Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,213 G→A 100% P55L (CCC→CTC)  rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2130G→A100.0% 853.1 222P55L (CCC→CTC) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (112/110):  ref base (0/0):  total (112/110)

Only 100 of 227 total aligned reads displayed.

GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305
                                                                                                  |                                                                                                 
gCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGc                                                                                                 >  1:3918043/1‑101 (MQ=255)
 cGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCa                                                                                                <  1:240756/101‑1 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               >  1:3191885/1‑101 (MQ=255)
  gTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAc                                                                                               <  2:1798735/101‑1 (MQ=255)
   tGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCAcc                                                                                              >  2:3505394/1‑101 (MQ=255)
     aTCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAg                                                                                            >  1:966991/1‑101 (MQ=255)
     aTCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAg                                                                                            <  2:2439590/101‑1 (MQ=255)
         aaaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGcccc                                                                                        >  2:1620413/1‑101 (MQ=255)
          aaTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCa                                                                                       >  1:1428022/1‑101 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  2:876424/101‑1 (MQ=255)
           aTCCCCTTTGCCCCGCCCGCGATGCAGCTTGCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAt                                                                                      <  1:3419792/101‑1 (MQ=255)
             ccccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtct                                                                                    <  1:3356604/101‑1 (MQ=255)
              cccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCAtctc                                                                                   <  2:534802/101‑1 (MQ=255)
               ccTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCg                                                                                  <  1:1869601/101‑1 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 <  1:1654291/101‑1 (MQ=255)
                cTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCgg                                                                                 >  2:716123/1‑101 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                <  2:2351527/101‑1 (MQ=255)
                 tttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGt                                                                                >  1:2252445/1‑101 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               <  2:3700024/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               <  1:4541176/101‑1 (MQ=255)
                  ttGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTc                                                                               >  1:1512668/1‑101 (MQ=255)
                    gCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGc                                                                             >  1:3498978/1‑101 (MQ=255)
                    gCCCCCCCCCGGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGc                                                                             <  2:3800593/101‑1 (MQ=255)
                      cccgcccgCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCt                                                                           <  2:4261745/101‑1 (MQ=255)
                       ccgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCtt                                                                          >  1:257287/1‑101 (MQ=255)
                       atgttcgcgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCtt                                                                          >  2:1870040/6‑101 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  2:1859930/101‑1 (MQ=255)
                        cgcccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCttt                                                                         <  2:1167195/101‑1 (MQ=255)
                          cccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGc                                                                       >  1:894400/1‑101 (MQ=255)
                           ccgcGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGcc                                                                      >  2:2859596/1‑101 (MQ=255)
                              cgATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGaa                                                                   <  1:4123027/101‑1 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  1:2650157/1‑101 (MQ=255)
                               gATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAg                                                                  >  1:2927449/1‑101 (MQ=255)
                                aTGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGc                                                                 <  1:1390674/101‑1 (MQ=255)
                                 tGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCa                                                                >  1:1949905/1‑101 (MQ=255)
                                  gCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAc                                                               >  1:2039475/1‑101 (MQ=255)
                                  gCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAc                                                               <  1:4038214/101‑1 (MQ=255)
                                   cAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAcc                                                              <  1:2805719/101‑1 (MQ=255)
                                      cTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttc                                                           <  2:3060933/101‑1 (MQ=255)
                                        ttCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcct                                                         <  1:4657967/101‑1 (MQ=255)
                                        ttCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcct                                                         <  1:3096612/101‑1 (MQ=255)
                                         tCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcctt                                                        <  2:4492205/101‑1 (MQ=255)
                                         tCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcctt                                                        <  2:4639746/101‑1 (MQ=255)
                                         tCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCAccttcctt                                                        <  1:4273334/101‑1 (MQ=255)
                                           gACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCa                                                      <  2:1225086/101‑1 (MQ=255)
                                           gACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCa                                                      >  2:2952069/1‑101 (MQ=255)
                                              cgccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCt                                                   <  1:217508/101‑1 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  >  1:4416169/1‑101 (MQ=255)
                                               gccgcGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCtt                                                  <  2:2112704/101‑1 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAc                                               <  1:2464851/101‑1 (MQ=255)
                                                  gcgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGAc                                               <  1:851048/101‑1 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  2:2643470/1‑101 (MQ=255)
                                                   cgATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACg                                              >  1:4380373/1‑101 (MQ=255)
                                                    gATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGa                                             >  1:3147951/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            >  1:407151/1‑101 (MQ=255)
                                                     aTCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaa                                            >  2:4601185/1‑101 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           <  1:2535834/101‑1 (MQ=255)
                                                      tCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaa                                           <  2:4521787/101‑1 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          >  2:2042742/1‑101 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          <  1:4294250/101‑1 (MQ=255)
                                                       cTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaa                                          >  2:1529836/1‑101 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  1:3674848/101‑2 (MQ=255)
                                                        tGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGaaaaa                                         <  2:2749652/101‑2 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    <  1:1012316/101‑2 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  1:2434901/1‑100 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    >  1:2276774/1‑100 (MQ=255)
                                                           gTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggg                                    <  2:3714964/101‑2 (MQ=255)
                                                             ttCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggt                                  >  2:4285126/1‑98 (MQ=255)
                                                               ccACATCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttg                                <  1:2109150/101‑6 (MQ=255)
                                                                cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                               >  2:3494976/1‑95 (MQ=255)
                                                                cacaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..aggggttgg                               <  2:91102/101‑7 (MQ=255)
                                                                 acaTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gt                              <  1:1580219/101‑1 (MQ=255)
                                                                  caTCGCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGACCCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtg                             >  1:4005260/1‑100 (MQ=255)
                                                                      gCCATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcgg                         <  1:687946/101‑6 (MQ=255)
                                                                      gCCATCCATCTTGCCCGGATAGGTCAAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcgg                         <  2:2272324/101‑6 (MQ=255)
                                                                       ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                        >  1:535137/1‑95 (MQ=255)
                                                                       ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                        <  1:549359/101‑7 (MQ=255)
                                                                       ccATCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggt                        <  2:3191642/101‑7 (MQ=255)
                                                                         aTCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCGCGGTCGCCTTTGCCGAAGCACCTTCCTTCCTCTTGACGAAAAAGGGGTTg..gtgtcggtct                      <  1:4148473/101‑9 (MQ=255)
                                                                          tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                     >  2:3475521/1‑92 (MQ=255)
                                                                          tCGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTg..gtgtcggtctt                     <  1:175976/101‑10 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  1:331165/1‑101 (MQ=255)
                                                                           cGATCTTGCCCGCATAGGTCGAGAGAACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.g                     >  2:2166392/1‑101 (MQ=255)
                                                                            gATCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.gg                    >  1:3051575/1‑100 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   >  2:840503/1‑99 (MQ=255)
                                                                             aTCTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggt                   <  2:1369670/101‑3 (MQ=255)
                                                                               cTTGCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtga                 <  2:4205787/101‑5 (MQ=255)
                                                                                  gCCCGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatca              <  1:1125871/101‑8 (MQ=255)
                                                                                   cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcag             >  1:4452695/1‑93 (MQ=255)
                                                                                   cccGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcag             <  2:639702/101‑9 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           >  1:107639/1‑91 (MQ=255)
                                                                                     cGCATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTt.ggtgatcagac           >  1:3210523/1‑91 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         >  1:3565271/1‑101 (MQ=255)
                                                                                        aTAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAgg         >  2:1940203/1‑101 (MQ=255)
                                                                                         tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGc        <  1:1134447/101‑1 (MQ=255)
                                                                                          aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcc       <  1:3621927/101‑1 (MQ=255)
                                                                                            gTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGcccc     <  2:3917758/101‑1 (MQ=255)
                                                                                             gggAGAGCACCCGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCg    <  2:1397285/99‑1 (MQ=255)
                                                                                               gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt  >  2:1288617/1‑101 (MQ=255)
                                                                                               gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAAAGGGGTTGGTGTCGGTCTTGGTGATCAGACAGGCCCCGGt  >  2:893827/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCGTGATCAAAATCCCCTTTGCCCCGCCCGCGATGCAGCTTTCGACCGCCGCGATCTGCGTTTCCACATCGCCATCGATCTTGCCCGCATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACGAAAA..GGGTTG..GTCGGTCTT.GTGTACAGACAGGCCCCGGT  >  NC_014034/2177115‑2177305

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG