Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	3,333,494	C→G	100%	intergenic (+40/‑126)	ccmH → / → paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	3,333,494	0	C→G	100.0%	78.7	21	intergenic (+40/‑126)	ccmH/paaG	cytochrome c‑type biogenesis protein CcmH/enoyl‑CoA hydratase
Reads supporting (aligned to +/- strand):  new base (0/21):  ref base (0/0):  total (0/21)

GGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTC  >  NC_014034/3333397‑3333579                                                                                                  |                                                                                      ggtgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGgggggc                                                                                    >  2:2194187/1‑101 (MQ=255)  gtgccggtgcTCTCGGCCGCGGAAGAGGCGCGGCTGAAAGCCCTGCTGAAGGCCTAGGGATTCCGCCCGGGGCTTGCGCCCCGGGCCACCCGCGGGGGGCt                                                                                   <  2:2438743/101‑1 (MQ=255)   tgccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTc                                                                                  <  1:2678639/101‑1 (MQ=255)    gccggtgcTCTCGGCCGAGGAAGGGGCGCGGCTGAAAGACCTGCTGAAGGACTGGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                 <  2:3282473/101‑1 (MQ=255)    gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                 <  2:3613427/101‑1 (MQ=255)    gccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                 <  2:2446785/101‑1 (MQ=255)    gccggggcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTcc                                                                                 <  1:1631908/101‑1 (MQ=255)     ccggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCg                                                                                <  2:4425690/101‑1 (MQ=255)      cggtgcTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGGGACCCgccgccggggcggc                                                                               >  2:1124681/1‑95 (MQ=255)           ctctCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                          <  1:3799881/101‑1 (MQ=255)           cgctcGGCCGAGGAAGAGGGGCGGTTGAAAGACCTGCTGAAGGACTAGGGATTTCGCGCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGcccccc                                                                          <  2:217951/99‑1 (MQ=38)            tctcGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCg                                                                         <  2:3339563/101‑1 (MQ=255)               cGGCCGAGGAAGCGGCGGCGCTGACAGACGTGCGGAAGGACTAGGGTTTTCGCCCGGGGCTTGCGGCCCGTGGGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                      <  2:2077895/101‑1 (MQ=25)               cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCTCCCCCCGCAc                                                                      <  2:3921656/101‑1 (MQ=255)               cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                      <  2:4236954/101‑1 (MQ=255)               cGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAc                                                                      <  2:2903414/101‑1 (MQ=255)                ggCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcc                                                                     <  1:2194337/101‑1 (MQ=255)                    gAGGAAGAGGCGCGGCTGAAAGCCCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCAcccccc                                                                 <  2:828824/101‑1 (MQ=255)                      ggAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCgg                                                               <  2:652561/101‑1 (MQ=255)                               gcgGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGGGCCGGCCCCCGCGCCCCCCcgggccacctt                                                      >  2:4416214/1‑93 (MQ=255)                                               ctgAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCggg                                      <  2:3079237/101‑1 (MQ=255)                                                                      ggggCTTGCGCCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGttt               <  1:1381754/101‑1 (MQ=255)                                                                             gcgcCCCGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTg        <  1:4416506/101‑1 (MQ=255)                                                                                   cGGGCGACCCGCGGGGGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTc  <  2:76399/101‑1 (MQ=255)                                                                                                  |                                                                                      GGTGCCGGTGCTCTCGGCCGAGGAAGAGGCGCGGCTGAAAGACCTGCTGAAGGACTAGGGATTTCGCCCGGGGCTTGCGCCCCGGGCGACCCGCGGGCGGCTCCGCCCCCCGCACCCCCCGGGCGTATTTTGCCAAGGAAAAGCGGGGGTCTGGTCGTGACGTTTGGTTTCGCTTTGCCTTTC  >  NC_014034/3333397‑3333579 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 39 ≤  ATCG