Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,068,163 A→T 4.3% intergenic (‑146/+146) glcD ← / ← RCAP_rcc02872 glycolate dehydrogenase, subunit GlcD/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,068,1630A→T 4.3% 8.9 ‑1.7 ‑0.2138intergenic (‑146/+146)glcD/RCAP_rcc02872glycolate dehydrogenase, subunit GlcD/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (86/46):  total (92/46)
Fisher's exact test strand distribution p-value = 1.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 7.06e-01

Only 100 of 180 total aligned reads displayed.

ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263
                                                                                                     |                                                                                                        
aTTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCa                                                                                                          <  1:842149/101‑1 (MQ=255)
  tCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAgc                                                                                                        <  2:1320955/101‑1 (MQ=255)
  ctgcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAgc                                                                                                        <  1:2511930/99‑1 (MQ=255)
   cgcgCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAgcg                                                                                                       <  1:1033073/101‑1 (MQ=255)
    gcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAgcgc                                                                                                      <  2:2216653/101‑1 (MQ=255)
    gcgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAgcag                                                                                                      >  2:2511756/1‑99 (MQ=255)
     cgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGcgcc                                                                                                     >  2:682427/1‑101 (MQ=255)
     cgcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGcgcc                                                                                                     >  2:3258107/1‑101 (MQ=255)
      gcAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGcgccc                                                                                                    >  1:4606424/1‑101 (MQ=255)
        aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGcgcccgc                                                                                                  >  2:3264382/1‑101 (MQ=255)
        aaCCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGcgccccc                                                                                                  >  1:3946962/1‑99 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGcccgcccc                                                                                               <  1:986640/101‑1 (MQ=255)
           cGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGcccgcccc                                                                                               <  1:158099/101‑1 (MQ=255)
            gAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGcccgccccc                                                                                              >  1:192724/1‑100 (MQ=255)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCcgccccgccc                                                                                           <  1:1017780/101‑1 (MQ=255)
               aCGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCcgccccgccc                                                                                           <  1:2235110/101‑1 (MQ=255)
                 gATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                         >  2:88169/1‑101 (MQ=255)
                 gATGGGCAGGCTGACCGACGCCCTCGCCGAGGTCATGCGGAACAGTGGCGTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCAt                                                                                         <  2:517154/101‑1 (MQ=255)
                   tGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATgg                                                                                       >  2:1844685/1‑101 (MQ=255)
                     ggCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATggc.g                                                                                    <  2:4437711/101‑1 (MQ=255)
                     gcaaGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATggc.g                                                                                    >  2:1369109/4‑101 (MQ=255)
                        aGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGGCCCGCCCATggc.gggc                                                                                 >  1:1535491/1‑101 (MQ=255)
                          gCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGgcg.c                                                                              <  2:1603472/101‑1 (MQ=255)
                           cTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.Ct                                                                             <  1:1092964/101‑1 (MQ=255)
                           cTGACCGAGGCCAGGGCGGAGGTCGTGCGCAACTGGGGCCTCATGCCCGCCGAGGGCCTAAAGGCCCTC.GGGCAGGGCCCGCCGCGCCCATGGC.GGGCG.Ct                                                                             <  2:2025190/101‑1 (MQ=9)
                             gACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGACCGCCCCGCCCATGGC.GGGCG.CTtc                                                                           >  2:36781/1‑101 (MQ=255)
                              aCCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTtct                                                                          <  2:2174552/101‑1 (MQ=255)
                               ccGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTtctc                                                                         <  1:4670648/101‑1 (MQ=255)
                                cGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGGG.CCTCTcg                                                                        >  2:987639/1‑101 (MQ=255)
                                  ggcccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcc                                                                      <  1:2393825/98‑1 (MQ=255)
                                  aGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcc                                                                      <  1:1369204/101‑1 (MQ=255)
                                   ggCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgccc                                                                     <  2:4442822/101‑1 (MQ=255)
                                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccg                                                                    <  1:3480189/101‑1 (MQ=255)
                                    gCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATCGC.GGGGG.CCTCTcccccc                                                                    >  1:929017/1‑100 (MQ=18)
                                     ccATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgc                                                                   >  2:3014188/1‑101 (MQ=255)
                                      cATCGCCGAGGGCATGCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTC.TGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTcgcccgcc                                                                  <  2:2385829/101‑1 (MQ=255)
                                       aTCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCccgcc.g                                                                >  2:2811834/1‑101 (MQ=255)
                                        tCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.gg                                                               <  1:2161992/101‑1 (MQ=255)
                                         tgCCGAGGCCAATCGCAACAGAGGTCCTGTGACCGCCGAGGGCCCAAAGGCACTC.GGCCTGCCCCCGCCCCGCCCTCCGCGGGGCC.CCTCCcgcccgcccc                                                                >  2:4010428/2‑99 (MQ=21)
                                         cGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.ggg                                                              <  2:2250835/101‑1 (MQ=255)
                                          gCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.gggg                                                             <  2:2474002/101‑1 (MQ=255)
                                           ccGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.Ggggc                                                            <  2:4100751/101‑1 (MQ=255)
                                            cGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.Cgggcg                                                           >  2:3863204/1‑101 (MQ=255)
                                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.Ggggcgggc                                                        <  1:4444452/101‑1 (MQ=255)
                                               ggTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.Ggggcgggc                                                        <  2:4521759/101‑1 (MQ=255)
                                                gTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGGG.CTTCTCGCCCCCC.Ggggcggggg                                                       >  1:318053/1‑99 (MQ=255)
                                                 tCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCCCCCCCC.GGGGGGGgggc                                                      >  1:3163333/1‑101 (MQ=18)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGGCCGCC.GGGGCGGGCGc.c                                                    >  1:4510652/1‑101 (MQ=255)
                                                  cATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGc.c                                                    >  1:1292018/1‑101 (MQ=255)
                                                   aTGCGGAACAGTGGCCTCGTGACCGCCCAGGGCCCAAAGGCCCTC.GGCCAGCCCCCGCCCCGCCCATGGC.GGGCT.CTTCTCGCCCGCC.GGGGCGGGGGC.Cg                                                   >  2:3116385/1‑101 (MQ=17)
                                                     gCGGAACAGTGGCCTCGTGCCCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGc                                                 <  2:3490759/101‑1 (MQ=255)
                                                     gCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCCCCCCGCCCATGGC.GGGGG.CTTTTCGGCCGCC.GGggcgggcgtccgc                                                  >  1:2075691/1‑96 (MQ=17)
                                                      cgcgaCATTGGCCCCATGACCGCCGGGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.GTTCTCCCCCGCCGGGGGCGGGGCC.CGGc                                                 >  2:259607/5‑101 (MQ=17)
                                                      cGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGcc                                                >  1:4270656/1‑101 (MQ=255)
                                                      cGGAACAGTGGCCTCGGGCCCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGcc                                                <  1:1975985/101‑1 (MQ=255)
                                                       ggAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGccc                                               >  1:3682157/1‑100 (MQ=255)
                                                         aaCCGTGCCCGCGGGCCCGCCGAGGGCCCAAAGGCCCTC.GCCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCctct                                             <  2:1288729/101‑1 (MQ=14)
                                                         aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.Cgggccct                                             >  2:4470190/1‑96 (MQ=255)
                                                         aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGCccct                                             >  1:2560287/1‑101 (MQ=255)
                                                         aaCAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCCCC.GGGGCGGGGGG.CGGCctcc                                             >  1:2899676/1‑100 (MQ=255)
                                                           cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGcccccgc                                           >  1:1121434/1‑96 (MQ=255)
                                                           cAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CCTCTCGCCCCCC.CGGGCGGGGGC.Cgggccccgc                                           >  1:3795759/1‑94 (MQ=17)
                                                            attGGCCCCGTGACCGCCGAGGGCCCAGAGGCCCTC.GGCCTGCGCCCGCCCCGCCCATGGC.GGGCGCCTTCGCCCCGCCC.GGGGGGGGGGC.Cgggcccccc                                           >  1:3502596/3‑94 (MQ=14)
                                                              tgcccgcGTGCCCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCtgctgc                                        <  2:1971232/95‑1 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGGCCCtgctgc                                        >  1:4281483/1‑101 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGG.CGGCCCCcgctgc                                        >  2:1168812/1‑101 (MQ=255)
                                                              tGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.CGGGCGGGGGC.CCGGCCCtcctgc                                        >  2:1889618/1‑101 (MQ=14)
                                                               ggCCCCGTGACCGCCGAGGGCCCAGAGGCCCTC.GGCCTGCGCCCGCCCCGCCCATGGC.GGGCGCCTTCGCGGCGCCC.GGGGGGGGCGCGCGGccccccccc                                         >  1:3905482/1‑94 (MQ=11)
                                                               ggCCCCGTGACCGCAGAGGGCCAAAAGCCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTgcg                                       <  1:4658254/101‑1 (MQ=14)
                                                                 ccTCGTGCCCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGcc                                     <  1:1443431/101‑1 (MQ=255)
                                                                    cGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.CGGGCGGGGGC.CGGCCTCTGCTGCGCCCgg                                  >  2:1065322/1‑101 (MQ=255)
                                                                    cGTGACCGCCGAGGGCCCAAAGGCACTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CCGCCCctgctggccccgg                                  >  2:2107314/1‑94 (MQ=14)
                                                                     gTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGCCCCCGCTGCGCCTggg                                 >  2:3745336/1‑101 (MQ=255)
                                                                     gTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CCGCCCCTGCTTCGCCTggg                                 >  2:4647258/1‑101 (MQ=255)
                                                                     gTGACCCCCGAGTGCCCAAA.GCACTC.GGGCAGCGCCCGCCCCGCCCCTGGC.GGCCG.CTTCCCGCCCGGC.GGGGGCGGGGG.CGGCCCCGCATGCCCCTgggg                                >  2:3894852/1‑101 (MQ=2)
                                                                      tGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.CGGGCGGGGGC.CGGCCCCTGCTGCGCCTgggg                                >  1:1836682/1‑101 (MQ=255)
                                                                        ccggccgGGGCCCCAAGGGCCCCG.GGCCCGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGaa                              <  1:3014417/98‑1 (MQ=14)
                                                                        cccgccgAGGGCCCAAAGGCCCTC.GGCCTGCGCCCGCCCCGCCATCGGCGGGGGG.CGTCGGGCCCCCC.GGGGGGGGGGG.GCGCGCCTGGTGCGCGTggggg                               >  1:2266289/2‑100 (MQ=2)
                                                                          cgccgAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGGAAc                            >  1:3194271/1‑101 (MQ=255)
                                                                           gccgAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGGGGGCGC.CGGCCTCTGCCGCGCCCGGGGGAACg                           >  2:2921410/1‑101 (MQ=14)
                                                                           gccgAGGGCCCAAAGCCCCCC.GCCCGGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACg                           <  1:3745611/101‑1 (MQ=17)
                                                                              gAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGGGC.CGGCCTCTGCTGCGCCCGGGGGAAAgggc                        >  2:2847325/1‑99 (MQ=14)
                                                                              gAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGG.CCGCCCCTGCTGCGCCTTGGGGAACgggc                        >  1:1891963/1‑99 (MQ=14)
                                                                                ggccccAAGGGCCCGC.GGCCGGGGCCCGCCCCGCCCAGGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCtt                      <  1:3253589/98‑1 (MQ=17)
                                                                                   cccAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGAGC.CGGCCTCCGCTGCGCCCGGGGAAACggggtgggc                   >  1:1162120/1‑94 (MQ=14)
                                                                                    ccAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGGAACGGTCTTGGCa                  >  2:653584/1‑101 (MQ=255)
                                                                                    ccAAAGGCCCCCGGGCCGGCGCCCGCCCCGCCCAGGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGc                   <  1:1735974/101‑1 (MQ=17)
                                                                                     cAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCCGGGGGAACGGGCtttggcc                 >  2:1721011/1‑96 (MQ=14)
                                                                                      aaaGCCCCTC.GCCCAGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCAcc                <  2:109345/101‑1 (MQ=255)
                                                                                       aaGGCCCTC.GGCCAGCGCCCGCCCCGCCCATTGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCCCCTGCGCCTCGGGAAACCGGCTTgggcccg               >  2:798112/1‑96 (MQ=14)
                                                                                        aGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGGCTTGGCACCGc              >  2:2041847/1‑101 (MQ=255)
                                                                                           cccGC.GGGCTGCGCCCGCCCCCCCCATGGC.GGGCG.CTTGTGGCCCCCG.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCg           <  1:1548664/101‑1 (MQ=17)
                                                                                             cTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCCCCCggg         >  1:753277/1‑101 (MQ=255)
                                                                                             cTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCCCC.CGGGCGGGCGC.CGGGCTCTGCTGCGCCCGGGGGAACGGTCTTTGCACCGCCCcgg         >  1:1627921/1‑101 (MQ=18)
                                                                                               cgggCCGGCCCCCCCCCCCCCCTGGGC.GGGCG.CTTCCCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGc        <  1:2934073/99‑1 (MQ=18)
                                                                                                 ggCCGGCCCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGGGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTc      <  2:2290528/101‑1 (MQ=255)
                                                                                                 gcccAGCCCCCCCCCGCCCCTTGGC.GGGCG.CTTCCCGCCCGCG.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTc      <  2:1448801/99‑1 (MQ=18)
                                                                                                   ccAGCGACCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcg    <  1:36787/101‑1 (MQ=255)
                                                                                                    cAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg   >  1:1271358/1‑101 (MQ=255)
                                                                                                    cAGCCCCCGCCCCCCCCATGGC.GGGCG.CTCCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCcgg   <  2:2350348/101‑1 (MQ=255)
                                                                                                     |                                                                                                        
ATTCGCGCAACCGAAACGATGGGCAGGCTGACCGAGGCCATCGCCGAGGTCATGCGGAACAGTGGCCTCGTGACCGCCGAGGGCCCAAAGGCCCTC.GGCCAGCGCCCGCCCCGCCCATGGC.GGGCG.CTTCTCGCCCGCC.GGGGCGGGCGC.CGGCCTCTGCTGCGCCTGGGGAAACGGTCTTGGCACCGCCCGGGCTCCGGC  >  NC_014034/3068063‑3068263

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG