Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,042,953 G→T 100% W217L (TGG→TTG)  thiD → phosphomethylpyrimidine kinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,042,9530G→T100.0% 816.1 212W217L (TGG→TTG) thiDphosphomethylpyrimidine kinase
Reads supporting (aligned to +/- strand):  new base (115/96):  ref base (0/0):  total (116/96)

Only 100 of 223 total aligned reads displayed.

CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCGG  >  NC_014034/3042855‑3043051
                                                                                                  |                                                                                                  
cGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTtgg                                                                                                  >  2:876474/1‑101 (MQ=255)
cGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTtgg                                                                                                  <  2:579958/101‑1 (MQ=255)
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACTCCCATGGCACCGGCTGCGCCCTGTCTTCGGCCTTGGc                                                                                                 <  1:2241221/101‑1 (MQ=255)
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGc                                                                                                 >  1:1127641/1‑101 (MQ=255)
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGc                                                                                                 >  1:3217690/1‑101 (MQ=255)
 gATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGc                                                                                                 >  1:355795/1‑101 (MQ=255)
    ctgctgCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGAc                                                                                              >  1:4435160/1‑101 (MQ=255)
    ctgctgCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTAGGCCTTGGCGAc                                                                                              >  2:4477604/1‑101 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                            >  2:2608697/1‑101 (MQ=255)
      gctgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGc                                                                                            >  1:541407/1‑101 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                          >  1:2781292/1‑101 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                          <  1:1585692/101‑1 (MQ=255)
        tgctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAg                                                                                          <  2:3010106/101‑1 (MQ=255)
         gctCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGc                                                                                         >  1:3733180/1‑101 (MQ=255)
               tGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGccc                                                                                   <  2:1256314/101‑1 (MQ=255)
               tGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGccc                                                                                   <  2:3698927/101‑1 (MQ=255)
                 ccGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGc                                                                                 >  1:455220/1‑101 (MQ=255)
                  cgcgGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCa                                                                                >  1:2796378/1‑101 (MQ=255)
                      ggCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATccc                                                                            <  1:2632476/101‑1 (MQ=255)
                       gCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcccc                                                                           <  1:4334115/101‑1 (MQ=255)
                       gCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATcccc                                                                           <  2:275440/101‑1 (MQ=255)
                         gAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGa                                                                         >  2:79819/1‑101 (MQ=255)
                            gTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCt                                                                      <  1:1056461/101‑1 (MQ=255)
                             tCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTg                                                                     >  2:921612/1‑101 (MQ=255)
                               tGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTgcg                                                                   <  1:425638/101‑1 (MQ=255)
                                 gTTCGAGGCGCCGCGGGTCGAGACGAAAAACCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGcc                                                                 <  2:3861831/101‑1 (MQ=255)
                                  ttCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCa                                                                <  2:11743/101‑1 (MQ=255)
                                   tCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAg                                                               <  1:76830/101‑1 (MQ=255)
                                    cGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAgg                                                              >  2:1320172/1‑101 (MQ=255)
                                     gAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgc                                                             >  2:4309888/1‑101 (MQ=255)
                                     gAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgc                                                             <  1:911083/101‑1 (MQ=255)
                                       ggCGCCGCGGGTCGAGACGAAAATCACCCGTGGCACCGGGTGCTCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccg                                                           <  2:2985153/101‑1 (MQ=255)
                                       ggCGCCGCGGGTCGAGACGAAAAACCCCCATGGCACCGGCCGCACCCTGTCTTCGGCCTTGTCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccg                                                           <  2:3514606/101‑1 (MQ=255)
                                       ggCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccg                                                           >  2:2173979/1‑101 (MQ=255)
                                          gcggcgAGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccg                                                        <  1:814906/98‑1 (MQ=255)
                                          gccgcGGGTCGAGACGAAAAACGCCCAGGGCACCGGCTGCGCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccg                                                        <  1:2967465/101‑1 (MQ=255)
                                             gcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccg                                                     >  1:3842750/1‑101 (MQ=255)
                                             gcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccg                                                     >  2:2309472/1‑101 (MQ=255)
                                             gcgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccg                                                     >  1:4656301/1‑101 (MQ=255)
                                              cgGGTCGAGACGAAAAACCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgc                                                    <  1:647058/101‑1 (MQ=255)
                                              cgGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgc                                                    >  2:4243022/1‑101 (MQ=255)
                                                ggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccg                                                  <  2:3338030/101‑1 (MQ=255)
                                                ggTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccg                                                  >  2:3187369/1‑101 (MQ=255)
                                                 gTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGgccgccgccgccgc                                                 >  1:3732063/1‑101 (MQ=255)
                                                    gagaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgcccg                                              <  2:3742422/101‑1 (MQ=255)
                                                    gagaCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGccgcccg                                              <  1:107798/101‑1 (MQ=255)
                                                       aCGGAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCgcgc                                           >  1:4523309/1‑101 (MQ=255)
                                                       aCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCgcgc                                           >  2:3726049/1‑101 (MQ=255)
                                                       aCGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCgcgc                                           >  1:3812473/1‑101 (MQ=255)
                                                        cGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGcc                                          >  1:4064580/1‑101 (MQ=255)
                                                        cGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGcc                                          >  1:1252763/1‑101 (MQ=255)
                                                        cGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGcc                                          >  2:4001206/1‑101 (MQ=255)
                                                         gAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                         <  1:312678/101‑1 (MQ=255)
                                                         gAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCt                                         >  2:706483/1‑101 (MQ=255)
                                                          aaaaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCta                                        <  1:202993/101‑1 (MQ=255)
                                                          aaaaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCta                                        >  1:3424467/1‑101 (MQ=255)
                                                          aaaaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGACTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCta                                        <  1:3995286/101‑1 (MQ=255)
                                                           aaaaCCCCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtat                                       <  1:1761548/101‑1 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                      <  2:138817/101‑1 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                      <  2:1618321/101‑1 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                      >  2:3559320/1‑101 (MQ=255)
                                                            aaaCACCCATGGCACCGGCTGCACCCTGCCTTCGGCCTTGGCGACCCAGCTGGCCCGCATCCCCGATCAGCGCCAGGCCGCCGCCGCCGCCCGCGCCtata                                      >  1:2316024/1‑101 (MQ=255)
                                                               cacCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGc                                   >  1:3179042/1‑101 (MQ=255)
                                                                acCCATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCt                                  >  1:2543808/1‑101 (MQ=255)
                                                                 cccATGGCACTGGCTGCTCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCAGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTg                                 <  1:1528037/101‑1 (MQ=255)
                                                                 cccATGGCACCGGCTGCCCCCTGTCTGCGGCCTTGGCGACGCAGCCGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTg                                 <  1:3921460/101‑1 (MQ=255)
                                                                  ccATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgc                                <  2:2579930/101‑1 (MQ=255)
                                                                   cATGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcg                               >  1:2873313/1‑101 (MQ=255)
                                                                     tGGCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgcg                             >  2:1627133/1‑101 (MQ=255)
                                                                      ggCACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTgcgcgc                            <  2:465447/101‑1 (MQ=255)
                                                                        cACCGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCa                          <  1:1367850/101‑1 (MQ=255)
                                                                          ccGGCTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATc                        <  1:2602029/101‑1 (MQ=255)
                                                                            ggCTGCACCCTGTCTCCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgc                      <  2:2489607/101‑1 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                    <  2:2011558/101‑1 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                    <  1:3187612/101‑1 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                    >  1:2325708/1‑101 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                    <  2:4064294/101‑1 (MQ=255)
                                                                              cTGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccg                    >  1:3714630/1‑101 (MQ=255)
                                                                               tGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                   <  2:3046417/101‑1 (MQ=255)
                                                                               tGCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATcgccgc                   <  1:2706510/101‑1 (MQ=255)
                                                                                gCACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCcgcg                  >  1:1698431/1‑101 (MQ=255)
                                                                                 cACCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCgg                 >  2:3344066/1‑101 (MQ=255)
                                                                                  ccccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                <  2:1680564/100‑1 (MQ=255)
                                                                                  ccccTGTCTTCGGCCTGGGCGACGCAGCTGGCCGGCATCCCCGATCTGCGCCAGGCCGCCGCGGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                <  1:2161158/100‑1 (MQ=255)
                                                                                  aaccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                <  1:3040043/99‑1 (MQ=255)
                                                                                  aCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                >  2:2849217/1‑101 (MQ=255)
                                                                                  aCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                >  2:3972255/1‑101 (MQ=255)
                                                                                  aCCCTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGc                >  2:3924756/1‑101 (MQ=255)
                                                                                   cccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGcc               <  2:3145057/101‑1 (MQ=255)
                                                                                   cccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGcc               >  2:911608/1‑101 (MQ=255)
                                                                                    ccTGTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCg              >  2:2144366/1‑101 (MQ=255)
                                                                                      ggTCTTCGGCCTCGGCGACGCAGCTGGCCCGCCTCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCTCCTATACGCTGCGCGCCATCGCCGCGGCCgct            <  2:2936861/100‑3 (MQ=255)
                                                                                       gTCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGAcc           <  1:2139524/101‑1 (MQ=255)
                                                                                        tCTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCg          >  2:2333833/1‑101 (MQ=255)
                                                                                         cgtCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCgg         <  2:355763/99‑1 (MQ=255)
                                                                                         cTTCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCgg         >  2:1016218/1‑101 (MQ=255)
                                                                                          ttCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGc        >  1:237540/1‑101 (MQ=255)
                                                                                          ttCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGc        <  1:3578347/101‑1 (MQ=255)
                                                                                           tCGGCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCt       >  1:2449384/1‑101 (MQ=255)
                                                                                              gCCTTGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTtc    >  1:1498141/1‑101 (MQ=255)
                                                                                                  |                                                                                                  
CGATCTGCTGCTCGATGCCGCGGGCGAGGTCTGGTTCGAGGCGCCGCGGGTCGAGACGAAAAACACCCATGGCACCGGCTGCACCCTGTCTTCGGCCTGGGCGACGCAGCTGGCCCGCATCCCCGATCTGCGCCAGGCCGCCGCCGCCGCCCGCGCCTATACGCTGCGCGCCATCGCCGCGGCCGACCGGCTTTCGG  >  NC_014034/3042855‑3043051

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG