Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 590,814 C→G 100% Q56E (CAA→GAA)  murI → glutamate racemase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034590,8140C→G100.0% 840.8 224Q56E (CAA→GAA) murIglutamate racemase
Reads supporting (aligned to +/- strand):  new base (114/110):  ref base (0/0):  total (114/110)

Only 100 of 240 total aligned reads displayed.

TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911
                                                                                                  |                                                                                                 
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 >  1:1785836/1‑101 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACGACCTGACCTGCCGCGGCGTCGAAc                                                                                                <  2:4080494/101‑1 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  1:3538299/1‑101 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  1:1308636/1‑101 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  2:789297/1‑101 (MQ=255)
  cccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACg                                                                                               <  2:1308543/101‑1 (MQ=255)
    cGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGc                                                                                             >  1:2886198/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  1:1854153/1‑101 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           >  2:867/1‑101 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           >  2:4385491/1‑101 (MQ=255)
        gTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtgtg                                                                                         >  2:2374371/1‑101 (MQ=255)
           gCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGa                                                                                      >  2:4425192/1‑101 (MQ=255)
            ccTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGAc                                                                                     <  1:1718112/101‑1 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGGCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGGCGAACGGCTGTGGGACg                                                                                    >  1:4146065/1‑101 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGCGGGACg                                                                                    >  1:1817107/1‑101 (MQ=255)
              tttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGa                                                                                   <  1:3471950/101‑1 (MQ=255)
                tGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGGGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgg                                                                                 >  1:4598129/1‑101 (MQ=255)
                    tattatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTg                                                                             >  2:166035/1‑101 (MQ=255)
                    tattatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTg                                                                             >  2:2589721/1‑101 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        <  1:3891255/101‑1 (MQ=255)
                          ggCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCt                                                                       >  2:3529271/1‑101 (MQ=255)
                           gCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTg                                                                      <  1:1656506/101‑1 (MQ=255)
                            cGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGCCCTGCCCAGGCGTCGAACGGCTGTGGGCCGAGGGCTGCGCTCGgg                                                                     >  1:1627293/1‑101 (MQ=255)
                            cGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTgg                                                                     <  2:4574236/101‑1 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTg                                                                   >  1:3850637/1‑101 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTg                                                                   >  2:2635911/1‑101 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGGGCTGGTGa                                                                  >  1:4446124/1‑101 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  1:3930739/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 >  2:3337854/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAt                                                                 >  2:944731/1‑101 (MQ=255)
                                aaTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGGGAt                                                                 >  1:544712/1‑101 (MQ=255)
                                     ccATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTgg                                                            <  2:2308389/101‑1 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           >  1:3281893/1‑101 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           >  2:3378044/1‑101 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           <  2:2387686/101‑1 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           <  2:1785717/101‑1 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          <  1:224877/101‑1 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          <  2:3073148/101‑1 (MQ=255)
                                       aTGCGCCCTACGGCGTGCCACCCCCCGACGTCATCTACACCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          <  2:36559/101‑1 (MQ=255)
                                        tGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCt                                                         <  1:4200768/101‑1 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGGGATTCTGGCCTg                                                        >  1:3370228/1‑101 (MQ=255)
                                          cgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGc                                                       <  1:671834/101‑1 (MQ=255)
                                            cccTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCaa                                                     <  1:852807/101‑1 (MQ=255)
                                             cgtACGCCCTGCGCACCCCCGACCACACCTACACCCTGACCTGCCACCGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    <  2:918711/99‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  1:4503705/101‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   >  2:4394496/1‑101 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   >  2:1080323/1‑101 (MQ=255)
                                               tACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  2:4163433/101‑1 (MQ=255)
                                               tACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  2:1226821/101‑1 (MQ=255)
                                               tACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  2:1854027/101‑1 (MQ=255)
                                               tACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  2:592593/101‑1 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 <  2:661765/101‑1 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 >  2:4444773/1‑101 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGACGCGGCGGCGAACGGCTGCGGGACGAGGGCTGCGAACCGGTGATTCTGGGCTGccccagg                                                 >  2:2957461/1‑95 (MQ=255)
                                                 cGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACgg                                                >  1:1863549/1‑101 (MQ=255)
                                                  ggCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCCACACGGc                                               >  2:173051/1‑101 (MQ=255)
                                                  ggCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGc                                               <  1:2701105/101‑1 (MQ=255)
                                                  ggCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGc                                               >  1:4434921/1‑101 (MQ=255)
                                                   gCGTGCGCACCCCCGACGACATCTACAACCTGGCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGcc                                              <  2:4541594/101‑1 (MQ=255)
                                                      tGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCACCACGGCCTcc                                           >  2:2148679/1‑101 (MQ=255)
                                                      tGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTcc                                           <  1:1412011/101‑1 (MQ=255)
                                                       gcgcACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccg                                          >  2:3894531/1‑101 (MQ=255)
                                                         gcccccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        <  1:780766/98‑1 (MQ=255)
                                                          cccccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgccg                                       <  1:1965490/99‑1 (MQ=255)
                                                           gcccccGATGACTGCTTAAACCTGGCCTGCCGCGGGGTTGAACGTCTGTGGGACGGGGGCTGTGATCTGGTGATTCTGGCCTGCAACACGGCCTCcgccgc                                      <  2:3058379/100‑1 (MQ=255)
                                                               ccGACGACATCTACAACCTGCCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggcg                                  <  1:349816/101‑1 (MQ=255)
                                                               ccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggcg                                  <  1:2412805/101‑1 (MQ=255)
                                                                 gacgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCt                                >  1:2675642/1‑101 (MQ=255)
                                                                 gacgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCt                                <  1:2870226/101‑1 (MQ=255)
                                                                  ttccgaTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  2:3847988/96‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  1:2612923/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               <  2:47148/101‑1 (MQ=255)
                                                                  acgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTg                               >  1:3021224/1‑101 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              <  2:4260036/101‑1 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              >  2:169264/1‑101 (MQ=255)
                                                                    gacATCTACCACCTGGCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  2:2675252/101‑1 (MQ=255)
                                                                    gacATCTACAACCTGACCTGGCGCGGCGTCGAAAGGCTGGGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGAAAAACGGACTCCGCCCCGGCCCTTaa                             >  1:833332/1‑101 (MQ=255)
                                                                     acATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaaa                            >  2:1060131/1‑101 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          >  1:1026505/1‑101 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCAGCGGCGCTGAAAAg                          >  2:3985893/1‑101 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  2:1482702/1‑101 (MQ=255)
                                                                          tACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       >  1:1218916/1‑101 (MQ=255)
                                                                          tACAACCCGACCTGCCGTGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGAt                       <  2:4192424/101‑1 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGagatcggaa                      >  1:3848273/1‑93 (MQ=255)
                                                                           aCAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATg                      >  2:256516/1‑101 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                >  2:4328902/1‑101 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                <  1:4351145/101‑1 (MQ=255)
                                                                                 tgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGACTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                >  1:3135633/1‑101 (MQ=255)
                                                                                   acctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACa              >  1:63853/1‑101 (MQ=255)
                                                                                    cctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  1:4314879/101‑1 (MQ=255)
                                                                                      tgCCGCGGCTCCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           <  2:2740963/101‑1 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           <  2:3020995/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  2:2021117/101‑1 (MQ=255)
                                                                                            ggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATccc     <  1:4167202/101‑1 (MQ=255)
                                                                                            ggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATccc     >  2:3551031/1‑101 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    <  2:544664/101‑1 (MQ=255)
                                                                                             gcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCg    <  2:3201995/101‑1 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  1:3354886/101‑1 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTCGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  1:4000474/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTAGATcggaag  >  1:1153849/1‑96 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG