gcagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTAt                                                                  >  1:416197/1‑101 (MQ=255)
                                       cagcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATg                                                                 <  2:3397913/101‑1 (MQ=255)
                                        agcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATgg                                                                <  1:117366/101‑1 (MQ=255)
                                         gcCGGGGCGGGCAAGCCAAGGGCTGACGCTGGGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                               <  1:2462901/101‑1 (MQ=21)
                                         gcCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGc                                                               <  2:1882044/101‑1 (MQ=255)
                                           cGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCaa                                                             >  2:3336391/1‑101 (MQ=255)
                                            ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                            >  1:4111430/1‑101 (MQ=255)
                                            ggggCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAg                                                            >  1:2277184/1‑101 (MQ=255)
                                             gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.a                                                          <  1:2761632/101‑1 (MQ=255)
                                             gggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.a                                                          <  1:2419850/101‑1 (MQ=255)
                                              ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGGAAG.At                                                         >  1:1163249/1‑101 (MQ=255)
                                              ggcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCCTCCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.At                                                         <  2:752858/101‑1 (MQ=255)
                                               gcgggcTAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATg                                                        <  1:765635/101‑1 (MQ=255)
                                               gcgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATg                                                        <  1:3516638/101‑1 (MQ=255)
                                                cgggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGa                                                       <  2:3657040/101‑1 (MQ=255)
                                                 gggcAAGCCAAAGGCTGTCGGTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGaa                                                      <  1:1971221/101‑1 (MQ=18)
                                                 gggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGaa                                                      <  1:3776779/101‑1 (MQ=255)
                                                  ggcAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGaaa                                                     >  2:681505/1‑101 (MQ=255)
                                                     aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTg                                                  <  2:1980975/101‑1 (MQ=255)
                                                     aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTg                                                  >  2:1070973/1‑101 (MQ=255)
                                                     aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTg                                                  >  2:4229995/1‑101 (MQ=255)
                                                     aaGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGAAATTTATTGGAAG.AAGaaaacg                                                  >  1:1604283/1‑98 (MQ=18)
                                                       gCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.g                                               <  1:1580250/101‑1 (MQ=255)
                                                        ccAATGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.gg                                              <  2:2930853/101‑1 (MQ=255)
                                                          aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                            >  2:2059384/1‑101 (MQ=255)
                                                          aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                            >  2:197414/1‑101 (MQ=255)
                                                          aaaGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGcc                                            >  1:4395081/1‑101 (MQ=255)
                                                             ggCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCCGGGCAATTATGGCAAGAATAAAATGGC.GGCCtc                                          >  1:5823/1‑100 (MQ=17)
                                                              gCTTACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCa                                        <  2:910693/101‑1 (MQ=255)
                                                              gCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCa                                        <  1:1068916/101‑1 (MQ=255)
                                                                  aCGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCTCC.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCtt                                    <  1:3938117/101‑1 (MQ=255)
                                                                   cGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTg                                   >  1:712115/1‑101 (MQ=255)
                                                                    gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                  <  2:1366481/101‑1 (MQ=255)
                                                                    gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                  >  2:4465189/1‑101 (MQ=255)
                                                                    gCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGc                                  <  1:3065265/101‑1 (MQ=255)
                                                                     cTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGcc                                 >  1:662959/1‑101 (MQ=255)
                                                                       gCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCAt                               <  1:1548212/101‑1 (MQ=255)
                                                                        cGTCAGCCGGCGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                              <  2:456167/101‑1 (MQ=255)
                                                                        cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGTGAAATTTATGGCAAG.ATGAAACTGC.GACCTTCATCTTGCCATa                              >  1:436418/1‑101 (MQ=17)
                                                                        cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                              >  1:474469/1‑101 (MQ=255)
                                                                        cGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATa                              >  1:2294734/1‑101 (MQ=255)
                                                                         gTCAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATaa                             >  2:1097034/1‑101 (MQ=255)
                                                                           cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                           >  2:2509379/1‑101 (MQ=255)
                                                                           cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAat                           <  1:3849642/101‑1 (MQ=255)
                                                                           cAGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGA.GGCCTTCATATTGCCATAAat                           >  2:4527879/1‑101 (MQ=17)
                                                                            aGCCGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAata                          >  2:2922739/1‑101 (MQ=255)
                                                                               cGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       <  2:372355/101‑1 (MQ=255)
                                                                               cGACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATcc                       >  2:4407014/1‑101 (MQ=255)
                                                                                gACGGGCCGGGCAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTCCATCTTGCCATAAATATccc                      >  1:295962/1‑101 (MQ=255)
                                                                                 gcgggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.g                    <  2:557275/100‑1 (MQ=255)
                                                                                  cgggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.gg                   >  1:2105841/1‑101 (MQ=255)
                                                                                   gggccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATTCC.ggg                  >  2:35097/1‑101 (MQ=255)
                                                                                     tgacggacggggggCTCT.G.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTg               >  2:18019/9‑101 (MQ=2)
                                                                                     gccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGt                <  1:848624/101‑1 (MQ=255)
                                                                                      ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGGAAG.ATGAAACTGC.GGGCTTCATCTTGCCCTAAATATCCC.cggggg               >  1:1745823/1‑99 (MQ=14)
                                                                                      ccgggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTg               <  1:1378546/101‑1 (MQ=255)
                                                                                       acggacggggggCTCT.G.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa             >  2:923127/7‑99 (MQ=2)
                                                                                       acggacggggggCTCT.G.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa             <  2:4332654/95‑3 (MQ=2)
                                                                                       acggacggggggCTCT.G.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaa             >  1:4440965/7‑99 (MQ=2)
                                                                                        gggcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCg             >  2:2106101/1‑101 (MQ=255)
                                                                                          gcAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCggg           <  2:3891332/101‑1 (MQ=255)
                                                                                          gacggggggCGCT.G.CCC.CCCGGCTTCG..CTCCCCCCGGGATATTTGCGGCAAG.ATGAAGGGGCATGATTTCATCTTGCCCGAAATATCCCAGGGGTgaattg          <  1:3012753/98‑6 (MQ=9)
                                                                                           cAGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCgggg          >  1:4273313/1‑101 (MQ=255)
                                                                                            aGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc         >  2:4078406/1‑101 (MQ=255)
                                                                                            aGGGGGGCGCT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGc         <  2:1640858/101‑1 (MQ=255)
                                                                                                  gcgcT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGcc   >  1:3890433/1‑101 (MQ=255)
                                                                                                   cgcT.GCCCC.CCGTCCTGCGGACTCCCCCCGGGATAGTTATAGTAAG.CTGCAACTAG.CGCCCTCAACTTGCCCTGAATATGCC.GGGGGGTGGGGTAGAGccc  >  2:4166224/1‑101 (MQ=2)
                                                                                                    |                                                                                                        
GTCGTGTCATCGAAACCTCCGGGGAATGGCGCATCCTAGCAGCCGGGGCGGGCAAGCCAAAGGCTGACGCTGCGTCAGCCGACGGGCCGGGCAGGGGGGCGCT.G.CCC.CCGTCCTGCGGACTCCCCCCGGGATATTTATGGCAAG.ATGAAACTGC.GGCCTTCATCTTGCCATAAATATCCC.GGGGTGCGGGGCAGAGCCC  >  NC_014034/1734738‑1734936

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG