Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 774,645 G→C 100% intergenic (‑99/+21) oppB1 ← / ← oppA1 oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034774,6450G→C100.0% 96.8 25intergenic (‑99/+21)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (23/2):  ref base (0/0):  total (23/2)

GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774597‑774728
                                                    |                                                                                   
catcaacggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGtt                                   >  2:3061707/9‑101 (MQ=255)
caggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGtt                                   >  2:284403/9‑101 (MQ=255)
 aggcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTc                                  >  2:3133495/8‑101 (MQ=255)
   gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa                                >  2:3819158/6‑101 (MQ=255)
       ggcgcCCCCCCGCCCGGGTCGGACCGGCCCTG.CCGGTCCG.ACCCCACGGTATCTTATCCTGCGATCACTTCGTCCGCGACCAGTCGGCAACGTTCCAGTAt                            >  2:478285/2‑101 (MQ=255)
       ggcgcCCCCCCGCCCGGGGCGGACCGGCCCCGCCCGGC.CGCC.CCCACGGTCTCTTATCCTGCGATCACTTCGCCCCCGACCCGGCGGCCACGTTcccggat                            >  1:390461/2‑96 (MQ=255)
          gcCCCCCCGCCCGGGTCGGACGGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCCCGTTCCCGGATTCg                         >  1:2650545/1‑101 (MQ=255)
                    ccgggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAgg               >  2:1796092/10‑101 (MQ=255)
                     cgggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc              >  1:1798786/9‑101 (MQ=255)
                      gggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg             >  2:2565656/8‑101 (MQ=255)
                      gggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCg             <  1:4166934/94‑1 (MQ=255)
                       ggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  1:2311324/7‑101 (MQ=255)
                       ggtcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt            >  1:802062/7‑101 (MQ=255)
                         tcggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc          >  2:3307971/5‑101 (MQ=255)
                          cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCNCCTNCCAGTATTCGCNGTCCCAGGCGTTCa         >  1:4266375/4‑101 (MQ=255)
                          cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  2:265685/4‑101 (MQ=255)
                          cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:999618/4‑101 (MQ=255)
                          cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:474176/4‑101 (MQ=255)
                          cggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa         >  1:1628304/4‑101 (MQ=255)
                           ggaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt        >  2:968851/3‑101 (MQ=255)
                            gaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  2:3677160/2‑101 (MQ=255)
                            gaCCGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc       >  1:2049142/2‑101 (MQ=255)
                              ccGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTGCCAGTATTCGCTGTCCCAGGCGTTCAcctc     >  1:278068/1‑101 (MQ=255)
                               cGGCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCg    >  1:3306738/1‑101 (MQ=255)
                                ggCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:2509242/1‑101 (MQ=255)
                                ggCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGa   >  2:1387819/1‑101 (MQ=255)
                                 gCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  <  1:3958314/101‑1 (MQ=255)
                                 gCCCTG.CCGGTCCG.ACCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc  >  1:1068352/1‑101 (MQ=255)
                                                    |                                                                                   
GGGCCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTG.CCGGT.CG.A.CGTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAC  >  NC_014034/774597‑774728

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG