BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	1,867,833	G→C	7.6%	intergenic (‑235/+80)	RCAP_rcc01729 ← / ← RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	1,867,833	0	G→C	7.6%	6.0 ‑3.7 ‑1.4	73	intergenic (‑235/+80)	RCAP_rcc01729/RCAP_rcc01730	pyridine nucleotide‑disulfide oxidoreductase/U32 family peptidase
Reads supporting (aligned to +/- strand): new base (0/6): ref base (35/32): total (35/38)
Fisher's exact test strand distribution p-value = 2.58e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.28e-02

Only 100 of 162 total aligned reads displayed.


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.G.CC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867733‑1867927


gAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCCCCCCCCGCGGCGCGCAAg.c.g                                                                                                       >  1:513516/1‑101 (MQ=37)


 aaaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAg.c.g.c                                                                                                     >  2:3555540/1‑101 (MQ=255)


  aaaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGCAAA.c.g.cc                                                                                                    >  2:3356950/1‑101 (MQ=38)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.c.g.ccc                                                                                                   <  2:4121574/101‑2 (MQ=255)


   aaGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.c.g.ccc                                                                                                   <  2:1440002/101‑2 (MQ=255)


    aGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.c.g.cccg                                                                                                  <  1:2379720/101‑3 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.c.g.cccgcc                                                                                                <  2:3898069/101‑5 (MQ=255)


      cACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.c.g.cccgcc                                                                                                <  1:4314862/101‑5 (MQ=255)


       aCTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGAAAC.c.g.cccgcca                                                                                               >  2:1891907/1‑96 (MQ=37)


        cTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGAGCCCCACCCCCCCGGCGCGAAAG.C.g.cc.ccccac                                                                                             >  1:2063997/1‑98 (MQ=25)


        cTGCTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCAt                                                                                              <  2:3119547/101‑1 (MQ=255)


         ttcTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGCCCGCACCCCC.AA.GCGC.GCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATg                                                                                             <  2:4117935/99‑1 (MQ=25)


          gCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCCCCCCCCGCGGCGCGAAAG.c.g.cccgcccagg                                                                                            >  1:4487493/1‑93 (MQ=25)


          gCTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGCCCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATgg                                                                                            <  1:2897925/101‑1 (MQ=25)


           cTTTCCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATggg                                                                                           <  2:1646922/101‑1 (MQ=255)


                ccccGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.A.GCCC.GCCATgggcgggg                                                                                      <  1:426477/101‑1 (MQ=255)


                   cgcccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCCCCCAGCCCCGGGGGCGCGAAAG.C.GCCC.GCCATGggcggggcgg                                                                                   <  1:4187854/101‑1 (MQ=21)


                    cccggtgtCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGgcggggcggg                                                                                  <  2:1958206/97‑1 (MQ=255)


                     cccgGGTCGCGACAGCGTTGCAAAGCCCGCACCCCC.AG.GCGC.GCGAGAGGCCAGCCCCCAGCCCCGGCGGCGAGAAAG.C.CCCC.GCCATGGGCGgggcgggc                                                                                 <  1:2440504/101‑1 (MQ=18)


                     cccgGGTCGCGACAGCGTGGCAAAGACCGCACCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGgggcgggc                                                                                 <  1:3382624/101‑1 (MQ=18)


                      ccgTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACCCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCCTGGgcgggggggggg                                                                                >  2:1211715/1‑99 (MQ=21)


                       cgTGTCGCGACAGCGTTGCAAAGCCCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGgcgc                                                                               <  1:3357199/101‑1 (MQ=255)


                       cgTGTCGCGACAGCGTTGCAAAGACCGATTCCCA.AA.GCCC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.CCCC.GCCATGGGCGGGGCGGgcgc                                                                               <  2:337577/101‑1 (MQ=18)


                        gtgtCGCGACAGCGTTGCAAAGCCCGCACCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.t                                                                             <  1:76339/101‑1 (MQ=18)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.t                                                                             <  1:3492796/101‑1 (MQ=255)


                        gtgtCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGAAAG.C.GCCC.CCCCCGGgcgggggggggggc                                                                              >  1:1684912/1‑97 (MQ=18)


                         tgtCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGGGGgggccg                                                                             >  2:3759577/1‑99 (MQ=18)


                          gtCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAA.C.GCCC.GCCCTGGGCGGGGCGGGGGC.Cgg                                                                           >  1:4556132/1‑101 (MQ=21)


                           tCGCGACAGCGTTGCAGAGCCCGCACCCCC.AA.GCGC.GCGAGGGGCGAGCGCCCAGCCCCGGGGGCGCGAAAGCC.C.CC.GCCATGGGCGGGGCGGGCGC.TGGc                                                                          <  1:1626885/101‑1 (MQ=18)


                              cgACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCCGCCCCGGCGGCGCGAAAG.C.GCCC.CCCCTGGGCGGGGCGGGCGC.Cgggccg                                                                       >  2:446670/1‑97 (MQ=17)


                              cgACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCCGCCCCCGCGGCGCGAAAA.CGG.CC.CCCCCGGGCGGGGGGGGGGC.Ggggccg                                                                       >  2:1574412/1‑97 (MQ=16)


                               gcaaGCGTTGAAAACACC.CAACCCC.AACCCGC.GCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.g                                                                     <  1:1193697/98‑1 (MQ=14)


                               gACAGCGTTGCAAAGACCGCATCCCC.AG.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.g                                                                     <  1:1476852/101‑1 (MQ=255)


                               gACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGGAAG.C.GCCC.GCCCCGGGCGGGGGGGGGGC.GGGGCGA.g                                                                     >  2:3784148/1‑101 (MQ=18)


                                aCAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.gg                                                                    <  2:605552/101‑1 (MQ=255)


                                  aGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGAAAA.C.GCCC.GCCATGCGCGGGGGGGGGGC.CGGCCGA.GGG.c                                                                 >  2:2210154/1‑101 (MQ=18)


                                   gggTTGCAAAGCCCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.c.c                                                               <  2:2521135/99‑1 (MQ=255)


                                   gCGTTGCAAAGCCCGCACCCCC.AG.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.c.c                                                               <  2:4167710/101‑1 (MQ=17)


                                   gCGGTGCCAAGACCGTTTCCCC.AG.GCGCAGC.AGAGGCCGGCGCCC.GCCCCGGCGGGCGGGAAG.C.GCCC.CCCATGGGCGGGGCGGGGGC.Cggggcgggggcc                                                                 >  1:682489/1‑91 (MQ=18)


                                    ggTTGAAAAGCCCGCACCCCC.AG.GCGG.GCGAGAGGCCAGCGCCCAGCCCCGGCGGGGCGAAAGCC.C.CC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.t                                                             <  1:3369789/100‑1 (MQ=17)


                                    cGTTGCAAAGCCCGCCCCCCC.AG.GCGC.GCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.t                                                             <  1:3276881/101‑1 (MQ=17)


                                     gtgacAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCGGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.tt                                                            <  2:4320605/97‑1 (MQ=17)


                                     gTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.tt                                                            <  1:2175508/101‑1 (MQ=255)


                                     gTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGGGGGGGC.CGGGCGA.ggggcct                                                               >  1:3219948/1‑97 (MQ=17)


                                     gTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCCGCGGCGCGGAAG.C.GCCC.GCCACGGGCGGGGGGGGGGC.CGGGCGC.ggggcct                                                               >  2:3749466/1‑97 (MQ=17)


                                      ttGCAAAGCCCGCACCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.ttt                                                           <  2:3663242/101‑1 (MQ=17)


                                      tggCAAAGCCCCCCCCCCC.AG.GCGC.GCGAGAGGCCGGCCCCCGGCCCGGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.ttt                                                           <  1:156084/99‑1 (MQ=14)


                                       tGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGGCGG.GGG.C.C.cttt                                                          >  1:2395902/1‑100 (MQ=17)


                                         cAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGGGGGCGC.CGGGCGC.GGG.C.C.CTTtgg                                                        >  1:3568446/1‑101 (MQ=17)


                                           aaGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGCCGC.GGG.G.C.GTTGGGGc                                                      >  2:3977155/1‑101 (MQ=17)


                                            aggccgccccccc.AG.GCGC.GCGAGAGGCGGGCCCCCAGCCCCGGCGGCGCGAAGG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGcc                                                     <  1:3500371/92‑1 (MQ=14)


                                            agcccccaccccc.AA.GCGC.GCGAGGGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGcc                                                     <  1:1045561/92‑1 (MQ=17)


                                               cgccaccccc.AG.GCGC.GCGAGAGCCCGGGCCCCAGCCCCGGCGGCGCAAAAC.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGccccc                                                  <  1:4446280/95‑1 (MQ=14)


                                               ccGCACCCCC.AG.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGccccc                                                  <  2:1863249/101‑1 (MQ=17)


                                                cGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGGGGGGCGGGGGC.CGGGCGG.GGG.C.CTTTTGGGGccccc                                                  >  2:1809987/1‑101 (MQ=17)


                                                cGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCACCCCCGGCGGCGCGAAAG.C.GCCC.GCCACGGGGCGGGGGGGGGC.TGGCCGG.GGG.G.C.CTTGGGGGCCCcg                                                 >  1:392586/1‑101 (MQ=14)


                                                 ccATCCCCAAA.GCGG.GGGAGGGGCTGCCTCCCGGCCCCGCCGGCCAAAAAG.C.G.CCGGCCATGGGCGGGTCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCcg                                                 <  2:1122146/100‑1 (MQ=2)


                                                  cATCCCC.AA.GCGC.GCGAGAGGCCAACGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGCCGA.GGG.G.C.CTTGGGGGCCCcggc                                               >  2:4279655/1‑101 (MQ=17)


                                                   aTCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAA.C.GCCC.GCCCCGGGCGGGGCGGGGGC.CGGCCCA.GGGCC.C.TTTGGGGCCCCcgcg                                               >  2:3493400/1‑99 (MQ=17)


                                                      ccc.AG.GCGC.GCGAGGGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTc                                           <  2:3568178/101‑1 (MQ=17)


                                                      ccc.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGGCGA.GGG.G.C.CTTGGGGGCCCccgcgggc                                           >  1:4155165/1‑99 (MQ=14)


                                                       cc.AG.GCGC.GCGAGAGGCCAGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCa                                          <  2:2761857/101‑1 (MQ=17)


                                                        c.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAA.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGCCGAGGGG.C.C.TTTGGGGCCCCcgcgggcag                                          >  2:3513531/1‑94 (MQ=17)


                                                          aa.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.CGGGCGA.GGG.C.C.TTTGGGGGCCCCGGCGGGCAgg                                        >  1:2224987/1‑101 (MQ=17)


                                                          aa.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAA.C.GCCC.GCCATGGGCGGGGCGGGGGC.CGGGCCA.GGG.G.C.CTTTGGGCCCCCCGCGGGCAgg                                        >  2:4589266/1‑101 (MQ=14)


                                                          aa.GCGC.GCGAGAGGCCAGCGCCCAGCCCCCGCGGCGCCCGAG.C.GCCC.GCCCCGGGCGGGGGGGGGGC.CCGCCGG.GGG.CGC.TTCTGGGCCCCcgccggccgg                                         >  2:3256149/1‑97 (MQ=9)


                                                          aa.GCGC.GCGAGAGGCCAAAGCCCAGCCCCCGCGGGGCGAAAG.C.GCCC.GCCCTGGGCGGGGCGGGGGC.TGGCCCC.GGG.C.C.CCTGTGGCCCCCGGCGGGCAgg                                        >  2:741405/1‑101 (MQ=255)


                                                           a.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.CTTTGGGCCCCCGGCGGGCAggg                                       >  2:2786067/1‑101 (MQ=17)


                                                                c.gcgAGGGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGAt                                   <  2:1419260/101‑1 (MQ=255)


                                                                  gcgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGGGC.TGGCCGG.GGG.C.C.CTTGGGGCCCCCCGCGGGCCgggggggc                                  >  1:4278525/1‑98 (MQ=14)


                                                                   cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCCTGGGCGGGGCGGGCGC.TGGGCGG.GGG.C.C.CTTGGGGCCCCCGGCGGGCCGGGGGGTCt                                 >  2:2969773/1‑101 (MQ=14)


                                                                   cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCCTGGGCGGGGCGGGCGC.CGGCCGA.GGG.C.C.CTTTGGGCCCCCCGCGGGCAggggggcct                                 >  2:3223774/1‑97 (MQ=14)


                                                                   cgAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGGGCTTGGCCGA.GGC.C.C.TTGGGGGCCCCCGCCGGCCAggggggcc                                  >  2:1248585/1‑98 (MQ=14)


                                                                    gaggGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  2:685617/101‑1 (MQ=255)


                                                                    gagaGGCCAGCGGCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGGCAgggggggctg                                >  1:3684064/1‑96 (MQ=14)


                                                                    gagaGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.CTTGGGGCCCCCGGCGGGCAGGGGGGTCTg                                >  2:180082/1‑101 (MQ=14)


                                                                    gagaGGCCAGCCCCCAGCCCGGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTg                                <  2:2459391/101‑1 (MQ=17)


                                                                      ggggCCGGCGCCCGGCCCGGGGGGGGCGAAGGCC.C.CC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  1:124324/99‑1 (MQ=14)


                                                                      gagGCCAGCGCCCGGCCCCGGCGGCGCGAAGG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              <  2:471077/101‑1 (MQ=17)


                                                                      gagGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGGGC.TGGCCGA.GGG.G.C.CTTGGGGCCCCCGGCCGGCAGGGGGGTCTGtt                              >  1:1081399/1‑101 (MQ=14)


                                                                      gagGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGtt                              >  2:1374488/1‑101 (MQ=255)


                                                                       gggCCGGCCCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                             <  2:4463654/100‑1 (MQ=17)


                                                                       agGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTg                             >  2:503376/1‑101 (MQ=255)


                                                                       agGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGCCAGGGGGGTCTGTTg                             >  2:4223005/1‑101 (MQ=17)


                                                                            ggcgcCCACCCCCGGCGGCGCGAAAGCC.C.CC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  1:2070100/100‑1 (MQ=18)


                                                                            ggcccccAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  2:396909/97‑1 (MQ=255)


                                                                            cgcccccGGCCCCGGGGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        <  1:1774033/97‑1 (MQ=18)


                                                                            aGCGCCCAGCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCAt                        >  2:1076932/1‑101 (MQ=255)


                                                                                  cggCCCGGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.T.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgc                  <  1:3551800/99‑1 (MQ=18)


                                                                                   cgCCCCGGCGGCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCcgcg                 <  2:2821686/100‑1 (MQ=255)


                                                                                      ccgggcggcGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCa              <  1:1074386/98‑1 (MQ=255)


                                                                                        cggcggCGCGAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAgc            <  1:1374583/101‑1 (MQ=255)


                                                                                               gcgAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCgg     >  1:97265/1‑101 (MQ=255)


                                                                                               gcgAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCCGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCgg     >  2:301568/1‑101 (MQ=255)


                                                                                                cgAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc    >  1:2200037/1‑101 (MQ=255)


                                                                                                cgAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGc    >  1:1655608/1‑101 (MQ=255)


                                                                                                 gAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGGGCCGCGGcc   >  2:1647160/1‑101 (MQ=255)


                                                                                                 gAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc   <  1:1343818/101‑1 (MQ=255)


                                                                                                 gAAAG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGcc   >  1:3582130/1‑101 (MQ=255)


                                                                                                  aaaG.C.GCCC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCa  <  1:2927684/101‑1 (MQ=255)


GAAAAGCACTGCTTTTCCCCGCCCGTGTCGCGACAGCGTTGCAAAGACCGCATCCCC.AA.GCGC.GCGAGAGGCCAGCGCCCAGCCCCGGCGGCGCGAAAG.C.G.CC.GCCATGGGCGGGGCGGGCGC.TGGCCGA.GGG.C.C.TTTGGGGCCCCCGGCGGTCAGGGGGATCTGTTGCGCATGGCCGCGTCAGCGCCGCGGCCA  >  NC_014034/1867733‑1867927

Base quality scores:


ATCG

< 3 ≤


ATCG

< 12 ≤


ATCG

< 20 ≤


ATCG

< 32 ≤


ATCG

< 41 ≤


ATCG