Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,685 C→A 100% intergenic (+10/‑173) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6850C→A100.0% 548.1 150intergenic (+10/‑173)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (33/113):  ref base (2/1):  total (35/115)

Only 100 of 155 total aligned reads displayed.

CCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAA  >  NC_014034/3254588‑3254776
                                                                                                  |                                                                                           
 ccGAAATCCATCGTAGCCTAAACGCTCCTGATGCGCTACAAACCCGGCGCGGTCCGCCGGGTGATCTATCGCATCCCGATCCGCTGAGGCAACGCT.ACggg                                                                                         <  2:3121221/101‑1 (MQ=255)
  cGAAATTCATCGAGTCCGAAACGATCCTGATGCCCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggg                                                                                        <  1:4027666/101‑1 (MQ=255)
  cGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggg                                                                                        <  2:4115501/101‑1 (MQ=255)
  cGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcggg                                                                                        >  2:3525983/1‑101 (MQ=255)
   gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                       >  2:3877922/1‑101 (MQ=255)
   gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                       <  2:3997611/101‑1 (MQ=255)
    aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcggggg                                                                                      >  2:584067/1‑101 (MQ=255)
     aaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggg                                                                                      >  1:2101127/1‑101 (MQ=255)
     aaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggac                                                                                     <  2:1687040/101‑3 (MQ=255)
      aTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggact                                                                                    <  2:4216022/101‑4 (MQ=255)
       ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactg                                                                                   <  2:1708973/101‑5 (MQ=255)
        tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgc                                                                                  >  2:2602623/1‑96 (MQ=255)
         cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgcc                                                                                 <  1:3010909/101‑7 (MQ=255)
          aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggcgg                                                                                 >  1:1269643/1‑96 (MQ=255)
           tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgt                                                                               <  1:1971453/101‑9 (MQ=255)
           tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgt                                                                               <  1:2135366/101‑9 (MQ=255)
           tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgt                                                                               <  2:3414641/101‑9 (MQ=255)
            cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgtc                                                                              <  1:669653/101‑10 (MQ=255)
             gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACcgggggcgggcggcc                                                                             >  1:3449111/1‑92 (MQ=255)
             gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACaggggggggggctgc                                                                             >  2:4040784/1‑92 (MQ=255)
               gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTcccc                                                                          <  2:550802/101‑1 (MQ=255)
                 ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                        <  2:3816587/101‑1 (MQ=255)
                 ccGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCg                                                                        <  1:3350768/101‑1 (MQ=255)
                      aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccc                                                                   <  1:4470561/101‑1 (MQ=255)
                        gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCCGCCCCCCCCCccccc                                                                 >  1:3487844/1‑101 (MQ=255)
                        gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.CGGCCGTCCCCCCCCccccc                                                                 >  2:2218721/1‑101 (MQ=255)
                        gATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCAccccc                                                                 <  1:4330502/101‑1 (MQ=255)
                         aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGGGGGGCCGCCCCCCCCCccccc                                                                 >  2:1499475/1‑101 (MQ=255)
                         aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCt                                                                <  1:271363/101‑1 (MQ=255)
                         aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCt                                                                <  2:1969332/101‑1 (MQ=255)
                         aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCt                                                                <  2:4216991/101‑1 (MQ=255)
                          tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                               <  2:4542852/101‑1 (MQ=255)
                          tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                               <  2:2411971/101‑1 (MQ=255)
                          tCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTg                                                               <  1:2847010/101‑1 (MQ=255)
                           ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCAGGGGGCAAGCGCCCCCCCCCCcccccc                                                                >  1:2613409/1‑100 (MQ=255)
                           ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgc                                                              <  2:447101/101‑1 (MQ=255)
                             tGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAGCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTgcgc                                                            <  1:2914053/101‑1 (MQ=255)
                              gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGcgcg                                                           <  1:659342/101‑1 (MQ=255)
                               aTGCGCGCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGt                                                          <  2:247362/101‑1 (MQ=255)
                                 gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAt                                                        <  1:440554/101‑1 (MQ=255)
                                 gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAt                                                        <  1:584128/101‑1 (MQ=255)
                                    cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                     <  2:2152859/101‑1 (MQ=255)
                                    cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                     <  2:1160657/101‑1 (MQ=255)
                                    cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                     <  2:1344829/101‑1 (MQ=255)
                                    cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTAtttt                                                     <  2:1431876/101‑1 (MQ=255)
                                      cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGc                                                   <  2:2029194/101‑1 (MQ=255)
                                      cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGc                                                   <  2:1269550/101‑1 (MQ=255)
                                       gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCCCACCCCCCGCGCCGATTTTGcc                                                  >  2:1330090/1‑101 (MQ=255)
                                        aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                 <  1:2779244/101‑1 (MQ=255)
                                        aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                 <  1:4569194/101‑1 (MQ=255)
                                        aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCa                                                 <  2:3909098/101‑1 (MQ=255)
                                         aaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAGCGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCaa                                                <  2:2095972/101‑1 (MQ=255)
                                          aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAg                                               <  1:1087342/101‑1 (MQ=255)
                                              ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                           <  1:1499584/101‑1 (MQ=38)
                                              ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaa                                           <  1:4042744/101‑1 (MQ=38)
                                               gcccGGTGCGCCGGGTTATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                          <  1:3697100/98‑1 (MQ=37)
                                               gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                          <  2:1027475/101‑1 (MQ=38)
                                               gCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGaaaa                                          <  1:2325939/101‑1 (MQ=38)
                                                 tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACg                                        <  2:1123846/101‑1 (MQ=38)
                                                    gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGTCCCCCGCACCCCCCCCGCGGCTTTTTCCCAGGAAAACgggg                                     >  1:1343865/1‑101 (MQ=255)
                                                    gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACgggg                                     <  2:1793684/101‑1 (MQ=38)
                                                      gcgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGcc                                   <  2:17358/101‑1 (MQ=38)
                                                       cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                  >  2:712934/1‑101 (MQ=38)
                                                       cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                  <  1:2916078/101‑1 (MQ=38)
                                                       cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCg                                  <  1:3188547/101‑1 (MQ=38)
                                                         ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGCGGGG.CCGGCGCCCCCCCCACCCCCCTCGCCGAGTTTGGCCACGAAAAAGGGGGCgct                                >  1:1528656/1‑99 (MQ=255)
                                                         ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACggggacaac                                <  2:2440448/101‑6 (MQ=38)
                                                         ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGtt                                <  1:147587/101‑1 (MQ=38)
                                                           gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGCCGCCCCCCCCCCCCCCCCCGCGCGTTTTTCCAAGGAAAAAAGGGCCGCTTc                              >  1:3089753/1‑101 (MQ=255)
                                                            ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCa                             <  1:1192337/101‑1 (MQ=38)
                                                             gTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGTCCCCCCCCCCCCCCGCGCGGCTTTTTTCAAGGAAAAAAGGGCCGGTTCAc                            >  2:2663691/1‑101 (MQ=255)
                                                              tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGCGGCCCCCCCCCCCCCCCCCCGGGTTTTTTCCCAGGAAAAAGGGGcgggtttcc                            >  1:1152305/1‑93 (MQ=255)
                                                               gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGCCGGCCCCCGCACCCCCCGCGCGGATTTTGCCAAGGAAAACAGGGGCGGTTTACCt                          >  2:2931137/1‑101 (MQ=21)
                                                                aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                         <  1:390289/101‑1 (MQ=38)
                                                                aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                         <  1:2660925/101‑1 (MQ=38)
                                                                aTCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                         <  2:1728282/101‑1 (MQ=38)
                                                                aTCTATCGCAACACGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCtt                         <  2:3898496/101‑1 (MQ=38)
                                                                 tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                        <  2:281610/101‑1 (MQ=38)
                                                                 tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                        <  2:2732809/101‑1 (MQ=38)
                                                                 tCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTg                        <  2:4287794/101‑1 (MQ=38)
                                                                  cTATCGCAACCCGATCCGCTGAGGCAACGCT.GC.GGGGGACTGCCGTCCCCCGCACCCCCTTCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTgg                       <  2:2320342/101‑1 (MQ=38)
                                                                  cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTgg                       <  1:1673224/101‑1 (MQ=38)
                                                                    aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGcc                     <  2:3679341/101‑1 (MQ=38)
                                                                     tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                    <  1:2303984/101‑1 (MQ=38)
                                                                     tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCg                    <  2:106369/101‑1 (MQ=38)
                                                                      cNNAACACGATCCGCTGNGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                   <  2:1908779/101‑1 (MQ=37)
                                                                      cGCACCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGa                   <  2:3286535/101‑1 (MQ=38)
                                                                        cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGaaa                 <  2:4242971/101‑1 (MQ=38)
                                                                         aaCCCGATCCGCTGAGGNAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                <  2:387979/101‑1 (MQ=38)
                                                                         aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAat                <  1:4568001/101‑1 (MQ=38)
                                                                          aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCCCCCCCCGCGCGTATTTTGCCAAAGACAACGGGGGCGGTTCACCTTGGCCGACata               >  2:2094577/1‑101 (MQ=255)
                                                                          aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAata               <  1:3526241/101‑1 (MQ=38)
                                                                          aCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAata               <  1:4622562/101‑1 (MQ=38)
                                                                             cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATcc            <  2:2029436/101‑1 (MQ=38)
                                                                              gATCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATccc           <  1:4503256/101‑1 (MQ=38)
                                                                               aTCCGCTGAGGCAACGCT.ACGGGGGG.GTGCCGGCCCCCCCCCCCCCCCCCCGGCTTTTTTCAAAGAAAAAGGGGGCCGTTCACCCTTGCCCAAAAATcccc          >  1:3874183/1‑100 (MQ=255)
                                                                               aTCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAGATATCCCg          <  1:1371091/101‑1 (MQ=38)
                                                                               aTCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCg          <  1:4342452/101‑1 (MQ=38)
                                                                                tCCGCTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgg         <  2:3822633/101‑1 (MQ=38)
                                                                                    cTGAGGCAACGCT.AC.GGGGGACTGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCgggggg     <  1:3896625/101‑1 (MQ=38)
                                                                                                  |                                                                                           
CCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGCCGTCCCCCGCACCCCCTGCGCGTATTTTGCCAAGGAAAACGGGGCCGTTTCACCTTGGCCGAAATATCCCGGGGGGGAA  >  NC_014034/3254588‑3254776

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG