Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,630,045 T→C 100% E120E (GAA→GAG potD4 ← polyamine ABC transporter periplasmic polyamine‑binding protein PotD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,630,0450T→C100.0% 852.8 223E120E (GAA→GAGpotD4polyamine ABC transporter periplasmic polyamine‑binding protein PotD
Reads supporting (aligned to +/- strand):  new base (91/131):  ref base (0/0):  total (91/132)

Only 100 of 230 total aligned reads displayed.

AACAGCAGGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCTTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGCCCGCCAGCC  >  NC_014034/2629947‑2630142
                                                                                                  |                                                                                                 
aaCAGCAGGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTc                                                                                                 <  1:93080/101‑1 (MQ=255)
aaCAGCAGGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCACCCTc                                                                                                 <  2:3704079/101‑1 (MQ=255)
 aCAGCAGGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCg                                                                                                >  1:3543043/1‑101 (MQ=255)
  cagcagGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGATCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGa                                                                                               >  1:3242825/1‑101 (MQ=255)
  cagcagGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGa                                                                                               <  2:3014077/101‑1 (MQ=255)
    gcagGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGAcc                                                                                             <  2:2579833/101‑1 (MQ=255)
    gcagGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGAcc                                                                                             <  1:65203/101‑1 (MQ=255)
      agGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCAc                                                                                           <  1:2568069/101‑1 (MQ=255)
      agGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCAc                                                                                           <  1:1536783/101‑1 (MQ=255)
       ggCCGATGGAATCGGGCGTGCCGGTGACGTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACg                                                                                          <  1:111249/101‑1 (MQ=255)
        gCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCGGGTTTCGCCCTCGACCACGa                                                                                         >  1:487272/1‑101 (MQ=255)
        gCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCAACGAGACCGAACCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGa                                                                                         >  2:4667790/1‑101 (MQ=255)
         ccGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAt                                                                                        <  2:4440687/101‑1 (MQ=255)
         ccGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAt                                                                                        <  2:1472928/101‑1 (MQ=255)
         ccGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAt                                                                                        >  1:2754771/1‑101 (MQ=255)
         ccGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAt                                                                                        >  1:2210597/1‑101 (MQ=255)
           gATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGCtct                                                                                      >  1:820058/1‑101 (MQ=255)
           gATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtct                                                                                      >  2:2197670/1‑101 (MQ=255)
           gATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtct                                                                                      <  1:2507460/101‑1 (MQ=255)
            aTGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtctc                                                                                     >  1:1502993/1‑101 (MQ=255)
            aTGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtctc                                                                                     <  2:4155545/101‑1 (MQ=255)
            aTGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtctc                                                                                     <  2:3597099/101‑1 (MQ=255)
            aTGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGAtctc                                                                                     <  2:2298568/101‑1 (MQ=255)
             tGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCg                                                                                    <  1:2789975/101‑1 (MQ=255)
                 aTCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATAAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGAt                                                                                >  1:4414376/1‑101 (MQ=255)
                  tCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATg                                                                               <  1:4353263/101‑1 (MQ=255)
                    gggCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGtt                                                                             >  1:2341201/1‑101 (MQ=255)
                    gggCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGtt                                                                             <  1:3559996/101‑1 (MQ=255)
                       cGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTgg                                                                          <  1:4090251/101‑1 (MQ=255)
                        gTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGc                                                                         >  2:4306984/1‑101 (MQ=255)
                        gTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGAGGCACCGCCCAGGGTTCGCCCTCGACCACGATCTCGGGATGTTTgtc                                                                         >  2:2216737/1‑99 (MQ=255)
                            ccggtaCCTTGTCGGGGCGGTACATCACCGAGACCGAGCGCCAGATCAGCGGCTCCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAAc                                                                     <  2:4257667/96‑1 (MQ=255)
                              gTGCCCTTGGCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTc                                                                   <  1:2055501/101‑1 (MQ=255)
                              gTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTc                                                                   <  2:1768201/101‑1 (MQ=255)
                              gTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTc                                                                   <  2:724952/101‑1 (MQ=255)
                               tGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCg                                                                  >  2:1092798/1‑101 (MQ=255)
                                gACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCgg                                                                 <  1:1918770/101‑1 (MQ=255)
                                 aCCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCggg                                                                >  1:1245457/1‑101 (MQ=255)
                                 aCCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCggg                                                                <  2:3419022/101‑1 (MQ=255)
                                   cTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGAt                                                              <  2:196818/101‑1 (MQ=255)
                                   cTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGAt                                                              >  2:4369176/1‑101 (MQ=255)
                                   cTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGAt                                                              <  2:2366678/101‑1 (MQ=255)
                                    ttGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATa                                                             <  2:1508950/101‑1 (MQ=255)
                                    ttGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATa                                                             <  2:3948256/101‑1 (MQ=255)
                                        cGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATg                                                         <  2:2286556/101‑1 (MQ=255)
                                         ggggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGc                                                        <  1:3693999/101‑1 (MQ=255)
                                         ggggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGc                                                        <  1:1380015/101‑1 (MQ=255)
                                         ggggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGc                                                        <  2:4513958/101‑1 (MQ=255)
                                         ggggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCCCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGc                                                        <  1:316365/101‑1 (MQ=255)
                                          gggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGcc                                                       >  2:4395925/1‑101 (MQ=255)
                                           ggCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGccc                                                      >  2:3531129/1‑101 (MQ=255)
                                            gCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGcccc                                                     >  2:2787172/1‑100 (MQ=255)
                                             cGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTc                                                    <  1:2646146/101‑1 (MQ=255)
                                             cGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTc                                                    <  1:2832320/101‑1 (MQ=255)
                                             cGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTc                                                    <  2:811758/101‑1 (MQ=255)
                                              ggTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCg                                                   <  2:4165615/101‑1 (MQ=255)
                                              ggTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCg                                                   <  1:4281913/101‑1 (MQ=255)
                                              ggTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCg                                                   <  1:4140577/101‑1 (MQ=255)
                                                tAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGc                                                 <  2:1300189/101‑1 (MQ=255)
                                                 aGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGGTGTTTGGCGAACTCGGGATAGATGCCCTCGGCt                                                >  1:1667987/1‑101 (MQ=255)
                                                 aGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCt                                                <  1:1996636/101‑1 (MQ=255)
                                                  gATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTc                                               <  1:4244432/101‑1 (MQ=255)
                                                    tCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCg                                             <  1:2290663/101‑1 (MQ=255)
                                                      accgagaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCggc                                           >  2:4574614/1‑100 (MQ=255)
                                                      accgagaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGt                                           <  2:4060104/101‑1 (MQ=255)
                                                       ccgagaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTc                                          >  2:1710864/1‑101 (MQ=255)
                                                        cgagaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCa                                         <  2:3242580/101‑1 (MQ=255)
                                                         gagaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCAc                                        <  1:3285806/101‑1 (MQ=255)
                                                          agaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCAcc                                       <  2:217343/101‑1 (MQ=255)
                                                           gaccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCg                                      >  2:2032524/1‑101 (MQ=255)
                                                            accgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGt                                     >  1:1467013/1‑101 (MQ=255)
                                                             ccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTg                                    >  1:1619615/1‑101 (MQ=255)
                                                             ccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTg                                    <  2:1914006/101‑1 (MQ=255)
                                                             ccgagCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTg                                    >  1:376784/1‑101 (MQ=255)
                                                                    ccAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCggggg                             >  2:1952877/1‑100 (MQ=255)
                                                                    ccAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGAGAGATGCCCTCGGCTCCGGTCACCGTGGCggggg                             >  1:3968343/1‑100 (MQ=255)
                                                                     cAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCgggggc                            >  1:1143935/1‑99 (MQ=255)
                                                                     cAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTc                            <  1:4396217/101‑1 (MQ=255)
                                                                     cAGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTc                            <  1:3360616/101‑1 (MQ=255)
                                                                      aGATCAGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCggggggg                           >  1:59863/1‑98 (MQ=255)
                                                                           aGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCgg                      <  1:958812/101‑1 (MQ=255)
                                                                           aGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCgg                      <  1:1654959/101‑1 (MQ=255)
                                                                           aGCGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCgg                      <  2:3799215/101‑1 (MQ=255)
                                                                             cGGCACCTCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGAt                    <  1:3018749/101‑1 (MQ=255)
                                                                             cGGCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGAt                    <  2:2542296/101‑1 (MQ=255)
                                                                               gCACCGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGGCGACCGGGTcg                  >  2:1102896/1‑101 (MQ=255)
                                                                                cACCGCCCAGGTTTCGCCGTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATcga                 <  2:3093825/101‑1 (MQ=255)
                                                                                 cccGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATcgac                <  2:140096/100‑1 (MQ=255)
                                                                                   cGCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTGGACCGGGTcgacga              >  2:289763/1‑101 (MQ=255)
                                                                                    gCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGaa             <  1:3664124/101‑1 (MQ=255)
                                                                                    gCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGaa             <  1:4034123/101‑1 (MQ=255)
                                                                                    gCCCAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGAACGGGTCGGCGaa             >  2:1192593/1‑101 (MQ=255)
                                                                                     cccAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAg            <  2:62175/101‑1 (MQ=255)
                                                                                     cccAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGGCGACCGGATCGACGAAg            >  2:4098025/1‑101 (MQ=255)
                                                                                     cccAGGTTTCGCCCTCCACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGGCGACCGGAttgtggaag            >  1:2191939/1‑93 (MQ=255)
                                                                                      ccAGGTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGc           <  1:1835743/101‑1 (MQ=255)
                                                                                         ggTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGcccc        >  2:2435801/1‑100 (MQ=255)
                                                                                          gTTTCGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGCCCGc       <  2:2638691/101‑1 (MQ=255)
                                                                                              cGCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGGCGACCGGATCGACGAAGCCCGCCCGc   >  1:2974942/1‑101 (MQ=255)
                                                                                               gCCCTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGCCCGCCAGcc  <  2:1638499/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
AACAGCAGGCCGATGGAATCGGGCGTGCCGGTGACCTTGTCGGGGCGGTAGATCACCGAGACCGAGCCCCAGATCAGCGGCACCGCCCAGGTTTCGCCTTCGACCACGATCTCGGGATGTTTGGCGAACTCGGGATAGATGCCCTCGGCTCCGGTCACCGTGGCGGGGTCGACCGGATCGACGAAGCCCGCCAGCC  >  NC_014034/2629947‑2630142

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG