Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,483 G→A 22.7% I708I (ATC→CGA RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4830G→A22.7% 154.9 ‑3.5 ‑0.1233I708I (ATC→ATTRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (18/35):  ref base (92/88):  total (110/123)
Fisher's exact test strand distribution p-value = 2.96e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.29e-01

Only 100 of 245 total aligned reads displayed.

CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583
                                                                                                    |                                                                                                     
cGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCg                                                                                                       >  1:2412844/1‑101 (MQ=255)
    cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCt                                                                                                  >  2:4324546/1‑101 (MQ=255)
     aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGCGCTGGTCAGCGCGCCG.ATCTg                                                                                                 <  1:2643966/101‑1 (MQ=255)
       cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcg                                                                                               >  1:1460346/1‑101 (MQ=255)
        gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTgcgc                                                                                              <  1:1883206/101‑1 (MQ=255)
         tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                                             >  1:2239848/1‑101 (MQ=255)
         tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                                             <  2:3627433/101‑1 (MQ=255)
         tGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGcc                                                                                             >  2:288156/1‑101 (MQ=255)
          ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                                            >  2:3492193/1‑101 (MQ=255)
          ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCg                                                                                            <  1:4434042/101‑1 (MQ=255)
           gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGa                                                                                           <  1:1736669/101‑1 (MQ=255)
             cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGc                                                                                         >  1:3873790/1‑101 (MQ=255)
              gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                        >  1:3205930/1‑101 (MQ=255)
              gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGcc                                                                                        <  2:2081011/101‑1 (MQ=255)
               agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                       >  2:3802820/1‑101 (MQ=255)
               agcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGccc                                                                                       <  2:1259199/101‑1 (MQ=255)
                gcccagcccGTAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCa                                                                                      <  2:2613461/101‑1 (MQ=255)
                 cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                     <  2:194994/101‑1 (MQ=255)
                 cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAg                                                                                     <  1:1121968/101‑1 (MQ=255)
                   cagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAc                                                                                   <  2:23854/101‑1 (MQ=255)
                   cagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAc                                                                                   >  2:345035/1‑101 (MQ=255)
                   cagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGAc                                                                                   <  2:3572486/101‑1 (MQ=255)
                        cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgcc                                                                              <  2:3065080/101‑1 (MQ=255)
                        cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgcc                                                                              >  1:424765/1‑101 (MQ=255)
                         ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccg                                                                             >  1:4200161/1‑101 (MQ=255)
                         ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccg                                                                             <  1:2775615/101‑1 (MQ=255)
                          gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGcgccgc                                                                            <  2:2897994/101‑1 (MQ=255)
                           agagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCgccgcc                                                                           >  1:2995339/1‑101 (MQ=255)
                            gagCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCt                                                                          <  2:2739261/101‑1 (MQ=255)
                              gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                        <  2:1198459/101‑1 (MQ=255)
                               cgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTgcg                                                                       <  2:2278728/101‑1 (MQ=255)
                                  cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                    <  1:2253307/101‑1 (MQ=255)
                                  cGTCCCGCTGAGCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCCCGCCG.ATCTTCCCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                    <  2:1159285/101‑1 (MQ=25)
                                       cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                               >  2:2981768/1‑101 (MQ=255)
                                       cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                                               <  1:3797802/101‑1 (MQ=255)
                                        gcTGATCTGCGCCAGCTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                              <  1:4392501/101‑1 (MQ=255)
                                        gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                              >  2:177063/1‑101 (MQ=255)
                                        gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                              >  1:2072566/1‑101 (MQ=255)
                                         gtGACCTGCGCCAGCTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAg                                                             <  2:4567994/100‑1 (MQ=255)
                                         cggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAg                                                             <  1:1383044/91‑1 (MQ=2)
                                          tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                                            >  1:4382044/1‑101 (MQ=255)
                                            agccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcgc                                                          <  2:501424/94‑1 (MQ=2)
                                            aTCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTTCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                                          >  1:1087011/1‑101 (MQ=255)
                                            aTCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                                          >  2:1554628/1‑101 (MQ=255)
                                             tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                                         <  1:3668210/101‑1 (MQ=255)
                                             gccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                         <  1:2834991/95‑1 (MQ=2)
                                               tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  2:4500227/101‑1 (MQ=255)
                                               cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                       <  1:1572588/97‑1 (MQ=2)
                                                  cccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                    <  1:870736/100‑1 (MQ=255)
                                                  accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                                                    >  1:1630128/2‑101 (MQ=2)
                                                   ccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                                                   <  1:4506025/101‑1 (MQ=9)
                                                     aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGCTGTc                                                 >  1:1769916/1‑101 (MQ=255)
                                                     aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                 >  1:25612/1‑101 (MQ=255)
                                                       aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGACAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTcct                                               >  2:2318498/1‑99 (MQ=255)
                                                        tCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATc                                              <  1:1105933/101‑1 (MQ=255)
                                                          ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                            >  2:1372868/11‑101 (MQ=9)
                                                          ggcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                            >  1:286960/11‑101 (MQ=9)
                                                          cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                            >  1:2333012/1‑101 (MQ=255)
                                                           gcggtttccaGCGCCGAAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                           <  1:2099066/92‑1 (MQ=9)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         >  2:4067314/8‑101 (MQ=9)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         <  1:4306509/94‑1 (MQ=9)
                                                             ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         >  2:3986805/8‑101 (MQ=9)
                                                             cgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                         <  1:3462265/101‑1 (MQ=255)
                                                              gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  1:570978/95‑1 (MQ=9)
                                                              gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  1:61100/95‑1 (MQ=9)
                                                              gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        >  2:773499/7‑101 (MQ=9)
                                                              gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                        <  1:262082/95‑1 (MQ=9)
                                                                ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCTCCACGATGTCATCGGTGccga                                      <  2:219624/97‑3 (MQ=9)
                                                                 tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCTAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                     <  1:3924149/101‑1 (MQ=255)
                                                                  ccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgagg                                    <  2:332547/99‑5 (MQ=9)
                                                                   gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc                                   <  2:2770451/101‑1 (MQ=255)
                                                                    ggcgcCGCGACCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                  <  2:215413/100‑1 (MQ=255)
                                                                    aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                  <  2:3846082/101‑1 (MQ=255)
                                                                    aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                  >  2:1431597/1‑101 (MQ=255)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  >  2:2794383/1‑101 (MQ=2)
                                                                    aGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcc                                  >  1:1259292/1‑101 (MQ=2)
                                                                      cgccgcAACCTGCGTGCGCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                <  2:867686/101‑3 (MQ=2)
                                                                       gccgcGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                               >  2:2504900/1‑101 (MQ=255)
                                                                       gccgcGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcgcg                               >  2:4420740/1‑101 (MQ=255)
                                                                       gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                               <  2:3292091/101‑4 (MQ=9)
                                                                       gccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                               <  2:3957563/101‑4 (MQ=9)
                                                                       cccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt                               <  2:2828514/100‑4 (MQ=2)
                                                                        ccgcGATCTGCGCCGTGCTCAGCGCCGCG.ATCTGCGTCGCGCTCAGCCGTGCCGCCTGCGCCGTGGTCAGCGCCACGATGTAATCGGCGCCaagttccgag                              >  1:2179183/1‑93 (MQ=21)
                                                                        ccgcAACCTGCGTGCTCGACAGCGCCAGA.AGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                              <  1:1530769/101‑5 (MQ=2)
                                                                           cgATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc                           <  2:2591340/101‑1 (MQ=255)
                                                                            gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          >  1:814188/1‑101 (MQ=255)
                                                                            gATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                          <  2:3503218/101‑1 (MQ=255)
                                                                             aTCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgc                         <  1:2143054/101‑1 (MQ=255)
                                                                              tCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                        <  2:2681195/101‑1 (MQ=255)
                                                                               cTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                       >  1:3959631/1‑101 (MQ=255)
                                                                                  cGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccg                    >  2:418058/1‑101 (MQ=255)
                                                                                   gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGCCGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                   <  1:4079524/101‑1 (MQ=255)
                                                                                   gTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                   <  2:622120/101‑1 (MQ=255)
                                                                                     gCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGc                 <  1:3611068/101‑1 (MQ=255)
                                                                                      cTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                >  1:2790705/1‑101 (MQ=255)
                                                                                       tGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc               >  1:914778/1‑101 (MQ=255)
                                                                                          tCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgc            <  2:4240443/101‑1 (MQ=255)
                                                                                              cccgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccg        <  2:1032194/99‑1 (MQ=255)
                                                                                               gcgcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc       >  1:752865/1‑101 (MQ=255)
                                                                                                 gcCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCa     >  1:4553438/1‑101 (MQ=255)
                                                                                                    |                                                                                                     
CGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.ATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCG  >  NC_014034/681383‑681583

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG