BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	539,743	T→G	100%	Y201D (TAC→GAC)	RCAP_rcc00505 →	type II secretion system protein E

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	539,743	0	T→G	100.0%	926.2	248	Y201D (TAC→GAC)	RCAP_rcc00505	type II secretion system protein E
Reads supporting (aligned to +/- strand): new base (140/108): ref base (0/0): total (140/108)

Only 100 of 258 total aligned reads displayed.


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840


cGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGAc                                                                                                 <  2:1179440/101‑1 (MQ=255)


 ggACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACg                                                                                                >  2:2070986/1‑101 (MQ=255)


 ggACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACg                                                                                                >  2:1779064/1‑101 (MQ=255)


   aCGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGcc                                                                                              <  1:1113628/101‑1 (MQ=255)


    cGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACGAGATCGTGTCCGCGGTGGGGCGCGGTGTCGATGAAAGCAACCCCTATGTCGACGccc                                                                                             >  1:3988584/1‑101 (MQ=255)


     gTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCg                                                                                            >  1:4454381/1‑101 (MQ=255)


      tGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGt                                                                                           <  2:1371421/101‑1 (MQ=255)


       gACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTc                                                                                          >  2:4106286/1‑101 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         <  1:2591662/101‑1 (MQ=255)


        aCCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCt                                                                                         >  1:3395240/1‑101 (MQ=255)


          cTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTgg                                                                                       >  1:2707825/1‑101 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGc                                                                                      >  2:2992649/1‑101 (MQ=255)


           ttCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGc                                                                                      >  1:2330917/1‑101 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     <  2:3296321/101‑1 (MQ=255)


            tCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGcc                                                                                     >  2:1657815/1‑101 (MQ=255)


             cAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCg                                                                                    <  1:3644811/101‑1 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  <  2:1624158/101‑1 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  >  1:1412564/1‑101 (MQ=255)


               aGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGAt                                                                                  >  2:154794/1‑101 (MQ=255)


                ggATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATg                                                                                 >  1:2030643/1‑101 (MQ=255)


                 gATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATgg                                                                                >  2:3377926/1‑101 (MQ=255)


                 gATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATgg                                                                                >  1:2157725/1‑101 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               <  1:7075/101‑1 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               >  2:4196114/1‑101 (MQ=255)


                  aTGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGCGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGc                                                                               <  1:3922368/101‑1 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              >  1:2305483/1‑101 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              <  1:2002535/101‑1 (MQ=255)


                   tGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCt                                                                              >  1:4578823/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  2:2985203/1‑101 (MQ=255)


                    gAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTc                                                                             >  1:4404085/1‑101 (MQ=255)


                     aaaaGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTcg                                                                            >  1:4331464/1‑101 (MQ=255)


                        aGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGt                                                                         >  1:2244292/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCTACCCCTATGTCGACGCCCGTCTGGCCGATGGCCCGCGTTTc                                                                      <  2:4829/101‑1 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGGGTTTc                                                                      >  1:2081597/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      >  1:45988/1‑101 (MQ=255)


                           aCCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTc                                                                      >  2:815952/1‑101 (MQ=255)


                            ccTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCa                                                                     <  2:2348913/101‑1 (MQ=255)


                             cTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCaa                                                                    >  1:4127381/1‑101 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   >  2:2586844/1‑101 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   <  1:1726195/101‑1 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   >  1:3499543/1‑101 (MQ=255)


                              tGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAc                                                                   >  2:429464/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  >  1:4077324/1‑101 (MQ=255)


                               gTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAAcg                                                                  <  2:1977003/101‑1 (MQ=255)


                                  gCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGAGACGATCAAAGCAACCCCTATGACGACGCCCGACTCGCCCAAGGCCCGCGCTCCAAcccac                                                               >  2:1748983/1‑97 (MQ=255)


                                   cGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGAt                                                              <  2:120495/101‑1 (MQ=255)


                                     gATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATgg                                                            >  1:1304647/1‑101 (MQ=255)


                                     gATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATgg                                                            >  2:3156585/1‑101 (MQ=255)


                                     gATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATgg                                                            >  2:2020496/1‑101 (MQ=255)


                                       tcatcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTg                                                          <  1:4387856/101‑1 (MQ=255)


                                        cttcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGc                                                         <  1:1012505/99‑1 (MQ=255)


                                        catcGACAAGATCGTGTCCGCGGTGGGGCGGCGGGTCGATGAAAGCAACGCCTAGGGCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGc                                                         <  2:2053941/101‑1 (MQ=255)


                                          tcGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcag                                                       >  2:1012422/1‑99 (MQ=255)


                                           cGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGcccc                                                      >  1:2960943/1‑101 (MQ=255)


                                           cGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGACTGGCCGATGGCTCGCGTTTCAACGCGagatcggaag                                                      >  1:3249836/1‑92 (MQ=255)


                                              cAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCgg                                                   >  1:425870/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  2:4410363/1‑101 (MQ=255)


                                               aaGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGt                                                  >  1:1940833/1‑101 (MQ=255)


                                                  aTCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcgc                                               >  2:2105596/1‑101 (MQ=255)


                                                   tCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcgcg                                              >  1:4190397/1‑101 (MQ=255)


                                                   tCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTcgcg                                              >  1:2452159/1‑101 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGt                                            >  1:902545/1‑101 (MQ=255)


                                                     gtgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGt                                            <  1:3344742/101‑1 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           >  1:3686598/1‑101 (MQ=255)


                                                      tgtCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTg                                           <  1:1145875/101‑1 (MQ=255)


                                                        tctgcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGa                                         <  1:3827500/98‑1 (MQ=255)


                                                          cgcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATg                                       <  1:1977042/101‑1 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGaaga                                      >  2:3827215/1‑98 (MQ=255)


                                                           gcgGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATgg                                      <  1:586091/101‑1 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   <  2:451184/101‑1 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   >  2:4245493/1‑101 (MQ=255)


                                                              gTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctc                                   <  2:1165346/101‑1 (MQ=255)


                                                                  ggcggcGTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGgctcgctc                               <  1:2992878/101‑1 (MQ=255)


                                                                    cggcgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGt                             >  1:94518/1‑101 (MQ=255)


                                                                       cgTGTCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGtctc                          <  1:3382197/101‑1 (MQ=255)


                                                                         tgtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGa                        >  2:700817/1‑101 (MQ=255)


                                                                          gtCGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGAt                       >  1:315061/1‑101 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  2:269416/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     <  2:4360887/101‑1 (MQ=255)


                                                                            cGATGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATcc                     >  2:4076472/1‑101 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   >  2:3461407/1‑101 (MQ=255)


                                                                              aTGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCgg                   >  2:556413/1‑101 (MQ=255)


                                                                               tGAAAGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGa                  <  2:2936288/101‑1 (MQ=255)


                                                                                 aaaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGaaa                <  2:4022692/101‑1 (MQ=255)


                                                                                 aaaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGaaa                >  1:529388/1‑101 (MQ=255)


                                                                                  aaGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAt               >  2:1505787/1‑101 (MQ=255)


                                                                                   aGCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAAtt              >  2:2167352/1‑101 (MQ=255)


                                                                                    gCAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTc             <  2:2792051/101‑1 (MQ=255)


                                                                                     cAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCa            <  1:1791581/101‑1 (MQ=255)


                                                                                     cAACCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCa            >  2:2235193/1‑101 (MQ=255)


                                                                                      aaCCCCTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCaa           >  2:3137885/1‑101 (MQ=255)


                                                                                        ccccTATGTCGACGCCCGTCTGGCCGAGGGCTCGCGTTTCAACGCGAGGGTGGCTCCGGTCGCGGTGGATGGCTCGCTCGTCGCGACCCGGAAATTCaaga         >  2:3866556/1‑101 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      >  2:979417/1‑101 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      >  2:2552265/1‑101 (MQ=255)


                                                                                           cTATGTCGACGCCCGTCTGGCCGATGGCTCGCGATTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAgg      >  2:2280448/1‑101 (MQ=255)


                                                                                            tATGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGa     >  2:2445134/1‑101 (MQ=255)


                                                                                             aTGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGAc    >  2:6269/1‑101 (MQ=255)


                                                                                              tGTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACa   <  1:2044449/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  2:1925616/101‑1 (MQ=255)


                                                                                               gTCGACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACaa  <  1:1283053/101‑1 (MQ=255)


CGGACGTGACCTTCAAGGATGAAAAGCACCTGTTGCGGATCATCGACAAGATCGTGTCCGCGGTGGGGCGGCGTGTCGATGAAAGCAACCCCTATGTCTACGCCCGTCTGGCCGATGGCTCGCGTTTCAACGCGATGGTGCCCCCGGTCGCGGTGGATGGCTCGCTCGTCTCGATCCGGAAATTCAAGAAGGACAA  >  NC_014034/539645‑539840

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 34 ≤


ATCG

< 41 ≤


ATCG