Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,603,050	A→C	100%	E160A (GAG→GCG)	RCAP_rcc02427 →	M23 family peptidase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,603,050	0	A→C	100.0%	158.9	43	E160A (GAG→GCG)	RCAP_rcc02427	M23 family peptidase
Reads supporting (aligned to +/- strand):  new base (22/21):  ref base (0/0):  total (22/21)

GGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602955‑2603146                                                                                                |                                                                                                 gggcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGGCGGGGTCGACCGCGCCCCCGCCACCCCCCCGCCCCCCGCGCCGc                                                                                             >  2:2745699/1‑101 (MQ=255)   gcgggGTTCCCGCCGCGGGCAGCGAGCGGATCGACACCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                           <  2:4339460/101‑1 (MQ=255)   gcgggGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGccc                                                                                           <  1:1787124/101‑1 (MQ=255)      gggGTGCCGCCGCGGGGAGGGGGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCcgcg                                                                                        <  1:1185542/101‑1 (MQ=255)         cTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGGTCGGCCGCGCCCCCCGCACCCACCCGCCCCCCCCCACCCCCGCCGcc                                                                                     >  2:1149223/1‑101 (MQ=255)          tGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCCCGCGCCCCCGCCCCGCCCGCCCCCCCCCGCCCCGCGCCGCGCCCCCGGccc                                                                                    >  2:247513/1‑101 (MQ=255)          ggccgccGCGGGCGGCGAGCGGATCGAGATCACCTCGCTGGGGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGccc                                                                                    <  2:4244542/100‑1 (MQ=255)           gccgccGCGGGCAGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGcccc                                                                                   <  1:1444079/101‑1 (MQ=255)           gcccccGCGGGCAGCGAGCGGATCGACATCGCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGcccc                                                                                   <  2:4628916/101‑1 (MQ=255)               ccgcGGGCGGCGAGCGGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGcc                                                                               <  1:3562493/101‑1 (MQ=255)                 gggggCGGCGAGCGGATCGACATCCCCCGGCGGGGGGGGGCCGCGATCGCCCCCGCCCCCGCCCCCCACCCCCCCGCCGCGACGCCCGCGGCCCCCGCCAc                                                                             <  2:1226480/99‑1 (MQ=255)                    ggCGGCGAGCGGATCGACATCACCCCGCTGGGGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACcgc                                                                          <  1:1212555/101‑1 (MQ=255)                    ggCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGGCCCGGGTGTCCGCGCCCCCGCCCCCACGCCCCCCCCCGCGCCGCCCCCGCCCCCCCCCCcccc                                                                          >  1:3937005/1‑99 (MQ=255)                     gCAGCGAGCGGAGCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGCCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCAAcgcg                                                                         <  2:947697/101‑1 (MQ=255)                        gcgagcgGATCGACATCCCCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGccc                                                                      <  1:83262/101‑1 (MQ=255)                            gcgcctCGCCATCACCGCGCTGCCGGGGGCCGCGATCGACCGCGCCGCGTTCCCCCAGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTc                                                                  <  1:3953035/96‑1 (MQ=255)                             cgGATCGACATCACCTCGCTGGCGGGGGGCGCGAGCGACCGCGCCGCCCGCACCCCGCCGCCCCCCGCGGCGCCCCCGGCCCCCCCCCCCCCGcccccccc                                                                 >  2:2683397/1‑97 (MQ=255)                                   gACATCACCTCGCTGGCGGGGGGCGCGGTCGGCCGCGCCCCCGGCACCCACCCGCCCCCCCCCCCCCCCCCGGCCCCCCCCCCCGCCGCCCCCCCccccgc                                                           >  2:1267205/1‑101 (MQ=255)                                   gACATCACCTCGCTGGCGGGGGCCCCGGGCGGCCGCGCCGCCGGCACCCCCCCGCCCGCCCCGACGCCCCCGGCCCCCCCCCACCCGCCCCGCccccgccc                                                           >  2:679569/1‑96 (MQ=255)                                              gcgggCGGGGCCCGCGACCGCCCGCGCCGCCGTCCCCCGCCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCa                                                <  2:3936720/98‑1 (MQ=255)                                                    gggggCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCgg                                          >  2:2961646/1‑101 (MQ=255)                                                     gggcCCGCGCCCGACCGCCCCCCCGTCCCCCACCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGt                                         <  2:898355/101‑1 (MQ=255)                                                            cgATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGaaa                                  >  2:158888/1‑101 (MQ=255)                                                             gATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAAc                                 >  1:3479470/1‑101 (MQ=255)                                                              aTCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAAcc                                >  2:2190530/1‑101 (MQ=255)                                                               tCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCg                               >  1:3087637/1‑101 (MQ=255)                                                               ccgcccGCCCCCCCGCCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCg                               <  2:4290771/97‑1 (MQ=255)                                                                 gACCGCGCCGCCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgc                             >  2:3807001/1‑101 (MQ=255)                                                                 gACCGCGCCGCCGCCCCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgc                             <  2:403187/101‑1 (MQ=255)                                                                   ccGCGCCGCCGCCACCCAGCCGCCCGCCGCGCCGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGgccg                           <  2:4157809/101‑1 (MQ=255)                                                                      cgccgcCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgccc                        >  1:2996594/1‑101 (MQ=255)                                                                      cgccgcCGTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgccc                        >  1:386617/1‑101 (MQ=255)                                                                        cccccgccccccGGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgccccc                      <  1:1149315/92‑1 (MQ=255)                                                                        ccccccccccccACCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgccccc                      <  2:3572744/92‑1 (MQ=255)                                                                         cgccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccg                     >  2:3345405/1‑101 (MQ=255)                                                                         cgccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccg                     >  1:54529/1‑101 (MQ=255)                                                                          gccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgc                    >  1:798278/1‑101 (MQ=255)                                                                           ccgccccccACCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgcc                   <  2:1098816/95‑1 (MQ=255)                                                                           ccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgcc                   >  1:2850861/1‑101 (MQ=255)                                                                           ccgTCACCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGccgcccccgcc                   >  1:112997/1‑101 (MQ=255)                                                                             gccccccAGCCCCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCa                 <  2:3479212/97‑1 (MQ=255)                                                                               cccccgcccccccgccGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGa               <  1:2961875/91‑1 (MQ=255)                                                                                cccaggcccccCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAc              <  1:556084/96‑1 (MQ=255)                                                                                aCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAc              >  2:302106/1‑101 (MQ=255)                                                                                aCCCAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAc              >  2:1049208/1‑101 (MQ=255)                                                                                 cccAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCTAAACCGGCCGCCCCCGCCCAGAcc             >  1:4598390/1‑101 (MQ=255)                                                                                 cccAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGAcc             >  1:3852248/1‑101 (MQ=255)                                                                                   cAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCgg           >  2:1319383/1‑101 (MQ=255)                                                                                   cAGCCGCCCGCCGCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCgg           >  1:298397/1‑101 (MQ=255)                                                                                           cgccgCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagc   >  2:1201150/1‑101 (MQ=255)                                                                                           cgccgCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagc   >  1:3309207/1‑101 (MQ=255)                                                                                            gccgCGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCgccgagcc  >  2:39125/1‑101 (MQ=255)                                                                                                |                                                                                                 GGGCGGGGCTGCCGCCGCGGGCAGCGAGCGGATCGACATCACCTCGCTGGCGGGGGCCGCGATCGACCGCGCCGCCGTCACCCAGCCGCCCGCCGAGACGCCCGCGGCCCCCGCCACCGCGCCCCGTCCCGCCGCGCCAAGTGCCAGCCCGGTGGCGAAACCGGCCGCCCCCGCCCAGACCGGCGCCGAGCC  >  NC_014034/2602955‑2603146 Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG