Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,223,935	G→C	100%	P129A (CCC→GCC)	fdhD ←	formate dehydrogenase accessory protein FdhD

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,223,935	0	G→C	100.0%	60.0	16	P129A (CCC→GCC)	fdhD	formate dehydrogenase accessory protein FdhD
Reads supporting (aligned to +/- strand):  new base (2/14):  ref base (0/0):  total (2/14)

GCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGC  >  NC_014034/3223840‑3223954                                                                                                |                    gcCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcggggg                >  1:730514/1‑101 (MQ=255)  ccGTCCCAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  1:4210957/101‑1 (MQ=255)  ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  1:2122493/101‑1 (MQ=255)  ccGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  1:2357537/101‑1 (MQ=255)  ccGCCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgc               <  1:2581766/101‑1 (MQ=255)    tccccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgc             <  2:3163255/99‑3 (MQ=255)    gTCCCAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgc             <  2:3952528/101‑3 (MQ=255)     tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcc            <  2:3735003/101‑1 (MQ=255)     tCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgca            >  1:3163499/1‑98 (MQ=255)      cccAGACCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgcca           <  1:4048933/101‑2 (MQ=255)       ccAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaa          <  2:3321374/101‑3 (MQ=255)        cAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaag         >  1:3675285/1‑98 (MQ=255)         aGAGCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagc        <  2:4459280/101‑5 (MQ=255)           aaCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAggggggcgggggggccaagccc      >  1:1229547/1‑91 (MQ=255)            aCCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgcc     <  1:2047146/101‑8 (MQ=255)             ccccGCCGCATGCACGGACCGGACCGCATCNTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccc    <  1:1221171/101‑9 (MQ=255)              cccGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccag   <  1:1703148/101‑10 (MQ=255)               ccgccgCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGCGGGGgcgccaagcgccagc  <  1:208989/101‑11 (MQ=255)                                                                                                |                    GCCGTCCCAGAACCCCGCCGCATGCACGGACCGGACCGCATCCTGCAGGCTCTGCCCGGCCCGCAAGGCCGCAAGCGCACCATCGGCCAGGGGGGGGGGGGCCCCGCCCCGGGGC  >  NC_014034/3223840‑3223954 Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG