Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,192 A→G 21.8% intergenic (‑168/+108) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,1920A→G21.8% 78.1 0.0 0.0150intergenic (‑168/+108)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (15/18):  ref base (54/63):  total (69/81)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 156 total aligned reads displayed.

CGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640095‑2640291
                                                                                                     |                                                                                                     
cggcggCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGagc                                                                                                     <  1:1112830/101‑3 (MQ=255)
    ggcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGa                                                                                                 <  1:345314/101‑1 (MQ=37)
     gccagCGTGTGAACCGTGGCGCTTTTGCTCAGGCGAAAGGGGGGCTGTGCCCCCCT.CTT.G.GCCTTCGGGCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                <  2:513021/97‑1 (MQ=21)
     gcAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGAt                                                                                                <  1:1820549/101‑1 (MQ=37)
      cAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATg                                                                                               <  2:1805999/101‑1 (MQ=37)
       aGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGa                                                                                              <  2:2367902/101‑1 (MQ=37)
        ggCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                             <  1:2184471/101‑1 (MQ=25)
         gCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaa                                                                                            <  1:1897055/101‑1 (MQ=25)
          cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGGGCTGCCCCCCT.CTTCT.C.CTTCGGCCAATTCACCCCCCCCGGTATTTTGAGCAAGATGaaaa                                                                                           >  2:1682965/1‑101 (MQ=25)
          cGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                           <  1:58641/101‑2 (MQ=25)
               catccGTGGCGCTTTTGCGCACGCCATATCGGGGCGCAGCGCCCCT.CTT.GCA.CTTAGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATGaaatgccat                                                                                      <  2:2492458/98‑7 (MQ=255)
                     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                             <  2:553602/101‑1 (MQ=21)
                     tGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                             <  1:2114179/101‑1 (MQ=21)
                     gggCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTc                                                                             <  2:2139839/100‑1 (MQ=25)
                       gcgcTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCAt                                                                           <  2:898430/101‑1 (MQ=25)
                          cTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCtt                                                                        <  1:3304586/101‑1 (MQ=21)
                              tGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGcgt                                                                    <  2:65143/101‑3 (MQ=21)
                               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTTAG.C.CTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                   <  1:4563587/101‑1 (MQ=25)
                               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                   <  2:2584354/101‑1 (MQ=25)
                               gcgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTc                                                                   <  1:2311517/101‑1 (MQ=21)
                                cgcAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCa                                                                  <  2:3073057/101‑1 (MQ=25)
                                   agccAAAAGGGGGCCGCTGCCCCCCT.CTT.G.GTCTTCGGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAat                                                               <  2:746158/98‑1 (MQ=25)
                                    ggCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAata                                                              <  2:3575984/101‑1 (MQ=21)
                                       gaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATccc                                                          <  1:1215644/100‑1 (MQ=17)
                                       gaaaAGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTANCTTGCCCCAAATATccc                                                          >  2:1778669/2‑101 (MQ=17)
                                        aaaGGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATccc                                                          <  2:3189647/101‑1 (MQ=21)
                                        aaaGGGGGGC.CCGCCCCCCTCCTT.G.G.CCTCCGGCAAATCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCg                                                         <  1:3006725/101‑1 (MQ=21)
                                        aaaAGGGGGCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCg                                                         <  2:2748614/101‑1 (MQ=17)
                                         aaagggggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgg                                                        >  2:3705510/4‑101 (MQ=17)
                                           agggggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggg                                                      >  2:1578326/2‑101 (MQ=18)
                                            gggggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggg                                                      <  2:1352989/101‑1 (MQ=25)
                                            gggggCGCTG.CCCCCT.CTT.G.GCCTTTGGCCAATTC.ACCCCTGGGATATTTCGGGCAAGATGAA...ATCATGCCCCTTCATCTTGCCGCAAATATCCCgggggg                                                    <  2:2120767/101‑1 (MQ=2)
                                              gggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtg                                                   >  2:3699403/1‑99 (MQ=18)
                                              gggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCgggggg                                                    >  2:2721403/1‑101 (MQ=25)
                                               ggCGCTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                  <  1:4092162/101‑4 (MQ=21)
                                               gccgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtga                                                  <  1:1084325/99‑4 (MQ=21)
                                                gcgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  1:3417906/101‑5 (MQ=21)
                                                gcgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  2:4149903/101‑5 (MQ=21)
                                                gcgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgag                                                 <  1:4293630/101‑5 (MQ=21)
                                                 cgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCGCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                <  2:3939473/101‑6 (MQ=21)
                                                 cgcTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgagg                                                <  2:240521/101‑6 (MQ=21)
                                                   ttGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcc                                               <  1:2032567/100‑1 (MQ=25)
                                                   cTGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggtgaggcg                                              >  1:2889601/1‑94 (MQ=25)
                                                    tGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTcca                                              >  2:2032427/1‑100 (MQ=25)
                                                    tGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCt                                              <  1:3683008/101‑1 (MQ=25)
                                                    tGCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCCGGAGATTTGCGGCAAGGTGAAAGGA..TGG..GGTATATCTTGCCCCCAATATCCCgagggtgcggcga                                             >  2:317634/1‑93 (MQ=18)
                                                     gCCCCCCT.CTT.G.GCCTTCGGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGG..GGTTTATCTTGCCCCAAATATCCCgggggggaggcgaa                                            >  1:127641/1‑93 (MQ=25)
                                                      ccccccT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                            >  2:1899673/1‑101 (MQ=25)
                                                      ccccccT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGa                                            >  2:1541300/1‑101 (MQ=25)
                                                         cccT.CTT.G.GACTTCGGCCAATTCGCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAg                                         <  1:2032281/101‑1 (MQ=37)
                                                          ccT.CTT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                        >  1:462203/1‑101 (MQ=37)
                                                           ct.ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                       >  1:3527331/1‑101 (MQ=37)
                                                            t.ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  1:1242583/101‑1 (MQ=37)
                                                            t.ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      >  1:3813506/1‑101 (MQ=37)
                                                            t.ctT.G.GCCTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGAc                                      <  1:2882076/101‑1 (MQ=37)
                                                                    gccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggggg                                 <  2:1989697/99‑1 (MQ=37)
                                                                     ccTTCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggggg                                >  1:4620347/2‑101 (MQ=37)
                                                                       ttCGGACAAGTCACCCCCCGAGGTATTTGGGGCAAGATGAAAA.ACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                              <  2:1841463/101‑1 (MQ=25)
                                                                        tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             >  2:1080611/1‑101 (MQ=37)
                                                                        tCGGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                             <  1:4496931/101‑1 (MQ=255)
                                                                          ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           <  1:1063811/101‑1 (MQ=255)
                                                                          ggCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                           >  1:1294296/1‑101 (MQ=37)
                                                                            ccAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcc                         >  1:2359969/1‑101 (MQ=37)
                                                                             cAATTCACCCACCGAGGGATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                        >  2:2318962/1‑101 (MQ=25)
                                                                              aaTTCACCACCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCCTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                       >  2:1652292/1‑101 (MQ=37)
                                                                               aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  1:4191721/1‑101 (MQ=255)
                                                                               aTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                      >  2:2746508/1‑101 (MQ=37)
                                                                                 tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                    >  1:914169/4‑101 (MQ=25)
                                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   >  1:1358911/3‑101 (MQ=255)
                                                                                  caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                   <  2:4109499/99‑1 (MQ=255)
                                                                                   accccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTcc                  >  2:1366748/2‑101 (MQ=255)
                                                                                    ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  1:1749994/101‑1 (MQ=255)
                                                                                    cccaccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                 <  1:2319124/101‑1 (MQ=255)
                                                                                     cccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCg                >  2:3565847/1‑101 (MQ=255)
                                                                                      ccccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCgg               >  2:2959146/1‑101 (MQ=255)
                                                                                        ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt             >  1:1167436/1‑101 (MQ=255)
                                                                                        ccGAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGt             >  2:426744/1‑101 (MQ=255)
                                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:34989/1‑101 (MQ=255)
                                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  2:2066322/1‑101 (MQ=255)
                                                                                          gAGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt           >  1:4412355/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:4329800/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:1691652/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  2:289868/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  2:3117512/1‑101 (MQ=255)
                                                                                           aGGTATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt          >  1:2481085/1‑101 (MQ=255)
                                                                                              tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:3314646/1‑101 (MQ=255)
                                                                                              tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  2:3426415/1‑101 (MQ=255)
                                                                                              tATTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct       >  1:756393/1‑101 (MQ=255)
                                                                                               aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  2:2473816/1‑101 (MQ=255)
                                                                                               aTTTGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGAACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc      >  1:1498210/1‑101 (MQ=255)
                                                                                                tttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  1:3473510/1‑101 (MQ=255)
                                                                                                tttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa     >  2:3242799/1‑101 (MQ=255)
                                                                                                 ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    <  1:2721600/101‑1 (MQ=255)
                                                                                                 ttGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa    >  1:3563661/1‑101 (MQ=255)
                                                                                                  tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   >  1:2135655/1‑101 (MQ=255)
                                                                                                  tGGAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg   <  1:2606822/101‑1 (MQ=255)
                                                                                                   ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:2639042/1‑101 (MQ=255)
                                                                                                   ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  <  2:3813221/101‑1 (MQ=255)
                                                                                                   ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  1:1476741/1‑101 (MQ=255)
                                                                                                   ggAGCAAGATG.AAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc  >  2:1075966/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CGGCGGCAGGCGTGTCAACCGTGGCGCTTTTGCGCAGGCAAAAGGGGGGCGCTGCCCCCCT.CTT.G.G.CTTCGGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATG..GGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGC  >  NC_014034/2640095‑2640291

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG