BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	682,521	G→C	6.5%	A362A (GCC→GCG)	RCAP_rcc00630 ←	ice nucleation protein repeat family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	682,521	0	G→C	6.5%	24.5 ‑1.2 0.0	232	A362A (GCC→GCG)	RCAP_rcc00630	ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand): new base (5/11): ref base (101/115): total (106/126)
Fisher's exact test strand distribution p-value = 3.01e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.


TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGG  >  NC_014034/682421‑682621


tCTGCGCGGAGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTgg                                                                                                       >  2:2275515/1‑101 (MQ=255)


   gcgcGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGcc                                                                                                    <  1:4516979/101‑1 (MQ=255)


    cgcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCg                                                                                                   >  1:1818020/1‑101 (MQ=255)


     gcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGt                                                                                                  <  1:105822/101‑1 (MQ=255)


     gcgGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGt                                                                                                  <  1:349804/101‑1 (MQ=255)


       ggTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGc                                                                                                >  1:2298802/1‑101 (MQ=255)


         tGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGCCAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTc                                                                                              <  1:3948518/101‑1 (MQ=255)


          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCa                                                                                             >  1:3154842/1‑101 (MQ=255)


          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCa                                                                                             <  2:1943048/101‑1 (MQ=255)


          ggACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCa                                                                                             <  1:4635673/101‑1 (MQ=255)


            aCAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgc                                                                                           >  2:4309187/1‑101 (MQ=255)


              aaGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAgcgc                                                                                         >  2:3291211/1‑101 (MQ=255)


               aGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGcgcc                                                                                        >  1:232939/1‑101 (MQ=255)


                ggCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGcgccg                                                                                       <  2:1536580/101‑1 (MQ=255)


                 gCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCCGcgccgc                                                                                      >  1:4379261/1‑101 (MQ=255)


                  cGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCgccgcc                                                                                     <  2:827812/101‑1 (MQ=255)


                   gTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCa                                                                                    <  1:4118037/101‑1 (MQ=255)


                   gTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCa                                                                                    >  2:3682818/1‑101 (MQ=255)


                    tCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCAt                                                                                   <  1:4480195/101‑1 (MQ=255)


                    ccGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCAt                                                                                   >  1:574491/2‑101 (MQ=255)


                     cGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGGCAGCGCCGCGATCTGCCGCGGGCCCAGCACACCCAGCTGGGCCGTGCTCAGCGCCGCCATc                                                                                  >  1:3861893/1‑101 (MQ=255)


                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                 >  2:1279654/1‑101 (MQ=255)


                      gATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCt                                                                                 >  1:2141345/1‑101 (MQ=255)


                       aTCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTg                                                                                <  2:3358883/101‑1 (MQ=255)


                       aTCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTg                                                                                <  2:2461644/101‑1 (MQ=255)


                        tCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgc                                                                               >  1:1429165/1‑101 (MQ=255)


                          tGCGCCGAACTCAGCGCCTCGGCTGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTgcgc                                                                             <  1:692559/101‑1 (MQ=255)


                           gcgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGcc                                                                            <  2:1792520/101‑1 (MQ=255)


                            cgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGccc                                                                           >  2:1298482/1‑101 (MQ=255)


                            cgcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGccc                                                                           <  2:1457055/101‑1 (MQ=255)


                             gcCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGcccc                                                                          <  2:648103/101‑1 (MQ=255)


                              ccGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCg                                                                         <  1:2002605/101‑1 (MQ=255)


                              ccGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCg                                                                         <  2:3001622/101‑1 (MQ=255)


                                gAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGt                                                                       >  1:985015/1‑101 (MQ=255)


                                  aCTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCa                                                                     <  1:2874087/101‑1 (MQ=255)


                                   nncNNNGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATGTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAg                                                                    <  1:1286803/99‑1 (MQ=255)


                                   cTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAg                                                                    <  1:4610970/101‑1 (MQ=255)


                                    tCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgc                                                                   >  1:394141/1‑101 (MQ=255)


                                     cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                  <  2:4324085/101‑1 (MQ=255)


                                     cAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAgcg                                                                  >  2:493618/1‑101 (MQ=255)


                                      aGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGAGCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGGGCCGCCATCTGCGCCCCGGTCCgcgc                                                                 >  2:3744789/1‑101 (MQ=255)


                                       gcgcCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGcgcc                                                                <  2:2918065/101‑1 (MQ=255)


                                          ccTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCgccgcc                                                             >  2:2794850/1‑101 (MQ=255)


                                           cTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCa                                                            <  1:814035/101‑1 (MQ=255)


                                              ggcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGtt                                                         <  2:4331708/101‑1 (MQ=255)


                                               gcggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTc                                                        <  1:2619489/101‑1 (MQ=255)


                                                cggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCg                                                       >  2:1109051/1‑101 (MQ=255)


                                                 ggcCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGa                                                      >  1:219581/1‑101 (MQ=255)


                                                   cctcctCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATc                                                    >  1:2949905/1‑101 (MQ=255)


                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCTGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                             <  2:4035302/101‑1 (MQ=255)


                                                         cGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTc                                             <  1:3402281/101‑1 (MQ=255)


                                                          ggACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCa                                            >  1:3720385/1‑101 (MQ=255)


                                                            aCAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGc                                          >  2:289818/1‑101 (MQ=255)


                                                            aCAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGc                                          >  2:905406/1‑101 (MQ=255)


                                                             cAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGcc                                         <  2:4488560/101‑1 (MQ=255)


                                                                cgccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCgg                                      >  1:944043/1‑101 (MQ=255)


                                                                 gccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                     >  1:3025691/1‑101 (MQ=255)


                                                                 gccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGc                                     >  2:2431900/1‑101 (MQ=255)


                                                                  ccgcGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGcc                                    <  1:361754/101‑1 (MQ=255)


                                                                   cgcgATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCa                                   <  2:696690/101‑1 (MQ=255)


                                                                    gcgATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCAt                                  <  2:2068890/101‑1 (MQ=255)


                                                                       aTCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTc                               <  2:1166633/101‑1 (MQ=255)


                                                                        tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                              <  2:2534291/101‑1 (MQ=255)


                                                                          tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                            >  1:4225942/1‑101 (MQ=255)


                                                                           gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                           <  2:2682811/101‑1 (MQ=255)


                                                                           gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                           >  1:1787036/1‑101 (MQ=255)


                                                                           gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                           <  1:88971/101‑1 (MQ=255)


                                                                             cgcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                         <  1:255225/101‑1 (MQ=255)


                                                                              gcgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTc                        <  1:1445328/101‑1 (MQ=255)


                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                       >  2:676257/1‑101 (MQ=255)


                                                                               cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                       <  1:748541/101‑1 (MQ=255)


                                                                                gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                      >  2:219567/1‑101 (MQ=255)


                                                                                gTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGa                      >  1:2893787/1‑101 (MQ=255)


                                                                                 tGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaa                     <  2:1429070/101‑1 (MQ=255)


                                                                                   cccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAg                   <  1:1109140/101‑1 (MQ=255)


                                                                                    ccAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGc                  >  2:1359922/1‑101 (MQ=255)


                                                                                      aGAGCCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGccc                <  1:1493073/101‑1 (MQ=255)


                                                                                       gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc               <  2:23373/101‑1 (MQ=255)


                                                                                        cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt              <  1:1003370/96‑1 (MQ=37)


                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt             <  2:3735809/97‑1 (MQ=37)


                                                                                         gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt             >  1:135916/5‑101 (MQ=37)


                                                                                         cccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt             <  2:729992/97‑1 (MQ=37)


                                                                                          ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg            <  1:1718649/98‑2 (MQ=37)


                                                                                          cccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTc            <  1:45412/101‑1 (MQ=255)


                                                                                           cgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttga           <  2:166127/99‑3 (MQ=37)


                                                                                           ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa           <  2:1114567/101‑1 (MQ=255)


                                                                                           ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa           <  2:26307/101‑1 (MQ=255)


                                                                                           ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa           >  1:2760362/1‑101 (MQ=255)


                                                                                           ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa           <  2:3855833/101‑1 (MQ=255)


                                                                                             ccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAgc         <  1:2909646/101‑1 (MQ=25)


                                                                                               aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc       >  2:309080/1‑101 (MQ=255)


                                                                                               aGCTGGGCCGGGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc       <  2:2170620/101‑1 (MQ=255)


                                                                                                gCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGcgcg      <  1:1920199/101‑1 (MQ=255)


                                                                                                 cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg     >  2:233943/1‑101 (MQ=255)


                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    >  1:533009/1‑101 (MQ=255)


                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    >  2:1776078/1‑101 (MQ=255)


                                                                                                  tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc    >  1:2674214/1‑101 (MQ=255)


                                                                                                   gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg   >  2:4202297/1‑101 (MQ=255)


                                                                                                   gggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggcg   <  1:4052861/101‑1 (MQ=255)


                                                                                                    ggCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGcggcgg  >  1:657399/1‑101 (MQ=255)


TCTGCGCGGTGGACAAGGCGTCGATCTGCGCCGAACTCAGCGCCTCGGCGGCCTCCTCGGACAGCGCCGCGATCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGG  >  NC_014034/682421‑682621

Base quality scores:


ATCG

< 3 ≤


ATCG

< 20 ≤


ATCG

< 29 ≤


ATCG

< 33 ≤


ATCG

< 40 ≤


ATCG