Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,596,459 C→G 100% G470G (GGC→GGG)  RCAP_rcc03391 → type I secretion system ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,596,4590C→G100.0% 742.3 199G470G (GGC→GGG) RCAP_rcc03391type I secretion system ATPase
Reads supporting (aligned to +/- strand):  new base (96/103):  ref base (0/0):  total (96/103)

Only 100 of 220 total aligned reads displayed.

CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556
                                                                                                  |                                                                                                 
cccgaGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGcgggcg                                                                                                 <  1:1928924/101‑1 (MQ=255)
   tctatgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGct                                                                                              <  2:526081/98‑1 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGct                                                                                              <  1:3667721/101‑1 (MQ=255)
   gagaTGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCCGATCCTCGATCTGCCCGAGGGATATGACAACCGGGGTTCGCAGGCGGGCGGGCGGct                                                                                              >  2:745923/1‑101 (MQ=255)
     gatgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGctct                                                                                            <  2:1572780/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           >  1:2865202/1‑101 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           <  1:3531316/101‑1 (MQ=255)
      atgatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCtctc                                                                                           <  1:3707034/101‑1 (MQ=255)
        gatCGTCGATGCGGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGGGGGCGGCTCTcgg                                                                                         >  1:2530892/1‑101 (MQ=255)
        gatCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgg                                                                                         <  1:2796496/101‑1 (MQ=255)
          tcgtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggc                                                                                       <  2:3641878/101‑1 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTcgggcgg                                                                                     >  2:2574263/1‑101 (MQ=255)
            gtcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcgg                                                                                     >  2:2612667/1‑101 (MQ=255)
             tcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcggg                                                                                    <  1:3469189/101‑1 (MQ=255)
             tcgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcggg                                                                                    <  2:3695783/101‑1 (MQ=255)
             gtgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTcgggcggg                                                                                    >  2:3051206/3‑101 (MQ=255)
              cgATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTCgggcgggc                                                                                   >  2:4336825/1‑101 (MQ=255)
               gATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCa                                                                                  <  2:873706/101‑1 (MQ=255)
                 tGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCCCTCGGGGGGGCAGa                                                                                >  1:60731/1‑101 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                             <  2:3124509/101‑1 (MQ=255)
                    cgcgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATcc                                                                             >  1:2229652/1‑101 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                           <  2:4124949/101‑1 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAg                                                                           <  2:2901882/101‑1 (MQ=255)
                      cgAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCCCCCGGGCGGGCAGATCCAg                                                                           >  1:2900907/1‑101 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAgcg                                                                         <  1:272223/101‑1 (MQ=255)
                        aaaCGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCACATCCCgcc                                                                         >  1:3800062/1‑100 (MQ=255)
                           cGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGt                                                                      <  2:1867633/101‑1 (MQ=255)
                             ggcggcggcGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCg                                                                    <  1:1847243/101‑1 (MQ=255)
                             ggcggcggcGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCg                                                                    <  1:2044887/101‑1 (MQ=255)
                             ggcggcggaGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGGTTGCCGGCGGGCAGAGCCAGggcgggc                                                                    >  2:4404023/1‑98 (MQ=255)
                                ggcggcgCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGTCGGGGGGCTATCGGGGGGGGAGACACCACGCGCCGGcc                                                                 >  2:3913768/1‑101 (MQ=255)
                                    gcgcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGc                                                             <  1:1864791/101‑1 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           >  1:3492547/1‑101 (MQ=255)
                                      gcATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGccc                                                           <  2:1498680/101‑1 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCCGCGCGTCGGCCTGGCCCg                                                          >  2:2412516/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                          >  1:672213/1‑101 (MQ=255)
                                       cATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCg                                                          <  2:2724269/101‑1 (MQ=255)
                                        aTCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCgg                                                         >  1:867007/1‑101 (MQ=255)
                                         tCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCggg                                                        <  2:1114011/101‑1 (MQ=255)
                                            gCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGGGGCTCTCGGGCGGGCAGAGCCCGCGCGACGGGCCGGGCCGGgggc                                                     >  2:4396067/1‑101 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                    <  2:920552/101‑1 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCt                                                    <  2:866931/101‑1 (MQ=255)
                                             cTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCCCCGGCGGGCAGGTCCAGCGCGTCGGCCCGGCCCGGGCGCt                                                    >  2:811998/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   >  1:4444826/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   >  1:3214238/1‑101 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   <  1:1708421/101‑1 (MQ=255)
                                              tGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCtt                                                   >  1:1442924/1‑101 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATTACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTa                                                 <  2:784309/101‑1 (MQ=255)
                                                aTCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTa                                                 >  2:4455318/1‑101 (MQ=255)
                                                    tCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGc                                             >  1:1619858/1‑101 (MQ=255)
                                                     cGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGGCCGGGCGCTTTACGGCa                                            >  1:989398/1‑101 (MQ=255)
                                                     cGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCa                                            <  1:2600870/101‑1 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGGCCTCGGGCGGGCAGATCCAACGCGTCGGCCTGGCCCGGGCGCTTTTCGCCAAc                                          >  1:56484/1‑101 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAc                                          <  1:1305176/101‑1 (MQ=255)
                                                       aTCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAc                                          <  2:2790275/101‑1 (MQ=255)
                                                         tcgCCCGAGGGATATGACCCCCGGGTTTCGGAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        <  2:922542/99‑1 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCTGCGCCTCGGCCTGGCCCCGGCGCTTTACGGCAAacc                                        >  1:536938/1‑101 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCCAccc                                        >  2:4365458/1‑101 (MQ=255)
                                                         cTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAAccc                                        >  1:4631794/1‑101 (MQ=255)
                                                          tGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCg                                       <  1:1099152/101‑1 (MQ=255)
                                                           gCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgg                                      <  2:2639128/101‑1 (MQ=255)
                                                            cccGAGGGATATGACCCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCCTTACGGCAACCCGGt                                     <  1:3931327/101‑1 (MQ=255)
                                                            cccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTTTCGGGCGGGCAGATCCAGCGCGGCGGCCTGGCCCGGGCGCTTTACGGCAACCCggg                                     >  2:4567861/1‑100 (MQ=255)
                                                             ccGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCgggg                                    >  1:3944258/1‑99 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGGGCTTTTCGGGAACCCGGggct                                  >  1:3200786/1‑101 (MQ=38)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  >  2:4204806/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgct                                  >  2:3504568/1‑101 (MQ=255)
                                                               gAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCCGtgct                                  >  1:4174465/1‑101 (MQ=255)
                                                                aGGGCTATGACACCCGGGTTTCCCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCGGCGCGCCGGCCTGGCCCGGGGGCGTTACGGGCACCCGGcgctc                                 >  2:1409265/1‑100 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                 <  1:3718813/101‑1 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                 <  1:4494870/101‑1 (MQ=255)
                                                                aGGGATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctg                                 <  1:1655983/101‑1 (MQ=255)
                                                                 gggATATGACTCCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctgc                                <  1:3891807/101‑1 (MQ=255)
                                                                  ggATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTgctgct                               >  2:1309457/1‑101 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGtgctgccg                              >  2:3821558/1‑99 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGctgctg                              <  1:993777/101‑1 (MQ=255)
                                                                   gATATGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGctgctg                              <  2:2158683/101‑1 (MQ=255)
                                                                    atatGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTgg                             <  2:514853/101‑1 (MQ=255)
                                                                     tatGACACCCGGGTTTCGCAGGCGGGCGGGCGGGTCTCGGGCGGGCAGATCCAGCCCGTCGGCCTTGCCCNGGTGGTTTACCGCAACCCGGTGCTGCCggg                            >  1:1229784/1‑100 (MQ=255)
                                                                      atGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTg                           <  2:2976983/101‑1 (MQ=255)
                                                                       tGACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGc                          >  2:2070027/1‑101 (MQ=255)
                                                                        gACACCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCt                         <  1:1527384/101‑1 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                       <  2:1725817/101‑1 (MQ=255)
                                                                          cacCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcg                       <  2:3396137/101‑1 (MQ=255)
                                                                           ccccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcga                      <  2:3295498/100‑1 (MQ=255)
                                                                           acCCGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCCGCGCGTCGGCCTGGCCCGGGCGCTTTTCGGCAACCCGGGGCTGCTGGTGGTcga                      >  1:1970056/1‑101 (MQ=255)
                                                                            cccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCCGGCGCTTTACGGCAACCCCGTGCTGCTGGTGCCcgac                     >  2:4061381/1‑101 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  2:3227221/101‑1 (MQ=255)
                                                                             ccGGGTTTCGCAGGCGGGCGGGCGGCTCTCGAGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTcgacg                    <  1:3269583/101‑1 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  1:4568160/101‑1 (MQ=255)
                                                                               gggTTTCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAg                  <  1:2435208/101‑1 (MQ=255)
                                                                                  tttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCg               <  1:2753002/101‑1 (MQ=255)
                                                                                  tttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCg               >  1:1135587/1‑101 (MQ=255)
                                                                                   ttCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTagatcggaaga              >  1:964486/1‑93 (MQ=38)
                                                                                    tCGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGaa             >  1:1031813/1‑101 (MQ=255)
                                                                                     cGCAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAAt            >  1:4429952/1‑101 (MQ=255)
                                                                                       cAGGCGGGCGGGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGc          >  2:1803251/1‑101 (MQ=255)
                                                                                           cgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAt      <  2:2308213/101‑1 (MQ=255)
                                                                                           cgggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAt      >  1:4281089/1‑101 (MQ=255)
                                                                                            gggcggGCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAAtc     >  1:2303540/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CCCGAGATGATCGTCGATGCCGCGAAACGGGCGGCGGCGCATCAGCTGATCCTCGATCTGCCCGAGGGATATGACACCCGGGTTTCGCAGGCGGGCGGCCGGCTCTCGGGCGGGCAGATCCAGCGCGTCGGCCTGGCCCGGGCGCTTTACGGCAACCCGGTGCTGCTGGTGCTCGACGAGCCGAATGCGAATCTCG  >  NC_014034/3596361‑3596556

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 23 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG